hygiena KIT230015 Internal Amplification Control User Guide

July 30, 2024
Hygiena

Internal Amplification Control
Ready Reference Guide
Revision A, February 2024
Product No. KIT230015

The Internal Amplification Control contains a suitable internal control for colony confirmation procedures with Hygiena Diagnostics‘ real-time PCR LyoKits. Colony confirmation procedures which include shortened PCR cycler programs (e.g., 30 cycles instead of 50), require the use of additional internal control DNA.

KIT CONTENTS
  3 x 700 µL

PROTOCOL

Use 20 µL per LyoKit reaction. Add 5 µL of bacterial cells (colony) suspended in microproof® Suspension Buffer (Product No. KIT230178). Run with a shortened PCR program (30 cycles). More details can be found in the product instruction for each respective LyoKit.

The Internal Amplification Control can be used with the following kits:
foodproof® STEC Identification LyoKit (Product Nos. KIT230079, KIT230080)
foodproof® Salmonella Enteritidis & Typhimurium Detection LyoKit (Product Nos. KIT230106, KIT230107)
foodproof ® Salmonella Genus plus Enteritidis & Typhimurium Detection LyoKit (Product Nos. KIT230134, KIT230135, KIT230136)
foodproof ® Vibrio Detection LyoKit (Product Nos. KIT230117, KIT230118)
microproof ® Legionella Quantification LyoKit (Product Nos. KIT230119, KIT230120)
vetproof® SE Vaccine Detection 1 Kit (Product Nos. KIT230193, KIT230194) and others (Please enquire)

Internal Amplification Control
KIT230015
Store kit at 2 to 8 °C
For food testing purposes
FOR IN VITRO USE ONLY
Made in Germany
Hygiena® | Camarillo, CA 93012 USA | diagnostics.support@hygiena.com | www.hygiena.com

References

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