hygiena KIT230042 Strip Kit User Guide
- June 17, 2024
- Hygiena
Table of Contents
hygiena KIT230042 Strip Kit User Guide
PCR kit for the qualitative detection of Escherichia coli O157 DNA using real-
time PCR instruments.
Before starting, it is strongly recommended to read the entire product manual
available on our website.
PROGRAM SETUP
Program your real-time PCR instrument before setting up the PCR reactions.
Select the following channels:
⇒ FAM (E. coli O157) and VIC (Internal Control).
As an alternative to VIC, HEX can be used. For the PikoReal® 24, Yakima Yellow
must be selected.
For some real-time PCR instruments the probe quencher as well as the use of a passive reference dye must be specified. This kit contains probes with TAMRA as quencher and no passive reference dye.
DATA INTERPRETATION
Verify results of positive (Control Template) and negative (H2O) controls,
before interpreting the sample results. Always compare samples to positive and
negative controls. Review data from each channel and interpret results
as described in the table.
foodproof® is a registered trademark of Hygiena. The above mentioned real-time PCR instruments are registered trademarks of their respective holders.
PREPARATION OF THE PCR MIX
Take appropriate precautions to prevent contamination, e.g., by using fi lter
tips and wearing gloves.
Thaw reagents, mix (do not vortex!) and briefl y spin vials before opening.
1. PREPARE PCR MIX
Add 18 µL of Master Mix (yellow cap), 1 µL of Enzyme Solution (red cap) and 1
µL of Internal Control (white cap) for each reaction to a suitable tube (n
samples + 2 controls + at least one additional reaction to cover pipetting
loss).
Mix carefully but thoroughly by pipetting up and down.
2. ADD PCR MIX
Pipet 20 µL of prepared PCR mix into each strip or plate well.
3. ADD SAMPLES AND CONTROLS
Pipet 5 µL of samples, Negative Control (colorless cap) or Control Template (purple cap) into respective wells.
4. SEAL
Seal strips/plate accurately.
5. CENTRIFUGE
Briefly spin strips/plate in a suitable centrifuge.
6. START REAL-TIME PCR RUN
Cycle samples as described above.
foodproof®
E. coli O157 Detection Kit
KIT230042
Kit for 96 reactions
Store kit at -15 to -25 °C
For food testing purposes
FOR IN VITRO USE ONLY
Made in Germany
Hygiena® | Camarillo, CA 93012 USA | diagnostics.support@hygiena.com | www.hygiena.com
References
Read User Manual Online (PDF format)
Read User Manual Online (PDF format) >>