euromex IS.1153-PLPHi iScope Microscope User Manual

euromex IS.1053-PLPOLi iScope Trinocular Polarizing Microscope

Introduction

Thank you for purchasing the Euromex iScope. The iScope series has been designed with all kind of Life Sciences applications and great durability in mind. This resulted in a modern, robust and high level microscope for everyday use, equipped with excellent optical and mechanical components. Specific attention to production methods resulted also in an excellent price/performance ratio. Please read this manual carefully before using this product to ensure correct and safe usage

• The content of this manual is subject to change without notice
• The appearance of the actual product can differ from the models described in this manual
• Not all equipment mentioned in this manual has to be part of the set you have purchased

General safety instructions

Intended use: as a non-medical device
This microscope is intended for general observation of cells and tissues. The microscope is intended to be used with transmitted/reflected illumination and with the specimen fixed on a slide

Intended use: as a medical device class I
This microscope is intended for observation and diagnostics of cells and tissues at hospitals or by physicians and veterinaries in private practice in pathology, anatomy and cytology applications. To be used with transmitted/ reflected illumination and with the specimen fixed on a slide. Physicians and veterinaries use microscopes to identify the different types of cells and spot abnormal cells. This product helps in identifying and treating diseases

Dangers associated with the operation

• Improper use could result in injury, malfunction or damage to property. It must be ensured that the operator informs every user of existing hazards
• Danger of electrocution. Disconnect the power to the entire lighting system before installing, adding or changing any component
• Not to be used in corrosive or explosive environments
• Avoid direct exposure of eyes to the collimated light beam or direct light from the light guides or fibres
• To avoid a hazard to children, account for all parts and keep all packing materials in a safe placev

Photobiological safety LED, important safety instructions

• Avoid direct eye exposure to any LED light source while it is on
• Before looking into the eyepieces of the microscope, lower the intensity of the LED illumination to a low level
• Avoid high-intensity exposure and long exposure to LED light because this can cause acute damage to the retina of the eye

Prevention of biological and infectious hazards
Infectious or bacterial or viral biohazard substances under observation may be a risk to the health of humans and other living organisms. Special precautions should be taken during in vitro medical procedures:

• Biological hazards: keep a logbook of all the biological substances or pathogenic microorganisms that were under observation with the microscope and show it to everybody before they use the microscope or before they do some maintenance work on the microscope! Agents can be bacterial, spores, enveloped or non­enveloped virus particles, fungi or protozoa

• Contamination hazard:

• A sample that is properly enclosed with a cover glass, never comes in direct contact with the microscope parts. In that case prevention of contamination lies in the handling of the slides, as long as the slides are decontaminated before use and are treated normally and are not damaged, there is virtually zero risk of contamination

• A sample that is mounted on a slide without cover glass, can come in contact with components of the microscope and be a hazard to humans and/or the environment. Therefore, check the microscope and accessories on possible contaminations. Clean the microscope surfaces and its components as thoroughly as possible and should you identify a possible contamination, inform the local responsible person in your organisation

• Microscope operators could be contaminated from other activities and cross-contaminate components
  of the microscope. Therefore, check the microscope and accessories on possible contaminations. Clean the microscope surfaces and its components as thoroughly as possible and should you identify a possible contamination, inform the local responsible person in your organisation. It’s recommended to wear sterile gloves when preparing the slides and manipulating the microscope in order to reduce contamination by the operator

• Infection hazard: direct contact with the focusing knobs, stage adjustments, stage and eyepieces/tubes of the microscope can be a potential source of bacterial and/or viral infections. The risk can be limited by using personal eyeshades or eyepieces. You can also use personal protections such as operation gloves and/or safety goggles which can be frequently changed to minimize the risk

• Disinfectant hazards: before cleaning or disinfection check if the room is adequately ventilated. If not, wear respiratory protective equipment. Exposure to chemicals and aerosols can harm human eyes, skin and respiratory system. Do not inhale vapours. During disinfection, do not eat, drink or smoke. Used disinfectants must be disposed according to local or national regulations for health and safety

1.4 Disinfection and decontamination

• exterior casing and mechanical surfaces must be wiped with a clean cloth dampened with a disinfectant
• soft plastic parts and rubber surfaces can be cleaned by gently wiping a clean cloth dampened with a disinfectant. Discoloration can occur if alcohol is used
• the front lens of eyepieces and objectives are sensitive to chemicals. We recommend not to use aggressive disinfectants but to use lens paper or a soft fibre-free tissue damped in cleaning solution. Cotton swabs can also be used. We recommend you use personal eyepieces without eyeshades in order to minimize risk
• never immerse or dip the eyepiece or objective into a disinfectant liquid! This will damage the component
• never use abrasive compounds or cleaners that can damage and scratch coating surfaces of optics
• Clean and disinfect all possible contaminated surfaces of the microscope or contaminated accessories properly before storing for future use. Disinfection procedures must be effective and appropriate
• Leave the disinfectant on the surface for the required exposure time, as specified by the manufacturer. If the disinfectant evaporates before the full exposure time, reapply disinfectant on the surface
• For disinfection against bacteria, use a 70% aqueous solution of isopropanol (isopropyl alcohol) and apply for at least 30 seconds. Against viruses, we recommend to refer to specific alcohol or non-alcohol based disinfection products for laboratories

Before returning a microscope for repair or maintenance through a Euromex dealer, a RMA (return authorization form) and a decontamination statement must be filled in! This document – available from Euromex for any reseller-must be shipped together at all times with the microscope

Reference documents

World Health Organisation:
https://www.who.int/ihr/publications/biosafety-video-series/en/
Robert Koch lnstitut:
https://lin k. springer.co m/content/pdf/10.1007 /sOO 103-013-1863-6. pdf
US Centre for Disease Control and prevention
https://www.cdc.gov/infectioncontrol/guidelines/disinfection/index.html
Handle with care

• This product is a high quality optical instrument. Delicate handling is required
• Avoid subjecting it to sudden shocks and impacts
• Impacts, even small ones, can affect the precision of the objective

Handling the LED
Note: Always disconnect the power cord from your microscope before handling the LED bulb and power unit and allow the system approximately 35 minutes to cool down to avoid burns

• Never touch the LED with your bare hands
• Dirt or fingerprints will reduce the life span and can result in uneven illumination lowering the optical performance
• Use only Euromex original replacement LEDs
• Use of other products will cause malfunctions and void warranty
• During use of the microscope the power unit will get hot; never touch it while in operation and allow the system approximately 35 minutes to cool down to avoid burns

Dirt on the lenses

• Dirt on or inside the optical components, such as eyepieces, lenses, etc., affect the image quality of your system negatively
• Always try to prevent your microscope from getting dirty by using the dust cover, prevent leaving fingerprints on the lenses and clean the outer surface of the lens regularly
• Cleaning optical components is a delicate matter. Please read the cleaning instructions further on in this manual

Environment, storage and use

• This product is a precision instrument and it should be used in a proper environment for optimal use
• Install your product indoors on a stable, vibration-free and level surface in order to prevent this instrument to fall thereby harming the operator
• Do not place the product in direct sunlight
• The ambient temperature should be between 5 to +40″C and humidity is maximum 80% at 31 degrees decreasing linearly to 50% at 40 degrees. Although the system is anti-mold treated, installing this product in a hot, humid location may still result in the formation of mold or condensation on lenses, impairing performance or causing malfunctions
• Never turn the right and left focus knobs in opposite directions at the same time or turn the coarse focus knob past its farthest point as this will damage this product
• Never use undue force when turning the knobs
• Make sure that the microscope system can dissipate its heat (fire hazard)
• Keep the microscope approximately 15 cm free from walls and obstructions
• Never turn the microscope on when the dust cover is in place or when items are placed on the microscope
• Keep flammable fluids, fabrick, etc. well out of the way

Disconnect power
Always disconnect your microscope from power before doing any maintenance, cleaning, assembling or replacing LEDs to prevent electric shocks

Prevent contact with water and other fluids
Never allow water or other fluids to come in contact with your microscope, this can cause short circuiting your device, causing malfunction and damage of your system

Moving and assembling

• This microscope is a relatively heavy system, consider this when moving and installing the system
• Always lift the microscope by holding the main body and base of the microscope
• Never lift or move the microscope by its focusing knobs, stage or head
• When needed, move the microscope with two persons instead of one

Models

The iScope microscope is available in the following brightfield and phase contrast models. Please note: On www. euromex.com you can find the latest updates about iScope models and accessories
I Scope• for Life Science (finity models) medical device class I

Objectives

(2)
Plan PU IOS| 4x/0.10| l0x/0.25| 20x/0.40 ( 1)| S40x/0.65| S60x/0.85(1)| Sl00x/1.25 oil (2)
Plan phase PLPH| | l0x/0.25| 20x/0.40| S40x/0.65| | Sl00x/1.25 oil (2)
Plan phase PLPHi IOS| | l0x/0.25| 20x/0.40| S40x/0.65| | Sl00x/1.25 oil (2)

(1) optional objectives (2) oil immersion objectives The S40x, S60x and S100x objectives are equipped with a spring mount, to prevent damage to the front lens and the slide. The Numeric Aperture – N.A. – of the objective is an indication for the resolving power of the objective  The total magnification can be calculated by multiplying the magnification of the eyepiece with the magnification of the objective. The magnifications are displayed in the table below:

14′.1.!,t   •

10x

| …

4x

|

40x

| .
---|---|---|---
10x| 10x| 100x
10x

10x

| 20x

40x

| 200x

400x

10x

10x

| 60x

100x

| 600x

1000x

Components of the microscope

The names of the several parts are listed below and are indicated in the picture:

Preparing the iScope microscope for use

Carefully remove the items from their packing and place them on a flat, firm surface. Please do not expose the microscope to direct sun light, high temperatures, damp, dust or acute shake. Please make sure the worktable is flat and horizontal When moving the microscope, use the left hand to hold the transport handle (A} at the back of the microscope and with the right hand the bottom of the microscope Caution! Holding the microscope by the stage, thestage focusing knob will damage the microscope Insert the power cord into the back of the microscope and use the CSS – Cable Storage System -to store excessive cable while in use or the entire cable after use (B}

Insert the power cord into the back of the microscope and use the CSS – Cable Storage System -to store excessive cable while in use or the entire cable after use (B}
Caution! If the bacterial solution or water splatters over the stage, objective or head, pull out the power cord immediately and dry the microscope
For safety reasons, make sure the power switch is turned off, the plug removed before replacing the led unit or fuse
Assembling steps If the bacterial solution or water splatters over the stage, objective or head, pull out the power cord immediately and dry the microscope
For safety reasons, make sure the power switch is turned off, the plug removed before replacing the led unit or fuse

Assembling steps

Euromex Microscopes will always try to keep the number of assembly steps for their customers as low as possible but in some cases there are some steps to be taken. The steps mentioned below are often not necessary but described for your convenience nonetheless

Mounting the objectives

• Rotate the coarse focusing knob to lower the stage to the lowest position
• Install the objectives into the objective nosepiece from the lowest magnification to the highest in a clockwise direction from the rear of the microscope. When using the microscope, start by using the low magnification objective (4X or lOXJ to search for specimen and focus, and then continue with a higher magnification objective to observe

The microscope head
To assemble the iScope head, please follow these steps:

• Remove the black cover from the upper side of the stand as indicated in picture A (next page}, using the Allen wrench supplied with the microscope
• Remove the transparent cover on the bottom of the head (picture B}
• Place the head of the iScope on the stand and fix it with the Allen wrench as indicated in picture C. The dovetail on the bottom fits into the slot on the top of the body

Placing the eye pieces

• Remove the cover of eyepiece tube
• Insert the eyepiece into the eyepiece tube (picture D}

Locking the eyepieces on iScope
For models without diopter adjustment, please find the screw for locking the eyepiece on the tube ring (picture E}. Please note that location can be slightly rotated from model to model. For models with diopter adjustment, take out the eyepiece (picture F} and look into the tube to find the right position of screw (picture G}

The eyeshades (optional)
Each eyepiece has a rubber eyeshade. This prevents damage to the lens, and stray light. The eyeshade can simply be slipped over the eyepiece

Connecting the power cord
The iScope series microscopes support a wide range of operating voltages: 100 to 240V. Please use a grounded power connection

• Make sure the power switch is off before connecting
• Insert the connector of power cord into the iScope power socket, and make sure it connects well
• Insert the other connector into the mains socket, and make sure it connects well.

Do not bend ortwlstthe power cord, it will get damaged. Use the special cord supplied by Euromex. If it’s lost or damaged, choose one with the same specifications

Operation:

Setting up the illumination
For optimal effect in contrast and resolution one should follow the below procedure:

• Place a specimen on the object stage and focus using the 4x objective with a fully opened iris diaphragm
• Turn light intensity to the lowest position, then look through the eyepiece(s) and turn up to a comfortable intensity level
• Turn the condenser in the highest position (for phase-contrast models, please set the condenser to brightfield position)
• Close the iris diaphragm, until it is just visible on the edge of the field of view

The microscope is properly set for use with the 4x objective. For each other magnification in brightfield use this procedure should be repeated to ensure the best balance between contrast and resolution. Phase contrast use will be explained further on in this manual  The maximum light intensity when using the 4x and lOx can damage the eyes!
Placing the specimen slide

• Push the arm of the specimen holder backward
• Release the arm slowly clamping the slide with the cover glass facing up
• Rotating the X and Y-axis knob will move the specimen to the center for alignment with the center of the objective

Focusing and slide protection

• Select the objective 4x to the optical path
• Observe the right eyepiece with right eye. Rotate the coarse focusing knob until the image appears
• Rotate the fine focusing knob for detailed focusing
• When focused with Sl00x objective, lock the slide protection handle. The slide protection handle protects the slide by limiting the travel of the table. This way the objective will not touch and damage your slides

Adjusting the focusing tension
The iScope microscope focusing knobs can be adjusted for tension. You can set it from light to heavy according to your own preference. Please note that when the specimen leaves the focus plane after focusing or the stage declines by itself, the tension should be set higher. To tighten the focusing arm (heavier), rotate the tension adjustment ring according to the arrowhead pointed; to loosen it, please turn it in the reverse direction

eyepieces
Using a binocular (or trinocular) tube is less tiring for the eyes than the use of a monocular tube. In order to obtain a smooth “compound’ image, one should go through the steps below

The interpupillary distance
The correct interpupillary distance is reached when one round image is seen in the field of view (see image below). This distance can be set by either pulling the tubes towards each other or pulling them from each other. This distance is different for each observer and thus should be set individually. When more users are working with the microscope it is recommended to remember your interpupillary distance for a quick set-up during new microscopy sessions. The iScope’s swiveling eyepiece tube can be rotated 360°. You can select corresponding eye point height according to your own preference

The correct eye point
The eye point is the distance from the eyepiece to the user’s pupil. To obtain the correct eye point, move the eyes towards the eyepieces until a sharp image is reached at a full field of view

Adjusting the diopter(s)
In order to compensate for human eye differences, distortion, thickness differences in cover glasses and tune for the best parfocality between objectives, one can use the diopter to do so. Take a good prepared slide for your reference:

Microscope models equipped with one diopter adjustment

• Position the diopter marking on the zero point
• Look into both eyepieces and focus on the specimen
• Close the right eye and look into the eyepiece with diopter adjustment, rotate the diopter adjustment from•+• to•-• until! the selected area get as sharp as possible

Microscope models equipped with two diopter adjustments

• Set (both) the diopter adjustments of the eyepieces to ·o•
• Select the l0x objective, look for a interesting area on the specimen and focus on this area
• Select the 40x objective and focus on the specimen

Warning: don’t change the coarse and fine adjustment anymore

• With your dominant eye open (close your other eye), rotate the diopter adjustment from•+• to• -• untill the selected area get as sharp as possible as with the 40x objective
• If during this operation the image becomes unsharp, take your eyes from the eyepieces and turn the diopter adjustment, without looking into the eyepieces, a few divisions back from ·-·to•+•
• Look into the eyepieces again and turn the diopter adjustment from ‘+’ to ‘-‘ until! the selected area on your specimen gets the optimal sharpness
• Repeat for your non-dominant eye, and with the second diopter

Verification:

• Take your eyes from the eyepieces and look for 2 seconds to a far point in the room in order to •reset• your eyes
• Look again into the eyepieces. If the adjustment is not good, repeat the operation till you reach the same sharpness for the l0x and 40x objective without touching the coarse and micrometric adjustments

Abbe condenser
Beneath the object stage an Abbe condenser N.A. 12.5 is mounted. The condenser can be adjusted in height by means of a rack and pinion movement and knob. With this, one can focus the light on the specimen by which the contrast can be optimized. The condenser is factory pre-centered. If needed the following procedure can be followed to center the condenser.

1. Move the condenser to the highest position
2. Select the 10x objective into the light path and focus the specimen
3. Rotate the field diaphragm adjustment ring to put the field diaphragm to the smallest position
4. Rotate the condenser up/down knob, and adjust the image to be clearest
5. Adjust the center adjustment screw and put the image to the center of the field of view
6. Open the field diaphragm gradually. If the image is in the center all the time and inscribed to the field of view, the condenser has been centered correctly

The field (Kohler) diaphragm (Al
By limiting the diameter of the beam entering the condenser, the field diaphragm can prevent stray light and enhance the image contrast When the image is just on the edge of the field of view, the objective can show the best performance and obtain the clearest image. The diaphragm is factory pre- centered

Adjusting the Aperture Diaphragm (Bl

• The diaphragm is used to adjust the Numerical Aperture of the condenser. When the N.A. of the condenser matches the N.A. of the objective, the highest possible resolution is obtained
• When contrast is low, rotate the diaphragm adjustment ring to 70%-80% of the N.A. of objective. This will improve the contrast of the image. The diaphragm is factory pre-centered

Use of the S1 00x oil-immersion objective
The Euromex iScope microscopes are equipped with an S100x N.A. 1.25 oil immersion objective. Please follow these instructions for using this objective:

1. Remove the dust protection from the revolving nosepiece to mount the S100x objective
2. Focus the image with the S40x objective
3. Turn the revolving nosepiece so the S100x objective almost reaches the click-stop
4. Put a small drop of immersion oil on the center of the slide (always use Euromex Immersion oil)
5. Now turn the S100x objective so that you feel the click stop
6. The front lens is in contact with the immersion oil
7. Look through the eyepiece and focus the image with the fine adjustment knobs
8. The distance between the lens of the objective and the slide is very small!
9. In case there are small bubbles visible turn the S100x objective a couple of times left/right so that the front of the objective moves in the oil and the bubbles will disappear
10. After using the S100x objective turn the table with the fine adjustment knobs downwards until the front lens
11. Always clean the front lens of the S 100x objective with a piece of lens paper that is moistened with a drop of isopropanol. We recommend using Euromex lens paper isopropanol
12. Clean the slide after use as well

Safety device
To prevent damage to the objective lens or the slide, all types are equipped with a pre-fixed safety device. It is recommended to use slides of 1.0- 1.2 mm thickness (product numbers: PB.5150, PB.5155, PB.5160) in combination with cover glasses of 0.13 mm or 0.17 mm thickness (product numbers: PB.5165, PB.5168)

Illumination of the iScope
The illumination has the following specifications:

• LED : 3W NeoLED for binocular and trinocular models
• Power supply : Primary AC 100 – 240 Volt-SO Hz

”iCare” Function
When the operator leaves the microscope, after 20-30 minutes the light source will be turned off automatically. The indicator(1) will flash once every 3 seconds. On return, press iCare function button(2), which will turn the light back on again. To turn off the iCare function press the button for 3 seconds. This will cause the red indicator led(1) to turn off and the microscope light is always on. Press the button for another 3 seconds, it will make indicator flash and the iCare function is back on

Phase-contrast

Use of phase contrast with the iScope microscope
The phase-contrast method was designed in 1934 by Dutchman Frits Zernike to observe very thin or transparent objects. This technique uses the fact that light traveling through tissue undergoes a phase shift due to diffraction
By recombining the phase-shifted light with the background light, a contrasted image appears in the eyepiece

Using the Phase Contrast Slider

• Keep the phase contrast slider face up (text up); insert it from left to right into the condenser slider socket as the direction of the arrow pointed
• Each slider has 3 positions, 2 phase contrast positions and in the center of the slide the brightfield position for normal use without phase contrast. Each phase contrast objective used has to be matched with the phase contrast ring on the slider. For example: when the 10x phase contrast objective is used the slider should be positioned to match the 10 phase diaphragm)

Note: the phase diaphragms in the sliders are pre-centered and do not need to be adjusted in operation
Using the Zernike phase contrast set
Any iScope model with a Zernike phase contrast set comes with the condenser and objectives already mounted and centered on your microscope. If you suspect misalignment or want to check the alignment please see the next point for “centering the phase rings” The height of condenser can be adjusted in height by means of a rack and pinion movement. In this way the light beam is concentrated in the specimen for an optimum resolution

Centering the phase rings
The Zernike phase disc has five positions:

• “DF’ for darkfield observation (up to 400x),
• “BF’ for brightfield observation, this position also has an iris diaphragm
• And “10/20”, •40• •100- which are corresponding to phase contrast observation using 10x, 20x, 40x, 100x objectives respectively

When the condenser is in the DF or BF position the objectives can be used for either darkfield or bright field. For phase contrast, the condenser position should match the objective used. Meaning that when the condenser is in position “40” the objective used should also be 40x

• Rotate 10x infinity plan phase contrast objective into the field of view, then set the condenser to match the objective (marker “10/20′)
• Take the eyepiece out of the tube and insert the centering telescope in its place. Observed from centering telescope, the dark and bright ring images should coincide with each other as shown in the figures below. If the ring images can’t be observed clearly, first try and focus the centering telescope. If this does not solve the issue raise or decline the condenser
• If the bright ring and dark ring images are not coincided as shown below, adjust the position of the ring with the two screw keys on the side of the condenser to move the ring until bright and dark ring images superimpose. Repeat for all objectives/Zernike disc positions

Maintenance and cleaning

Always place the dustcover over your iScope microscope after use. Keep the eyepieces and objectives always mounted on the microscope to avoid dust entering the instrument

Cleaning the optics
When the eyepiece lens or front lens of the 10x or S40x objective are dirty they can be cleaned by wiping a piece of lens paper over the surface (circular movements). When this does not help put a drop of alcohol on the lens paper. Never put isopropanol or alcohol directly on the lens! Please note that Euromex offers a special microscope cleaning kit PB.5275 It is not necessary -and not recommended -to clean the lens surfaces at the inner side of the objectives. Sometimes dust can be removed with high pressured air. There will never be dust in the objectives if the objectives are not removed from the revolving nosepiece

Maintenance of the stand
Dust can be removed with a brush. In case the stand or table is really dirty the surface can be cleaned with a non-aggressive cleaning product All moving parts – like the height adjustment or the coaxial course and fine adjustment – contain ball bearings that are not dust sensitive. With a drop of sewing machine oil the bearing can be lubricated

Replacing the fuse
To change the fuse, follow the procedure below:

• Unplug the system from power and place the microscope with back toward you
• Find the fuse cover that will appear as a round protrusion with a slot
• Use a small flathead screwdriver or another flat object (coin, etc) to gently push the fuse cover in and turn the cover counterclockwise. You need to turn the cover about 3/4 of a turn
• The fuse cover will pop out with the fuse attached
• Remove the fuse from the cover and examine the fuse. If the thin piece of metal going from one end of the fuse to the other has a gap, then the fuse is bad
• If the fuse is bad, install a replacement fuse in the cover
• Gently push the fuse cover with the new fuse back into the body until it is flush with the unit. Turn the cover clockwise about 3/4 to secure the cover back into the unit

Note: Fuse may blow in order to protect internal damage to the microscope. And in most cases, replacing the fuse with the correct voltage will resolve the issue. However, should you encounter a blown fuse frequently, please contact your distributor for further assistance Fuse specification: 250v S00mA

Digital cameras

Digital cameras are designed to be used on the photo port of the microscope head (fig. 1). It is also possible to use the digital camera in combination with a binocular head (fig.2). For infinity type iScopes, simply remove the eyepiece[!] and place the 30mm adapter ring into the eyepiece tube [2] then place the camera with mounted c-mount adapter in the eyepiece tube [3]. Focus the digital image with the coarse and fine controls of the microscope. For finite(160mm) iScopes the procedure is the same but there is no need to use an adapter [2] For trinocular models, slide the camera with mounted c-mount adapter into the 23.2 mm tube of the photo port. For focussing, slowly unscrew the tube (A) you will be able to match parfocality of the camera with the view through the eyepieces by moving the camera up and down inside the 23.2 mm tube. Take an easy-to-view specimen and focus the image through the microscope’s eyepieces (with diopter adjustment set on ‘O”). Afterwards, perform the height adjustment procedure above while watching the image on the computer screen. In this case, once you have obtained parfocality in the device, tighten screw (A) again. Screw (B) is only used to fix the 23.2mm tube on the iScope’s photo port. Follow the manual that comes with the camera for camera operation

iScope SLC (Smart Light Control)

The Smart Light Control features are all controlled with only one single button (A). Simply rotate the button to change light intensity and the SLC automatically stores this intensity for the selected objective. Push the button once to enter or exit sleep mode and push the button twice to enter or exit lock mode

Adding or changing an objective in the menu

The iScope SLC system is preconfigured at the factory. If users want to add or change objectives this can be done via the objective selection menu;

• To enter the objective selection menu, push the control button (and keep pushing) while switching on the microscope. The menu will now appear
• Push the button once to switch between revolver positions. Rotate the button to change the magnification of the selected revolver position

Accessories and spare parts

For current accessories and spares, please visit websitewww.euromex.com

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