hygiena KIT230228 Duck Species Detection Kit User Guide
- May 15, 2024
- Hygiena
Table of Contents
hygiena KIT230228 Duck Species Detection Kit
Specifications
- Product Name: Duck Species Detection Kit
- Product No.: KIT230228
- Revision: A, December 2023
- Application: PCR kit for the qualitative detection of Duck DNA using real-time PCR instruments
Product Usage Instructions
Program Setup
Before setting up the PCR reactions, program your real-time PCR instrument with the following settings:
- Channels: FAM (Duck) and VIC/HEX (Internal Control)
- Program:
- 10 minutes, 1 cycle
- 15 seconds | 40 seconds, 35 cycles (Fluorescence detection)
Data Interpretation
- Verify results of positive (Control Template) and negative (H2O) controls before interpreting sample results.
- Always compare samples to positive and negative controls.
- Interpret results based on the data from each channel as described in the table:
| FAM | VIC/HEX | Result Interpretation |
|---|---|---|
| + | – | Positive for Duck |
| + | or + | Negative for Duck |
| – | – | Invalid |
Preparation of the RT-PCR Mix
Take precautions to prevent contamination by using filter tips and wearing gloves.
Follow these steps:
- Prepare PCR Mix: Prepare for each reaction in a suitable tube. Mix carefully but thoroughly by pipetting up and down.
- Add PCR Mix.
- Add Samples and Controls.
- Seal: Seal strips/plate accurately.
- Centrifuge: Briefly spin strips/plate in a suitable centrifuge.
- Start Real-time PCR Run: Cycle samples as described in the program setup.
For food testing purposes. FOR IN VITRO USE ONLY.
FAQ
Q: Can this kit be used for species other than Duck?
- A: No, this kit is specifically designed for the qualitative detection of Duck DNA only.
Q: How should I store the kit when not in use?
- A: Store the kit components according to the instructions provided in the product manual to maintain their integrity.
Product No. KIT230228
- PCR kit for the qualitative detection of Duck DNA using real-time PCR instruments.
- Before starting, it is strongly recommended to read the entire product manual available on our website.
PROGRAM SETUP
- Program your real-time PCR instrument before setting up the PCR reactions. Select the following channels:
- FAM (Duck) and VIC/HEX (Internal Control).
- Pre-incubation: 1 cycle
- Step 1: 95 °C for 10 min
- Amplification: 35 cycles
- Step 1: 95 °C for 15 sec
- *Step 2:** 61 °C for 40 sec
- Fluorescence detection
DATA INTERPRETATION
- Verify the results of positive (Control Template) and negative (H2O) controls, before interpreting the sample results.
- Always compare samples to positive and negative controls.
- Review data from each channel and interpret results as described in the table.
| FAM | VIC/HEX | Result Interpretation |
|---|---|---|
| + | + or – | Positive for Duck |
| – | + | Negative for Duck |
| – | – | Invalid |
PREPARATION OF THE RT-PCR MIX
- Take appropriate precautions to prevent contamination, e.g., using filter tips and gloves.
- Thaw reagents, mix (do not vortex!), and briefly spin vials before opening.
- PREPARE PCR MIX
- Add 10 μL Master Mix (2XM) and for each reaction to 4 μL Primer/Probe Mix (P) a suitable tube.
- (n samples + 2 controls + at least one additional reaction to cover pipetting loss). Mix carefully but thoroughly by pipetting up and down.
- ADD PCR MIX
- Pipet 14 μL of prepared PCR mix into each strip or plate well.
- ADD SAMPLES AND CONTROLS
- Pipet 6 µL of samples, negative control (PCR-grade H2O, not included) or Control Template (C) into respective wells.
- SEAL
- Seal strips/plates accurately.
- CENTRIFUGE
- Briefly spin strips/plate in a suitable centrifuge.
- START REAL-TIME PCR RUN
- Cycle samples as described above.
- foodproof®
- SL DuckSpecies Detection Kit
- KIT230228
- Kit for 50 reactions Store kit at -15 to -25 °C
- For food testing purposes
- FOR IN VITRO USE ONLY
- Hygiena®
- Camarillo, CA 93012 USA
- diagnostics.support@hygiena.com
- www.hygiena.com
Documents / Resources
|
hygiena KIT230228 Duck Species Detection
Kit
[pdf] User Guide
KIT230228, KIT230228 Duck Species Detection Kit, Duck Species Detection Kit,
Species Detection Kit, Detection Kit, Kit
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References
Read User Manual Online (PDF format)
Read User Manual Online (PDF format) >>