dynamic BIOSENSORS HK-EA-2 RNA Enzyme Activity Kit User Manual
- September 6, 2024
- dynamic BIOSENSORS
Table of Contents
dynamic BIOSENSORS HK-EA-2 RNA Enzyme Activity Kit
Key Features
- ssRNA template (48 + 32 bp) for functionalization of heliX
- Adapter Chip on Spot 1.
- dsDNA-RNA template (48 + 32 bp) for functionalization of heliX
- Adapter Chip on Spot 2.
- Compatible with heliX Adapter Chip.
- Includes Adapter strands for 100 regenerations.
- This RNA template carries a moderately hydrophilic red dye (Ra) with a single positive net charge.
Helix Adapter Chip Overview
- 2 spots with 2 different anchor sequences for RNA-encoded addressing.
Product Description
Order Number: HK-EA-2
Table 1. Contents and Storage Information
For research use only.
This product has a limited shelf life, please see the expiry date on the
label. To avoid many freeze-thaw cycles please aliquot the nanolever.
Preparation
IMPORTANT
Both Adapter strands are already pre-hybridized. Adapter strand 1 to the
Template-32-PRa RNA strand, leaving the upper part as ssDNA, and cTemplate-
Adapter strand 2 to the Template-32-PRa RNA strand, leaving Spot 2 completely
as dsDNA-RNA. Next, simply mix in the same vial the Adapter strand 1 – nf /
Template-32-P-Ra RNA (200/250 nM) and the template Adapter strand 2 – nf /
Template-32-P-Ra RNA (200/250 nM) at a 1:1 ratio (v/v). The solution is ready
to use for biochip functionalization.
Useful Order Numbers
Table 2. Order Numbers
Assay Setup in heliOS
For studying enzymatic activity of a nucleic acid modifying enzyme (e.g.,
polymerase, reverse transcriptase, helicase, etc.). Go to heliOS > create a
New Assay Workflow > add Custom Assay > load Enzyme Binding and Activity >
modify the parameters based on your needs and run the assay. Suggested assay
parameters (e.g., flow rates, functionalization time, LED power, etc.) are
within the heliOS assay.
TIP
Is strongly recommended to perform binding kinetics of the enzyme beforehand.
The obtained Kd during enzyme kinetics can be the initial test concentration
for the association of the enzyme during enzyme kinetics. This concentration
is a good compromise to not overcrowd the surface and avoid multiple enzymes
binding to the same template.
TIP
For an initial scouting of the substrate, choose a broad concentration
splitting spanning the low nanomolar to high micromolar, and a blank. A
minimum of 6 concentrations of the substrate are recommended to obtain a
reliable sigmoidal fit during the extraction of the KM. For inhibition assay,
analysis, or any further questions, please contact the support team at
support@dynamicbiosensors.com.
Contact
Dynamic Biosensors GmbH
Perchtinger Str. 8/10
81379 Munich
Germany
Dynamic Biosensors, Inc.
300 Trade Center, Suite 1400
Woburn, MA 01801
USA
Order Information order@dynamic-biosensors.com
Technical Support support@dynamic-biosensors.com
www.dynamic-biosensors.com
Instruments and chips are engineered and manufactured in Germany.
2024 Dynamic Biosensors GmbH | Dynamic Biosensors, Inc. All rights reserved.
References
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