hygiena BAX-Q7 Molecular Pathogen Detection System Installation Guide

: June 16, 2024
: Hygiena

Table of Contents

hygiena BAX-Q7 Molecular Pathogen Detection System
Product Information
Product Usage Instructions
using instruction
Pooled Sample Protocol
Non-Pooled Sample Protocol
References
Read User Manual Online (PDF format)
Download This Manual (PDF format)

hygiena BAX-Q7 Molecular Pathogen Detection System

Product Information

Specifications

Product Name: Yeast and Mold PCR Assay
Action Levels: 10-50 cfu/g for pooled sample protocol, 25 cfu/g or above for non-pooled sample protocol
Enrichment Replicates: 3 disrupter tubes for pooled sample protocol
Sample Homogenate Dilution: 1:10 according to food type
Incubation Time: 44 hours
PCR Tablets Hydration Volume: 50 µl lysate from step 12

Product Usage Instructions

Step 1: Sample Homogenization
Homogenize the sample in a 1:10 dilution according to the food type.
Step 2: Determine Sample Volume
Determine the sample volume to be tested. Refer to the User Guide or table on the back of the reference card for guidance.
Step 3: Transfer Sample
Transfer the sample to a disrupter tube.
Step 4: Incubate Disrupter Tubes
Incubate the disrupter tubes for 44 hours.
Step 5: Add DNA Stabilizer
Add DNA stabilizer to the disrupter tubes.
Step 6: Agitate in Disrupter Device
Agitate the disrupter tubes in a disrupter device.
Step 7: Create a Rack File
Create a rack file for further processing.
Step 8: Transfer the Lysis Reagent
Transfer 12 mL of Protease YM lysis buffer to cluster tubes.
Step 9: Transfer Disrupted Samples
Transfer the disrupted samples from the disrupter tubes to the cluster tubes.
Step 10: Heat Cluster Tubes
Heat the cluster tubes for 20 seconds.
Step 11: Cool Cluster Tubes
Cool the cluster tubes in a cooling block.
Step 12: PCR Tablet Hydration
Hydrate PCR tablets with 50 µl lysate from step 12.
Step 13: Initialize Cycler
Initialize the cycler for PCR.
Step 14: Arrange PCR Tubes
A rrange PCR tubes in the cooling block.
Step 15: Place Tubes in the Cycler
Place the PCR tubes in the cycler and run the program.
Step 16: Review Results

Unload the samples and review the results on the screen. Refer to the User Guide for detailed information on result interpretation.

FAQ:

Q: What are the action levels for the pooled sample protocol?
A: The action levels for the pooled sample protocol are 10-50 cfu/g.
Q: Can the non-pooled sample protocol be used for action levels below 25 cfu/g?
A: No, the non-pooled sample protocol is recommended for action levels of 25 cfu/g or above.

using instruction

Homogenize the sample in 1:10 dilution according to the food type.
Determine the sample volume to be tested.
(See User Guide or table on the back of this reference card.)
Transfer the sample to the disrupter tube.
Pooled sample protocol requires triplicate disrupter tubes.
Incubate disrupter tubes.
Add DNA stabilizer to disrupter tubes.
DNA stabilizer
Agitate in disrupter device.
Create a rack file.
Add protease to YM lysis buffer.
Transfer lysis reagent made in step 8 to cluster tubes
Lysis reagent mixture can be stored at 2 – 8 °C for up to one week
Transfer disrupted samples to cluster tubes.
*Pooled sample protocol requires pooled volumes from disrupter tubes into 1 cluster tube.
Heat cluster tubes.
Cool cluster tubes in the cooling block.

Steps 11 and 12 can also be performed using the Hygiena™ Automated Thermal Block. See the Automated Thermal Block User Guide for details and instructions. 13. Initialize cycler. 14. Arrange PCR tubes in the cooling block. 15. Hydrate PCR tablets with 50 μl lysate from step 12. 16. Place tubes in the cycler and run the program. 17. Unload samples and review results on the screen. See User Guide for details.

Pooled Sample Protocol

This ultra-sensitive protocol uses pooled samples from three disrupter tube enrichment replicates for action levels of 10-50 cfu/g.

If your action level is:

| Then transfer this volume of homogenate to 3 disrupter tubes:| And pool these volumes of disrupted samples for testing:
---|---|---
10 cfu/g| 400 µL| 7 µL from 3 replicates
20 cfu/g| 200 µL| 7 µL from 3 replicates
50 cfu/g| 80 µL| 7 µL from 3 replicates