AffiVET AFG-VR-43 CPV Antigen Rapid Test Kit Instructions

Table of Contents

AffiVET-AFG-VR-43-CPV-Antigen-Rapid-Test-Kit-Product

Storage:

Principle:
The CPV Antigen test kit is designed for detecting Canine Parvovirus antigen in dogs.

Precautions:

This test kit is exclusively for use in dogs.
The test device is sensitive to humidity and heat; initiate the test promptly after removing it from the foil pouch.
Components are single-use only and should not be reused.

Sample Collection & Preparation:

Test Procedure:

Result Interpretation:

Negative Result: Presence of C-Line, Absence of T-Line
Positive Result: Presence of C-Line, Presence of T-Line
Invalid Result: Absence of C-Line, Presence of T-Line or both Absence of C-Line and Absence of T-Line

Limitations:

Q: What should I do if the test kit is damaged or the seal is broken?

A: Do not use the test kit, as its sterility and integrity may be compromised. Contact customer support for assistance.

Q: Can I reuse any components of the test kit?

A: No, all components are single-use only and should not be reused under any circumstances.

Q: How should I handle samples to ensure safety?

A: Treat all samples as potentially infectious. Wear protective gloves during handling and wash hands thoroughly afterwards.

The AffiVET® Rapid CPV Ag Test Kit is a chromatographic immunoassay designed for the qualitative detection of Canine Parvovirus antigen in canine fecal samples.
This kit is equipped with two distinct lines on the device’s surface, namely the test (T) line and the control (C) line, which are initially invisible in the result window until a sample is applied. The control line serves as a crucial reference, confirming the test’s proper execution and is expected to appear with every test conducted. The presence of CPV antigens in the sample will result in the emergence of a purple test line within the result window.
To ensure high sensitivity and specificity, the assay employs highly selective antibodies against CPV, functioning both as capture and detector elements. These antibodies are adept at identifying CPV antigens in canine feces, thereby ensuring the assay’s high accuracy.

This test kit is exclusively designed for use in dogs. It is not suitable for testing other animals.
The test device is sensitive to both humidity and heat. To ensure accuracy, initiate the test promptly after removing the device from its protective foil pouch.
The components of this test kit are single-use only and should not be reused under any circumstances.
When applying the sample, use the provided disposable dropper and hold it vertically to ensure precision.
Avoid making contact with the membrane located in the result window of the test device to prevent contamination or damage.
Do not use the test kit after its expiration date, which is indicated on the package label, to guarantee the reliability of results.
If the pouch is damaged or the seal has been tampered with, do not use the test kit as its sterility and integrity may be compromised.
Refrain from interchanging components between kits of different lot numbers. Each kit’s components have undergone quality control testing as a complete unit to ensure their performance.
Treat all samples as potentially infectious. Protective gloves should be worn during sample handling, and hands should be washed thoroughly afterwards.
Follow national and local guidelines for the safe decontamination and disposal of all samples, used kits, and any materials that might be contaminated to ensure environmental safety and health protection.

Utilize a canine feces swab for this test.
Test samples immediately after collection.
If immediate testing isn’t possible, store samples at 2 to 8°C for 24 hours. For longer storage, freeze at -20°C or below. Before use, thaw frozen samples to room temperature (15 to 30°C).
Ensure the correct amount of fecal material on the swab as depicted in the accompanying image. Excessive fecal matter may lead to false positive results and hinder migration. $AffiVET-AFG-VR-43-CPV-Antigen-Rapid-Test-Kit-Fig- $1$$

Ensure all reagents and samples are brought to room temperature (15~30°C) before use.
Collect fecal samples using a swab.
Insert the swab into the assay diluent tube and thoroughly mix until the sample is dissolved into the diluent (approximately 10 seconds).
Allow the mixture to settle for 1 minute to allow large particles to precipitate.
Remove the test device from the foil pouch and place it on a flat, dry surface.
Using the disposable dropper, transfer the supernatant sample from the tube.
Add 4 drops of the mixed sample into the sample hole(s), dropping vertically.
Begin the timer and allow the sample to flow across the result window. If no flow is observed after 1 minute, add one additional drop of the mixed sample to the sample hole.
Interpret test results at 10 minutes. Do not read the results after 20 minutes.

$AffiVET-AFG-VR-43-CPV-Antigen-Rapid-Test-Kit-Fig- $2$$

Negative Result
- Presence of C-Line
- Absence of T-Line
Positive Result
- Presence of C-Line
- Presence of T-Line
Invalid Result
- Absence of C-Line
- Presence of T-Line
- Absence of C-Line
- Absence of T-Line

While the AffiVET Rapid CPV Ag Test kit is highly accurate in detecting Canine Parvovirus antigen, occasional false results may occur. In cases of uncertainty, additional clinical or laboratory tests may be necessary. It is important to note that a definitive clinical diagnosis should not rely solely on the results of a single test but should be made by the veterinarian after considering all clinical and laboratory findings.
The reading window may exhibit a light pink background colouration, which will not impact the accuracy of the results.
AffiGEN Inc. and its distributors cannot be held liable for any consequences resulting from the misuse or misinterpretation of the test results.