COBIOER CBP74201 SRE Reporter Cell Line Instruction Manual

: June 15, 2024
: COBIOER

Table of Contents

COBIOER CBP74201 SRE Reporter Cell Line
Specifications
Product Usage Instructions
Background
Introduction
Description of Host Cell Line
Representative Data
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COBIOER CBP74201 SRE Reporter Cell Line

Specifications

Product Name: SRE.L-Luc/HEK293 CBP74201
Host Cell: HEK293
Expressed gene: SRE.L-Luciferase
Stability: 32 passages (in-house test, not indicative of
stability beyond tested passages)
Synonym(s): SRE cell line, MAPK reporter, ERK reporter
Freeze Medium: 90% FBS+10% DMSO
Culture Medium: DMEM+10%FBS+100ug/ml hygromycin
Mycoplasma Testing: Negative
Storage: Liquid nitrogen
Application(s): Functional (Report Gene) Assay

Product Usage Instructions

Background
The SRE.L-Luc/HEK293 CBP74201 is a cell line that expresses the SRE.L-Luciferase gene. It has been tested for stability up to 32 passages, but its stability beyond that is not guaranteed. It is commonly referred to as the SRE cell line, MAPK reporter, or ERK reporter.

Introduction
The host cell for this product is HEK293, derived from Homo sapiens’ embryonic kidney tissue. The cells have normal morphology and are epitheloid in nature. They grow in an adherent manner.

Description of Host Cell Line

Organism: Homo sapiens (human)
Tissue: Embryonic kidney
Disease: Normal
Morphology: Epitheloid cell
Growth Properties: Adherent

Representative Data
Figure 1 shows the detection of Luciferase assay using the Ultra Luciferase Detection Kit CBPH0001 (recommended to purchase from Cobioer).

FAQ

Can I use a different culture medium for this cell line?
We recommend using DMEM+10%FBS+100ug/ml hygromycin as the culture medium for optimal growth and expression of the SRE.L-Luciferase gene.

How should I store the cell line?
The cell line should be stored in liquid nitrogen for long-term preservation. Make sure to follow proper cryopreservation protocols.

Is the cell line tested for mycoplasma contamination?
Yes, the cell line has been tested and confirmed to be negative for mycoplasma contamination.

SRE.L-Luc/HEK293
CBP74201

Background

The SRE Reporter – HEK293 cell line is designed for monitoring the activity of the MAPK/ERK signaling pathway. The SRE Reporter –HEK293 cell line contains a firefly luciferase gene under the control of SRE responsive elements stably integrated into HEK293 cells, resulting in an ERK pathway-responsive reporter cell line.

Introduction

Host Cell: HEK293
Expressed gene: SRE.L-Luciferase
Stability: 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.)
Synonym(s): SRE cell line, MAPK reporter, ERK reporter
Freeze Medium: 90% FBS+10% DMSO
Culture Medium: DMEM+10%FBS+100ug/ml hygromycin
Mycoplasma Testing: Negative
Storage: Liquid nitrogen
Application(s): Functional(Report Gene) Assay