hygiena Prep Xpress Consumables Guide User Guide
- June 3, 2024
- Hygiena
Table of Contents
Hygiena™ Prep Xpress Consumables Guide
Hygiena™ Prep Xpress
User Guide
Hygiena’s modular Prep Xpress provides liquid handling automation for a
variety of PCR processes.
The Consumables Guide below details the required, protocol-specific
accessories.
Prep Xpress Consumables Guide
| Protocol | Accessory | Description | Total | SKU |
|---|---|---|---|---|
| All Protocols | Plate | Hygiena Cluster Tube Green Rack Adapter, 1.1 mL | ||
| 10 (10 included in startup pack) | MIS2103 | |||
| Prep-Lysis Solution (6 racks) | Reservoir | Vblock 200 mL Open | ||
| Reservoir, V-bottom, Clear PP | 1 | MIS2104 | ||
| Tips | 300 µL Framed Conductive Filtered (5 racks per sleeve – 12 sleeves per | |||
| case) | Dependent on Volume | MIS2082 | ||
| Prep-BHI Solution (5 racks) | Reservoir | Seahorse-Agilent, Reservoir, 1 | ||
| cavity, 8 rows, 290 mL | 1 | MIS2111 | ||
| Tips | 1,000 µL Framed Conductive Filtered (5 racks per sleeve – 8 sleeves per | |||
| case) | Dependent on Volume | MIS2108 |
Transfer-5 uL Cluster Tube to Cluster Tube
Transfer-20 uL Cluster Tube to Cluster Tube| Pedestal| Conductive Tip
Pedestal| 1(1 included in startup pack)| MIS2113
Tips| 50 µL Framed Conductive Filtered (5 racks per sleeve – 12 sleeves per
case)| Dependent on Volume| MIS2107
Hydrate-30 uL-Lysate to PCR
Hydrate-50 uL-Lysate to PCR
Hydrate-30 uL-Lysate to PCR SINGLE
Hydrate-50 uL-Lysate to PCR SINGLE| Racks| Eppendorf PCR Cooler, 200
µl, Strip-Well, PCR, White Well| 2| MIS2109
Tips
| 50 µL Framed Conductive Filtered (5 racks per sleeve – 12 sleeves per case)|
Dependent on Volume| MIS2107
Pedestal| Conductive Tip Pedestal| 1(1 included in startup pack)| MIS2113
Hygiena™Prep Xpress
Tip Utilization Guide
Hygiena’s modular Prep Xpress provides liquid handling automation for a variety of PCR processes utilizing protocol-specific tips. The Tip Utilization Guide below details the tip volumes, applications, and throughput recommendations.
| Tip Volume | 50 µL (MIS2107) |
|---|---|
| Protocol | **Transfer-Cluster tube to Cluster tube & Hydrate-Lysate to |
PCR
Tips Per Sleeve| 1 sleeve = 5 racks of 96 tips in 8×12 strips (480 tips)
Sleeve Throughput| 1 Rack (96 tips ) = 96 samples (1 rack transfer or
hydrate)
1 Strip (8 tips) = 8 samples
1 Sleeve (480 tips) = 480 samples (5 racks transfer or hydrate)
Tips Per Case| 1 Case (5,760 tips) = 12 sleeves of 5 racks of 96 tips in
8×12 strips
Case Throughput| 1 Case = 5,760 samples (60 racks transfer or hydrate)
Tip Volume| 300 µL (MIS2082)
---|---
Protocol| Prep-Lysis Solution (6 racks)
Tips Per Sleeve| 1 sleeve = 5 racks of 96 tips in 8×12 strips (480 tips)
Sleeve Throughput| 1 Strip (8 tips) = 576 samples (6 racks lysis buffer)
1 Rack (96 tips ) = 6,912 samples (72 racks lysis buffer)
1 Sleeve (480 tips) = 34,360 samples (360 racks lysis buffer)
Tips Per Case| 1 Case (5,760 tips) = 12 sleeves of 5 racks of 96 tips in
8×12 strips
Case Throughput| 1 Case = 414,720 samples (4,320 racks lysis buffer)
Tip Volume| 1000 µL (MIS2108)
---|---
Protocol| Prep-BHI Solution (5 racks)
Tips Per Sleeve| 1 sleeve = 5 racks of 96 tips in 8×12 strips (480 tips)
Sleeve Throughput| 1 Strip (8 tips) = 480 samples (5 racks BHI solution)
1 Rack (96 tips ) = 5,760 samples (60 racks BHI solution)
1 Sleeve (480 tips) = 28,000 samples (300 racks BHI solution)
Tips Per Case| 1 Case (3,840 tips) = 8 sleeves of 5 racks of 96 tips in
8×12 strips
Case Throughput**| 1 Case = 230,400 samples (2,400 racks BHI solution)
For additional Prep Xpress protocol information or technical support contact:
diagnostics.support@hygiena.com
For pricing or ordering information, contact your local Hygiena customer
service or sales representative, or contact us on our website.
www.hygiena.com
INS2043-REVA