dynamic BIOSENSORS AS-2-Ra-lfs Adapter Strand 2 User Manual
- September 7, 2024
- dynamic BIOSENSORS
Table of Contents
dynamic BIOSENSORS AS-2-Ra-lfs Adapter Strand 2
Specifications:
- Product Name: heliX+ ADAPTER STRAND 2
- Order Number: AS-2-Ra-lfs
- Key Features:
- 2 spots with 2 different anchor sequences for DNA-encoded addressing
Usage Instructions:
Preparation | MIX &RUN
- Mix Adapter strand 1 – Ra (400 nM) and conjugated Ligand strand (500 nM) at a 1:1 ratio (v/v).
- Incubate the solution of step 1 at room temperature at 600 rpm for 30 min to ensure complete hybridization.
- Mix the solution of step 2 and Adapter strand 2 – Ra – lfs (200 nM) at a 1:1 ratio (v/v).
- The solution is now ready to use for biochip functionalization. The stability of the solution is related to the stability of the ligand molecule.
FAQ
- Q: What is the shelf life of Adapter Strand 2 – Ra – lfs?
- A : This product has a limited shelf life, please see the expiry date on the label.
- Q: How should I store the product to maintain its stability?
- A : To avoid many freeze-thaw cycles, please aliquot the nanolever.
- Q: What are the contact details for Dynamic Biosensors?
- A : You can contact Dynamic Biosensors GmbH in Germany at Perchtinger Str. 8/10, 81379 Munich or Dynamic Biosensors, Inc. in the USA at 300 Trade Center, Suite 1400, Woburn, MA 01801. For ordering information, email order@dynamic-biosensors.com. For technical support, email support@dynamic-biosensors.com.
Key Features
- Adapter strand 2 for functionalization of heliX® Adapter Chip Spot 2.
- Compatible with heliX® Adapter Chip.
- Includes Adapter strands for 50 regenerations.
- Ideal for MIX&RUN sample preparation.
- Adapter strand 2 carries a moderately hydrophilic red dye (Ra) with a single positive net charge.
heliX® Adapter Chip Overview
2 spots with 2 different anchor sequences for DNA-encoded addressing.
Product Description
Order Number: AS-2-Ra-lfs
Table 1. Contents and Storage Information
| Material | Cap | Concentration | Amount | Buffer | Storage |
|---|---|---|---|---|---|
| Adapter strand 2 – Ra – lfs | White | 200/250 nM | 5 x 200 µL | TE40 [ | |
| 1] | -20°C |
- For research use only.
- This product has a limited shelf life, please see the expiry date on the label.
- To avoid many freeze thaw cycles please aliquot the nanolever.
Preparation | MIX&RUN
In-solution hybridization of adapter and ligand strands:
- Mix Adapter strand 1 – Ra (400 nM) and conjugated Ligand strand (500 nM) at a 1:1 ratio (v/v).
- Incubate the solution of step 1 at RT at 600 rpm for 30 min to ensure complete hybridization.
- Mix the solution of step 2 and Adapter strand 2 – Ra – lfs (200 nM) at 1:1 ratio (v/v).
The solution is ready to use for biochip functionalization. The stability of the solution is related to the stability of the ligand molecule.
Table 2. Additional material for functionalization of spot 1 and reference spot 2.
| Material | Concentration | Buffer | Related Product Name | Order No |
|---|---|---|---|---|
| Adapter strand 1 – Ra | 400 nM | TE40 [ 1] | ** _Adapter strand | |
| 1_ with red dye Ra** | AS-1-Ra |
Ligand strand
carrying the conjugated ligand
| 500 nM| P 2] E40 [| heli X ® Amine Coupling Kit 1| HK-NHS-1
Example
Required volume for 3 functionalizations: 100 µL with a final concentration of
100 nM.
| Vial 1 | Vial 2 |
|---|
Adapter strand 1 – Ra
(400 nM)
| Conjugated Ligand strand
(500 nM)
| Adapter strand 2 – Ra – lfs
(200/250 nM)
25 µL| 25 µL| 50 µL
After incubation time, mix vial 1 and vial 2 to obtain 100 µL of ready-to-use DNA solution.
Contact
Dynamic Biosensors GmbH
- Perchtinger Str. 8/10 81379 Munich
- Germany
Dynamic Biosensors, Inc.
- 300 Trade Center, Suite 1400 Woburn, MA 01801
- USA
- Order Information order@dynamic-biosensors.com
- Technical Support support@dynamic-biosensors.com
- www.dynamic-biosensors.com
- Instruments and chips are engineered and manufactured in Germany.
- ©2024 Dynamic Biosensors GmbH | Dynamic Biosensors, Inc. All rights reserved.
TE40: 10 mM Tris, 40 mM NaCl, 0.05 % Tween20, 50 μM EDTA, 50 μM EGTA If the protein is not stable in PE40 (TE40, HE40), please check buffer compatibility with the switchSENSE® compatibility sheet.
References
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