COBIOER BIOSCIENCES CBP74097 THP1 Reporter Cells User Manual

June 9, 2024
COBIOER BIOSCIENCES

COBIOER BIOSCIENCES CBP74097 THP1 Reporter Cells

Background

THP1 reporter cells are derived from THP-1, a human monocytic cell line that naturally expresses many pattern-recognition receptors, including Toll-like receptors.

Description
The NF-κB reporter (Luc)-THP-1 cell line is designed for monitoring nuclear factor Kappa B (NF-κB) signal transduction pathways. It contains a firefly luciferase gene driven by four copies of the NF-κB response element located upstream of the minimal TATA promoter. After activation by pro-inflammatory cytokines or stimulants of lymphokine receptors, endogenous NF-κB transcription factors bind to the DNA response elements, inducing transcription of the luciferase reporter gene.

Introduction

  • Host Cell: THP-1
  • Expressed gene: ISRE
  • Stability: 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.)
  • Synonym(s): NF-kB reporter, NFkappaB reporter, luciferase reporter, NF-kB reporter, NFkB reporter, nfkb thp1, thp1, NF-kB, nfkb, nf-kb Freeze Medium: 90% FBS+10% DMSO
  • Culture Medium: RPMI-1640+10%FBS+10 µg/ml of blasticidin+ 100 μ g/ml of Zeocin
  • Mycoplasma Testing: Negative
  • Storage: Liquid nitrogen
  • Application(s): Functional(Report Gene) Assay

Description of Host Cell Line

  • Organism: Homo sapiens, human
  • Tissue: Peripheral blood
  • Disease: Acute monocytic leukemia
  • Morphology: Monocyte
  • Growth Properties: Suspension

Representative DataCOBIOER-BIOSCIENCES-CBP74097-THP1-Reporter-Cells-
fig-1

Figure 1. Dose Response of 2’3-cGAMP in THP-1 ISRE-Luc/NFkB-SEAP Cells , the EC50 was 13.5μM.

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