hygiena KIT230228 Duck Species Detection Kit User Guide

hygiena KIT230228 Duck Species Detection Kit

Specifications

• Product Name: Duck Species Detection Kit
• Product No.: KIT230228
• Revision: A, December 2023
• Application: PCR kit for the qualitative detection of Duck DNA using real-time PCR instruments

Product Usage Instructions

Program Setup

Before setting up the PCR reactions, program your real-time PCR instrument with the following settings:

• Channels: FAM (Duck) and VIC/HEX (Internal Control)
• Program:
  • 10 minutes, 1 cycle
  • 15 seconds | 40 seconds, 35 cycles (Fluorescence detection)

Data Interpretation

• Verify results of positive (Control Template) and negative (H2O) controls before interpreting sample results.
• Always compare samples to positive and negative controls.
• Interpret results based on the data from each channel as described in the table:

Preparation of the RT-PCR Mix

Take precautions to prevent contamination by using filter tips and wearing gloves.

Follow these steps:

1. Prepare PCR Mix: Prepare for each reaction in a suitable tube. Mix carefully but thoroughly by pipetting up and down.
2. Add PCR Mix.
3. Add Samples and Controls.
4. Seal: Seal strips/plate accurately.
5. Centrifuge: Briefly spin strips/plate in a suitable centrifuge.
6. Start Real-time PCR Run: Cycle samples as described in the program setup.

For food testing purposes. FOR IN VITRO USE ONLY.

FAQ

Q: Can this kit be used for species other than Duck?

• A: No, this kit is specifically designed for the qualitative detection of Duck DNA only.

Q: How should I store the kit when not in use?

• A: Store the kit components according to the instructions provided in the product manual to maintain their integrity.

Product No. KIT230228

• PCR kit for the qualitative detection of Duck DNA using real-time PCR instruments.
• Before starting, it is strongly recommended to read the entire product manual available on our website.

PROGRAM SETUP

• Program your real-time PCR instrument before setting up the PCR reactions. Select the following channels:
• FAM (Duck) and VIC/HEX (Internal Control).
• Pre-incubation: 1 cycle
  • Step 1: 95 °C for 10 min
• Amplification: 35 cycles
  • Step 1: 95 °C for 15 sec
• *Step 2:** 61 °C for 40 sec
  • Fluorescence detection

DATA INTERPRETATION

• Verify the results of positive (Control Template) and negative (H2O) controls, before interpreting the sample results.
• Always compare samples to positive and negative controls.
• Review data from each channel and interpret results as described in the table.

PREPARATION OF THE RT-PCR MIX

• Take appropriate precautions to prevent contamination, e.g., using filter tips and gloves.
• Thaw reagents, mix (do not vortex!), and briefly spin vials before opening.

1. PREPARE PCR MIX
   • Add 10 μL Master Mix (2XM) and for each reaction to 4 μL Primer/Probe Mix (P) a suitable tube.
   • (n samples + 2 controls + at least one additional reaction to cover pipetting loss). Mix carefully but thoroughly by pipetting up and down.
2. ADD PCR MIX
   • Pipet 14 μL of prepared PCR mix into each strip or plate well.
3. ADD SAMPLES AND CONTROLS
   • Pipet 6 µL of samples, negative control (PCR-grade H2O, not included) or Control Template (C) into respective wells.
4. SEAL
   • Seal strips/plates accurately.
5. CENTRIFUGE
   • Briefly spin strips/plate in a suitable centrifuge.
6. START REAL-TIME PCR RUN
   • Cycle samples as described above.

• foodproof®
  • SL DuckSpecies Detection Kit
• KIT230228
  • Kit for 50 reactions Store kit at -15 to -25 °C
• For food testing purposes
  • FOR IN VITRO USE ONLY
• Hygiena®
• Camarillo, CA 93012 USA
• diagnostics.support@hygiena.com
• www.hygiena.com

Documents / Resources

| hygiena KIT230228 Duck Species Detection Kit [pdf] User Guide
KIT230228, KIT230228 Duck Species Detection Kit, Duck Species Detection Kit, Species Detection Kit, Detection Kit, Kit
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References

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