dynamic BIOSENSORS AS-2-Rb-lfs Adapter Strand 2 User Manual
- September 6, 2024
- dynamic BIOSENSORS
Table of Contents
**dynamic BIOSENSORS AS-2-Rb-lfs Adapter Strand 2 User Manual
**
ADAPTER STRAND 2
with red dye Rb and prehybridized with ligand strand Dynamic Biosensors
GmbH & Inc.
AS-2-Rb-lfs v5.1
Key Features
- Adapter strand 2 for functionalization of heliX® Adapter Chip Spot 2.
- Compatible with heliX ® Adapter Chip.
- Includes Adapter strands for 50 regenerations.
- Ideal for MIX &RUN sample preparation.
- Adapter strand 2 carries a hydrophilic red dye (Rb) with a neutral net charge.
heliX ® Adapter Chip Overview
2 spots with 2 different anchor sequences for DNA-encoded addressing.
Spot 1
Spot 2
Product Description
Order Number : AS-2-Rb-lfs
Table 1. Contents and Storage Information
| Material | Cap | Concentration | Amount | Buffer | Storage |
|---|---|---|---|---|---|
| Adapter strand 2 – Rb – lfs | |||||
| White | 200/250 nM | 5 x 200 µL | TE40 [1] | -20°C |
For research use only.
This product has a limited shelf life, please see expiry date on label.
To avoid many freeze thaw cycles please aliquot the nanolever.
Preparation | MIX&RUN
In-solution hybridization of adapter and ligand strands:
- Mix Adapter strand 1 – Rb (400 nM) and conjugated Ligand strand (500 nM) at 1:1 ratio (v/v).
- Incubate the solution of step 1 at RT at 600 rpm for 30 min to ensure complete hybridization.
- Mix solution of step 2 and Adapter strand 2 – Rb – lfs (200 nM) at 1:1 ratio (v/v).
Solution is ready to use for bio chip fictionalization.
Stability of the solution is related to the stability of the ligand molecule.
Table 2. Additional material for fictionalization of spot 1 and reference spot 2.
| Material | Concentration | Buffer | Related Product Name | Order No |
|---|---|---|---|---|
| Adapter strand 1 – Rb | 400 nM | TE40 [1] | ** | |
| Adapter strand 1** with red dye Rb | AS-1-Rb | |||
| Ligand strand carrying the conjugated ligand | 500 nM | 2P] E40 [ | ||
| heliX® Amine Coupling Kit 1 | HK-NHS-1 |
Example
Required volume for 3 functionalizations: 100 µL with a final concentration of 100 nM.
| Vial 1 | Vial 2 |
|---|---|
| Adapter strand 1 – Rb (400 nM) | Conjugated |
| Ligand strand (500 nM) | Adapter strand 1 |
– Rb – lfs (200/250 nM)
25 µL| 25 µL| 50 µL
After incubation time, mix vial 1 and vial 2 to obtain 100 µL of ready-to-use DNA solution.
Contact
Dynamic Biosensors GmbH
Perchtinger Str. 8/10
81379 Munich
Germany
Dynamic Biosensors, Inc.
300 Trade Center, Suite 1400
Woburn, MA 01801
USA
Order Information order@dynamic-biosensors.com
Technical Support support@dynamic-biosensors.com
www.dynamic-biosensors.com
Instruments and chips are engineered and manufactured in Germany.
©2024 Dynamic Biosensors GmbH | Dynamic Biosensors, Inc. All rights reserved.
- TE 40: 10 mM Tris, 40 mM NaCl, 0.05 % Tween20, 50 μM EDTA, 50 μM EGTA
- If the protein is not stable in PE40 (TE40, HE40), please check buffer compatibility with the switchSENSE® compatibility sheet.
AS-2-Rb-lfs v5.1 www.dynamic-biosensors.com
References
Read User Manual Online (PDF format)
Read User Manual Online (PDF format) >>