AFFIVET AFFIGEN Rapid Test Kit Instructions

AFFIVET AFFIGEN Rapid Test Kit

Product Information

Specifications

• Product Name: Porcine Japanese Encephalitis Virus Antibody
• Model: AFG-VGS-49
• Tests: 40 tests

Storage & Stability

• Store the components in a cool, dry place away from direct sunlight. Check the expiration date on the package before use.

Principle

• The test card detects antibodies against porcine Japanese Encephalitis Virus using a colorimetric reaction.

Sample Collection & Preparation

• Whole Blood: Collect anticoagulated whole blood. Sample without anticoagulants can also be used, but must be tested immediately.
• Serum: Collect whole blood, allow it to coagulate, then centrifuge at 2000-3000 r/min for 5-15 minutes or let it separate naturally at 4°C overnight.

Test Procedure

1. Open the package and place the test card on a clean, flat surface.
2. Use the dropper to absorb the sample.
3. Add 3 drops into the well marked with S.
4. Read the results within 10 to 20 minutes at room temperature.
5. The result is invalid after 20 minutes.

Result Interpretation

• Negative Result: C-Line present, T-Line absent
• Positive Result: C-Line present, T-Line present
• Weak Positive Result: C-Line present, T-Line present but light color
• Invalid Result: No C-Line or both C-Line and T-Line absent

Cautions

• Follow the instructions carefully to ensure accurate results.
• Contact us for any questions or concerns.

Contact Information

• Phone: 800-660-1620
• Email: info@affigen.com
• Website:www.affigen.com

FAQ

Q: What should I do if the test line doesn’t appear visible wine red?

A: If the test line is not visible wine red, it indicates there are no antibodies against the porcine JEV virus present. Consult with a healthcare provider for further guidance.

Material Required

• (40) JEV Ab Test Card
• (40) Dropper
• (40) Antibody titers Colorimetric card
• (01) Manual

Storage & Stability

1. Store the test kit at 4 to 30°C. DO NOT FREEZE.
2. Do not store the test kit in direct sunlight.
3. The test kit is stable within the expiration date marked on the package label.

Principle

• The AffiVET® Porcine Japanese Encephalitis Virus Antibody Rapid Test Card is an immuno-chromatographic test, using the colloidal gold immunoassay method, to detect the antibody against Japanese encephalitis virus in whole blood, and serum.
• After adding the sample, the sample moves along with the colloidal gold labeling JEV antigen, is there is a JEV antibody in the sample, it combines with the antigen on the line and shows a wine-red color; if there is no JEV antibody, then no color reaction.

Sample Collection & Preparation

Whole blood

• Collect anticoagulated whole blood.
• Taking whole blood without anticoagulants as a sample is also OK, but must test it immediately.

Serum

• Collect whole blood.
• After coagulation, centrifuge the tube at 2000-3000 revolutions per minute (r/min) for 5~15min or separate naturally at 4℃ overnight.

Test Procedure

1. Open the package, take out the test card, and put it on the clean, flat desk.
2. Use the dropper to absorb the sample.
3. Add 3 drops into the well marked with “S”.
4. Read the results in 10 to 20 min at room temperature.
5. The result is invalid after 20 min.

Result Interpretation

Negative Result

• Presence of C-Line
• Absence of T-Line

Positive Result

• Presence of C-Line
• Presence of T-Line

If not inoculated by the bacterin against porcine JEV virus:

1. Test lines don’t appear visible wine red shows there are no antibodies against porcine JEV virus in tested samples, if the swine are healthy, it is better to vaccinate in time.
2. Test lines appear visible in wine red showing the pig was infected with a wild virus, which should be more observed to validate.

I have been inoculated by the bacterin against the porcine JEV virus:

1. The color of the test line is thicker than the color of the titer 1:40 from the comparison card showing the high titer of the antibody against the porcine JEV virus, which is enough to resist the strong virus.
2. The color of the test line is thinner than the color of the titer 1:40 from the comparison card showing that the titer of the antibody against the porcine JEV virus is not enough to resist the strong virus, it is better to perform complementary vaccination.

Weak Positive Result

• Presence of C-Line
• Presence of T-Line

The color of the test line is very light.

Invalid Result

• Absence of C-Line
• Presence of T-Line
• Absence of C-Line
• Absence of T-Line
• This means that the procedure was not applied correctly or that the test is invalid.

Cautions

• Do not utilize test cards that are expired or in damaged packaging.
• Avoid using pure water, running water, or normal saline as negative controls.
• Store the test cards at room temperature (< 30 ℃ ). If stored in a cool environment, allow them to return to room temperature before opening to prevent moisture buildup.
• Use the test card promptly after opening the package.
• Refrain from touching the white membrane surface in the center of the test card.
• Ensure high-quality test results by using fresh, uncontaminated samples. High levels of blood lipids can impact results; therefore, after centrifugation, use serum for testing to avoid interference from fats.
• If the blood or serum is insufficient or sticky, dilute it with normal saline at the same dilution factor before testing.
• If serum cannot be tested immediately, store it at 4 ℃ for a short period (up to 48 hours) or below -20℃ for long-term storage.
• Test whole blood without anticoagulants immediately. If there is already clotting, separate serum for testing.
• Whole blood with anticoagulants should be tested within 24 hours and should not be frozen, as freezing and thawing can cause hemolysis, which affects results.
• Maintain a distance of 1cm between the dropper tip and the “S” well when adding samples to ensure accuracy. Too close proximity may result in inaccuracies due to small droplet volumes or prevent sample addition altogether, affecting test results.
• Avoid bubble formation when adding samples, and control the sample quantity to approximately 100ul (about 3 drops).
• Excessive sample volume does not enhance sensitivity and can lead to erythrocyte sedimentation, causing deep red backgrounds that interfere with results.
• This test card is intended only for porcine samples; its suitability for other animals has not been evaluated.
• The antibody level correlates only with the darkness of the T line, not the C line. A visible C line indicates a valid test.
• Dispose of test cards and samples according to local pollutant regulations.

Contact us

• Phone800-660-1620
• Websitewww.affigen.com
• Emailinfo@affigen.com.

References

• AffiGEN - Next-Generation Antibody Discovery and Development

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