hygiena KIT230001 Reagent D Vibrio Detection LyoKit User Guide

hygiena KIT230001 Reagent D Vibrio Detection LyoKit

Product Information

Specifications

• Product Name: Reagent D Ready Reference Guide
• Product Numbers: KIT230001, KIT230002, KIT230003
• Revision: A, November 2023
• Purpose: Rapid elimination of DNA from dead cells to avoid false-positive PCR results
• Contents:
  • KIT230003: 3 bottles containing 5 mL reagent each
  • KIT230001: 3 bottles containing 10 mL reagent each
  • KIT230002: 15 bottles containing 10 mL reagent each
• Hazard Level: Mutagenic potential, handle with caution

Product Usage Instructions

Before You Begin:
Reagent D has a mutagenic potential and can be hazardous. Follow these precautions:

• Always wear gloves when handling the substance.
• Use filter tips to avoid cross-contamination.
• Follow universal safety precautions for biohazardous and chemical materials.
• Dispose of contaminated materials properly and decontaminate work surfaces.
• Use a biosafety cabinet when aerosols might be generated.

Additional Equipment:
You will need the following additional equipment:

• foodproof D-Light LED lamp (Product No. MCH230039) or equivalent lamp with a 500 W halogen light bulb with cooling block for 1.5 ml reaction tubes.

Protocol:
Refer to the respective foodproof DNA isolation kit manual for detailed protocols. Use Reagent D as instructed in the specific protocols provided for different kits.

Available Protocols:

• foodproof StarPrep One Kit (KIT230175, KIT230176)
• foodproof StarPrep Two Kit (KIT230177)
• foodproof StarPrep Three Kit (KIT230187)
• foodproof StarPrep Six 8-Strip Kit (KIT230192)
• foodproof Magnetic Preparation Kit IV (KIT230184)

For specific product instructions, visit www.hygiena.com/documents.

Frequently Asked Questions (FAQ)

• Q: Is Reagent D safe to use without gloves?
  A: Reagent D has a mutagenic potential; therefore, it is highly recommended to always wear gloves when handling the substance to ensure safety.

• Q: Can Reagent D be stored at room temperature?
  A: It is advisable to store Reagent D according to the storage instructions provided on the product label to maintain its effectiveness.

Reagent D

Ready Reference Guide

Product No. KIT230001, KIT230002, KIT230003
Revision A, November 2023

Reagent D is designed for the rapid elimination of DNA from dead cells in enrichment cultures and samples to avoid false-positive PCR results. The reagent contains a light sensitive substance which can only penetrate the cell membranes of dead cells. Exposure to visible light leads to covalent binding of this substance to DNA and prevents the DNA from being amplified via PCR.
The reagent can be directly used in combination with the foodproof® DNA isolation kits.

KIT CONTENTS

• KIT230003: 3 bottles containing 5 mL reagent each
• KIT230001: 3 bottles containing 10 mL reagent each
• KIT230002: 15 bottles containing 10 mL reagent each

BEFORE YOU BEGIN

Reagent D has a mutagenic potential and the hazardousness has not been thoroughly investigated. Always wear gloves and handle this substance with caution.
In order to avoid cross-contamination use filter tips. Follow all universal safety precautions governing work with bio- and chemical-hazardous materials (e.g., wear lab coat and gloves at all times). Properly dispose all contaminated materials, decontaminate work surfaces and use a biosafety cabinet whenever aerosols might be generated.
Reaction tubes, deepwell plates or tube strips must have a transparent cap or cover. When writing on cap or cover, only use thin pencils to ensure light transfer through the cap into the tube or open cap carefully before exposure to light.

ADDITIONAL EQUIPMENT
foodproof D-Light LED lamp (Product No. MCH230039) or lamp with a 500 W halogen light bulb with cooling block for 1.5 ml reaction tubes
Please refer to the respective foodproof DNA isolation kit manual for a detailed list of instruments and reagents.

PROTOCOL

Please refer to the respective foodproof DNA isolation kit manual for a detailed protocol. The following protocols are available:

• foodproof StarPrep One Kit (Product No. KIT230175, KIT230176) or foodproof StarPrep One 8-Strip Kit (Product No. KIT230183):

  • Use Reagent D (KIT230001 or KIT230002)

• foodproof StarPrep Two Kit (Product No. KIT230177):

  • Product Instructions for Legionella
  • Product Instructions for Yeast & Mold
  • Product Instructions for Aspergillus
  • Product Instructions for Spoilage Yeasts
  • Use Reagent D (KIT230001 or KIT230002)

• foodproof StarPrep Two 8-Strip Kit (Product No. KIT230177):
  Product Instructions for Aspergillus

  • Use Reagent D (KIT230001 or KIT230002)

• foodproof StarPrep Three Kit (Product No. KIT230187):
  Product Instructions for Vibrio

  • Use Reagent D (KIT230003)

• Product Instructions for Enterobacteriaceae and Salmonella

  • Use Reagent D (KIT230001 or KIT230002)

• foodproof StarPrep Six 8-Strip Kit (Product No. KIT230192):
  Product Instructions for Yeast & Mold Quantification

  • Use Reagent D (KIT230001 or KIT230002)

• foodproof Magnetic Preparation Kit IV (Product No. KIT230184): Product Instructions for Salmonella, Enterobacteriaceae and Cronobacter

  • Use Reagent D (KIT230001 or KIT230002)

To find any Product Instructions, please visit www.hygiena.com/documents

Reagent D KIT230001 /02 /03
Store kit at -15 to -25 °C FOR IN VITRO USE ONLY
Made in Germany

Hygiena® | Camarillo, CA 93012 USA | diagnostics.support@hygiena.com | www.hygiena.com

References

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