hygiena KIT230201 Bacillus Cereus Detection Kit User Guide

hygiena KIT230201 Bacillus Cereus Detection Kit

Product Information

Specifications

• Product Name: Bacillus cereus Detection Kit
• Product Number: KIT230201
• Revision: A, December 2023
• Usage: PCR kit for the qualitative detection of groEL target using real-time PCR instruments
• Intended Use: For food testing purposes
• Kit Size: 50 reactions
• For In Vitro Use Only

Product Usage Instructions

Program Setup

1. Program your real-time PCR instrument before setting up the PCR reactions. Select the following channels: FAM (groEL) and VIC/HEX (Internal Control).

Data Interpretation

1. Verify the results of positive (Control Template) and negative (H2O) controls before interpreting the sample results. Always compare samples to positive and negative controls.
2. Review data from each channel and interpret results as described in the table provided.

Preparation of the RT-PCR Mix

1. Take appropriate precautions to prevent contamination, e.g., by using filter tips and wearing gloves. Thaw reagents, mix (do not vortex!) and briefly spin vials before opening.
2. Prepare PCR mix for each reaction to a suitable tube. Mix carefully but thoroughly by pipetting up and down.
3. Add PCR mix, followed by samples and controls.
4. Seal strips/plates accurately.
5. Briefly spin strips/plate in a suitable centrifuge.
6. Start real-time PCR run following the specified cycle for samples.

Frequently Asked Questions (FAQ)

Q: Can this kit be used for purposes other than food testing?

• A: No, this kit is intended for food testing purposes only as indicated in the product information.

Q: How many reactions can be performed with this kit?

• A: The kit allows for 50 reactions to be conducted.
• Product No. KIT230201
• PCR kit for the qualitative detection of groEL target using real-time PCR instruments.
• Before starting, it is strongly recommended to read the entire product manual available on our website.

PROGRAM SETUP

• Program your real-time PCR instrument before setting up the PCR reactions.

Select the following channels:

• FAM (groEL) and VIC/HEX (Internal Control).
• Pre-incubation: 1 cycle
• Step 1: 95 °C for 10 min
• Amplification: 40 cycles
• Step 1: 95 °C for 15 s
• Step 2: 60 °C for 60 s
• Fluorescence detection

DATA INTERPRETATION

• Verify the results of positive (Control Template) and negative (H2O) controls, before interpreting the sample results.
• Always compare samples to positive and negative controls.
• Review data from each channel and interpret results as described in the table.

PREPARATION OF THE RT-PCR MIX

• Take appropriate precautions to prevent contamination, e.g., using filter tips and gloves.
• Thaw reagents, mix (do not vortex!), and briefly spin vials before opening.

1. PREPARE PCR MIX
   • Add 10 µL Master Mix (2XM) and 4 µL Primer/Probe Mix (P)  for each reaction to a suitable tube.
   • (n samples + 2 controls + at least one additional reaction to cover pipetting loss). Mix carefully but thoroughly by pipetting up and down.
2. ADD PCR MIX
   • Pipet 14 µL of prepared PCR mix into each strip or plate well.
3. ADD SAMPLES AND CONTROLS
   • Pipet 6 µL of samples, negative control (PCR-grade H2O, not included) or Control Template (C) into respective wells.
4. SEAL
   • Seal strips/plates accurately.
5. CENTRIFUGE
   • Briefly spin strips/plate in a suitable centrifuge.
6. START REAL-TIME PCR RUN
   • Cycle samples as described above.

• foodproof® SL Bacillus cereus Detection Kit
• KIT230201
• Kit for 50 reactions Store kit at -15 to -25 °C
• For food testing purposes FOR IN VITRO USE ONLY
• Hygiena®
• Camarillo, CA 93012 USA
• [email protected]
• www.hygiena.com

Documents / Resources

| hygiena KIT230201 Bacillus Cereus Detection Kit [pdf] User Guide
KIT230201 Bacillus Cereus Detection Kit, KIT230201, Bacillus Cereus Detection Kit, Cereus Detection Kit, Detection Kit
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References

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