hygiena KIT230200 Staphylococcus Aureus Detection Kit User Guide
- June 1, 2024
- Hygiena
Table of Contents
hygiene KIT230200 Staphylococcus Aureus Detection Kit
PCR kit for the qualitative detection of femA DNA using real-time PCR instruments. Before starting, it is strongly recommended to read the entire product manual available on our website.
PROGRAM SETUP
Program your real-time PCR instrument before setting up the PCR reactions. Select the following channels:
- FAM (femA) and VIC/HEX (Internal Control).
Pre-incubation: 1 cycle
- Step 1: 95 °C for 10 min
Amplifi cation: 40 cycles
- Step 1: 95 °C for 15 sec
- Step 2*: 60 °C for 60 sec
DATA INTERPRETATION
Verify the results of positive (Control Template) and negative (H2 O) controls, before interpreting the sample results. Always compare samples to positive and negative controls. Review data from each channel and interpret the results as described in the table.
PREPARATION OF THE RT-PCR MIX
Take appropriate precautions to prevent contamination, e.g., by using filter
tips and wearing gloves. Thaw reagents, mix (do not vortex!) and briefly spin
vials before opening.
PREPARE PCR MIX
(n samples + 2 controls + at least one additional reaction to cover pipetting loss). Mix carefully but thoroughly by pipetting up and down.
ADD PCR MIX
Pipet 14 µL of prepared PCR mix into each strip or plate well.
ADD SAMPLES AND CONTROLS
Pipet 6 µL of samples, negative control (PCR-grade H2 O, not included) or
Control Template (C) into respective wells.
SEAL
Seal strips/plates accurately.
CENTRIFUGE
Briefly spin strips/plate in a suitable centrifuge.
START REAL-TIME PCR RUN
Cycle samples as described above.
foodproof® SL
Staphylococcus aureus
Detection Kit
KIT230200
Kit for 50 reactions
Store kit at -15 to -25 °C
For food testing purposes
FOR IN VITRO USE ONLY
Hygiena® | Camarillo, CA 93012 USA |
diagnostics.support@hygiena.com |
www.hygiena.com