hygiena Prep Xpress Consumables Guide User Guide

June 3, 2024
Hygiena

Hygiena™ Prep Xpress Consumables Guide
Hygiena™ Prep Xpress
User Guide

Hygiena’s modular Prep Xpress provides liquid handling automation for a variety of PCR processes.
The Consumables Guide below details the required, protocol-specific accessories.

Prep Xpress Consumables Guide

Protocol Accessory Description Total SKU
All Protocols Plate Hygiena Cluster Tube Green Rack Adapter, 1.1 mL
10 (10 included in startup pack) MIS2103
Prep-Lysis Solution (6 racks) Reservoir Vblock 200 mL Open
Reservoir, V-bottom, Clear PP 1 MIS2104
Tips 300 µL Framed Conductive Filtered (5 racks per sleeve – 12 sleeves per
case) Dependent on Volume MIS2082
Prep-BHI Solution (5 racks) Reservoir Seahorse-Agilent, Reservoir, 1
cavity, 8 rows, 290 mL 1 MIS2111
Tips 1,000 µL Framed Conductive Filtered (5 racks per sleeve – 8 sleeves per
case) Dependent on Volume MIS2108

Transfer-5 uL Cluster Tube to Cluster Tube
Transfer-20 uL Cluster Tube to Cluster Tube| Pedestal| Conductive Tip Pedestal| 1(1 included in startup pack)| MIS2113
Tips| 50 µL Framed Conductive Filtered (5 racks per sleeve – 12 sleeves per case)| Dependent on Volume| MIS2107
Hydrate-30 uL-Lysate to PCR
Hydrate-50 uL-Lysate to PCR
Hydrate-30 uL-Lysate to PCR SINGLE
Hydrate-50 uL-Lysate to PCR SINGLE| Racks| Eppendorf PCR Cooler, 200 µl, Strip-Well, PCR, White Well| 2| MIS2109

Tips

| 50 µL Framed Conductive Filtered (5 racks per sleeve – 12 sleeves per case)| Dependent on Volume| MIS2107
Pedestal| Conductive Tip Pedestal| 1(1 included in startup pack)| MIS2113

Hygiena™Prep Xpress
Tip Utilization Guide

Hygiena’s modular Prep Xpress provides liquid handling automation for a variety of PCR processes utilizing protocol-specific tips. The Tip Utilization Guide below details  the tip volumes, applications, and throughput recommendations.

Tip Volume 50 µL (MIS2107)
Protocol **Transfer-Cluster tube to Cluster tube & Hydrate-Lysate to

PCR
Tips Per Sleeve| 1 sleeve = 5 racks of 96 tips in 8×12 strips (480 tips)
Sleeve Throughput| 1 Rack (96 tips ) = 96 samples (1 rack transfer or hydrate)
1 Strip (8 tips) = 8 samples
1 Sleeve (480 tips) = 480 samples (5 racks transfer or hydrate)
Tips Per Case| 1 Case (5,760 tips) = 12 sleeves of 5 racks of 96 tips in 8×12 strips
Case Throughput| 1 Case = 5,760 samples (60 racks transfer or hydrate)
Tip Volume| 300 µL (MIS2082)
---|---
Protocol| Prep-Lysis Solution (6 racks)
Tips Per Sleeve| 1 sleeve = 5 racks of 96 tips in 8×12 strips (480 tips)
Sleeve Throughput| 1 Strip (8 tips) = 576 samples (6 racks lysis buffer)
1 Rack (96 tips ) = 6,912 samples (72 racks lysis buffer)
1 Sleeve (480 tips) = 34,360 samples (360 racks lysis buffer)
Tips Per Case| 1 Case (5,760 tips) = 12 sleeves of 5 racks of 96 tips in 8×12 strips
Case Throughput| 1 Case = 414,720 samples (4,320 racks lysis buffer)
Tip Volume| 1000 µL (MIS2108)
---|---
Protocol| Prep-BHI Solution (5 racks)
Tips Per Sleeve| 1 sleeve = 5 racks of 96 tips in 8×12 strips (480 tips)
Sleeve Throughput| 1 Strip (8 tips) = 480 samples (5 racks BHI solution)
1 Rack (96 tips ) = 5,760 samples (60 racks BHI solution)
1 Sleeve (480 tips) = 28,000 samples (300 racks BHI solution)
Tips Per Case| 1 Case (3,840 tips) = 8 sleeves of 5 racks of 96 tips in 8×12 strips
Case Throughput**| 1 Case = 230,400 samples (2,400 racks BHI solution)

For additional Prep Xpress protocol information or technical support contact: diagnostics.support@hygiena.com
For pricing or ordering information, contact your local Hygiena customer service or sales representative, or contact us on our website.

www.hygiena.com
INS2043-REVA

References

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