dynamic BIOSENSORS AS-2-Gb-lfs Adapter Strand User Manual
- September 27, 2024
- dynamic BIOSENSORS
Table of Contents
dynamic BIOSENSORS AS-2-Gb-lfs Adapter Strand
Key Features
- Adapter strand 1 – Gc for functionalization of helix ® Adapter Chip Spot 1.
- Compatible with helix ® Adapter Chip.
- Includes Adapter strands for 50 regenerations.
- Ideal for MIX&RUN sample preparation.
- Adapter strand 1 carries a hydrophobic green dye (Gc) with a neutral net charge.
Adapter Chip Overview
2 spots with 2 different anchor sequences for DNA-encoded addressing.
Product Description
Order Number: AS-1-Gc
Table 1. Contents and Storage Information
Material| Cap| Concentration| Amount| Buffer|
Storage
---|---|---|---|---|---
Adapter strand 1 – Gc| Black| 400 NM| 5 x 100 µL| TE40 [1]| -20°C
For research use only.
This product has a limited shelf life, please see expiry date on label.
To avoid many freeze thaw cycles please aliquot the nanolayer.
Preparation | MIX&RUN
n-solution hybridization of adapter and ligand strands:
- Mix Adapter strand 1 – Gc (400 NM) and conjugated Ligand strand (500 NM) at 1:1 ratio (v/v).
- Incubate the solution of step 1 at RT at 600 rpm for 30 min to ensure complete hybridization.
- Mix solution of step 2 and Adapter strand 1 – Gc – lfs (200 nM) at 1:1 ratio (v/v).
Solution is ready to use for biochip functionalization.
Stability of the solution is related to the stability of the ligand molecule.
Table 2. Additional material for functionalization of spot 1 and reference spot 2.
Material| Concentration| Buffer| Related Product Name|
Order No
---|---|---|---|---
Ligand strand carrying the conjugated ligand| 500 NM| PE40 [| helix ® Amine
Coupling Kit| HK-NHS-1
Adapter strand 1 – Gc – lbs.| 200/250 NM| TE40 [1]| Adapter strand 2 with
green dye Gc prehybridized with ligand-free strand| AS-2-Gb-lfs
Example
Required volume for 3 functionalization’s: 100 µL with a final concentration of 100 nM.
| Vial 1 | Vial 2 |
|---|
Adapter strand 1 – Gc
(400 NM)| Conjugated Ligand strand (500 NM)| Adapter strand 1 – Gc – lfs
(200/250 NM)
25 µ| 25 µL| 50 µL
After incubation time, mix vial 1 and vial 2 to obtain 100 µL of ready-to-use DNA solution.
Customer Support
Contact
Dynamic Biosensors GmbH
Perchtinger Str. 8/10
81379 Munich
Germany
Dynamic Biosensors, Inc.
300 Trade Center, Suite 1400
Woburn, MA 01801
USA
Order Information order@dynamic-biosensors.com
Technical Support support@dynamic-biosensors.com
www.dynamic-biosensors.com
Instruments and chips are engineered and manufactured in Germany. ©2024
Dynamic Biosensors GmbH | Dynamic Biosensors, Inc. All rights reserved.
References
Read User Manual Online (PDF format)
Read User Manual Online (PDF format) >>