3B Scientific ME5 Monocular Microscope Instruction Manual

3B Scientific ME5 Monocular Microscope

Specifications

Features and Components

• All metal stand with arm permanently connected to the base
• Separate knobs for coarse and fine adjustment
• Wide field plan eyepiece PL 10x 18 mm
• Inverted objective revolver with 4 achromatic objectives
• x-y mechanical stage with object guide and coaxial adjustment knobs
• Adjustable LED lighting integrated in base
• Abbe condenser N.A.1.25 with iris diaphragm and filter holder

Dimensions and Weight Dimensions: 350 mm x 213 mm x 366 mm approx.

Weight: 8 kg approx.

Unpacking and Assembly

1. Remove the microscope from the styrofoam container carefully.
2. Install the objectives into the nosepiece in a clockwise direction.
3. Insert and secure the eyepiece into the tube.
4. Secure the head using the provided hex keys.

Operation General Information

1. Place the microscope on a level table.
2. Position the object at the center of the object plate.
3. Connect the mains cable and turn on the switch for illumination.
4. Ensure the specimen is centered over the stage opening.

Eyepiece Dioptre Adjustment To adjust the eyepiece dioptre:

1. Look through one eyepiece with your dominant eye.
2. Cover the other eyepiece with your hand.
3. Rotate the dioptre adjustment ring on the eyepiece until the image is sharp.
4. Repeat for the other eyepiece if needed.

FAQ

Q: How do I clean the lenses of the microscope? A: To clean the lenses, use a soft, lint-free cloth or lens paper. Avoid using harsh chemicals or solvents.

Q: Can I use this microscope for viewing live specimens? A: Yes, you can view live specimens with this microscope. However, ensure proper handling and preparation of the specimens to maintain clarity and focus.

Description, technical data

The microscopes E5 allow two-dimensional viewing of objects (thin sections of plant or animal speci-men) in 40x to 1000x magnification.
Besides the binocular eyepiece, the microscope model TE5 also offers the option of connecting a camera for photographic and/or video documentation.

1. Eyepiece
2. Tube
3. Revolving nosepiece with objectives
4. Object stage with object guide
5. Condenser with iris diaphragm and filter holder
6. Illumination
7. Mains switch
8. Head
9. Stand
10.  x-y Cross-table adjustment
11. Retaining lever
12. Fine adjustment
13. Coarse and fine adjustment

Stand

| Robust, all metal stand with arm permanently connected to the base. Fo- cussing by means of separate knobs for coarse and fine adjustment located on either side of the stand and operated by rack and pinion drive with ball bearings and retaining lever, adjustable stopper for protecting the object slides and objective.

Resolution of fine focussing adjustment: 0.002 mm

Tube| Monocular inclined 30°,

head rotation 360°

| Monocular inclined 30°,

head rotation 360°

| Monocular inclined 30°,

head rotation 360°

Eyepieces| Wide field plan eye- piece PL 10x 18 mm| Wide field plan eyepiece PL 10x 18 mm
Objectives| Inverted objective revolver with 4 achromatic objectives 4x / 0.10, 10x / 0.25, 40x / 0.65, 100x / 1.25 (oil)
Enlargement| 40x, 100x, 400x, 1000x

Object Stage

| x-y mechanical stage, 132 mm x 140 mm, with object guide and coaxial adjustment knobs perpendicular to the object stage, adjustment range 76 mm x 50 mm, accuracy 0.1 mm
Illumination| Adjustable LED lighting integrated in base, universal 100 to 240 V, 50/60 Hz power supply
Condenser| Abbe condenser N.A.1.25 with iris diaphragm , filter holder
Dimensions| 350 mm x 213 mm x 366 mm approx.
Weight| 8 kg approx.

Unpacking and assembly

1. The microscope is packed in a molded styrofoam container.
   • Take the container out of the carton remove the tape and carefully lift the top half off the container. Be careful not to let the optical items (objectives and eyepieces) drop down.
   • To avoid condensation on the optical com-ponents, leave the microscope in the original packing to allow it to adjust to room temper-ature.
   • Using both hands (one around the pillar and one around the base), lift the microscope from the container and put it on a stable desk.
   • The objectives will be found within individual protective vials. Install the objectives into the microscope nosepiece from the lowest mag-nification to the highest, in a clockwise direc-tion from the rear.
   • Insert the eyepiece into the tube and secure it in place using the small hex key.
   • Secure the head with the large hex key.

Operation

General information

• Set the microscope on a level table.
• Place the object to be observed in the center of the object plate.
• Connect the mains cable to the net and turn on the switch to get the object illuminated.
• Make certain that the specimen is centered over the opening in the stage.
• Make the necessary eyepiece dioptre ad-justments to suit your eyes (refer to 3.2).
• Adjust the interpupillary distance so that one circle of light can be seen (refer to 3.3).
• Adjust the aperture of the iris diaphragm to get the background brightness suitable for a high contrast image.
• Rotate the nosepiece until the objective with the lowest magnification is pointed at the specimen. There is a definite “click” when each objective is lined up properly.
  NOTE: It is best to begin with the lowest power objective. This is important to reveal general structural details with the largest field of view first. Than you may increase the magnification as needed to reveal small details.

To determine the magnification at which you are viewing a specimen, multiply the power of the eyepiece by the power of the objective.

• Adjust the coarse-focusing-knob which moves the stage up until the specimen is fo-cused. Be careful that the objective does not make contact with the slide at any time. This may cause damage to the objective and/or crack your slide.
• Adjust the fine-focusing-knob to get the im-age more sharp and more clear.
• Colour filters may be inserted into the filter holder for definition of specimen parts. Swing the filter holder out and insert colour filters.
• Be careful not to spill any liquids on the mi-croscope.
• Do not mishandle or impose unnecessary force on the microscope.
• Do not wipe the optics with your hands.
• Do not attempt to service the microscope yourself.

Dioptre adjustment (BE5 and TE5)

• Look through the right-hand eyepiece with your right eye and focus on the object.

• Then look through the left-hand eyepiece with your left eye. If the image is not well fo-cussed, turn the dioptre adjustment ring (1) until it is (see Fig. 1).
  The dioptre adjustment ring (1) is marked with dioptric values of ±5. The value to which the scale marking (2) points corresponds to the dioptric value for the user.

• Set up the same value next time you use the microscope as well.

Fig. 1 Dioptre adjustment

Setting of interpupillary distance (BE5 and TE5)

• Look through the eyepieces with both eyes and turn the tubes around the axle until you can see only one image field.
• Point (1) indicates the interpupillary distance of the user on the scale (2) (see Fig. 2).
• Set up the same interpupillary distance next time you use the microscope as well.

Adjustment of retaining attachment

 You need to adjust the retaining attachment
in order to fix the following problems: Dif-ficulty turning the coarse focus adjustment knob, slide slowly losing focus, sliding of ob-ject stage (see Fig. 3).

Turning the knob for the retaining attachment in the direction of the arrow secures the retaining attachment. Turning in the opposite direction loosens the focussing drive.

Changing the lamp

• Turn off the power switch and unplug the mains plug.
• Unscrew the base plate and remove it.
• Unplug the connector to the LED unit by pressing on the connector socket (1) and pulling out the plug (2) (see Fig. 3).
• Undo two screws (3), swap the faulty LED unit for a new one and then screw the mi-croscope back together.

Storage, cleaning, disposal

• Keep the microscope in a clean, dry and dust free place.

• When not in use always cover the micro-scope with the dust cover.

• Do not expose it to temperatures below 5°C and above 40°C and a max. relative humidi-ty of over 80%.

• Always unplug the mains plug before clean-ing or maintenance.

• Do not clean the unit with volatile solvents or abrasive cleaners.

• Do not disassemble objective or eyepieces to attempt to clean them.

• Use a soft linen cloth and some ethanol to clean the microscope.

• Use a soft lens tissue to clean the optics.

• Die Verpackung ist bei den örtlichen Recyc-lingstellen zu entsorgen.
  Should you need to dis-pose of the equipment itself, never throw it away in normal domestic waste. If being used in private households it can be dis-posed of at the local public waste disposal authority.

• Comply with the applicable regulations for the disposal of electrical equipment.

3B Scientific GmbH • Rudorffweg 8 • 21031 Hamburg • Germany ▪ www.3bscientific.com Subject to technical amendments
© Copyright 2016 3B Scientific GmbH

References

• Simulation and skills training for Medical Education - 3B Scientific

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