ibidi Live Cell Imaging Stage Top Incubators Owner’s Manual

ibidi Live Cell Imaging Stage Top Incubators Owner’s Manual

Live Cell Imaging

Control Temperature, Humidity, and
CO2 / O2 With ibidi Stage Top Incubators

• Universal fit: easy installation on inverted microscopes
• Brilliant images: high-end microscopy with maximal xyz-stability
• Precision control: no evaporation or condensation due to feedbackcontrolled humidity regulation
• Flexible formats: available for slides, dishes, and multiwell plates
• Designed and manufactured in Germany

The Stage Top Incubator from ibidi delivers superior thermal stability and enables you to work with high humidity.

This is indispensable for long-term studies and not possible with other systems.

Download a detailed
Application Guide at: ibidi.com/LiveImagingGuide

Live Cell Imaging Under Physiologic Conditions

Establish in Vivo-Like Conditions on Every Inverted Microscope Cells react sensitively to changes in their environment. For reproducible, biologically relevant results, it is crucial to maintain stable conditions on the microscope during live cell imaging. The ibidi Stage Top Incubators precisely control essential parameters such as temperature, humidity, and CO2 / O2 levels.

Benefits

• Easy installation and use
  Quick mounting on inverted microscopes; to be used with slides, dishes, and multiwell plates

• No evaporation during long-term assays
  Very high humidity levels inside the incubation chamber due to active, feedback-controlled humidity regulation, preventing evaporation and condensation

• Optimal for high-resolution microscopy Maximal xyz-stability on the microscope stage; system can be extended with the ibidi Objective Heater Universal for oil and water immersion

Optional: Objective Heater

Perform long-term oil immersion or water immersion imaging without cooling of the sample.

Applications

• Tube formation / angiogenesis assays
• 2D and 3D chemotaxis assays
• Wound healing and migration assays
• Hypoxia / physioxia assays
• Cell culture under flow assays

Selected Publications

M.S. Shim, et al. Primary cilia and the reciprocal activation of AKT and SMAD2/3 regulate stretch-induced autophagy in trabecular meshwork cells. PNAS, 2021,10.1073/pnas.2021942118

R.G. Willaert, et al. Single yeast cell nanomotions correlate with cellular activity. Science Advances, 2020, 10.1126/sciadv.aba3139

C.A. Reissaus, et al. A Versatile, Portable Intravital
Microscopy Platform for Studying Beta-cell Biology In
Vivo. Scientific Reports, 2019,
10.1038/S41598-019-44777-0

Contact ibidi for a free demo of the ibidi Stage Top Incubator.

The ibidi Stage Top Incubators Technical Innovation

Precise, Feedback-Controlled Humidity

The ibidi Humidity Control ensures a constant and very high relative humidity (RH) level inside the ibidi Stage Top Incubator—identical to the conditions in standard cell culture incubators. This unique and patent-protected technology actively humidifies the gas mixture in a fast and reliable way before it enters the Stage Top Incubator.

• Control range: 20–99% (lower limit is dependent on the environment and gas supply, minimum set humidity is 0%)
• Humidity sensor close to the sample
• Using absolute humidity for the feedback control enables an even more precise RH regulation inside the Incubation Chamber

Accurate CO2 and O2 Control

• CO2 control range: 0–15%
• O2 control range from 1–21% (settings below 1% are less accurate, a typical minimum value in an ibidi Incubation Chamber is 0.5%)

Stable and Homogeneous Temperature

• Temperature control via continuous current, preventing position fluctuations during highresolution live cell microscopy

Stable and consistent temperature distribution in the μ-Slide 8 Well high (left) and the ibidi μ-Dish 35 mm, high (right) during the incubation in the ibidi Heating System Slide/ Dish – Silver Line. Images were acquired with a FLIR thermal camera.

The ibidi Stage Top Incubators Product Variations

ibidi GmbH | Lochhamer Schlag 11 | 82166 Gräfelfing | Germany
Tel.: +49 89 / 520 46 17 – 0 | Fax: +49 89 / 520 46 17 – 59 | E-Mail: [email protected]

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References

• ibidi – cells in focus

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