azure CS1000 Personal Chemiluminescent Imager User Manual

azure CS1000 Personal Chemiluminescent Imager

Product Information

Product Name: chemiSOLO
Part Number: CS1000

Safety and Regulatory Compliance

• Important Safety Information: Please read these instructions before operating the chemiSOLO.
• Electrical Safety Precautions: Be sure to take proper precautions when handling any electrical equipment. NEVER work on any live circuit, fixture, receptacle, or switch.
• Protective Ground Terminal: The ground terminal, intended for connection to an external protective conductor for protection against electric shock in case of a fault, is located on the inside of the back panel.
• For Research Use Only: This instrument is suitable for research use only. It must be used, therefore, only by personnel who know the health risks associated with the reagents that are normally used with this instrument.
• Warranty: The chemiSOLO is warranted against defects in materials and workmanship for one year unless otherwise outlined on your sales order or www.azurebiosystems.com/warranty.
• Voltage Setting Information: The chemiSOLO has a power supply that automatically chooses the correct voltage for your country or region.
• CE Conformity: The chemiSOLO is in conformity with the provisions of the following EC Directives and the following harmonized standards have been applied.

Product Usage Instructions

Installation and Setup

• System Placement: Place the chemiSOLO instrument in a suitable location.
• Connecting to Power: Connect the power cord to a secure power outlet. Ensure that the system is connected to a well-grounded power source using a surge-protecting power strip.
• Turning the System On/Off: Locate the main power switch on the back panel, just above the power entry module. Use this switch to turn the system on or off.
• Software Access: Follow the instructions provided to access the software of the chemiSOLO instrument.
• External Wi-Fi Setup (optional): If desired, set up a Wi-Fi connection for the chemiSOLO instrument using the external Wi-Fi setup instructions.

Image Capture Overview

• Home Screen Overview: Familiarize yourself with the home screen interface of the chemiSOLO instrument.
• Chemiluminescent Imaging: Use the chemiSOLO instrument to capture chemiluminescent images.
• Visible Imaging: Use the chemiSOLO instrument to capture visible images.
• Custom Imaging: Utilize the custom imaging feature of the chemiSOLO instrument to capture images as per your requirements.
• Auto-Save: Learn how to enable the auto-save feature on the chemiSOLO instrument.

Image Gallery Overview

• Gallery: Access and manage the image gallery on the chemiSOLO instrument.
• Image Adjustment: Adjust and modify captured images using the image adjustment settings on the chemiSOLO instrument.

Settings Overview

• General: Explore and configure general settings on the chemiSOLO instrument.
• Software: Access and modify software settings on the chemiSOLO instrument.
• Create Darkmasters, Create Flats: Learn how to create darkmasters and flats using the chemiSOLO instrument.

Safety and Regulatory Compliance

Important Safety Information
Please read these instructions before operating the chemiSOLO.

Electrical Safety Precautions
Be sure to take proper precautions when handling any electrical equipment. NEVER work on any live circuit, fixture, receptacle, or switch. Safety rules you should follow whenever working with any electrical appliance include:

• Always shut off power at the main disconnect before changing a fuse.
• Always shut off power to the circuit before repairing or replacing a switch, receptacle, or fixture.
• Always tape over the main switch, empty fuse socket, or circuit breaker you are working on.
• Always check that the circuit is dead before beginning work on it. Using a circuit tester or voltmeter can help you determine this.
• Always unplug any appliance before repairing it.

Protective ground terminal

The ground terminal, intended for connection to an external protective conductor for protection against electric shock in case of a fault, is located on the inside of the back panel.

For Research Use Only
This instrument is suitable for research use only. It must be used, therefore, only by personnel who know the health risks associated with the reagents that are normally used with this instrument.

Warranty
The chemiSOLO is warranted against defects in materials and workmanship for one year unless otherwise outlined on your sales order or www.azurebiosystems.com/warranty. If any defect occurs in the instrument during this warranty period, Azure Biosystems, Inc. will repair or replace the defective parts at its discretion without charge. The following defects, however, are specifically excluded:

• Defects caused by improper operation.
• Repair or modification done by anyone other than Azure Biosystems or the company’s authorized agent.
• Use of spare parts supplied by anyone other than Azure Biosystems.
• Damage caused by accident or misuse.
• Damage caused by disaster.
• Corrosion caused by improper solvents or samples.
• Unauthorized movement of the chemiSOLO.

Voltage Setting Information
The chemiSOLO has a power supply that automatically chooses the correct voltage for your country or region.

CE Conformity
The chemiSOLO is in conformity with the provisions of the following EC Directives and the following harmonized standards have been applied:

• EN61010-1: 2012
• EN IEC 62311:2020
• EN 301489-1 V 2.2.3:2019
• EN301489-17 V 3.2.4:2020
• EN IEC 61000-3-2:2019+A1
• EN 61000-3-3:2013+A1
• EN IEC 61326-1:2021
• EN 300328 V 2.2.2:2019

FCC Part 15, Subpart B:2020

Contact
Azure Biosystems, Inc.
6747 Sierra Court, Suite A-B • Dublin, CA 94568 • USA
info@azurebiosystems.com • 925-307-7127 • Fax 925-905-1816

Introduction

The chemiSOLO offers high performance chemiluminescent and visible imaging in a compact design with unique external control methods. Each chemiSOLO instrument includes the following key components:

• Body – The body is a light-tight imaging station, containing the camera and lens, mirrors, electrical components, Ethernet, Wi-Fi emitter, and USB ports for data collection and external computer control.
• Stage – The imaging stage is a user-accessible, transparent platform for sample placement just beneath the inner lid, which contains the LED light sources.
• Camera – High resolution 6.29MPx camera, preinstalled in the system.
• LEDs – The LED module allows for visible imaging, utilized for chemi marker images and visible stained gel images in both color or grayscale.•
• USB Ports – There are two USB ports for data transfer or for the external Wi-Fi dongle [CS1006], both located at the back of the instrument.
• Ethernet Port – There is one ethernet port on the back of the instrument, which enables direct computer connection with the use of an ethernet cord [CS1007].
• USB Memory Stick – The Azure Biosystems memory stick contains calibration files relevant to your chemiSOLO system.

Table of Specifications

Contacting Technical Support
For questions regarding installation, setup, or general use of the chemiSOLO, please contact support@azurebiosystems.com or call 925-307-7127, 9am–4pm PST (GMT-8).

Installation and Setup

System Placement

• As with all electrical instruments, the chemiSOLO should be located away from water, solvents, or corrosive materials on a flat and stable surface with adequate clearance on all sides. The system must remain stationary during operation.
• The system should be placed away from interfering electrical signals and magnetic fields. If possible, a dedicated electrical outlet should be used to eliminate electrical interference from other instrumentation in your laboratory.
• The chemiSOLO should be installed at no more than 3,000 meters above sea level. The unit should be placed out of direct, bright sunlight or illumination sources to guard against light leaks.

Connecting to Power

• The power entry module is located in the lower right on the back panel of the system. Connect the power cord to a secure power outlet.
• It is important to connect the system to a well-grounded power source. Azure Biosystems recommends that you employ a surge-protecting power strip to protect against unexpected power surges that may cause damage to the electrical components.

Turning the System On/Off
The main power switch is located on the back panel, just above the power entry module

1. First, flip the power switch to On (I).
2. The light on the front of the chemiSOLO will turn blue and white while loading, then green once the instrument is ready for use.

To turn off the system, shut down the chemiSOLO by:

1. Disconnecting from the chemiSOLO software on the controlling device.
2. Then, shut off power to the system by flipping the power switch on the back of the instrument to Off (O).

Software Access
The chemiSOLO Capture Software uses a unique, web-based browser that connects through either a direct ethernet connection or through a Wi-Fi signal to an external device.

Ethernet Connection
To establish a connection to a chemiSOLO unit through ethernet, connect an ethernet cord to the ethernet plug located in the middle-bottom of the back of the instrument while the unit is powered off. Then, connect the other side of the ethernet cord to an external computer. Power on the chemiSOLO and wait until the light on the front turns green. From there there, open a web browser on the computer (Google Chrome recommended) and input the chemiSOLO’s ethernet IP address “192.168.40.1” to access the chemiSOLO control software.

Wi-Fi Connection
Alternatively, each chemiSOLO can be controlled by connecting to its unique Wi-Fi signal and accessing the chemiSOLO control software through a web browser. As such, it can be controlled by computers, tablets, or mobile devices. For directions on connecting your particular device via Wi-Fi, please find the appropriate section below.

Connecting a PC to the chemiSOLO

• PC System Requirements:

• 2GB RAM or up

• Windows 10 or up

• Ethernet-compatible or Wi-Fi-enabled

• Must have a web browser installed (Google Chrome recommended)

  1. Select the Network icon on the taskbar. If you do not see the Network icon or Disconnected icon, select the Up arrow to find the icon.

  2. (Windows 11) Access the Manage Wi-Fi connections menu using the right arrow (>).

  3. Select the Wi-Fi network specific to the desired chemiSOLO unit and select Connect. If a custom password was set previously, input the password and select Connect.
     Note : The default settings for the system’s unique Wi-Fi system will be “chemiSOLO_[system serial number]” with no password. For reference, each unit’s serial number can be located on the back of the instrument.

  4. Once the PC is properly connected to the chemiSOLO’s Wi-Fi, open a web browser and input the chemiSOLO’s Wi-Fi IP address “192.168.12.1” to access the control software.

Connecting a Mac to the chemiSOLO

• Mac System Requirements:

• 2GB RAM or up

• macOS 10.12 “Sierra” or up

• Ethernet-compatible or Wi-Fi-enabled

• Must have a web browser installed (Safari recommended)

  1. Select the Wi-Fi icon in the menu bar.

  2. Select the Wi-Fi network specific to the desired chemiSOLO unit and click Join. If a custom password was set previously, input the password and select Join.
     Note: The default settings for the system’s unique Wi-Fi system will be “chemiSOLO_[system serial number]” with no password. For reference, each unit’s serial number can be located on the back of the instrument.

  3. Once the Mac is properly connected to the chemiSOLO’s Wi-Fi, open a web browser and input thechemiSOLO’s Wi-Fi IP address “192.168.12.1” to access the control software.

Connecting an Android Phone to the chemiSOLO

• Android Phone System Requirements:

• Android 8.0 “Oreo” or up

• Ethernet-compatible or Wi-Fi-enabled

• Must have a web browser installed (Google Chrome recommended)

  1. Open the Settings app.

  2. Navigate to Wi-Fi menu.

  3. Select the Wi-Fi network specific to the desired chemiSOLO unit and select Connect. If a custom password was set previously, input the password and select Connect.
     Note: The default settings for the system’s unique Wi-Fi system will be “chemiSOLO_[system serial number]” with no password. For reference, each unit’s serial number can be located on the back of the instrument.

  4. Once the Android phone is connected to the chemiSOLO’s Wi-Fi, open a web browser and input the chemiSOLO’s Wi-Fi IP address “192.168.12.1” to access the control software or alternatively, scan the QR code on the inner lid of the unit to quickly access the same address.

Connecting an iPhone to the chemiSOLO

• iPhone System Requirements:

  •  iPhone 8 or up
  • iOS 12 or up
  • Ethernet-compatible or Wi-Fi-enabled
  • Must have a web browser installed (Safari recommended)

  1.  From the Home screen, open the Settings app.

  2. Navigate to the Wi-Fi menu.

  3.  Select the Wi-Fi network specific to the desired chemiSOLO unit. If a custom password was set previously, input the password and select Join.
     Note: The default settings for the system’s unique Wi-Fi system will be “chemiSOLO_[system serial number]” with no password. For reference, each unit’s serial number can be located on the back of the instrument.

  4. Once the iPhone is connected to the chemiSOLO’s Wi-Fi, open a web browser and input the chemiSOLO’s Wi-Fi IP address “192.168.12.1” to access the control software or alternatively, scan the QR code on the inner lid of the unit to quickly access the same address.

Connecting an Android Tablet to the chemiSOLO

Android Tablet System Requirements:

• Android 8.0 “Oreo” or up

• Ethernet-compatible or Wi-Fi-enabled

• Must have a web browser installed (Google Chrome recommended)

  1. Open the Settings app.

  2. Navigate to the Wi-Fi menu.

  3. Select the Wi-Fi network specific to the desired chemiSOLO unit and select Connect. If a custom password was set previously, input the password and select Connect.
     Note: The default settings for the system’s unique Wi-Fi system will be “chemiSOLO_[system serial number]” with no password. For reference, each unit’s serial number can be located on the back of the instrument.

  4. Once the Android tablet is connected to the chemiSOLO’s Wi-Fi, open a web browser and input the chemiSOLO’s Wi-Fi IP address “192.168.12.1” to access the control software or alternatively, scan the QR code on the inner lid of the unit to quickly access the same address.

Connecting an iPad to the chemiSOLO
iPad System Requirements:

• iPad (5th generation) or up

• iPadOS 13 or up

• Ethernet-compatible or Wi-Fi-enabled

• Must have a web browser installed (Safari recommended)

  1. From the Home screen, open the Settings app.

  2. Navigate to the Wi-Fi menu.

  3. Select the Wi-Fi network specific to the desired chemiSOLO unit. If a custom password was set previously, input the password and select Join.
     Note: The default settings for the system’s unique Wi-Fi system will be “chemiSOLO_[system serial number]” with no password. For reference, each unit’s serial number can be located on the back of the instrument.

  4. Once the iPad is connected to the chemiSOLO’s Wi-Fi, open a web browser and input the chemiSOLO’s Wi-Fi IP “192.168.12.1” to access the control software or alternatively, scan the QR code on the inner lid of the unit to quickly access the same address.

External Wi-Fi Setup (optional)
Wi-Fi Dongle [CS1006] Setup
With the chemiSOLO Wi-Fi dongle accessory [CS1006] attached, the chemiSOLO can connect to an external Wi-Fi network. This will allow devices connected to the chemiSOLO to simultaneously access both the chemiSOLO control software and the internet via the connected network. Crucially, please keep in mind that this setup will only work for the chemiSOLO Wi-Fi dongle accessory [CS1006], specifically, and not for other Wi-Fi dongles. To set up the external network, follow the steps below:

1. First, power on and connect to the chemiSOLO on an external device device (see section 2.4; computer recommended).
2. In the chemiSOLO’s Settings menu, navigate to the Software tab.
3. Under External Network Connection, fill in the Network Name and Network Password of the external network to connect the chemiSOLO’s Wi-Fi to.
4. Select Save, then restart the chemiSOLO with the Wi-Fi dongle accessory [CS1006] attached to one of the USB ports on the back of the unit by turning the power switch off and back on. This will implement the change and allow for internet access while connected to the chemiSOLO’s Wi-Fi.

Direct Ethernet Setup
Enabling internet access while connected to the chemiSOLO’s Wi-Fi can also be accomplished through direct ethernet connection with an active network modem. To set up the chemiSOLO this way, follow the steps below:

1. Connect the chemiSOLO Ethernet Connector [CS1007] to the chemiSOLO Ethernet to USB Adapter [CS1008].
2. Connect the ethernet side of the cord to the desired external internet modem.
3. Turn the chemiSOLO off, then connect the USB side of the cord to one of the two USB ports on the back of the chemiSOLO unit.
4. Turn the chemiSOLO back on and the connection will be complete. With this connection setup, users will have internet access while connected to the chemiSOLO’s Wi-Fi.

Image Capture Overview

The chemiSOLO captures high quality images with an intuitive user interface. The Home Screen expedites the imaging process by providing quick access to the most commonly-used functions. For users that require more specific settings, the Custom Imaging menu also allows for a comprehensive breakdown of the chemiSOLO’s functionalities.

Home Screen Overview
The Home Screen contains the following imaging options:

• Chemiluminescent Imaging – for samples with luminescent and chemiluminescent signals, such as ECL-HRP. See section 3.2 for additional information.
• Visible Imaging – for samples with visible stains and signals such as Coomassie dye in protein gels, film, or colorimetric-dyed blots. See section 3.3 for additional information.
• Custom Imaging – for full customization of imaging options. See section 3.4 for additional information.
• Auto-Save – automatically save images once they are taken. See section 3.5 for additional information.

Along the top, the Navigation Bar displays access to the following options:

Chemiluminescent Imaging
Use this function with samples that have luminescent and chemiluminescent signals such as ECL-HRP.

1. Place sample face-down on the imaging stage.

2. Fully close the chemiSOLO lid.

3. From the Home Screen, select the desired quick access option(s) in the Chemiluminescent Imaging section. If none of the following options are selected, the chemiSOLO will take a single chemi image with no marker when START is selected:

   • a. Color Marker – Select the Color Marker icon to take a true color image in addition to a chemiluminescent image. This function cannot be used at the same time as the Grayscale Marker function.

   • b. Grayscale Marker – Select the Grayscale Marker icon to take a grayscale image in addition
     to a chemiluminescent image. This function cannot be used at the same time as the Color Marker function.

   • c. Cumulative Imaging – Select Cumulative Imaging to take a series of cumulative chemiluminescent images. The number of images can be specified in the General tab of Settings under Chemi Image Mode, with the default setting being two images. This function can be used with either marker function active or with neither of them selected.

4. Select START to begin chemiluminescent image acquisition with an automatically-determined exposure time and 3×3 pixel binning.

   • a. If different settings are desired, refer to the Custom Options menu in Section 3.4.

5. After capture, the image(s) will appear in the Gallery automatically. The Gallery can also be reached by pressing the Gallery icon.

Visible Imaging
Use this function for samples with visible stains and signals such as Coomassie-dyed protein gels, film, or colorimetric-dyed blots.

1. Place sample face-down on the imaging stage.
2. Fully close the chemiSOLO lid.
3. From the Home Screen, select either:
   • a. C olor Imaging – The chemiSOLO will merge a composite image using individual red, blue, and green images for a full color image.
   • b. Grayscale Imaging – The chemiSOLO will take a single image using white light for a black-and-white image.
4.  Select START to begin visible image acquisition with automatically-determined exposure time(s) and 1×1 pixel binning.
   • a. If different settings are desired, refer to the Custom Options menu in Section 3.4.
5. After capture, the image will appear in the Gallery automatically. The Gallery can also be reached at any time by pressing the Gallery icon.

Custom Imaging
The Custom Imaging menu gives access to the Custom Options menu, which allows for full user customization of image acquisition.

The Custom Imaging menu allows for full user customization of image acquisition.

1. Place sample face-down on the imaging stage.
2. Fully close the chemiSOLO lid.
3. From the Home Screen, select the “Select Custom Options” button to access the Custom Imaging menu.
4. Specify the desired settings in each of the following fields:
   • a. Application – Choose between Chemi Imaging, True Color Imaging, or Grayscale Imaging
   • b. Sample – Specify whether the sample is Opaque, Translucent, or just select Auto-Detect for the chemiSOLO to determine it.
   • c. Pixel Binning – Select the desired pixel binning between 1×1 (unbinned), 2×2, 3×3, or 4×4 binning.
5. If Chemi Imaging was selected under Application, specify the following Chemi settings as well:
   • a. Marker – Choose between True Color Marker, Grayscale Marker, or No Marker
   • b. Imaging Mode – Choose between Single, Cumulative, or Multiple. These only apply to the chemiluminescent image; if a marker is selected, it will only be taken once.
   • c. Exposure Type – Choose between RapidCapture, Extended Dynamic Range, Overexposure, or Manual
   • d. Manual Exposure Time – If Manual is selected under Exposure Type, input a specific exposure time
6.  Select START to begin image acquisition according to the specified settings.
7. After capture, the image(s) will appear in the Gallery automatically. The Gallery can also be reached by pressing the Gallery icon.

Auto-Save
When Auto-Save is enabled, images taken will be automatically saved in a zip file as both 16-bit tiffs and 8-bit jpgs with the user-specified names and notes.

1. Enable auto-save by toggling it on the Home Screen.
2. Optional: Specify the image name and image notes that will be automatically saved with the next image.
3. Take an image as usual (see Sections 3.2, 3.3, and 3.4).
4. Each captured image will be automatically downloaded in a zip file containing the image as both a 16- bit tiff and an 8-bit jpg.

Image Gallery Overview

The Gallery allows for image viewing, editing, and information.

Gallery
Reach the Gallery by clicking the Gallery icon.

1. The following options are available within the File dropdown menu:
   • a. Open from chemiSOLO – Open an image stored on the chemiSOLO’s internal memory.
   • b. Close – Close the image that you’re currently viewing.
   • c. Close All – Close all images currently open in Gallery.
   • d. Save – Save the current image directly to the connected device. It will save through the Download pathway of the browser being used.
2. From the Print dropdown menu, you can:
   • a. Print – Print to a local printer connected to the system, or to a network printer.
   • b. P rint Report – Print the image you’re viewing as well as data about the image. These include Exposure type, Bin Level, Gain, Filter, Calibration, Software Version, System SN.
3. Select the Save to Device button to bring up the following popup:
   • a. Select whether to save your image(s) as a 16-bit tiff, 8-bit jpg, or both.
   • b. Press SAVE to save the currently-selected image in the specified formats or press SAVE ALL to save all images open in the chemiSOLO Gallery in the specified formats.
4. Select the Save to USB button to bring up the following popup:
   • a. Select whether to save your image(s) as a 16-bit tiff, 8-bit jpg, or both.
   • b. Press SAVE to save the currently-selected image in the specified formats or press SAVE ALL to save all images open in the chemiSOLO Gallery in the specified formats.
5. Select the Eject USB button to safely eject the connected USB memory drive from the chemiSOLO.

Image Adjustment Functions

1. Adjustments

   • a. Adjust the black, white and gamma levels of the image.
   • b. Clicking Auto will automatically adjust levels.
   • c. Click Invert to invert the colors of the signal and background.
   • d. Saturation will highlight in pink which pixels are beyond the detection limit of the camera.
   • e. For a chemi image with either color or gray marker, select the Hide Marker button to remove the marker from the display. Deselect the same button to display the marker again.
   • f. Note : Adjusting these settings will not affect the raw data when saved as a .tiff file. Only images saved as a .jpg will display with the adjusted contrast settings, as the .jpg file format alters the raw data. Azure recommends using .tiff formats for images that need to be quantified, and saving a copy as a .jpg with contrast adjustments for publication purposes.

2. ROI – Select of region of interest for further actions including Crop and Copy.
   a. Select:

   • i. Crop in order to isolate a particular area of the image. A new tab will open with an image of the selected area.
   • ii. Copy a region of interest on a single channel image. After selecting another image of the same size, use the paste option to overlay the copied image.
   • iii. Clear clipboard erases the saved image done with cropping or copying so you can select a new region.

3. Image Orientation – Rotate or flip images using the options provided. The free rotate function is helpful for images samples that were not imaged straight.

4. Resize – Customize width, height, and dots per inch (DPI).

5. Info – Gives detailed information about the active image. Displays the parameters for image acquisition including date & time, capture type, bin level, software version, and comments.

Settings Overview

The Settings menu allows for specific customization of the chemiSOLO imaging and operation settings. It can be reached by clicking the Settings option on the top ribbon.

General

1. File Saving – These settings allow you to enable the automatic saving of publication images.
   • a. Note: Publication images are for publication and printing purposes only and should not be used for data analysis.
2. Chemi Image Mode
   • a. The Number of Cumulative Images setting changes the number of images taken during a Cumulative capture. The default is two images and cannot exceed 10 images.
   • b. Check Auto Merge Chemi Marker Image to enable automatic merging of chemi and marker images upon capture.
   • c. RapidCapture Auto-exposure Ceiling – will calculate an optimal exposure time based on the signal ceiling entered set here. The ceiling range is 0 to 65,535 and the default setting is 35,000.

Software

1. chemiSOLO Wi-Fi Customization – Set a custom name and/or password for the chemiSOLO’s Wi-Fi.
   • a. Enter the desired Wi-Fi Name and Wi-Fi Password.
   • b. If no password is desired, leave the Wi-Fi Password section empty.
   • c. Select SAVE to finalize the chemiSOLO unit’s Wi-Fi settings.
   • d. Restart the chemiSOLO by turning the power off and on to implement the changed name and/or password.
2. External Network Connection – Connect the chemiSOLO’s Wi-Fi to an external network through the Wi-Fi dongle accessory.
   • a. Note: The chemiSOLO’s Wi-Fi can only be connected to an external network through this menu if the Wi-Fi dongle accessory is plugged in.
   • b. Connect the Wi-Fi dongle accessory to one of the USB ports in the center-bottom of the back of the unit.
   • c. Input the name and password of the desired external network.
   • d. Select SAVE to finalize the external network connection and save the network’s settings.
   • e. Restart the chemiSOLO by turning the power off and on to enable the network’s Wi-Fi access.
3. Image Memory – Show the image storage of the chemiSOLO’s internal memory.
   • a. The memory bar visualizes the amount of the chemiSOLO’s internal memory taken up by previously-taken images.
   • b. Note: It is highly recommended to always save images to an external memory source instead of relying on the chemiSOLO’s internal memory.
   • c. When the chemiSOLO’s internal memory is around or over 75% full, use the Clear Images button to delete all saved images from unit’s internal memory.
   • d. A prompt will appear to confirm all desired images have been saved to another location. Select Continue to clear all images from the chemiSOLO’s memory or Cancel to prevent the memory clear.
4. Software Upgrade – Update the chemiSOLO’s software to a new version.
   • a. Download the desired chemiSOLO software upgrade zip file to your external device (computer recommended) before connecting the device to the chemiSOLO.
   • b. Use the Browse button under the Software Upgrade section to select the chemiSOLO upgrade zip file without unzipping the contents.
   • c. Once the upgrade zip file is selected, press Submit to update the chemiSOLO’s internal software.
   • d. When the upgrade is complete, the chemiSOLO will automatically restart itself, indicated by the blue, white, and green light pattern on the unit’s front.
   • e. Following the restart, reconnect to the chemiSOLO, open the page, and perform a hard refresh on the web browser by pressing Ctrl+F5 on PC or Command+R on Mac. Following this, the update will be fully implemented.
   • f. Note: Once the unit’s software has been updated, the chemiSOLO upgrade file can be removed from the connected device as the chemiSOLO’s software will have been independently updated.

Create Darkmasters, Create Flats
Create Darkmasters, Create Flats – Darkmasters and Flats are set at the time of manufacture. If you require assistance with any of these settings, please contact Azure Biosystems Technical Support at support@azurebiosystems.com.

www.azurebiosystems.com • info@azurebiosystems.com
Copyright © 2023 Azure Biosystems. All rights reserved. The Azure Biosystems logo and Azure Biosystems® are trademarks of the Company. All other trademarks, service marks and trade names appearing in this brochure are the property of their respective owners.

References

• Life Science Instruments and Reagents - Azure Biosystems
• Warranty - Azure Biosystems

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