IDEXX Urine Sediment SediVue Dx Analyser User Guide
- June 13, 2024
- IDEXX
Table of Contents
IDEXX Urine Sediment SediVue Dx Analyser User Guide
Blood cells
Figure 1. Red blood cells
Figure 2. Crenated red blood cells
Figure 3. White blood cells
Figure 4. White blood cells
Epithelial cells
Figure 5. Squamous epithelial cells
Figure 6. Squamous epithelial cells
Figure 7. Numerous transitional (nonsquamous) epithelial cells with RBCs
and WBCs
Figure 8. Numerous transitional (nonsquamous) epithelial cells (Possible
transitional cell carcinoma. Confirm with dry-slide cytology.)
Bacteria
Figure 9. Rods with white blood cells
Figure 10. Rods with white and red blood cells
Figure 11. Cocci with white blood cells
Figure 12. Cocci in chains
Casts
Figure 13. Left and right, hyaline cast
Figure 14. Cellular (nonhyaline) cast
Figure 15. Numerous granular (nonhyaline) casts
Figure 16. Waxy (nonhyaline) cast
Crystals
Figure 17. Large struvite crystals
Figure 18. Numerous small struvite crystals
Figure 19. Large calcium oxalate dihydrate crystals
Figure 20. Numerous calcium oxalate dihydrate crystals
Figure 21. Calcium oxalate monohydrate (picket fence) crystals
Figure 22. Calcium oxalate monohydrate crystals; left, dumbbells; right,
hemp seed
Figure 23. Ammonium biurate (thorn apple) crystals
Figure 24. Bilirubin crystal with WBCs
Figure 25. Cholesterol crystals
Figure 26. Cystine crystals with red blood cells
Figure 27. Uric acid crystals
Figure 28. Likely drug-related crystals
Miscellaneous
Figure 29. Lipids
Figure 30. Amorphous crystalline debris
Figure 31. Hyphae
Figure 32. Sperm with white blood cells
Figure 33. Left, Pearsonema spp. (Capillaria spp.) ova; right,
macrocanidia
Figure 34. Left, glove powder; right, pollen
Figure 35. Fiber
Figure 36. Dust mite
Conventional microscopy
All images, unless otherwise indicated, are representative of a high power (40x objective) field of view.
Blood cells
Figure 1. Erythrocytes and one squamous epithelial cell
Figure 2. Erythrocytes and two leukocytes (black arrows
Figure 3. Numerous leukocytes and few rod-shaped bacteria
Epithelial cells
Figure 4. Squamous epithelial cells
Figure 5. Epithelial cells (black arrows), RBC (red arrows) and WBC (blue
arrows)
Figure 6. Transitional epithelial cells
Figure 7. Left, Transitional cell carcinoma; right, NMB wet prep
Figure 8. Transitional cell carcinoma (NMB wet prep on right)
Figure 9. Transitional cell carcinoma, air-dried and Diff-Quik* stained
Bacteria
Figure 10. Many rod-shaped bacteria,100× objective field of view
Figure 11. Many leukocytes and large rod-shaped bacteria (black arrows
Figure 12. Numerous bacteria and leukocytes
Casts
Figure 13. Hyaline cast (borders outlined)
Figure 14 Left, granular cast; right, mixed waxy and granular cast
Figure 15. Waxy cast
Crystals
Figure 16. Struvite
Figure 17. Amorphous (NMB wet prep on right)
Figure 18. Bilirubin
Figure 19. Ammonium biurate
Figure 20. Left, calcium oxalate monohydrate; right, calcium oxalate
dihydrate
Figure 21. Drug (Tribrissen*) crystals, 10× objective field of vi
Conventional images and information provided by Dennis B. DeNicola, DVM, PhD, DACVP; Rick L. Cowell, DVM, MS, MRCVS, DACVP; and Graham Bilbrough, MA, VetMB, CertVA, MRCVS.
How to perform a dry prep/line smear
Performing a dry prep or line smear is an extremely cost-effective means of confirming the presence or absence of bacteria, of differentiating between cocci and short rods, and for characterizing various cellular elements in the urine sample.
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Label your slides appropriately.
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Fill a centrifuge tube with well-mixed, fresh urine taken from the bottom of the sample tube.
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Centrifuge the sample (and a balance tube) on the Urine setting (or 400 g).
Note: If your centrifuge does not have a Urine setting, refer to its operator’s manual for centrifugation settings and times. -
After centrifugation, a concentrated pellet of formed elements should be visible at the bottom of the tube. Gently aspirate the supernatant down to the pellet, leaving an extremely small amount of urine to resuspend the pellet.
Note: If the sample is extremely hypocellular, it may be very difficult to see the pellet. -
Lightly flick the bottom of the tube multiple times with your finger to gently resuspend the formed elements.
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Using a new pipette, dispense a drop of sample on a glass slide, similar to preparing a blood film.
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Place a clean glass spreader slide on your labeled slide, at approximately 30°–40°, in front of the drop of urine.
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Back the spreader slide into the drop allowing the material to spread along the edge of the spreader slide.
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Move the spreader slide toward the end of the specimen slide, keeping the two in contact with each other.
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In the middle of the slide, abruptly stop spreading the urine sample and lift the spreader slide straight up to form a line of material.
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Air dry thoroughly and then stain the slide using your routine hematology/cytology stain (e.g., Diff-Quik*).
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Review microscopically.
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*SediVue Dx is a trademark or registered trademarks of IDEXX Laboratories, Inc. or its affiliates in the United States and/or other countries. All other product and company names and logos are trademarks of their respective holders.
