ibidi 88407 Well Black Glass Bottom Instructions

ibidi 88407 Well Black Glass Bottom

Product Information

This product is a glass coverslip bottom for ibidi labware products. It is made of Schott borosilicate glass, D 263M with the following optical properties:

The glass coverslip or glass slide is fragile and can break easily, so it should be handled with care to avoid physical injury  and damage to devices through leakage of the medium.

Product Usage Instructions

To use this product, follow these steps:

1. Prepare your coating solution according to the manufacturer’s specifications or reference.
2. Apply the coating solution to the glass coverslip bottom.
3. Aspirate the solution and wash with the recommended protein dilution buffer.

Note: Detailed information about coatings for ibidi labware products can be found in Application Note 08: Coating protocols for ibidi labware products.

When using ibidi Glass Bottom products with oil immersion objectives, there is no known incompatibility with any immersion oil on the market. All types of immersion oils can be used.

For chemical compatibility, please refer to the following table:

This product is for research use only..

Instructions µ-Plate 384 Well Black Glass Bottom

The ibidi product family is comprised of a variety of µ-Slides, µ-Dishes, and µ-Plates which have all been designed for high-end microscopic analysis of fixed or living cells. The high optical quality of the material is similar to that of glass, so you can perform all kinds of fluorescence experiments with uncompromised resolution and choice of wavelength.
The µ-Plate 384 Well Black Glass Bottom allows you to perform high resolution microscopy in a standard multi–well format. For less well–to–well crosstalk in fluorescence microscopy this imaging plate is made out of a black polymer material.

Overview
This document is applicable to the following product numbers:

Cat. No. Product Name
88407: µ-Plate 384 Well Black Glass Bottom: #1.5H (170 µm ±5 µm) D 263 M Schott glass, sterilized, individually packed

Material
The µ-Plate 384 Well Black Glass Bottom is made with a glass coverslip bottom. It is not possible to detach the bot-tom. The µ-Plate 384 Well Black Glass Bottom is intended for one-time use and not autoclavable since it is tempera- ture stable only up to 80°C/175°F.

Optical Properties of the Glass Coverslip Bottom

• Refractive index nD 1.523
• Abbe number 55
• Thickness No. 1.5H (selected quality 170 μm, ± 5 μm)
• Material Schott borosilicate glass,D 263M

Attention!
Be cautious when handling ibidi labware products with glass bottom! The glass coverslip or glass slide is very fragile and might break easily. Handle with care to avoid physical injury and damage to devices through leakage of the medium.

Shipping and Storage
The µ-Slides, µ-Dishes and µ-Plates are sterilized and welded in a gas- permeable packaging. The shelf life under proper storage conditions (in a dry place, no direct sun-light) is listed in the following table.

Conditions

• Shipping conditions Ambient
• Storage conditions RT (15–25°C)

Shelf Life

• Glass Bottom 36 months

Geometry
The µ-Plate 384 Well Black Glass Bottom provides standard geometry and numbering (A-P, 1-24).
The µ-Plate 384 Well Black Glass Bottom meets all important values of the ANSI/SLAS (SBS) Standards (1-2004, 2-2004, 3-2004 and 4-2004).

Dimensions in mm

• Length 127.7 ± 0.3
• Width 85.5 ± 0.3
• Height with lid 17.2 ± 0.4
• Height without lid 15.0 ± 0.3
• Well to well distance 4.5 ± 0.1
• Well clearance 0.73 ± 0.1
• Focal offset 0.9 ± 0.1

Single Well Dimensions

• Single well dimensions 3.4 × 3.4 ± 0.1 mm
• Single well depth 12.8 ± 0.2 mm
• Volume 50 μl
• Growth area 0.11 cm2
• Coating area using 50 μl 0.8 cm2

Tip:
The volume of 50 µl is our recommended volume with sufficient culture medium for living cells and without fearing well-to-well crosstalk. Depending on your ap-plication, volumes between 20 µl and 100 µl may be used.

Surface
The µ-Plate 384 Well Black Glass Bottom is manufactured with an uncoated glass coverslip. Washing steps (e.g. with PBS) before cell seeding can remove glass dust which is advantageous for direct cell growth on the surface.

Coating
Detailed information about coatings is provided in Application Note 08: Coating protocols for ibidi labware products.

In short, specific coatings are possible following this protocol:

1. Prepare your coating solution according to the manufacturer’s specifications or reference.
2. Apply 50 µl and leave at room temperature for at least 30 minutes.
3. Aspirate the solution and wash with the recommended protein dilution buffer.
4. The µ-Plate 384 Well Black Glass Bottom is ready to be used. Optionally let dry at room temperature. Attention, some coating proteins might degenerate when drying!

Seeding Cells

• Trypsinize and count cells as usual. Dilute the cell suspension to the desired concentration. De-pending on your cell type, application of a 1.5–4 × 104 cells/ml suspension should result in a confluent layer within 2–3 days.
• Apply 50 µl cell suspension into each well. Avoid shaking as this will result in inhomogeneous distribution of the cells.
• Cover the slide with the supplied lid. Incubate at 37°C and 5 % CO2 as usual.

Undemanding cells can be left in their seeding medium for up to three days and grow to confluence there. How-ever, best results might be achieved when the medium is changed every 1–2 days. Carefully aspirate the old medium and replace it by 50 µl fresh medium per well.

Tip:
You can stack the µ-Plates to save space in your incubator. This will not affect cell growth. We recommend making batches with not more than 6 plates, due to sta-bility reasons.

Immersion Oil
When using ibidi Glass Bottom products with oil immersion objectives, there is no known incompatibility with any immersion oil on the market. All types of immersion oils can be used.

Chemical Compatibility
The following table provides some basic information on the chemical and solvent compatibility of the µ-Plate 384 Well Black Glass Bottom. For a full list of compatible solvents and more information on chemical compatibility, please visit the FAQ section on ibidi.com.

Chemical / Solvent Compatibility

• Methanol yes
• Ethanol yes
• Formaldehyde yes
• Acetone no
• Mineral oil yes
• Silicone oil yes
• Immersion oil See Immersion Oil on page 2.

Microscopy
To analyze your cells, no special preparations are necessary. Cells can be directly observed live or fixed, preferably on an inverted microscope. The bottom can-not be removed. For optimal results in fluorescence microscopy and storage of fixed and stained samples, ibidi provides mounting media (50001 and 50011) optimized for µ-Dishes, µ-Slides, and µ-Plates.

Ordering Information

The ibidi µ-Plates are available in the following product versions.

µ-Plate 24 Well Black ID 14 mm

Cat. No. Description

• 82426
  μ-Plate 24Well Black ID 14mmibiTreat: #1.5 polymer coverslip, tissue culture treated, sterilized, individually packed

• 82421
  μ-Plate 24Well Black ID 14mmUncoated: #1.5 polymer coverslip, hydrophobic, sterilized, individually packed

μ-Plate 96Well Black

Cat. No. Description

• 89626
  μ-Plate 96Well Black ibiTreat: #1.5 polymer coverslip, tissue culture treated, sterilized, individually packed

• 89621
  μ-Plate 96Well Black Uncoated: #1.5 polymer coverslip, hydrophobic, sterilized, individually packed

• 89627
  μ-Plate 96Well Black Glass Bottom: #1.5H (170 μm ±5 μm) D 263 M Schott glass, sterilized, individually packed

μ-Plate 384Well Black

Cat. No. Description

• 88407 μ-Plate 384Well Black Glass Bottom: #1.5H (170 μm ±5 μm) D 263 M Schott glass, sterilized, individually packed

For research use only!
Further information can be found at ibidi.com. For questions and suggestions please contact us by e-mail [email protected] or by telephone+49 (0)89/520 4617 0.
© ibidi GmbH, Lochhamer Schlag 11, 82166 Gr¨afelfing, Germany.

References

• ibidi – cells in focus
• ibidi – cells in focus

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