dynamic BIOSENSORS AS-2-Gc-lfs Adapter Strand 2 User Manual
- September 7, 2024
- dynamic BIOSENSORS
Table of Contents
dynamic BIOSENSORS AS-2-Gc-lfs Adapter Strand 2
Specifications
- Product Name: heliX+ ADAPTER STRAND 2 with green dye Gc
- Manufacturer : Dynamic Biosensors GmbH & Inc.
- Order Number: AS-2-Gc-lfs
- Version : v5.1
Preparation and Usage Instructions
- Mix Adapter strand 1 – Gc (400 nM) and conjugated Ligand strand (500 nM) at a 1:1 ratio (v/v).
- Incubate the solution from step 1 at room temperature at 600 rpm for 30 minutes for complete hybridization.
- Mix the solution from step 2 with Adapter strand 2 – Gc – lfs (200 nM) at a 1:1 ratio (v/v).
- The solution is now ready for biochip functionalization.
- Note: The stability of the solution is dependent on the stability of the ligand molecule.
FAQ
- What is the shelf life of Adapter strand 2 – Gc- lfs?
- The product has a limited shelf life. Please refer to the expiry date on the label for specific information.
- Can Adapter strand 2 – Gc- lfs be used for purposes other than research?
- No, this product is intended for research use only.
Key Features
- Adapter strand 2 for functionalization of heliX® Adapter Chip Spot 2.
- Compatible with heliX® Adapter Chip.
- Includes Adapter strands for 50 regenerations.
- Ideal for MIX&RUN sample preparation.
- Adapter strand 2 carries a hydrophobic green dye (Gc) with a neutral net charge.
heliX® Adapter Chip Overview
2 spots with 2 different anchor sequences for DNA-encoded addressing.
Product Description
Order Number: AS-2-Gc-lfs
Table 1. Contents and Storage Information
| Material | Cap | Concentration | Amount | Buffer | Storage |
|---|---|---|---|---|---|
| Adapter strand 2 – Gc- lfs | White | 200/250 nM | 5 x 200 µL | TE40 [ | |
| 1] | -20°C |
or research use only. This product has a limited shelf life, please see the expiry date on the label. To avoid many freeze-thaw cycles please aliquot the nanolever.
Preparation | MIX&RUN
In-solution hybridization of adapter and ligand strands:
- Mix Adapter strand 1 – Gc (400 nM) and conjugated Ligand strand (500 nM) at 1:1 ratio (v/v).
- Incubate the solution of step 1 at RT at 600 rpm for 30 min to ensure complete hybridization.
- Mix the solution of step 2 and Adapter strand 2 – Gc – lfs (200 nM) at 1:1 ratio (v/v).
The solution is ready to use for biochip functionalization. Stability of the solution is related to the stability of the ligand molecule.
Table 2. Additional material for functionalization of spot 1 and reference spot 2.
| Material | Concentration | Buffer | Related Product Name | Order No |
|---|---|---|---|---|
| Adapter strand 1 – Gc | 400 nM | TE40 [ 1] | ** _Adapter strand | |
| 1_ with green dye Gc** | AS-1-Gc |
Ligand strand
carrying the conjugated ligand
| 500 nM| P 2] E40 [| heli X ® Amine Coupling Kit 1| HK-NHS-1
Example
Required volume for 3 functionalizations: 100 µL with a final concentration of
100 nM.
| Vial 1 | Vial 2 |
|---|
Adapter strand 1 – Gc
(400 nM)
| Conjugated Ligand strand
(500 nM)
| Adapter strand 2 – Gc – lfs
(200/250 nM)
25 µL| 25 µL| 50 µL
After incubation time, mix vial 1 and vial 2 to obtain 100 μL of ready-to-use DNA solution.
Contact
Dynamic Biosensors GmbH
- Perchtinger Str. 8/10 81379 Munich
- Germany
Dynamic Biosensors, Inc
- 300 Trade Center, Suite 1400 Woburn, MA 01801 USA
- Order Information order@dynamic-biosensors.com
- Technical Support support@dynamic-biosensors.com
- www.dynamic-biosensors.com
- Instruments and chips are engineered and manufactured in Germany.
©2024 Dynamic Biosensors GmbH | Dynamic Biosensors, Inc. All rights reserved.[1] TE40: 10 mM Tris, 40 mM NaCl, 0.05 % Tween20, 50 μM EDTA, 50 μM EGTA [2] If the protein is not stable in PE40 (TE40, HE40), please check buffer compatibility with the switchSENSE® compatibility sheet.
References
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