QIAGEN HB-2616-002 PowerBead Pro Plates User Guide
- June 6, 2024
- QIAGEN
Table of Contents
QIAGEN HB-2616-002 PowerBead Pro Plates
Procedure
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Spin the PowerBead Pro Plate (cat. no. 19311) briefly to ensure that the beads have settled at the bottom. Remove and discard the square well mat from the bead plate.
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Add sample material and lysis buffer according to the instructions in the respective kit handbook.
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Make sure to remove any residual liquid on top of the plate.
Note: Liquid on top of the plate will prevent a tight sealing of the plate with the sealing film. This may result in leakage during disruption in the TissueLyser II (cat. no. 85300). -
Secure the sealing film (provided) tightly onto the bead plate. Use a tool such as a scraper or plate roller to make the sealing film adhere firmly onto the plate.
Note: A strong seal is essential to prevent leakage during disruption in the TissueLyser II. Usage of a mechanical plate sealer can be advantageous for a consistent and a uniform seal. -
Put the silicone compression mat (provided) on top of the bead plate that is sealed with the sealing film.
Note: Two silicone compression mats are provided so that 2 plates can be processed in parallel in the TissueLyser II. The mats are reusable for the remaining plates. -
Place this entire assembly (from steps 1 to 5) between 2 Plate Adapter Sets (cat. no. 11990) for disruption in the TissueLyser II.
Important: When using this assembly, do not exceed the recommended disruption time and setting of 2 x 5 min at 25 Hz, because extended processing might lead to leakage.
For up-to-date licensing information and product-specific disclaimers, see the respective QIAGEN® kit handbook or user manual. QIAGEN kit handbooks and user manuals are available at www.qiagen.com or can be requested from QIAGEN Technical Services or your local distributor.
Trademarks: QIAGEN®, Sample to Insight® (QIAGEN Group). Registered names, trademarks, etc. used in this document, even when not specifically marked as such, are not to be considered unprotected by law. 1125027 07/2021 HB-2616-002 © 2021 QIAGEN, all rights reserved.
