dynamic BIOSENSORS AS-1 Adapter Strand 1 User Manual

August 28, 2024
dynamic BIOSENSORS

dynamic BIOSENSORS AS-1 Adapter Strand 1

Key Features

  • Adapter strand 1 – Rc for functionalization of heliX® Adapter Chip Spot 1.
  • Compatible with heliX® Adapter Chip.
  • Includes Adapter strands for 50 regenerations.
  • Ideal for MIX&RUN sample preparation.
  • Adapter strand 1 carries a moderately hydrophobic red dye (Rc) with a neutral net charge.

heliX® Adapter Chip Overview

2 spots with 2 different anchor sequences for DNA-encoded addressing.

Product Description

Order Number: AS-1-Rc
Table 1. Contents and Storage Information

Material Cap Concentration Amount Buffer Storage
Adapter strand 1 – Rc Black 400 nM 5 x 100 µL TE40 [1] -20°C

For research use only.
This product has a limited shelf life, please see expiry date on label..
To avoid many freeze thaw cycles please aliquot the nanolever.

Preparation | MIX&RUN

In-solution hybridization of adapter and ligand strands:

  1. Mix Adapter strand 1 – Rc (400 nM) and conjugated Ligand strand (500 nM) at 1:1 ratio (v/v).
  2. Incubate the solution of step 1 at RT at 600 rpm for 30 min to ensure complete hybridization.
  3. Mix solution of step 2 and Adapter strand 1 – Rc – lfs (200 nM) at 1:1 ratio (v/v).
    Solution is ready to use for biochip functionalization.
    Stability of the solution is related to the stability of the ligand molecule.

Table 2. Additional material for functionalization of spot 1 and reference spot 2.

Material Concentration Buffer Related Product Name Order No

Ligand strand

carrying the conjugated ligand

| 500 nM| 2P] E40 [| heliX® Amine Coupling Kit 1| HK-NHS-1
Adapter strand 1 – Rc – lfs| 200/250 nM| TE40 [1]| Adapter strand 2 with red dye Rc

prehybridized with ligand-free strand

| AS-2-Rc-lfs

Example
Required volume for 3 functionalizations: 100 μL with a final concentration of 100 nM.

Vial 1 Vial 2

Adapter strand 1 – Rc

(400 nM)

| Conjugated Ligand strand

(500 nM)

| Adapter strand 1 – Rc – lfs

(200/250 nM)

25 µL| 25 µL| 50 µL

After incubation time, mix vial 1 and vial 2 to obtain 100 μL of ready-to-use DNA solution.

Contact

Dynamic Biosensors GmbH
Perchtinger Str. 8/10
81379 Munich
Germany

Dynamic Biosensors, Inc.
300 Trade Center, Suite 1400
Woburn, MA 01801
USA

Order Information order@dynamic-biosensors.com
Technical Support support@dynamic-biosensors.com
www.dynamic-biosensors.com

Instruments and chips are engineered and manufactured in Germany.
©2024 Dynamic Biosensors GmbH | Dynamic Biosensors, Inc. All rights reserved.

  1. TE40: 10 mM Tris, 40 mM NaCl, 0.05 % Tween20, 50 μM EDTA, 50 μM EGTA
  2. If the protein is not stable in PE40 (TE40, HE40), please check buffer compatibility with the switchSENSE® compatibility sheet.

www.dynamic-biosensors.com

References

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