hygiena KIT230016 GMO CaMV Detection Kit User Guide
- March 13, 2024
- Hygiena
Table of Contents
hygiena KIT230016 GMO CaMV Detection Kit User Guide
Product No. KIT230016 :Revision A, December 2023
PCR kit for the qualitative detection of CaMV DNA using real-time PCR instruments. Before starting, it is strongly recommended to read the entire product manual available on our website
PCR kit for the qualitative detection of CaMV DNA using real-time PCR instruments. Before starting, it is strongly recommended to read the entire product manual available on our website
PROGRAM SETUP
Program your real-time PCR instrument before setting up the PCR reactions.
Select the following channels:
Pre-incubation: 1 cycle
Step 1: 37 °C for 4 min
Step 2: 95 °C for 10 min
Amplifi cation: 50 cycles
Step 1 : 95 °C for 15* s
Step 2:** 60 °C for 60 s
For some real-time PCR instruments the probe quencher as well as the usage of a passive reference dye has to be specified. This kit contains probes with a non-fluorescent “dark” quencher and no passive reference dye.
DATA INTERPRETATION
Verify results of positive (Control Template) and negative controls (H2 O), before interpreting sample results. Always compare samples to positive and negative control. Review data from each channel and interpret results as described in the table.
| FAM | Result Interpretation |
|---|---|
| + | Positive for CaMV |
| – | Negative for CaMV* |
The assay is used in addition to the foodproof GMO Screening LyoKits and does not contain an internal amplification control. Please check screening results for inhibition control.
PREPARATION OF THE PCR MIX
Take appropriate precautions to prevent contamination, e.g., by using fi lter tips and wearing gloves. Thaw reagents, mix (do not vortex!), and briefl y spin vials before opening.
-
ADD PCR MIX
Pipet 20 µL of Master Mix into each strip or plate well (n samples + 2 controls). -
ADD SAMPLES AND CONTROLS
Pipet 5 µL of samples, negative control (colorless cap) or Control Template (purple cap) into respective wells. -
SEAL
Seal strips/plate accurately -
CENTRIFUGE
Briefl y spin strips/plate in a suitable centrifuge. -
START REAL-TIME PCR RUN
Cycle samples as described above.
foodproof®
CaMV Detection Kit
KIT230016
Kit for 96 reactions Store kit at -15 to -25 °C
For food testing purposes FOR IN VITRO USE ONLY
Made in Germany
Hygiena® | Camarillo, CA 93012 USA |
diagnostics.support@hygiena.com |
www.hygiena.com