ThermoFisher SCIENTIFIC Q33230 Qubit Assay Protocol User Guide
- June 6, 2024
- ThermoFisher SCIENTIFIC
Table of Contents
SCIENTIFIC Q33230 Qubit Assay Protocol
User Guide
General Qubit Assay Protocol
- Set up two assay tubes for the standards (three for the protein or RNA IQ assay) and one assay tube for each sample.
- Prepare the Qubit ™ working solution by diluting the Qubit ™ reagent 1:200 in the Qubit ™ buffer. Prepare 200 μL of working solution for each standard and sample. †
- Prepare the assay tubes* according to the table below.
- Vortex all tubes for 2–3 seconds.
- Incubate the tubes for 2 minutes at room temperature (15 minutes for the Qubit ™ protein assay).
- Insert the tubes in the Qubit ™ Fluorometer and take readings. For detailed instructions, refer to the Qubit ™ Fluorometer manual.
| Standard assay tubes| User sample assay tubes
---|---|---
Working solution † (from step 2)| 190 μL| 180–199 μL
Standard (from the kit)| 10 μL| —
User sample| —| 1–20 μL
Total Volume in each assay tube| 200 μL| 200 μL
†Qubit 1X dsDNA assays (Cat. Nos. Q33230, Q33231, Q33265, Q33266) are supplied with a ready-to-use working solution and do not require preparation.
- Use only thin-wall, clear 0.5 mL PCR tubes. Acceptable tubes include Qubit™ assay tubes (set of 500, Cat. No. Q32856).
For Qubit Protein BR Assay only
- Set up two assay tubes for the standards, one assay tube for a buffer blank and one assay tube for each sample.
- Prepare assay tubes according to the table below. Adding solutions in the following order to the assay tubes: (1) Standards/Sample/Sample Buffer, (2) Protein BR Assay Buffer, (3) Protein BR Assay Reagent.
- Vortex for 5-7 seconds immediately after addition of Protein BR Assay Reagent.
- Incubate tubes for 10 minutes at room temperature.
- Insert the tubes in the Qubit™ Fluorometer and takereadings. For detailed instructions, refer to the Qubit™ Fluorometer manual.
| Standard assay tubes| Buffer blank| User sample assay tubes
---|---|---|---
Standard (from the kit)| 20 µL| –| –
User sample buffer| –| 10 or 20 µL| –
User sample| –| –| 10 or 20 µL
Protein BR Assay Buffer| 150 µL| 150 or 160 µL| 150 or 160 µL
Protein BR Assay Reagent| 30 µL| 30 µL| 30 µL
Total volume in each assay tube| 200 µL| 200 µL| 200 µL
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The information in this document is subject to change without notice.
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Manufacturer: Multiple Life Technologies Corporation manufacturing sites
are responsible for manufacturing the products associated with the workflow
covered in this guide.
Find out more at thermofisher.com/qubit
For Research Use Only. Not for use in diagnostic procedures. © 2021 Thermo
Fisher Scientific Inc. All rights reserved. All trademarks are the property of
Thermo Fisher Scientific and its subsidiaries unless otherwise specified. 12
March 2021
References
- Qubit Fluorometric Quantification | Thermo Fisher Scientific - US
- Terms and Conditions of Sale | Thermo Fisher Scientific - US
- Help and Support | Thermo Fisher Scientific - UK
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