dynamic BIOSENSORS HK-SXT-1 Twin-Strep Tag Capture Kit User Manual

: September 17, 2024
: dynamic BIOSENSORS

Table of Contents

dynamic BIOSENSORS HK-SXT-1 Twin-Strep Tag Capture Kit
Product Information
heliX® Adapter Chip Overview
Product Description
Workflow of a heliX® SXT – capture assay
References
Read User Manual Online (PDF format)
Download This Manual (PDF format)

dynamic BIOSENSORS HK-SXT-1 Twin-Strep Tag Capture Kit

Product Information

Specifications

Product Name: heliX+ TWIN-STREP-TAG CAPTURE KIT with red dye Ra
Manufacturer: Dynamic Biosensors GmbH & Inc.
Model: HK-SXT-1 v6.1
Key Features:
- 2 spots with 2 different anchor sequences for DNA-encoded addressing.
- Spot 1 is functionalized with the capture molecule while Spot 2 is used as real-time reference.
Order Number: HK-SXT-1
For research use only.
Shelf Life: Limited, 3 months after preparation of ready-to-use solution

Product Description
The heliX+ TWIN-STREP-TAG CAPTURE KIT with red dye Ra is designed for capturing ligands of interest on the surface during measurement runs. It allows for the measurement of analyte binding kinetics or conformational changes upon analyte binding. The kit includes ligand and adapter strands with specific concentrations and storage information as detailed in Table 1.

Preparation

Assay Setup in heliOS

Go to heliOS > create a New Assay Workflow > add Custom Assay > load Capture with Kinetics.
Modify the parameters based on your needs and run the assay.

Suggested assay parameters (e.g., flow rate, time, LED power, etc.) are within the heliOS assay.

For further questions, please contact the support team at support@dynamic- biosensors.com

Useful Order Numbers

Chip with 2 detection spots: ADP-48-2-0
For passivation of chip surface: SOL-PAS-1-5
For regeneration of chip surface: SOL-REG-1-5

Contact Information

Dynamic Biosensors GmbH
Perchtinger Str. 8/10
81379 Munich Germany

Dynamic Biosensors, Inc.
300 Trade Center, Suite 1400
Woburn, MA 01801 USA

Order Information
order@dynamic-biosensors.co

Technical Support
support@dynamic-biosensors.com

Frequently Asked Questions (FAQ)

Q: What is the shelf life of the heliX+ TWIN-STREP-TAG CAPTURE KIT?
- A: The kit has a limited shelf life. Please see the expiry date on the label. After preparation of the ready-to-use solution, the expiry date is 3 months.
Q: How can I regenerate the chip surface?
- A: Surface regeneration can be done by injection of a high pH solution. This step helps the chip surface go back to its original state.

heliX+
User Manual

TWIN-STREP-TAG CAPTURE KIT with red dye Ra
Dynamic Biosensors GmbH & Inc.
HK-SXT-1 v6.1

Key Features

This kit is designed for capture of Twin-Strep-tagged molecules using Strep-Tactin®XT (SXT).
Compatible with heliX® Adapter Chip.
Includes Adapter strands and Ligand strand modified with Strep-Tactin®XT for 20 regenerations.
For functionalization of Spot 1 and Spot 2.
Adapter strands 1 and 2 carry a moderately hydrophilic red dye (Ra) with a single positive net charge.

heliX® Adapter Chip Overview

2 spots with 2 different anchor sequences for DNA-encoded addressing. Spot 1 is functionalized with the capture molecule while Spot 2 is used as real-time reference.

Product Description

Order Number: HK-SXT-1
Table 1. Contents and Storage Information

Material	Cap	Concentration	Amount	Buffer
SXT [ 1 ] – Ligand strand	Green	500 nM	2 x 100
µL	TE40 [ 2]	2-8°C
*Adapter strand 1 – Ra*	Black	400 nM	2 x 100 µL	TE40 [ 2]

-20°C
Adapter strand 2 – Ra – lfs| White| 200/250 nM| 2 x 200 µL| TE40 [ 2]| -20°C

For research use only.

This product has a limited shelf life, please see expiry date on label. After preparation of ready to use solution the expiry date is 3 months.

Workflow of a heliX® SXT – capture assay

The anchor strand (ssDNA) immobilized to the surface of the heliX® Adapter Chip is hybridized with complementary DNA strands modified with Strep-Tactin®XT.
The twin-strep-tagged ligand of interest is captured on the surface during the measurement run.
Measurement of the analyte binding kinetics or conformational change upon analyte binding.
Surface regeneration by injection of a high pH solution. Chip surface goes back to the original state. This step can be followed by a new hyridization of fresh ligand with Strep-Tactin®XT.

Preparation

Mix 100 µL SXT – Ligand strand with 100 µL Adapter strand 1 – Ra.
Incubate the solution of step 1 at RT at 600 rpm for 30 min to ensure complete hybridization.
Mix 200 µL Adapter strand 2 – Ra – lfs to the sample after step 2.

The solution (400 µL in total) is ready to use for a biochip functionalization.
Please aliquot and store the ready to use solution at 2-8°C. Use up within 3 months.
The kit contains material for the preparation of two separate ready to use solutions with 400 µL each.

Assay Setup in heliOS

Go to heliOS > create a New Assay Workflow > add Custom Assay > load Capture with Kinetics > modify the parameters based on your needs and run the assay.
Suggested assay parameters (e.g., flow rate, time, LED power, etc.) are within the heliOS assay.
For further questions, please contact the support team at support@dynamic-biosensors.com

Useful Order Numbers
Table 2. Order Numbers