dynamic BIOSENSORS DC-0 v5.1 Density Control Kit User Manual
- August 27, 2024
- dynamic BIOSENSORS
Table of Contents
dynamic BIOSENSORS DC-0 v5.1 Density Control Kit
Key Features
- Complementary Anchor strand 1 and complementary Anchor strand 2 for low density generation of heliX ®
- Adapter Chip Spot 1 and Spot 2.
- Compatible with heliX ® Adapter Chip.
- Ideal for MIX RUN sample preparation.
- This kit enables to modulate the ligand density on the sensor surface.
® Adapter Chip Overview
2 spots with 2 different anchor sequences for DNA-encoded addressing.
Product Description
Order Number: DC-0
Table 1. Contents and Storage Information
Material| Cap| Concentration| Amount| Buffer|
Storage
---|---|---|---|---|---
cAnchor strand 1| Red| 200 nM| 5 x 200 µL| TE40 [1]| -20°C
cAnchor strand 2| Red| 200 nM| 5 x 200 µL| TE40 [1]| -20°C
For research use only.
This product has a limited shelf life, please see expiry date on label.
To avoid many freeze thaw cycles please aliquot the nanolayer.
Preparation | MIX RUN
In-solution hybridization of adapter and ligand strands:
- Mix Adapter strand 1 – Ra (400 nM) and conjugated Ligand strand (500 nM) at 1:1 ratio (v/v).
- Incubate the solution of step 1 at RT at 600 rpm for 30 min to ensure complete hybridization.
- Mix solution of step 2 and Adapter strand 2 – Ra – lfs (200 nM) at 1:1 ratio (v/v). Solution is ready to use for biochip functionalization Stability of the solution is related to the stability of the ligand mole
Preparation | Ligand Density Variation
Easy modulation of ligand density on the sensor surface:
- Mix cantor strand 1 and Anchor strand 2 at 1:1 ratio (v/v).
- Mix solution of step 3 and solution of step 4 at the ratio of desired on-chip density, e.g.:
- a. 1:1 ratio (v/v) for a 50% ligand density
- b. 1:2 ratio (v/v) for a 30% ligand density
- c. 1:9 ratio (v/v) for a 10% ligand density
Solution is ready to use for biochip functionalization
Example
Required volume for 3 functionalizations: 100 µL with a final ligand density of 10 %.
| Vial 4 | Vial 4 |
|---|
Adapter strand 1 – Ra with ligand (100 nM) Adapter strand 2 – Ra – lfs (100
nM)| cAnchor strand 1 (200| cAnchor strand 2 (200 nM
10 µL| 45 µ| 45 µL
Mix vial 3 and vial 4 to obtain 100 µL of ready-to-use DNA solution.
Useful Order Numbers
| Product | Comment | Order No |
|---|---|---|
| heliX ® Adapter Chip | Chip with 2 detection spots | ADP-48-2-0 |
| heliX ® Amine Coup | For five individual conjugation reactio | HK-NHS-1 |
| Adapter strand 1 – R | 400 nM | AS-1-Ra |
| Adapter strand 2 – | 200 nM, prehybridized with Ligand-free stran | AS-2-Ra |
Customer Support
Contact
Dynamic Biosensors GmbH
Perchtinger Str. 8/10
81379 Munich
Germany
Dynamic Biosensors, Inc.
300 Trade Center, Suite 1400
Woburn, MA 01801
USA
Order Information order@dynamic-biosensors.com
Technical Support support@dynamic-biosensors.com
www.dynamic-biosensors.com
Instruments and chips are engineered and manufactured in Germany.
©2024 Dynamic Biosensors GmbH | Dynamic Biosensors, Inc. All rights reserved.
[1] TE40: 10 mM Tris, 40 mM NaCl, 0.05 % Tween20, 50 μM EDTA, 50 μM EGTA
www.dynamic-biosensors.co
References
Read User Manual Online (PDF format)
Read User Manual Online (PDF format) >>