COBIOER BIOSCIENCES IL22 Effector Reporter Cell Instructions

: June 9, 2024
: COBIOER BIOSCIENCES

Table of Contents

COBIOER BIOSCIENCES IL22 Effector Reporter Cell
IL22 Effector Reporter Cell CBP74175
Background
Introduction
Representative Data
Product Usage Instructions
Read User Manual Online (PDF format)
Download This Manual (PDF format)

COBIOER BIOSCIENCES IL22 Effector Reporter Cell

IL22 Effector Reporter Cell CBP74175

Background

Interleukin-22 (IL-22) is a protein that in humans is encoded by the IL22 gene. IL-22 primarily targets non-hematopoietic epithelial and stromal cells where it can promote proliferation and play a role in tissue regeneration. In addition, IL-22 regulates host defense at barrier surfaces. However, IL-22 has also been linked to several conditions involving inflammatory tissue pathology.

Introduction

Expressed gene: IL22R
Stability: 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.)
Synonym(s): Interleukin-22, IL-TIF, ILTIF, ZCYTO18
Freeze Medium: 90% FBS+10% DMSO
Culture Medium: DMEM+10%FBS+2ug/ml puromycin+200ug/ml hrgromycin+5ug/ml blasticidin
Mycoplasma Testing: Negative
Storage: Liquid nitrogen
Application(s): Functional(Report Gene) Assay

Representative Data

Figure 1. Recombinant IL22 Effector Reporter Cell constitutively expressing IL22R.
Figure 2. Recombinant IL22 Effector Reporter Cell constitutively expressing IL10RB.
Figure 3. Dose Response of Recombinant Human IL-22 in Human IL-22 Effector Reporter Cells(C8).
Figure 4. Inhibition of IL-22-induced Reporter Activity By IL-22 Neutralization Ab in IL-22 Effector Reporter Cells(C8).

Product Usage Instructions

IL22 Effector Reporter Cell CBP74175 is used for functional (report gene) assay. The cells should be stored in liquid nitrogen until ready to use. To culture the cells, use DMEM with 10% FBS and add the following antibiotics: 2ug/ml puromycin, 200ug/ml hrgromycin, and 5ug/ml blasticidin. The cells should be tested for mycoplasma contamination before use. The stability of the cell line has been tested up to 32 passages. When using the cells for experiments, refer to the representative data provided in the user manual for guidance.

IL22 Effector Reporter Cell CBP74175

Background
Interleukin-22 (IL-22) is protein that in humans is encoded by the IL22 gene.IL-22 primarily targets non-hematopoietic epithelial and stromal cells where it can promote proliferation and play a role in tissue regeneration. In addition, IL-22 regulates host defense at barrier surfaces. However, IL-22 has also been linked to several conditions involving inflammatory tissue pathology.

Introduction

Expressed gene: IL22R
Stability: 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.)
Synonym(s): Interleukin-22、IL-TIF、ILTIF、ZCYTO18
Freeze Medium: 90% FBS+10% DMSO
Culture Medium :DMEM+10%FBS+2ug/ml puromycin+200ug/ml hrgromycin+5ug/ml blasticidin
Mycoplasma Testing: Negative
Storage : Liquid nitrogen

Representative Data Figure 1. Recombinant IL22 Effector Reporter Cell constitutively expressing IL22R. COBIOER-BIOSCIENCES-IL22-Effector-Reporter-
Cell-fig-3 Figure 2. Recombinant IL22 Effector Reporter Cell constitutively expressing IL10RB. Figure 3. Dose Response of Recombinant Human IL-22 in Human IL-22 Effector Reporter Cells(C8 ).Figure 4. Inhibition of IL-22-induced Reporter Activity By IL-22 Neutralization Ab in IL-22 Effector Reporter Cells(C8).