hygiena MON810 GMO Maize Detection Kit User Guide

hygiena MON810 GMO Maize Detection

Specifications

• Product Name: GMO MON810 Maize Detection Kit
• Product No.: KIT230205
• Revision: A, December 2023
• PCR kit for the qualitative detection of MON810 DNA using real-time PCR instruments
• Kit for 50 reactions
• For food testing purposes
• For in vitro use only

Product Usage Instructions

Program Setup

1. Program your real-time PCR instrument before setting up the PCR reactions.
   • Select   the following channels: FAM (MON810) and VIC/HEX (Internal Control).

Data Interpretation

1. Verify results of positive (Control Template) and negative (H2O) controls before interpreting sample results.
2. Always compare samples to positive and negative controls. Review data from each channel and interpret results as described in the table provided.

Preparation of the PCR Mix

1. Take appropriate precautions to prevent contamination, e.g., by using filter tips and wearing gloves.
2. Thaw reagents, mix (do not vortex!) and briefly spin vials before opening.
3. Prepare PCR mix for each reaction in a suitable tube, ensuring cover pipetting loss.
4. Mix carefully but thoroughly by pipetting up and down.
5. Add PCR mix, samples, and controls to the designated tubes.
6. Carefully seal strips/plates and briefly spin them in a suitable centrifuge.
7. Start the real-time PCR run following the cycle samples as described in the manual.

FAQ

Q: Can this kit be used for human DNA testing?

• A: No, this kit is designed for the qualitative detection of MON810 DNA in maize samples and is intended for food testing purposes only.

Q: How should I store the kit when not in use?

• A: Store the kit as per the storage instructions provided in the product manual to maintain the integrity of the reagents.
• Product No. KIT230205
• PCR kit for the qualitative detection of MON810 DNA using real-time PCR instruments.
• Before starting, it is strongly recommended to read the entire product manual available on our website.

PROGRAM SETUP

• Program your real-time PCR instrument before setting up the PCR reactions.

Select the following channels:

• FAM (MON810) and VIC/HEX (Internal Control).
• Pre-incubation: 1 cycle
  • Step 1: 50 °C for 2 min
  • Step 2: 95 °C for 10 min
• Amplification: 45 cycles
  • Step 1: 95 °C for 15 sec
  • Step 2: 60 °C for 60 sec
• Fluorescence detection

DATA INTERPRETATION

• Verify the results of positive (Control Template) and negative (H2O) controls, before interpreting the sample results.
• Always compare samples to positive and negative controls.
• Review data from each channel and interpret results as described in the table.

PREPARATION OF THE PCR MIX

• Take appropriate precautions to prevent contamination, e.g., using filter tips and gloves.
• Thaw reagents, mix (do not vortex!), and briefly spin vials before opening.

1. PREPARE PCR MIX
   • Add 12.5 µL Master Mix (2XM) , for each reaction to 4.0 μL Primer/Probe Mix (P) and a suitable tube.
   • 3.5 μL PCR-grade H2O (not included) (n samples + 2 controls + at least one additional reaction to cover pipetting loss).
   • Mix carefully but thoroughly by pipetting up and down.
2. ADD PCR MIX
   • Pipette 20 µL of prepared PCR mix into each strip or plate well.
3. ADD SAMPLES AND CONTROLS
   • Pipette 5 µL of samples, negative control (PCR-grade H2O), or Control Template (C) into respective wells.
4. SEAL
   • Carefully seal strips/plates.
5. CENTRIFUGE
   • Briefl y spin strips/plate in a suitable centrifuge.
6. START REAL-TIME PCR RUN
   • Cycle samples as described above.

• foodproof® SL GMO MON810 Maize Detection Kit
• KIT230205
• Kit for 50 reactions Store kit at -15 to -25 °C
• For food testing purposes FOR IN VITRO USE ONLY
• Hygiena®
• Camarillo, CA 93012 USA
• diagnostics.support@hygiena.com
• www.hygiena.com

Documents / Resources

| hygiena MON810 GMO Maize Detection Kit [pdf] User Guide
MON810, MON810 GMO Maize Detection Kit, GMO Maize Detection Kit, Maize Detection Kit, Detection Kit, Kit
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References

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