dynamic BIOSENSORS AS-2-Ra v5.1 Adapter Strand User Manual
- September 27, 2024
- dynamic BIOSENSORS
Table of Contents
dynamic BIOSENSORS AS-2-Ra v5.1 Adapter Strand
Product Information
Specifications:
- Product Name: heliX+ ADAPTER STRAND 2 with red dye Ra
- Manufacturer: Dynamic Biosensors GmbH & Inc.
- Order Number: AS-2-Ra
- Key Features: 2 spots with 2 different anchor sequences for DNA-encoded addressing
Product Description:
This product contains Adapter strand 2 – Ra with a concentration of 400 nM.
It is for research use only and has a limited shelf life. Please see the
expiry date on the label. To avoid multiple freeze-thaw cycles, it is
recommended to aliquot the nanolever.
Preparation
- Mix Adapter strand 1 – Ra (400 nM) and conjugated Ligand strand with ligand 1 (500 nM) at a 1:1 ratio (v/v).
- Mix Adapter strand 2 – Ra (400 nM) and conjugated Ligand strand with ligand 2 (500 nM) at a 1:1 ratio (v/v).
- Incubate separately the two solutions from steps 1 and 2 at room temperature at 600 rpm for 30 minutes to ensure complete hybridization.
- Mix the solutions from steps 1 and 2 at a 1:1 ratio (v/v). The solution is now ready to use for biochip functionalization.
The stability of the solution is dependent on the stability of the ligand molecules.
FAQ:
-
Q: Can I use this product for clinical purposes?
A: No, this product is for research use only. -
Q: How should I store the product?
A: Store as per the storage information provided on the label. Avoid multiple freeze-thaw cycles. www.dynamic-biosensors.com
Key Features
- Adapter strand 2 for functionalization of heliX® Adapter Chip Spot 2.
- Compatible with heliX® Adapter Chip.
- Includes Adapter strands for 50 regenerations.
- Ideal for MIX&RUN sample preparation.
- Adapter strand 2 carries a moderately hydrophilic red dye (Ra) with a single positive net charge.
heliX® Adapter Chip Overview
2 spots with 2 different anchor sequences for DNA-encoded addressing.
Product Description
Order Number: AS-2-Ra
Table 1. Contents and Storage Information
| Material | Cap | Concentration | Amount | Buffer | Storage |
|---|---|---|---|---|---|
| Adapter strand 2 – Ra | White | 400 nM | 5 x 100 µL | TE40 [ 1] |
-20°C
For research use only.
This product has a limited shelf life, please see expiry date on label.
To avoid many freeze thaw cycles please aliquot the nanolever.
Preparation | MIX&RUN
In-solution hybridization of adapter and ligand strands:
- Mix Adapter strand 1 – Ra (400 nM) and conjugated Ligand strand with ligand 1 (500 nM) at 1:1 ratio (v/v).
- Mix Adapter strand 2 – Ra (400 nM) and conjugated Ligand strand with ligand 2 (500 nM) at 1:1 ratio (v/v).
- Incubate separately the two solutions of step 1 and 2 at RT at 600 rpm for 30 min to ensure complete hybridization.
- Mix solution of step 1 and 2 at 1:1 ratio (v/v).
Solution is ready to use for biochip functionalization.
Stability of the solution is related to the stability of the ligand molecules.
Table 2. Additional material for functionalization of spot 1 and reference spot 2.
| Material | Concentration | Buffer | Related Product Name | Order No |
|---|---|---|---|---|
| Adapter strand 1 – Ra | 400 nM | TE40 [ 1 ] | ** _Adapter strand | |
| 1_ with red dye Ra** | AS-1-Ra | |||
| Ligand strand carrying the conjugated ligand 1 | 500 nM | P 2 | ||
| ] E40 [ | heli X ® Amine Coupling Kit 1 | HK-NHS-1 | ||
| Ligand strand carrying the conjugated ligand 2 | 500 nM | PE40 [ | ||
| 2 ] | heli X ® Amine Coupling Kit 1 | HK-NHS-1 |
Example
Required volume for 3 functionalizations: 100 μL with a final concentration of
100 nM.
| Vial 1 | Vial 2 |
|---|---|
| Adapter strand 1 – Ra (400 nM) | Conjugated Ligand strand with |
| ligand 1 (500 nM) | Adapter strand 2 – Ra (400 nM) |
Ligand strand + with ligand 2 (500 nM)
25 µL| 25 µL| 25 µL| 25 µL
After incubation time, mix vial 1 and vial 2 to obtain 100 μL of ready-to-use DNA solution.
Contact
Dynamic Biosensors GmbH
Perchtinger Str. 8/10
81379 Munich
Germany
Dynamic Biosensors, Inc.
300 Trade Center, Suite 1400
Woburn, MA 01801
USA
Order Information order@dynamic-biosensors.com
Technical Support support@dynamic-biosensors.com
www.dynamic-biosensors.com
Instruments and chips are engineered and manufactured in Germany.
©2024 Dynamic Biosensors GmbH | Dynamic Biosensors, Inc. All rights reserved.
TE40: 10 mM Tris, 40 mM NaCl, 0.05 % Tween20, 50 μM EDTA, 50 μM EGTA
If the protein is not stable in PE40 (TE40, HE40), please check buffer
compatibility with the switchSENSE® compatibility sheet.
www.dynamic-biosensors.com
References
Read User Manual Online (PDF format)
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