NEUATION iFuge M15K High-Speed Microcentrifuge User Manual

iFUGE M15K
PRODUCT User Manual

INTRODUCTION

This manual provides important safety information for this centrifuge. It should be kept near the centrifuge for quick & easy reference. This centrifuge is equipped with a maintenance-free brushless DC motor drive with microprocessor-controlled and a safety feature of imbalance detection. The centrifuge is designed for high-speed spin downs applications. Multiple rotor options make it usable for a wide range of applications.

 INTEN5DED USE

This centrifuge is designed for the separation of solid suspensions in liquids such as cells, and macromolecules such as DNA, RNA & proteins. This centrifuge can be used in various research laboratories & institutions.

FEATURES

• Maximum volume of 12 x 2 ml
• Imbalance detector with auto cutoff for safe operation
• Brushless DC motor for maintenance-free performance
• Lid lock safety-the lid opens automatically on run completion
• Digital display with speed and time indicator
• RPM setting from 500-15000 RPM with a count of 100
• One press changeover from RPM to RCF functionality
• Reduction adaptors for 0.1/0.2 & 0.5ml tubes
• Emergency lid release during power failure
• Automatic internal diagnosis & error display

NOTE: Before using the instrument, please read this user manual carefully. This user manual is intended to assist with the operation and care of the unit only and not its repair. For repair please contact the supplier.

TECHNICAL SPECIFICATION

STANDARD ACCESSORIES

• Power Adaptor: 100-240 VAC, 50/60 Hz, lamp (Input), 24 V
• 4A (Output)
• 12 – slot rotor (Pre-installed) with reduction adaptors for 0.1/0.2 & 0.5ml tubes
• T-Allen Key
• Product user manual & warranty card
• PCR strip rotor (optional)

ROTOR AND REDUCED ADAPTORS

The below table shows the Maximum RCF of the rotor and Maximum RCF with different tubes & adaptors.

12 tubes rotor
**| 1.5/2.0m1 tubes**

| 0.5ml Adaptor

| 0.1/0.2m1 Adaptor

---|---|---|---
1.5/2 ml| 0.5 ml| 0.4 ml| 0.2 ml| 0.1 ml
Max. Speed| 15000| 15000| 15000| 15000| 15000
Max. RCF| 15596| 12577| 12074| 11105| 10278
PCR strip rotor (Optional)

|

0.1/0.2m1 PCR strip

---|---
Max. Speed| 15000
Max. RCF| 13080

NOTE: Always use recommended accessories for best results & product safety.

SAFETY PRECAUTIONS

• Never use the centrifuge in any manner not specified in this manual.

• Using equipment in any manner not specified in this manual or by the manufacturer will void the warranty.

• Never move the centrifuge while the rotor is spinning.

• The rotor and the rotor lid must always be securely fastened. If the centrifuge makes an unusual noise during operation, the rotor or rotor lid fit needs to be checked. Switch OFF the device immediately by pressing STOP, check rotor fit & fasten it well.

• The rotors must be loaded symmetrically. Each tube should be counterbalanced by another tube of the same weight.

• Do not use the centrifuge or rotor that has not been correctly installed or damaged.

• Repairs must only be performed by an authorized service technician.

• Using incorrect rotors & wrong spare parts will void the warranty.

• The centrifuge may be used for the specified applications only. It must not be operated in a hazardous or flammable environment and must not be used A to centrifuge explosive or highly reactive substances.

• If liquids are spilled on the rotor or rotor chamber, the centrifuge must be cleaned carefully and properly before being used again.

• Prior to centrifugation, the tubes should be visually inspected for material damage. Damaged tubes may not be centrifuged. This is because broken tubes can, in addition to sample loss, create an imbalance which can result in further damage to the centrifuge and accessories.

• The maximum capacity of tubes must not exceed its corresponding rotor capacity. Do not use liquid with a density higher than 1.2g/ml for full load operation.

• Do not lean on the equipment. It may damage the equipment or harm the operator.

• When moving the centrifuge from a cold room to a normal room, run the centrifuge for 30 minutes beforehand in the cold room to avoid condensation. Alternately, allow it to warm up in the lab for at least 3 hours before use, but do not plug in the centrifuge in order to prevent possible damage by condensation.

• Be sure to close the lid of the tube tightly prior to centrifugation. The open tube lid can be torn off during centrifugation and can damage the rotor lid or centrifuge.

• Rotors and rotor lids are high-graded components that are subject to extreme mechanical strain. Even slight scratches and tears can lead to serious internal material damage. Ensure to check the rotor for any signs of damage before use. Rotor & rotor lid showing visible signs of corrosion or mechanical damage should not be used, contact your local supplier or mfg for replacement rotor & parts.

• Do not fill tubes while they are in the rotor. Liquid spillage may harm the device.

• In the event of contamination caused by aggressive agents, the rotor must be
  cleaned immediately using a natural cleaning liquid (like water). This is particularly important for the bores of the tubes. If any damage is seen, contact the service technician.

• Before using cleaning or decontamination methods, other than those stipulated by the manufacturer, contact the manufacturer to ensure that the intended method will not damage the centrifuge.

• The power cord given with a centrifuge unit is designed to use for that particular centrifuge. Do not use any other power cord, using any other power cord /adaptor may damage the centrifuge and will void the warranty.

INSTALLATION

Gently remove the upper packaging and take out the centrifuge by holding it from the bottom. When the centrifuge is used for the first time, ensure that all the packaging and accessories are removed from the rotor chamber. The rotor is pre-installed. Please make sure that the rotor is firmly tightened. Accessories and user manual are kept inside the box with the centrifuge. Please keep all the packaging in safe storage for at least 2 years for warranty purposes.
The following points are important for proper installation:

8.1 CONNECTING POWER ADAPTOR

1. Connect one side of power adaptor to the rear side of the centrifuge and other to supply as shown in the figure below.

2. Rotate clockwise the adaptor nut to tighten the adaptor with the centrifuge. Ensure the power switch is OFF while connecting the power adaptor.
   

3. The sticker on the body & jack are indicating the position of the rib on the jack. While fitting the adaptor to the unit-please match the sticker position as shown in the figure below & gently push it in

4. If matched correctly the plug will go in easily without much force (do not try to force the plug in if it is not matched properly). Once the plug is inserted into position, turn the loose upper ring clockwise to tighten & to secure the fitment.

ROTOR INSTALLATION

The rotor of the centrifuge is pre-installed. In case of any spillage, remove the rotor as shown in the above figure to clean the rotor and its chamber thoroughly.

9.1 REMOVING ROTOR

1. To remove the rotor, first, remove the tube Holders.
2. Using the T-Allen key, loosen the rotor nut by rotating the rotor nut counterclockwise and take the out rotor vertically.

9.2 INSTALLING THE ROTOR

1. Make sure the rotor shaft and the rotor mounting hole are clean. Load the rotor vertically into the motor shaft
2. Put the T-Allen key in the rotor nut & turn clockwise to tighten the rotor
3. After properly fastening the rotor, place the Tube Holders into the rotor.

BALANCING THE ROTOR

Always balance the rotor before centrifugation Symmetrical loading of tubes in the rotor is necessary. Otherwise, vibration or imbalance can occur which can cause serious damage to the centrifuge. The following are processes of symmetrical loading of centrifuge tubes inside the rotor. The samples in the tubes should be of equal volume. To achieve a balanced condition, “Reduction Adaptors” are mandatory to place inside the rotor.
PROPER BALANCED ROTOR IMBALANCED ROTOR

OPERATION

After properly installing the centrifuge, switch ON the centrifuge from the rear side by pressing the power switch. The lid status will be seen in the display. If the lid is open, the display will show “Lid” and if it is closed, the set RPM will be displayed. Please ensure that the Tube Holders are inside the rotor.

  START

After setting parameters such as speed & timer as instructed below press the START button to run the machine.

11.2 STOP/OPEN LID
To stop the machine at any point of time press the “STOP” button. The lid will open automatically. The LID will open on its own once the completion of run. During ramp, no other button will work except for the STOP button.

11.3 SPEED Upon
turning the power on the machine, RPM/RCF display will be of the previous set speed. The speed can be set from 500-15000 RPM. The speed can be set by using the “+” button for increment & “-” button for decrement of the values. The increment/decrement is in the 100 RPM only. The Shown values will be multiples of 10 (x10). The values get saved automatically after blinking for S times.

11.4 RPM-RCF SWITCH
Long Press the RPM/RCF button to change the mode from RPM to RCF. The switch over will be indicated by the LED on the respective mode. While RCF mode is ON, speed is to be read as value x10 in the display window. The maximum RCF of the centrifuge is 15596 xg. Press the speed button to see the corresponding Value.

11.5 TIME
Upon turning the power on the machine, RPM/RCF display will be of the previous set timer. The timer can be set from 1 minute to 99 minutes & infinite mode. The timer can be set by using the increment (+) and decrement (-) buttons. The set timer will automatically get saved. The timer works as a countdown which means the timer will run in descending order during the operation of the machine.

11.6 SHORT-SPIN OPERATION
The centrifuge can be operated for a short run by pressing and holding the SHORT SPIN button. The centrifuge will continue to run as long as the Short- Spin button is pressed and the time in seconds will count up for 1 minute after 1 minute time count up in minute on the time display. After releasing short spin button the time in the display shows the duration of the short spin. The Short-Spin speed is based on the last run settings remaining in the memory or set rotational speed prior to short spin as required.

11.7 INCREMENT / DECREMENTS
Press “+” and “-” buttons to increase and decrease the speed or time values normally.

11.8 OPENING THE CENTRIFUGE LID IN CASE OF POWER FAILURE

Disconnect the centrifuge from the main supply. Wait until the rotor comes to a standstill (this may take some time). Once the rotor has stopped, pull out the emergency lid release as shown in the figure. This will open the centrifuge lid. After the centrifuge lid opens, push the thread back in the hole with your hand and fit the emergency release cap back in the hole.

11.9 IMBALANCE DETECTION
This centrifuge is equipped with an imbalance detection safety feature. When the rotor is not loaded symmetrically, the imbalance detector gets activated. This will cut off the centrifugation & error “ErrSS” will be shown on the display. To correct the imbalance, open the centrifuge lid and correct the imbalance load using methods described in the balancing the rotor section (page no.4) of this manual. After correctly loading the tubes into the rotor turn off the centrifuge & turn it back on.

SAFETY PRECAUTION

1.  Never use the centrifuge in any manner not specified in the manual.
2. Never move the centrifuge while it’s operating.
3. The rotor must always be securely fastened. If the centrifuge makes an unusual noise during the operation, the rotor fit needs to be checked.
4. The rotor must be loaded symmetrically.
5. Do not use the centrifuge or rotor, if it is not installed correctly or it is damaged.
6. Do not use the centrifuge or rotor if it shows any sign of external mechanical or electrical damage.
7. The centrifuge must be used only for the specified application. It must not be operated in a hazardous or flammable environment & no hazardous or flammable content should be centrifuged.
8. Do not fill tubes while they are in the rotor. Liquid spillage may harm the device.
9. If the liquid is spilled on the rotor or rotor chamber, the centrifuge must be cleaned immediately.
10. “Tube Holders” are a part of the Rotor. They should be placed in the rotor whether 2, 3,4, 6…12 tubes are being used.

TROUBLESHOOTING

The centrifuge has a self-diagnostic function. If a problem occurs, an error/warning code will be displayed on display screen and the operator can determine the malfunction with the warning code below.

adaptor at both ends
Power failure| Check the main fuse of the lab.
Improper connection| Connect adaptor properly
Lid open| The lid is closed correctly| Close lid correctly
Error with lid closing and opening mechanism| Contact service representative
Err SS| Rotor not loaded symmetrically| Load the rotor symmetrically & restart the centrifuge
The centrifuge lid cannot be opened| The rotor is still spinning to a stop| Wait for the rotor to come
---|---|---
Power failure| Press emergency lid release after rotor stops
Centrifuge shakes during acceleration &makes loud running noises while running| The rotor is not loaded symmetrically| Load rotor symmetrically & restart operation
Either a broken tube, damage to the rotor or motor is the cause for run noise| Replace broken tube. For

damaged rotor/motor contact a service representative

Rotor is damaged| Remove & change the rotor
Display error Err 1| Loose connection of display latch motor damaged, Latch jammed or any Limit switch of latch got damaged| Contact service representative
Err 52| Rotor is Stuck| Turn OFF the centrifuge, Check & fit the rotor properly & turn ON the centrifuge
Power tripping| The cable is not fit properly| Remove the cable and connect properly
Last run memory not displayed| Turing ON centrifuge immediately after turning it OFF| Maintain 3 seconds gap Between switching OFF and switching ON again
System gets hang| Electronics error| Switch off the centrifuge and then switch it ON again.If the error still shows, contact the service representative

IMPORTANT NOTE :

1. If the centrifuge is working slow or hot due to overdraw current, restart the centrifuge and check it again.
2. Do not use liquid with density higher than 1.2g/m1for full load operation.

MAINTENANCE AND CLEANING

1. The rotor and the outside of the centrifuge should be cleaned regularly with a moist cloth.
2. Ensure that while cleaning the unit it is not plugged in.
3. The brushless motor in the centrifuge does not require routine maintenance. Any required service should be performed by authorized, qualified personnel only. Repairs performed by unauthorized personnel may void the warranty.
4. Always keep the centrifuge housing, rotor chamber, rotor, and rotor accessories clean. All parts should be wiped down periodically with a soft cloth. For more thorough cleaning, use a neutral cleaning agent (Ph between 6 and 8) applied with a soft cloth. Excessive amounts of liquid should be avoided. The liquid should not come into contact with the motor.
5. After cleaning, ensure that all parts are dried.
6. Do not place the rotor into the cleaning solution!

WARRANTY STATEMENT

This product is warranted to be free from defects in material and workmanship for a period of two (2) years from the date of purchase. Your product will be duly repaired upon prompt notification in compliance with the following conditions: This warranty is valid only if the product is used for its intended purpose and within the guidelines specified in this instruction manual. This warranty does not cover damage caused by accident, neglect, misuse, improper service, natural forces or other causes not arising from defects in original material or workmanship. This warranty does not cover any incidental or consequential damages, commercial loss or any other damages from the use of this product.

The warranty is invalidated by any non-factory modification, which will immediately terminate all liabilities on us for the products or damages caused by its use. The buyer and its customer shall be responsible for the product or use of products as well as any supervision required for safety. If requested the products must be returned to the distributor in well packed and insured manner and all shipping charges must be paid.

Some states do not allow limitations on the length of implied warranties or the exclusion or limitation of incidental or consequential damages. This warranty gives you specific legal rights. This warranty is given expressly in lieu of all other warranties, expressed or implied.

The purchaser agrees that there is no warranty of merchantability or of fitness for any intended purpose and that there are no other remedies or warranties, expressed or implied, which extend beyond the description on the face of the agreement. This warranty is only applicable to the original purchaser.

Products received without proper authorization will not be entertained. All items returned for service should be sent postage prepaid in the original packaging or other suitable cartons, padded to avoid damage. We will not be responsible for damage incurred by improper packaging.
All items returned for service should be set postage prepaid in the original packaging or other suitable cartons, added to avoid damage.
This warranty is valid only if the warranty is registered with the supplier within 30 days from the date of purchase.

For your reference, make a note of the serial number, date of purchase, and supplier here.

Serial No:  Purchase Date:
Supplier:

PRODUCT DISPOSAL

In case the product is to be disposed of, the relevant legal regulations are to be observed.
Information on the disposal of electrical and electronic devices in the European Community.
The disposal of electrical devices is regulated within the European Community by national regulations based on EU Directive 2012/19/EU on waste electrical and electronic equipment (WEEE). According to these regulations, any devices supplied after 13.06.05 in the business-to-business sphere, to which this product is assigned, may no longer be disposed of in the municipal or domestic waste. They are marked with the following symbol to indicate this.
As disposal regulations within the EU may vary from country to country please contact your supplier if necessary.

Negation Technologies Pvt. Ltd.
Plot No. 15, GIDC Electronic Park SEZ Kolavada Road, Gandhinagar – 382026, Gujarat, India
Website: www.neuation.com

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