3B Scientific UE4020400 Digital Spectrometer LD with Absorption Chamber Instruction Manual

June 7, 2024
3B Scientific

3B Scientific UE4020400 Digital Spectrometer LD with Absorption Chamber

EXPERIMENT PROCEDURE

  • Measure and compare the transmission spectra of solid bodies.
  • Measure and compare the transmission spectra of liquids.

OBJECTIVE

  • Record and interpret transmission spectra of transparent bodies

SUMMARY

  • A digital spectrophotometer is used to measure transmission spectra.
  • In this instrument the transmitted light collected by an optical fibre is separated into its spectral components by a reflection grating using the Czerny-Turner principle and is projected as an image onto a CCD detector via two mirrors.
  • The transmission spectrum is generated by automatic normalisation applied to the previously recorded spectrum of the light falling on the detector.

REQUIRED APPARATUS

Quantity Description Item Number
1 1 Digital-Spectrometer LD with Absorption Module 1019196
1 Set of 7 Color Filters 1003084
1 Macro Cuvettes, 4 ml 1018106

Additionally recommended

  • Chlorophyll
  • Potassium Permanganate

BASIC PRINCIPLES

  • The observed colour of an object illuminated with white light depends on its reflecting properties. The perceived colour of light that has passed through an object depends on its light-transmitting properties.
  • For example, the perceived colour may be red if the object is transparent to red light while other colour components of the light are attenuated on passing through the object. In such a case spectral transmission is at a maximum for red light.
  • The unaided human eye cannot distinguish between a colour sensation caused by spectrally pure light and the same sensation caused by the addition of neighbouring colours of the spectrum.
  • Therefore, it is not possible to reach conclusions about the transmission spectrum solely from the observed colour. To determine it unambiguously is only possible with the help of a spectrometer.
  • In this experiment, transmission spectra are recorded using a digital spectrophotometer.
  • In this instrument, the transmitted light collected by an optical fibre is separated into its spectral components by a reflection grating using the Czerny-Turner principle and is projected as an image onto a CCD detector via two mirrors.
  • The transmission spectrum is generated by automatic normalisation applied to the previously recorded spectrum of the light falling on the detector.

EVALUATION

  • Spectral absorptivity A(λ) can be calculated directly from the spectral transmission coefficient T(λ) of a body if the effect of reflection at the surface is neglected. The relationship is: A(λ) = 1−T (λ)

3B-Scientific-UE4020400-Digital-Spectrometer-LD-with-Absorption-Chamber-
FIG-1

OPTICS | COLOUR | Transmission Spectra Transmission Spectra | COLOUR | OPTICS

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