amino DNA Extraction Kit Extract DNA from Strawberry Fruit Instructions
- June 10, 2024
- amino
Table of Contents
DNA Extraction Kit Extract DNA from Strawberry Fruit
Instructions
DNA Extraction Kit Extract DNA from Strawberry Fruit
Kit Contents
(1) 10 mL tube sterile distilled water
(1) 1.5 mL tube lysis buffer
(1) Ziplock-style ‘mixing‘ bag
(1) filter
(1) 0.3 g packet of electrolyte (salt)
(1)10 mL tube to measure & hold isopropyl alcohol (91% + )
You Provide
(1) strawberry, fresh or frozen*
(1) plastic or glass cup/mug ~9 mL isopropyl alcohol (91% or above is best,
but 70% can work, especially if you use it cold)
-
if you have a strawberry allergy, or do not have any, you can use a piece of kiwifruit or banana with similar results.
Remember! Wearing gloves when doing any science experiments is good practice. Have a look at our Safe Science guidelines for more safety information: www.amino.bio/safe-science- Add the sterile distilled water and electrolyte (salt) to the mixing bad
- Find a nice strawberry and add it to the bag
- Mash up the strawberry into a slurry by pressing the bag
- Add the lysis buffer to the strawberry mash and mix
- Place the filter in the a clean cup
- Pour the strawberry mix into the filter
- Wait 5 minutes to let liquid pass through the filter
- Add ~ 9 mL of isopropyl alcohol to the tube provided
- Pour a small amount of filtered liquid into the isopropyl alcohol
- Over the next 5 minutes, watch the DNA precipitate and float up to the surface!
- Add the sterile distilled water and electrolyte (salt) to the mixing bad
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