dynamic BIOSENSORS heliX plus Adapter Strand 1 User Manual

August 27, 2024
dynamic BIOSENSORS

dynamic BIOSENSORS heliX plus Adapter Strand 1

Specifications:

  • Product Name: heliX+
  • Model: ADAPTER STRAND 1 with red dye Ra
  • Manufacturer: Dynamic Biosensors GmbH & Inc.
  • Order Number: AS-1-Ra
  • Concentration: 400 nM
  • Storage: Black cap, Buffer TE40
  • For research use only

Product Description:

The heliX+ ADAPTER STRAND 1 is designed with red dye Ra and features 2 spots with 2 different anchor sequences for DNA-encoded addressing. It comes in a concentration of 400 nM and is intended for research purposes.

Usage Instructions:
Preparation | MIX &RUN:

  1. Mix Adapter strand 1 – Ra (400 nM) and conjugated Ligand strand (500 nM) at a 1:1 ratio (v/v).
  2. Incubate the solution from step 1 at room temperature at 600 rpm for 30 minutes to ensure complete hybridization.
  3. Mix the solution from step 2 with Adapter strand 2 – Ra – lfs (200 nM) at a 1:1 ratio (v/v).
  4. The solution is now ready to be used for biochip functionalization.

Note: The stability of the solution is dependent on the stability of the ligand molecule.

FAQ:

  1. Q: What is the shelf life of the heliX+ ADAPTER STRAND 1?
    A: The product has a limited shelf life, please refer to the expiry date on the label.

  2. Q: Can the heliX+ ADAPTER STRAND 1 be frozen?
    A: To avoid many freeze-thaw cycles, it is recommended to aliquot the nanolever.

heliX+
User Manual

ADAPTER STRAND 1
with red dye Rb
Dynamic Biosensors GmbH & Inc.
AS-1-Rb v5.1

Key Features

  •  Adapter strand 1 – Rb for functionalization of heliX® Adapter Chip Spot 1.
  • Compatible with heliX® Adapter Chip.
  • Includes Adapter strands for 50 regenerations.
  • Ideal for MIX&RUN sample preparation.
  • Adapter strand 1 carries a moderately hydrophilic red dye (Rb) with a neutral net charge.

heliX® Adapter Chip Overview
2 spots with 2 different anchor sequences for DNA-encoded addressing.

Product Description

Order Number: AS-1-Rb
Table 1. Contents and Storage Information

Material Cap Concentration Amount Buffer Storage
Adapter strand 1 – Rb Black 400 nM 5 x 100 µL TE40 [ 1]

-20°C

For research use only.

  • This product has a limited shelf life, please see expiry date on label.
  • To avoid many freeze thaw cycles please aliquot the nanolever

Preparation | MIX&RUN

In-solution hybridization of adapter and ligand strands:

  1.  Mix Adapter strand 1 – Rb (400 nM) and conjugated Ligand strand (500 nM) at 1:1 ratio (v/v).
  2.  Incubate the solution of step 1 at RT at 600 rpm for 30 min to ensure complete hybridization.
  3.  Mix solution of step 2 and Adapter strand 1 – Rb – lfs (200 nM) at 1:1 ratio (v/v). Solution is ready to use for biochip functionalization. Stability of the solution is related to the stability of the ligand molecule

Table 2. Additional material for functionalization of spot 1 and reference spot 2.

Material Concentration Buffer Related Product Name Order No

Ligand strand

carrying the conjugated ligand

| 500 nM| P 2] E40 [| heli X ® Amine Coupling Kit 1| HK-NHS-1
Adapter strand 1 – Rb – lfs| 200/250 nM| TE40 [ 1]| Adapter strand 2 with red dye Rb prehybridized with ligand-free strand| AS-2 -Rb-lfs

Example
Required volume for 3 functionalizations: 100 μL with a final concentration of 100 nM.

Vial 1 Vial 2

Adapter strand 1 – Rb

(400 nM)

| Conjugated Ligand strand (500 nM)| Adapter strand 1 – Rb – lfs (200/250 nM)
25 µL|  25 µL| 50 µL

After incubation time, mix vial 1 and vial 2 to obtain 100 µL of ready-to-use DNA solution.

Contact
Dynamic Biosensors GmbH

  • Perchtinger Str. 8/10
  • 81379 Munich
  • Germany

Dynamic Biosensors, Inc.

  • 300 Trade Center, Suite 1400
  • Woburn, MA 01801
  • USA

Order Information order@dynamic-biosensors.com
Technical Support support@dynamic-biosensors.com

www.dynamic-biosensors.com
Instruments and chips are engineered and manufactured in Germany. ©2024 Dynamic Biosensors GmbH | Dynamic Biosensors, Inc. All rights reserved.

  1. TE40: 10 mM Tris, 40 mM NaCl, 0.05 % Tween20, 50 μM EDTA, 50 μM EGTA
  2. If the protein is not stable in PE40 (TE40, HE40), please check buffer compatibility with the switchSENSE® compatibility sheet.

www.dynamic-biosensors.com

References

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