dynamic BIOSENSORS ASP-1-Ra Adapter Strand Package User Manual
- September 9, 2024
- dynamic BIOSENSORS
Table of Contents
ASP-1-Ra Adapter Strand Package
Specifications:
-
Product Name: heliX+ ADAPTER STRAND PACKAGE with red dye
Ra -
Manufacturer: Dynamic Biosensors GmbH & Inc.
-
Model: ASP-1-Ra v5.1
-
Key Features: 2 spots with 2 different anchor sequences for
DNA-encoded addressing
Product Description:
The heliX+ ADAPTER STRAND PACKAGE with red dye Ra (ASP-1-Ra)
includes Adapter strand 1 – Ra, Adapter strand 2 – Ra – lfs, and
other necessary materials for biochip functionalization.
Contents and Storage Information:
| Material | Cap Color | Concentration |
|---|---|---|
| Adapter strand 1 – Ra | Black | 400 nM |
| Adapter strand 2 – Ra – lfs | White | 200/250 nM |
Product Usage Instructions:
Preparation | MIX&RUN:
-
Mix Adapter strand 1 – Ra (400 nM) and conjugated Ligand strand
(500 nM) at a 1:1 ratio (v/v). -
Incubate the solution from step 1 at room temperature at 600
rpm for 30 minutes to ensure complete hybridization. -
Mix the solution from step 2 and Adapter strand 2 – Ra – lfs
(200 nM) at a 1:1 ratio (v/v). -
The solution is now ready to use for biochip
functionalization.
Note: Stability of the solution is related to the stability
of the ligand molecule.
FAQ:
Q: Can the heliX+ ADAPTER STRAND PACKAGE be used for clinical
purposes?
A: No, this product is for research use only.
Q: How should I store the heliX+ ADAPTER STRAND PACKAGE to
maximize shelf life?
A: Store as per the storage information provided and avoid
multiple freeze-thaw cycles by aliquoting the nanolever.
heliX+
User Manual
ADAPTER STRAND PACKAGE
with red dye Ra
Dynamic Biosensors GmbH & Inc. ASP-1-Ra v5.1
Key Features
· Adapter strand 1 – Ra and Adapter strand 2 – Ra – lfs (ligand free strand)
for functionalization of heliX® Adapter Chip on Spot 1 and Spot 2,
respectively.
· Compatible with heliX® Adapter Chip. · Includes Adapter strands for 50
regenerations. · Ideal for MIX&RUN sample preparation. · Adapter strands 1 and
2 carry a moderately hydrophilic red dye (Ra) with a single positive net
charge.
heliX® Adapter Chip Overview
2 spots with 2 different anchor sequences for DNA-encoded addressing.
Product Description
Order Number: ASP-1-Ra
Table 1. Contents and Storage Information Material Adapter strand 1 – Ra Adapter strand 2 – Ra – lfs
Cap Black White
Concentration 400 nM 200/250 nM
Amount 5 x 100 µL 5 x 200 µL
For research use only. This product has a limited shelf life, please see expiry date on label. To avoid many freeze thaw cycles please aliquot the nanolever.
Buffer TE40 [1] TE40 [1]
Storage -20°C -20°C
ASP-1-Ra v5.1
www.dynamic-biosensors.com
Page 1 of 3
Preparation | MIX&RUN
In-solution hybridization of adapter and ligand strands:
1. Mix Adapter strand 1 – Ra (400 nM) and conjugated Ligand strand (500 nM)
at 1:1 ratio (v/v).
2. Incubate the solution of step 1 at RT at 600 rpm for 30 min to ensure
complete hybridization.
3. Mix solution of step 2 and Adapter strand 2 – Ra – lfs (200 nM) at 1:1
ratio (v/v).
Solution is ready to use for biochip functionalization.
Stability of the solution is related to the stability of the ligand molecule.
Table 2. Additional material for functionalization of spot 1 and reference spot 2.
Material
Ligand strand carrying the conjugated ligand
Concentration 500 nM
Buffer P2]E40 [
Related Product Name heliX® Amine Coupling Kit 1
Order No HK-NHS-1
Example
Required volume for 3 functionalizations: 100 µL with a final concentration of 100 nM.
Vial 1
Adapter strand 1 – Ra (400 nM)
25 µL
Conjugated Ligand strand (500 nM)
25 µL
Vial 2
Adapter strand 2 – Ra – lfs (200/250 nM)
50 µL
After incubation time, mix vial 1 and vial 2 to obtain 100 µL of ready-to-use DNA solution.
ASP-1-Ra v5.1
www.dynamic-biosensors.com
Page 2 of 3
Contact
Dynamic Biosensors GmbH Perchtinger Str. 8/10 81379 Munich Germany
Dynamic Biosensors, Inc. 300 Trade Center, Suite 1400 Woburn, MA 01801 USA
Order Information order@dynamic-biosensors.com Technical Support support @dynamic-biosensors.com
www.dynamic-biosensors.com
Instruments and chips are engineered and manufactured in Germany. ©2024
Dynamic Biosensors GmbH | Dynamic Biosensors, Inc. All rights reserved.
[1] TE40: 10 mM Tris, 40 mM NaCl, 0.05 % Tween20, 50 M EDTA, 50 M EGTA [2] If the protein is not stable in PE40 (TE40, HE40), please check buffer compatibility with the switchSENSE® compatibility sheet.
ASP-1-Ra v5.1
www.dynamic-biosensors.com
Page 3 of 3
References
Read User Manual Online (PDF format)
Read User Manual Online (PDF format) >>