dynamic BIOSENSORS AS-2-Gb Adapter Strand 2 User Manual
- September 9, 2024
- dynamic BIOSENSORS
Table of Contents
dynamic BIOSENSORS AS-2-Gb Adapter Strand 2
Key Features
- Adapter strand 2 for functionalization of heliX ® Adapter Chip Spot 2.
- Compatible with heliX ® Adapter Chip.
- Includes Adapter strands for 50 regenerations.
- Ideal for MIX & RUN sample preparation.
- Adapter strand 2 carries a moderately hydrophilic green dye (Gb) with a negative net charge.
heliX ® Adapter Chip Overview
2 spots with 2 different anchor sequences for DNA-encoded addressing.
Product Description
Order Number: AS-2-Gb
Table 1. Contents and Storage Information
| Material | Cap | Concentration | Amount | Buffer | Storage |
|---|---|---|---|---|---|
| Adapter strand 2 – Gb | White | 400 nM | 5 x 100 µL | TE40 [1] | -20°C |
For research use only.
This product has a limited shelf life, please see expiry date on label.
To avoid many freeze thaw cycles please aliquot the nanolever.
Preparation | MIX & RUN
In-solution hybridization of adapter and ligand strands:
- Mix Adapter strand 1 – Gb (400 nM) and conjugated Ligand strand with ligand 1 (500 nM) at 1:1 ratio (v/v).
- Mix Adapter strand 2 – Gb (400 nM) and conjugated Ligand strand with ligand 2 (500 nM) at 1:1 ratio (v/v).
- Incubate separately the two solutions of step 1 and 2 at RT at 600 rpm for 30 min to ensure complete hybridization.
- Mix solution of step 1 and 2 at 1:1 ratio (v/v).
Solution is ready to use for biochip functionalization.
Stability of the solution is related to the stability of the ligand molecules.
Table 2. Additional material for functionalization of spot 1 and reference spot 2.
| Material | Concentration | Buffer | Related Product Name | Order No |
|---|---|---|---|---|
| Adapter strand 1 – Gb | 400 nM | TE40 [ 1] | ** _Adapter strand | |
| 2_ with green dye Gb** | AS-1-Rb |
Ligand strand
carrying the conjugated ligand 1| 500 nM| P 2] E40 [| heli X
® Amine Coupling Kit 1| HK-NHS-1
Ligand strand
carrying the conjugated ligand 2| 500 nM| PE40 [ 2]| heli X ®
Amine Coupling Kit 1| HK-NHS-1
Example
Required volume for 3 functionalizations: 100 µL with a final concentration of 100 nM.
| Vial 1 | Vial 2 |
|---|---|
| Adapter strand 1 – Gb (400 nM) | Conjugated Ligand strand with |
| ligand 1 (500 nM) | Adapter strand 2 – Gb (400 nM) |
Ligand strand + with ligand 2 (500 nM)
25 µL| 25 µL| 25 µL| 25 µL
Contact
- TE40: 10 mM Tris, 40 mM NaCl, 0.05 % Tween 20, 50 μM EDTA, 50 μM EGTA
- If the protein is not stable in PE40 (TE40, HE40), please check buffer compatibility with the switchSENSE® compatibility sheet.
Dynamic Biosensors GmbH
Perchtinger Str. 8/10 81379 Munich Germany
Dynamic Biosensors, Inc.
300 Trade Center, Suite 1400 Woburn, MA 01801 USA
Order Information order@dynamic-biosensors.com
Technical Support support@dynamic-biosensors.com
www.dynamic-biosensors.com
Instruments and chips are engineered and manufactured in Germany.
©2024 Dynamic Biosensors GmbH | Dynamic Biosensors, Inc. All rights reserved.
References
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