hygiena KIT230060 Peanut Detection Kit User Guide

hygiena KIT230060 Peanut Detection Kit

Product Information

Specifications:

• Product Name: Peanut Detection Kit Ready Reference Guide
• Product No.: KIT230060
• Revision: A, December 2023
• Application: PCR kit for the qualitative detection of peanut DNA
• Usage: Real-time PCR instruments
• Kit Size: 64 reactions
• Intended Use: For food testing purposes

Product Usage Instructions

Program Setup:

1. Program your real-time PCR instrument with the following channels: FAM (peanut) and HEX (Internal Control).
2. Set the cycling parameters as follows:
3. 4 min at 95°C
4. 10 min at 60°C
5. 50 cycles of 5 sec at 95°C and 60 sec at 60°C with fluorescence detection

Data Interpretation:

1. Verify results of positive (Control Template) and negative controls (H2O) before interpreting sample results.
2. Always compare sample results to the positive and negative controls.
3. Interpret results based on the table provided in the manual.

Preparation of the PCR Mix:

1. Take precautions to prevent contamination by using filter tips and gloves.
2. Thaw reagents, mix without vortexing, and spin vials briefly before opening.
3. Add PCR mix to wells.
4. Add samples and controls.
5. Optionally, prepare a standard curve for quantification referring to the Allergen RM 800 product manual.
6. Seal strips/plates accurately.
7. Briefly spin strips/plate in a suitable centrifuge.
8. Start the real-time PCR run following the cycling parameters set up earlier.

FAQ

• Q: Can this kit be used for human DNA detection?
  A: No, this kit is specifically designed for the qualitative detection of peanut DNA and is not suitable for human DNA detection.

• Q: How should I store the kit when not in use?
  A: Store the kit at the recommended temperature indicated on the packaging. Avoid exposure to light and moisture.

Product No. KIT230060
PCR kit for the qualitative detection of peanut DNA using real-time PCR instruments.
Before starting, it is strongly recommended to read the entire product manual available on our website

PROGRAM SETUP

Program your real-time PCR instrument before setting up the PCR reactions. Select the following channels:

• FAM (peanut) and HEX (Internal Control).

For some real-time PCR instruments, the probe quencher as well as the use of a passive reference dye must be specified. This kit contains probes with a non- fluorescent “dark” quencher and no passive reference dye.

DATA INTERPRETATION

Verify results of positive (Control Template) and negative controls (H2O), before interpreting sample results. Always compare samples to positive and negative control. Review data from each channel and interpret results as described in the table.

PREPARATION OF THE PCR MIX

Take appropriate precautions to prevent contamination, e.g., by using filter tips and wearing gloves. Thaw reagents, mix (do not vortex!) and briefly spin vials before opening.

1. ADD PCR MIX Pipet 20 μL of Master Mix into each strip or plate well (n samples + 2 controls).

2. ADD SAMPLES AND CONTROLS Pipet 5 μL of samples, Negative Control (colorless cap) or Control Template (purple cap) into respective wells.
   OPTIONAL:
   STANDARD CURVEFor quantifi cation, prepare the standard curve. Please refer to the Allergen RM 800 (Product No. KIT230009) product manual.

3. SEAL Seal strips/plate accurately

4. CENTRIFUGE Briefly spin strips/plate in a suitable centrifuge.

5. START REAL-TIME PCR RUN Cycle samples as described above

foodproof® Peanut Detection Kit
KIT230060
Kit for 64 reactions
Store kit at -15 to -25 °C
For food testing purposes
FOR IN VITRO USE ONLY
Made in Germany
Hygiena® | Camarillo, CA 93012 USA | diagnostics.support@hygiena.com | www.hygiena.com

References

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