thermo scientific 13687714 Thermal Mixer User Manual

thermo scientific 13687714 Thermal Mixer

This manual covers the following ThermoScientific Thermal Mixers and parts for them, by the catalog number:

• 13687711 – Thermal Mixer (US/UK/EU plug)
• 13687722 – Thermal Mixer (AUS/CHN plug)
• 13687712 – Thermoblock for 24 x 1.5 ml microtubes (US/UK/EU plug)
• 13687713 – Thermoblock for 24 × 2.0 ml microtubes (US/UK/EU plug)
• 13687714 – Thermoblock for 20 x 0.2 ml & 12 x 1.5 ml tubes (US/UK/EU plug)
• 13687715 – Thermoblock for 20 x 0.5 ml & 12 x 1.5 ml tubes (US/UK/EU plug)
• 13687716 – Thermoblock for 96-well PCR microplate or 0.2 ml tubes/strips (US/UK/EU plug)
• 13687717 – Thermal Mixer with thermoblock for 24 × 1.5 ml microtubes (US/UK/EU plug)
• 13687718 – Thermal Mixer with thermoblock for 24 x 2.0 ml microtubes (US/UK/EU plug)
• 13687719 – Thermal Mixer with thermoblock for 20 x 0.2 ml & 12 x 1.5 ml tubes (US/UK/EU plug)
• 13687720 – Thermal Mixer with thermoblock for 20 x 0.5 ml & 12 x 1.5 ml tubes (US/UK/EU plug)
• 13687721 – Thermal Mixer with thermoblock for 96-well PC microplate or 0.2 ml tubes/strips (US/UK/EU plug)

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Our Sales Support staff can provide information on pricing and give you quotations. We can take your order and provide delivery information on major equipment items or arrange to have your local sales representative contact you. Our products are listed on the Internet and we can be contacted through our Internet home page. Our Service Support staff can supply technical information about proper setup, operation, or troubleshooting of your equipment. We can fill your needs for spare or replacement parts or provide you with on-site service. We can also provide you with a quotation on our Extended Warranty for your Thermo Scientific products. Whatever Thermo Scientific products you need or use, we will be happy to discuss your applications. If you are experiencing technical problems, working together, we will help you locate the problem and, chances are, correct it yourself … over the telephone without a service call. When service that is more extensive is necessary, we will assist you with direct factory-trained technicians or a qualified service organization for on-the-spot repair. If your service need is covered by the warranty, we will arrange for the unit to be repaired at our expense and to your satisfaction. Regardless of your needs, our professional telephone technicians are available to assist you Monday through Friday from 8:00 a.m. to 6:00 p.m. Eastern Time. Please contact us by telephone or fax. If you wish to write, our mailing address is:

• Thermo Fisher Scientific
• 401 Millcreek Road, Box 649
• Marietta, OH 45750

International customers, please contact your local Thermo Scientific distributor.

Introduction

This Thermo Scientific Thermal Mixer provides mixing and temperature control of samples in micro test tubes or PCR plates. Features of thermal mixers meet the highest expectations of users according to many parameters:

• Fast reaching of specified mixing speed and maintenance of equal amplitude of rotation throughout the thermal mixer block;
• The stability of maintaining the set temperature in a wide range throughout the block surface of thermal mixers;
• With the help of the temperature calibration function, The user can calibrate the unit approximately ±6% of the selected temperature to compensate for differences in the thermal behavior of tubes from different manufacturers;
• LCD display indicates pre-set and current values of temperature, speed and time of operation;
• Quiet motor operation, compact size, prolonged service life;
• Sensor error handling and diagnostics;

Functions of heating and mixing can be performed either simultaneously or independently, that allows using the unit as three independent devices:

• Thermostat;
• Shaker;
• Thermo-shaker.

We offer five heating and cooling blocks for each model, including a block with a plastic lid for PCR-plates. Within one model of the thermal mixer, the blocks are mutually interchangeable and can be easily installed.

The devices are applicable in:

• Genetic analyses – in the extraction of DNA, RNA and further sample preparation;
• Biochemistry – for studying of enzymatic reactions and processes;
• Cellular biology – extraction of metabolites from cellular material.

Safety Information

Caution! Make sure you have fully read and understood the present Manual before using the equipment. Please pay special attention to the sections marked by this symbol.
Caution! Hot surface! The platform surface becomes very hot during use. Always use protective cotton gloves to install or remove samples when the temperature is set higher than 60°C.

General safety

• Use only as specified in the operating manual provided.
• Save the unit from shocks or falling.
• Store and transport the unit in a horizontal position (see package label) at ambient temperatures between -20°C and +60°C and maximum relative humidity of 80%.
• After transportation or storage keep the unit under room temperature for 2-3 h before connecting it to the electric circuit.
• Use only original parts and accessories, provided by Thermo Fisher Scientific for this product.
• Before using any cleaning or decontamination methods except those recommended by Thermo Fisher Scientific, check with Thermo Fisher Scientific that the proposed method will not damage the equipment.
• Do not make modifications to the design of the unit.

Electrical safety

• Connect only to the external power supply with a voltage corresponding to that on the serial number label.
• Use only the external power supply provided with this product.
• Ensure that the power switch and external power supply are accessible during use.
• Do not plug the unit into an ungrounded power socket, and do not use an ungrounded extension lead.
• Disconnect the unit from the electric circuit before moving.
• If the liquid penetrates into the unit, disconnect it from the external power supply and have it checked by a repair and maintenance technician.
• Do not operate the unit in premises where condensation can form. The operating conditions of the unit are defined in the Specifications section.

During operation

• Do not leave the operating unit unattended.
• Do not impede the platform motion.
• Do not operate the unit in environments with aggressive or explosive chemical mixtures. Please contact Thermo Fisher Scientific for possible operation of the unit in specific atmospheres.
• Do not operate the unit if it is faulty or has been installed incorrectly.
• Do not use outside laboratory rooms.
• Do not check the temperature by touch. Use a thermometer.

Biological safety

• It is the user’s responsibility to carry out appropriate decontamination if hazardous material is spilled on or penetrates into the equipment.

Getting Started

Unpacking
Remove packing materials carefully and retain them for future shipment or storage of the unit. Examine the unit carefully for any damage incurred during t transit. The warranty does not cover in-transit damage. The warranty covers only units transported in the original package.

Setup
Place the unit upon an even horizontal stable non-flammable surface 30 cm away from any flammable materials, and clear 20 cm around the device on all sides for ventilation. Remove the protective film from the display. Plug the external power supply into the socket at the rear side of the unit. Connect the power cable to the external power supply.

Thermoblock installation
If a thermoblock is not installed, choose the thermoblock, and connect the plug to the contact terminal according to the scheme on Fig. 1/1 on the underside of the thermoblock. Make sure that the connector is mounted tightly.

• Caution! Thermoblock installation and replacement have to be performed only when the Power switch is turned off and the external power supply is disconnected from the device.

Align the thermo block so that the warning label is facing the front of the unit (fig. 2). Secure with the four knurled screws (fig. 2/1) or four hex screws.

Changing blocks
Disconnect the external power supply from the device. Remove the four knurled screws or four hex screws (in microplate thermoblocks). Lift the block without damaging the cable and disconnect the plug (fig. 1 /1 ). Select the new thermoblock and install it according to the previous paragraph .

Operation

Recommendations during operation

• Please check the tubes/microplates before using, be sure that tubes and microplates are heat-resistant.
• Do not heat the microplates over the melting point of the material they are made of.
• We recommend filling tubes and plate wells up to 75% of the rated volume for efficiency.

Caution! The platform surface becomes very hot during use. Please, take necessary care and use protective cotton gloves to install or remove test samples when set temperature is higher than 60°C.

• Caution! Do not fill microtubes or microplates directly inside the unit.
• Note! If the rotation speed is set to zero, pressing the rpm Run/Stop key will start the timer but the platform will not move.

Starting the unit
Connect the external power supply to a grounded power socket and set the power switch, located on the rear panel of the unit, to position I (on). The display will turn on with the upper line (Set) showing the time, speed, and temperature set earlier and the lower line (Actual) showing the current status: STOP indication, 000 rpm speed, and platform temperature in °C. If a temperature is set, then the platform temperature automatically rises to that temperature. The time of temperature stabilization depends on the room temperature. If the heating is turned off by setting the temperature below or 4 °C, the top line shows an indication of OFF.

Setting the parameters
Use the readings in the upper line of the display (Set), while setting the required parameters. Pressing the key for more than 3 seconds will increase the increment rate. Speed and temperature can be changed during operation. Using the Time keys (fig. 3/1) set the required working time interval in hours and minutes (step 1 min). Using the Speed keys (fig. 3/2) set the required speed (step 10 rpm). Using the Temp keys (fig. 3/3) set the necessary temperature (step 0.1 °C).

Program execution
After the thermal stabilization of the thermal mixer, i.e. when the set and current temperature readings become the same: Place samples on the platform. Press the rpm Run/Stop key (fig. 3/ 4). The platform will start rotating and the timer indicator will start counting the time interval (with 1 min precision). After finishing the program (after the set t ime elapses) the platform motion will stop and the timer will show the flashing reading STOP accompanied by the repetitive sound signal until the rpm Run/Stop key is pressed. If the working time is not set (or is reset) and the timer indicator in the upper line shows 00:00, pressing the rpm Run/Stop key will start the continuous operation of the device with countdown timer in the lower line (Actual) until the rpm Run/Stop key is pressed again.

Operation

Caution! At the end of the set time period, the platform movement is stopped automatically, but the heating can be stopped only manually by reducing the down temperature using the Temp key (fig. 3/3 – lower key) till the OFF sign appears in the upper line (Set) of the display Caution! The platform remains hot after use. Please, take necessary care and use protective cotton gloves to install or remove test samples when a set temperature is higher than 60°C.

If required, there is the possibility to restart the timer when it is running. Press the Time Run/Stop key once (fig. 3/5) to stop the timer. Press the Time Run/Stop key again to restart the timer. The platform motion can be stopped at any time by pressing the rpm Run/Stop key. In this case, the program realization and the platform motion will stop and the timer will switch into the STOP mode saving previously set time. Press the rpm Run/Stop key to repeat the operation with the same time and speed.

Finishing the operation
Set the Power switch, located on the rear panel of the unit, in position O (Off) and disconnect the external power supply from the electric circuit.

Calibration

Note. Values marked in grey on figures 4 and 5 are not used in calibration and are meant for service engineers.

Figure 4. Display in calibration mode:

1. Calibration mode indicator;
2. Calibration coefficient;
3. Temperature with current coefficient

• Note. Coefficient value changes are recommended after the unit has reached 30°C temperature.
• Note. Calibration coefficient can be changed in range from 0.936 to 1.063 (±0.063), with an increment of 0.001. This calibrating coefficient will correct temperature through all the operation ranges.

Figure 5. Changing the coefficient:

1. Calibration coefficient;
2. Temperature with current coefficient

Figure 6. Display after calibration:

1. Set temperature;
2. Current calibrated temperature

The device is precalibrated at the factory (calibrating coefficient is 1 .000) for operation with temperatures measured by a sensor in the heating block. To change the calibration coefficient, hold the Time Run/Stop key pressed for more than 8 s to activate calibration mode. The calibration coefficient appears on the display (figure 4).

Restoring factory settings
Set 1.000 value using the up and down Temp keys as shown on fig. 4/1 to restore the factory settings. Press the rpm Run/Stop key once to save the changes and exit the calibration mode.

Calibration procedure
To calibrate the unit, use an independent sensor with 0.5°C accuracy, which can fit in the cell of a microplate on the platform. Install the sensor into a cell of the microplate. Set the required temperature in operation mode, e.g. 40°C. After the unit reaches the set temperature (when the set and current temperature readings are equal), leave the unit for 30 min for thermal stabilization. Let us assume that the readings of the independent sensor is 39°C, but the display’s actual temperature is 40°C. Then, it is necessary to add a 1 °C correction. Hold the Time Run/Stop key pressed for more than 8 s to activate calibration mode (Figure 4). Using the up and down Temp keys, change the calibration coefficient (fig. 5/1) so that the new temperature value (fig. 5/2) corresponds to the independent sensor temperature. In our example, the calibration coefficient will be 0.974. Press the rp Run/Stop key once to save the changes and exit the calibration. The display will show the calibrated temperature as shown in Fig. 6/1 and the unit will continue thermal stabilization according to the previously set temperature.

Specifications

The unit is designed for operation in cold rooms, incubators (excluding CO2 incubators) and closed laboratory rooms at ambient temperature from +4°C to +40°C in a non-condensing atmosphere and maximum relative humidity 80% for temperatures up to 31 °C decreasing linearly to 50% relative humidity at 40°C. Thermo Fisher Scientific is committed to a continuous program of improvement and reserves the right to alter the design and specifications of the equipment without additional notice.

Table 1. Thermoblocks

Table 2. Replacement part

1. Room temperature
2. Data for 75% filled tubes or microplates
3. The recommended interval between prolonged operation sessions not less than 1 hour
4. Accurate within ±10%

Maintenance

Service
It the unit requires service maintenance. disconnect the unit from the electric circuit c:1nd contact Thermo Fisher Scientific. All maintenance and repair operations must be performed only by qualified and specially trained personnel.

Cleaning
Standard ethanol (75%) or other cleaning agents recommended for cleaning of laboratory equipment can be used for cleaning and decontamination of the unit.

Rubber belt replacement
For maintenance of reliable operation of tho device, Thermo Fisher Scientific recommends replacing rubber belts after 1.5 years or 2000 hours of operation time. Disconnect the tl1e external p0vver supply from the tl1e device. Remove 4 fixation screws on the device bottom und remove tho bottom plate. Replace the rubber bell (fig. 7). Reassemble the device,

Error codes in case of a defect
Some mHlfunctions trigger an error code to appear on display, accompanied by a sound signal every 8 s. Press rpm Run/Stop key to turn off the signal. The error code format is letters ER and a single digit. Disconnect the tl1e unit from the electric circuit and report the error code to Tl1ermo Fisher Scientific.

Warranty

The following information will be required in the event that warranty or post- warranty service becomes necessary. Complete the table below and retain for your records.

Thermo Fisher Scientific guarantees the compliance of the unit with the requirements of specifications if the customer follows operation, storage and transportation instructions. The warranted service life of unit from date of delivery to the customer is 24 months, excluding blocks and replacement parts mentioned in Tables 1 & 2. For an extended warranty, contact Thermo Fisher Scientific. The warranty covers only the units transported in the original package. If any manufacturing defects are discovered by the Customer, an unsatisfactory equipment report shall be compiled, certified, and sent to Thermo Fisher Scientific.

EU Declaration of Conformity

This declaration of conformity indicates that the equipment meets the requirements of the following Directive(s):

• EMC Directive 2014/30/EU
• Low Voltage Directive 2014/35/EU
• RoHS2 2011/65/EU1
• WEEE 2012/19/EU

Applied standards:

• EN 61326-1
  • Electrical equipment for measurement, control, and laboratory use EMC requirements. General requirements.
• EN 61010-1
  • Safety requirements for electrical equipment for measurement, control, and laboratory use. General requirements.
• EN 61010-2-010
  • Particular requirements for laboratory equipment for the heating of materials.
• EN 61010-2-051
  • Particular requirements for laboratory equipment for mixing and stirring.

Find out more at thermofisher.com For Research Use Only. Not for use in diagnostic procedures. © 2019 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified.

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