Hygiena KIT230215 GMO A2704-12 Soya Detection Kit User Guide
- May 15, 2024
- Hygiena
Table of Contents
Hygiena KIT230215 GMO A2704-12 Soya Detection Kit
PCR kit for the qualitative detection of A2704-12 DNA using real-time PCR instruments. Before starting, it is strongly recommended to read the entire product manual available on our website.
PROGRAM SETUP
Program your real-time PCR instrument before setting up the PCR reactions. Select the following channels: FAM (A2704-12) and VIC/HEX (Internal Control).
Pre-incubation: 1 cycle
Step 1: 50 °C for 2 min
Step 2: 95 °C for 10 min
Amplification: 45 cycles
Step 1 : 95 °C for 15 sec
Step 2: 60 °C for 60 sec
1 cycle Fluorescence detection
DATA INTERPRETATION
Verify results of positive (Control Template) and negative controls (H2O), before interpreting sample results. Always compare samples to positive and negative control. Review data from each channel and interpret results as described in the table.
| FAM | VIC/HEX | Result Interpretation |
|---|---|---|
| + | + or – | Positive for A2704-12 |
| – | + | Negative for A2704-12 |
| – | – | Invalid |
food proof® is a registered trademark of Hygiena. The above mentioned real- time PCR instruments are registered trademarks of their respective holders.
PREPARATION OF THE PCR MIX
Take appropriate precautions to prevent contamination, e.g., by using filter tips and wearing gloves. Thaw reagents, mix (do not vortex!) and briefly spin vials before opening
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PREPARE PCR MIX
Add 12.5 µL Master Mix (2XM), 4.0 µL Primer/Probe Mix (P) and 3.5 µL PCR- grade H2 O (not included) (n samples + 2 controls + at least one additional reaction to cover pipetting loss). Mix carefully but thoroughly by pipetting up and down. for each reaction to a suitable tube.
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ADD PCR MIX
Pipette 20 µL of prepared PCR mix into each strip or plate well.
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ADD SAMPLES AND CONTROLS
Pipette 5 µL of samples, negative control (PCR-grade H2 O) or Control Template (C) into respective wells.
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SEAL
Carefully seal strips/plate.
-
. CENTRIFUGE
Briefly spin strips/plate in a suitable centrifuge.
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START REAL-TIME PCR RUN
Cycle samples as described above.
Customer Support
food proof® SL GMO A2704-12 Soya Detection Kit
KIT230215 Kit for 50 reactions Store kit at -15 to -25 °C
For food testing purposes FOR IN VITRO USE ONLY
Hygiena® | Camarillo, CA 93012 USA |
diagnostics.support@hygiena.com |
www.hygiena.com
