IDEXX SediVue Dx Urine Sediment Analyzer Instructions

IDEXX SediVue Dx Urine Sediment Analyzer

Your key to understanding urinalysis dilutions

• When a urine sample is crowded and the edges of the elements overlap, the SediVue Dx convolutional neural network may have difficulty discerning the elements from one another. Diluting the urine sample will help spread the elements apart for easier identification and classification.
• This document will help you identify when dilutions may be necessary both preanalysis and postanalysis and provide you with insight to help reduce additional dilution work-flow steps.
• Note: Diluting a urine sample may affect the pH and osmolality of the sample and lead to changes in cellular appearance and the presence of crystals.

Preanalysis dilutions guide

• There are times during the physical evaluation of the clarity and color of urine samples when it’s obvious that samples will be crowded with cells, bacteria, debris, or crystals (e.g., gross hematuria). This evaluation will aid in determining if a dilution should be considered preanalysis.
• Use the guidelines below to determine if the sample should be diluted prior to running it on the SediVue Dx analyzer.
• Note: It’s always good practice to remove an aliquot of the original sample in case additional testing is needed.

Postanalysis dilutions guide

• When a “crowded” sample is run on the SediVue Dx analyzer, your semiquantitative results may be suppressed and you will be prompted to consider a dilution. When this occurs, an image review is essential to determine the appropriate next steps.
• If the images provide clinical insight, a simple annotation to the patient record may be applied and you may be able to move on without a dilution.1
• If the images do not provide clinical insight, diluting the urine sample will help to spread the elements apart for more accurate analysis. The dilution ratio will vary depending on the severity of the crowding in the sample. Follow the guidelines below to determine if a postanalysis dilution may be helpful.

Performing a dilution†

1. On the Select Instruments screen, tap Run Dilution, specify the desired dilution factor (total parts), and tap Run.
2. In a test tube, mix the urine with the selected parts of 0.9% normal saline 10 times.
3. Immediately inject 165 μL of the diluted sample into the cartridge fill port.
4. Press the Start button on theanalyzer. †IDEXX VetLab* Station version 4.48 or higher required.

Do the images provide clinical insight?
If you are prompted to consider a dilution but you can obtain clinical insight from the images, a dilution is unnecessary.2

Minimal crowding

Extreme crowding

1. Dilution flags may also result from excessive bubbles and/or fibers from dirty optics.
2. Only the first run for each sample type for the same patient in a 24-hour period will be invoiced. Multiple runs from the same patient that include one or more dilutions and do not provide semiquantitative results will not be invoiced.
3. It may be helpful to confirm bacteria with an air-dried, stained preparation (“dry prep”), rather than a dilution.
4. Higher concentrations of diluent will affect the pH and osmolality of the sample and lead to changes in cellular appearance and the presence of crystals.

© 2017 IDEXX Laboratories, Inc. All rights reserved. • 06-0007664-02 *SediVue Dx and IDEXX VetLab are trademarks or registered trademarks of IDEXX Laboratories, Inc. or its affiliates in the United States and/or other countries.

Have questions?

• Contact IDEXX Technical Support:
• United States/Canada: 1-800-248-2483
• Europe: idexx.eu
• Distributors:
• [email protected]
• Ireland: 353 (0)1 562 1211
• United Kingdom: 44 (0)20 3788 7508
• Australia: 1300 44 33 99
• New Zealand: 0800 10 20 84

References

• Home - IDEXX UK
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