dynamic BIOSENSORS AS-1-Gb Adapter Strand 1 User Manual
- September 6, 2024
- dynamic BIOSENSORS
Table of Contents
dynamic BIOSENSORS AS-1-Gb Adapter Strand 1
Key Features
- Adapter strand 1 – Gb for functionalization of heliX® Adapter Chip Spot 1.
- Compatible with heliX® Adapter Chip.
- Includes Adapter strands for 50 regenerations.
- Ideal for MIX&RUN sample preparation.
- Adapter strand 1 carries a moderately hydrophilic green dye (Gb) with a negative net charge.
heliX ® Adapter Chip Overview
2 spots with 2 different anchor sequences for DNA-encoded addressing.
Product Description
Order Number: AS-1-Gb
Table 1. Contents and Storage Information
Material| Cap| Concentration| Amount| Buffer|
Storage
---|---|---|---|---|---
Adapter strand 1 – Gb| Black| 400 nM| 5 x 100 µL| TE40 [1]| -20°C
- For research use only.
- This product has a limited shelf life, please see expiry date on label.
- To avoid many freeze thaw cycles please aliquot the nanolayer.
Preparation | MIX&RUN
In-solution hybridization of adapter and ligand strands:
- Mix Adapter strand 1 – Gb (400 nM) and conjugated Ligand strand (500 nM) at 1:1 ratio (v/v).
- Incubate the solution of step 1 at RT at 600 rpm for 30 min to ensure complete hybridization.
- Mix solution of step 2 and Adapter strand 1 – Gb – lfs (200 nM) at 1:1 ratio (v/v).
Solution is ready to use for biochip functionalization.
Stability of the solution is related to the stability of the ligand molecule.
Table 2. Additional material for functionalization of spot 1 and reference spot 2.
Material| Concentration| Buffer| Related Product Name|
Order No
---|---|---|---|---
Ligand strand carrying the conjugated ligand| 500 nM| PE40 [2]| heliX® Amine
Coupling Kit 1| HK-NHS-1
Adapter strand 1 – Gb – lfs| 200/250 nM| TE40 [1]| Adapter strand 2 with green
dye Gb prehybridize with ligand-free strand| AS-2-Gb-lfs
Example
Required volume for 3 functionalizations: 100 µL with a final concentration of 100 nM.
| Vial 1 | Vial 2 |
|---|---|
| Adapter strand 1 – Gb (400 nM) | Conjugated Ligand strand (500 nM) |
strand 1 – Gb – lfs (200/250 nM)
25 µL| 25 µL| 50 µL
After incubation time, mix vial 1 and vial 2 to obtain 100 µL of ready-to-use DNA solution.
Contact
Dynamic Biosensors GmbH
Perchtinger Str. 8/10
81379 Munich
Germany
Dynamic Biosensors, Inc.
300 Trade Center, Suite 1400
Woburn, MA 01801
USA
Order Information order@dynamic-biosensors.com
Technical Support support@dynamic-biosensors.com
www.dynamic-biosensors.com
Instruments and chips are engineered and manufactured in Germany.
©2024 Dynamic Biosensors GmbH | Dynamic Biosensors, Inc. All rights reserved.
[1] TE40: 10 mM Tris, 40 mM NaCl, 0.05 % Tween20, 50 μM EDTA, 50 μM EGTA
[2] If the protein is not stable in PE40 (TE40, HE40), please check
buffer compatibility with the switchSENSE® compatibility sheet.
References
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