BRESSER NIB600 Series Professional Inverted Microscopes Instruction Manual

MANUAL INSTRUCTION
NIB600 SERIES
PROFESSIONAL INVERTED
MICROSCOPES

NIB600 Series Professional Inverted Microscopes

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NIB610
MANUAL INVERTED MICROSCOPE| NIB620
AUTOMATIC INVERTED MICROSCOPE| NIB630
AUTOMATIC INVERTED MICROSCOPE PRO

INTRODUCTION

WE CONGRATULATE YOU
on your purchase of a professional, inverted research microscope from the NEXCOPE NIB600 SERIES. It has been carefully manufactured with materials of lasting value, and careful attention has been paid to function and reliability. The inverted design allows microscopy of cell cultures, plankton, microalgae up to aseptic manipulation. Cell sampling and operation can be performed in Clean Bench. Due to the long life of the LED light source and the infinity optical system, you obtain high-definition and high contrast wide viewing images.
The Nexcope NIB600 microscopes are very robust, but need a certain amount of attention and care to guarantee their longevity. We therefore recommend that you read this user manual carefully and keep it within reach for your reference while working with the microscope. It contains all relevant information on operation, user safety and care. If you follow the guidelines, your microscope will function reliably and smoothly even after years of intensive use.
We wish you much success in your work with your new microscope!
The operating instructions can be downloaded from the following web address:

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https://www.bresser.de/PNX40610| https://www.bresser.de/PNX40620| https://www.bresser.de/PNX40630

WARRANTY TERMS
Visit our website via the following QR Code or web link to find further information on this product or the available translations of these instructions.

https://www.bresser.de/Mikroskopie/Mikroskope

WARRANTY

www.bresser.de/warranty_terms

We recommend that you study this manual thoroughly before operating the microscope for the first time. It will help you become fully familiar with the equipment and achieve optimum performance. Keep this manual in an easily accessible place near the work area for future reference.
Changes in the interest of the technical development are reserved. These instruction manual is not subject to the revision service. The reproduction of this document as well as the use and communication of its contents are not permitted unless expressly permitted. Contraventions obligate to compensation for damages. All rights in the event of a patent being granted or utility model registration reserved.

SERVICE
You can obtain additional information from your customer service:
Please contact the service centre first for any questions regarding the product or claims, preferably by e-mail.
E-Mail: [email protected]
Telephone: +44 1342 837 098
BRESSER UK Ltd.
Suite G3, Eden House
Enterprise Way
Edenbridge, Kent TN8 6HF
United Kingdom
Number charged at local rates in the UK (the amount you will be charged per phone call will depend on the tariff of your phone provider); calls from abroad will involve higher costs.

IMPORTANT NOTES

This chapter informs the user about the general instructions for the microscope and the important safety symbols.
1.1. Imprint/validity information
Imprint
Bresser GmbH
Gutenbergstraße 2
46414 Rhede Deutschland
http://www.bresser.de
For information regarding liability claims or service requests, please refer to chapters “Warranty” and “Service” in this documentation. Errors reserved – technical specifications subject to change.
Copyright: 2023 Bresser GmbH
All rights reserved.
It is not allowed to reproduce this documentation or parts of it in any kind (e.g. photocopying, printing, etc.) without written a permission of the manufacturer or the distributor, Bresser GmbH. It is also prohibited to use or transmit this documentation with electronic systems (e.g. image file, website, etc.). The descriptions and brand names of the corresponding firms used in this documentation are generally protected under trademark, brand and/or patent law for Germany, for the European Union and/or other countries.
Validity information
This documentation is valid for the products with the article numbers listed below: NIB610, NIB620, NIB630
Manual version: v012023b
Manual description: Manual_NIB-600-Series_en_NEXCOPE_v012023b Inquiries, please state these information.
1.2. About this instruction manual/intended use
About this instruction manual
These operating instructions are to be considered a component of the device.
PLEASE READ THE SAFETY INSTRUCTIONS AND THE OPERATING INSTRUCTIONS CAREFULLY BEFORE USE. Keep these instructions available for further reference when using the microscope. When the device is sold or given to someone else, the instruction manual must be provided to the new owner/ user of the product.
Intended use

• This product is intended for private and corporate use.
• It was developed for the magnified display of things in nature.
• The device is intended only for indoor use.

This device is not intended for use by individuals (including children) with limited physical, sensory or mental capabilities or those lacking in experience and/or knowledge, unless they are supervised by an individual responsible for their safety or have received instructions from them regarding the use of the device.
1.3. General safety instructions
Danger of an electric shock!
This device contains electronic components that operate via a power source (power supply and/or batteries). In case of any improper use of this device, there is a risk of an electric shock. An electric shock can cause severe injury or even death. Therefore please read the safety instructions below to avoid an explosion.

• Disconnect the device from the power supply by pulling the power plug when it is not used or in case of longer interruption of operation and before starting any work on maintenance and cleaning.

• Position your device so that it can be disconnected from the power supply at any time.
  The wall socket you use should be located near the device and easily accessible, since the plug on the power cable serves as a disconnecting device for the power supply.

• Always pull on the plug to seperate the device from the power supply. Never pull on the cable.

• Before operating, check the device, cables and connections for dammage.

• Never use a damaged unit or a unit with damaged power cables. Damaged parts must be exchanged immediately by an authorised service centre.

• Only use the device in complete dry environment and do not touch it with wet or moist parts of your body.

• The microscope is equipped with a plug-in power supply unit which allows the use of mains voltage values in the range of 100 to 240 V, 50 / 60 Hz, without additional voltage adjustment on the device.

• To avoid electric shock, connect the supplied power cord to a properly grounded power outlet on. These power cords have three-pin plugs to ensure proper grounding.

Danger of choking!
In case of any improper use of this device, there is a risk of choking, especially for children.
Therefore please read the safety instructions below.

• Keep packaging material, like plastic bags and rubber bans, out of the reach for children, as these materials pose a choking hazard!

Danger of explosion!
In case of any improper use of this device, there is a risk of an explosion. Therefore please read the safety instructions below to avoid and explosion.

• Do not expose the device to high temperatures. Use only the supplied power adapter.
  Do not short-circuit the device or throw them into a fire. Excessive heat or improper handling could trigger a short-circuit, a fire or an explosion.

• Do not use the microscope and the accessories supplied with them in potentially explosive atmospheres, in the presence of flammable solvents such as alcohol, petrol or volatile anaesthetics, etc..

CAUTION: Danger of injury!
This device contains components and/or accessories that can cause minor to severe injuries in case of any proper use. Therefore please read the safety instructions below to avoid any bodily injury.

• Tools with sharp edges and points are often used when working with this device. Because there is a risk of injury from such tools, store this device and all the tools and accessories in a location that is out of the reach of children.
• Children must not have access to the included chemicals and liquids. Do not drink the chemicals. Wash hands thoroughly with running water after using the chemicals. In the event that the chemicals come into contact with your eyes or mouth, rinse thoroughly with water. If you are in pain after exposure, contact a doctor immediately and take the substances with which you came into contact with you.

CAUTION: Fire hazard!
In case of any improper use of this device, there is a risk of fire. Therefore please read the safety instructions below to avoid the initiation of burning.

• Never cover the ventilation slots or cooling fins of the device while using it or as long as it has not sufficiently cooled down!

NOTICE: Risk of property damage!
In case of any improper use of this device and/or its accessories, there is a risk of property damage. Therefore only use the device according to the safety instructions below.

• Do not disassemble the device. In the event of a defect, please contact your dealer. The dealer will contact the Service Centre and can send the device in to be repaired,if necessary.
• Do not expose this device to higher temperatures and protect it from water and high humidity.
• Protect the device from severe shocks!
• For this device only use accessories and spare parts that comply with the technical information.
• Always use the power cord supplied by Nexcope. If an unsuitable power cord is used, Nexcope can no longer guarantee the electrical functionality and safety of the microscope.
• Use these microscope and their original accessories only for the applications described in this manual.
• The manufacturer does not accept any liability for any other application, possibly also for individual assemblies or individual parts. This also applies to all repair and service work that is not carried out by authorised service personnel. Therefore all guarantee /warranty claims expire.
• Nexcope NIB600 microscopes are not equipped with any special device to protect against corrosive, toxic, potentially infectious or radioactive samples or other samples that are harmful to health. All legal requirements, in particular national regulations for
  accident prevention, must be observed when handling such samples.

UV PROTECTION
The compact body is with anti-UV coating and can be placed into the clean bench for sterilization under UV lamp.
If you have any complaints or queries please contact your national service centre by telephone. The address is included in these instructions.
1.4. Safety symbols used in the manual

Danger symbols

| Warning of a danger point
This symbol indicates information that must be read and observed.
Non-observance can lead to:
o Risk of injury
o Malfunctions or equipment damage
| Warning of dangerous electrical voltage
This symbol precedes information that must be read and observed.
Non-observance can lead to:
o Risk of injury
o Malfunctions or equipment damage
Warning symbol
| Warning symbol indicates a possible source of danger.
Failure to follow the warnings may result in injury to the user and/or damage to the microscope (including nearby objects).
Note symbol
| Important additional hint
Accompanying instructions serve to simplify operation and maintenance.

1.5. Safety symbols on the microscope
The following symbols are located on the microscope/accessories and should always be observed:

| The device manufacturer is legally obliged to take back defective devices for recycling.
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****| EC Declaration of Confirmity
Bresser GmbH has issued a “Declaration of Conformity” in accordance with applicable guidelines and corresponding standards.

1.6. Sustainability
The protection of our environment is close to our heart.
That is why this manual is printed on 100% recycled paper.
1.7. Check scope of delivery

• Open the packaging with care. Avoid fingerprints and sweat on the camera lens. Prevent the camera and some accessories from falling and being damaged.
• Remove all components from the packaging and check for completeness according to the delivery note. The microscope could be packaged in more than one carton depending on the model or accessories.
• Handle the microscope with care and always protect it against violent impact and vibrations.
• Keep the original packaging for possible longer storage or return of the device to the supplier in case a repair or revision is needed.

Never lift the microscope by the illumination unit, cross stage, viewing head or coarse/fine focusing knobs. This can damage the microscope!

• Always carry the microscope with both hands.
• Only transport the microscopes of the NIB600 series using the carrying handles provided.

1.8. Site location

• Select a suitable location before setting up the microscope.

• Place the microscope on a resistant surface away from strong sunlight, heat sources, high humidity, high dust exposure as well as strong vibration.
  Microscope usage environmental requirements:
  Do not expose the device to temperatures below +10°C or higher than +35°C! If the device is exposed to temperatures outside this range, this will cause irreparable damage to optical and/or mechanical parts which are not covered by warranty.
  Maximum relative humidity: 80% at 31°C, linear temperature down to 34°C is 70%, 60% at 37°C, 50% at 40°C.

• Place the microscope on a surface that meets the following criteria:
  › Flat
  › Vibration-free
  › Level
  › Hard surface and non-flammable
  › Chemical and mechanical resistant

• To avoid blocking the natural convection air for cooling, ensure at least 10 centimetres of space around the microscope, from the wall and other objects.

• Place the microscope so that you can disconnect it from the mains at any time.

• The mains cable must be freely accessible at all times, as the mains cable is intended as a disconnecting device from the mains.

• Make sure any residual moisture is fully eliminated before use.

• For use in warm and humid climates, all optical components of the microscope are already equipped with anti fungus protection.

• Grounding the microscope to avoid lightning strike

1.9. Cleaning/maintenance/transport
Disconnect the device from the power supply by pulling the power plug when it is not used or in case of longer interruption of operation and before starting any work on maintenance and cleaning.
Remove optical elements (e.g. objectives, eyepiece, etc.) before cleaning the microscope body.
Be sure that the instrument is dry before using.
If a bacterial solution or splashes of water get on the stage, objective or observation tube, unplug the microscope immediately. Then wipe off the solution or water to keep the microscope dry. Otherwise, the unit could be damaged.
Do not use organic solvents (e.g. alcohol, ether, acetone, xylol or other dilutions) to clean lacquered parts or plastic parts!
Because the solvent such as ether and alcohol are extremely flammable, do not operate power switch of various electrical equipment when using, and keep away from open fire, please ensure indoor ventilation.
To avoid damaging the electronics, do not use any cleaning fluid.
When using objects that present a potential risk of infection, all parts that have come into contact with the object must be thoroughly cleaned.
The revolving nosepiece and the coarse microfocusing mechanism are precisely designed. Please avoid dismantling the components. This affects the performance of the microscope.
Transport: Please remove all loose parts and the object to be observed from the stage. Only use the original packaging for transport.
Environmental requirements for microscope transport:
a) Ambient temperature range: -40°C to +70°C.
b) Relative humidity range: 10% to 100%.
c) Range of atmospheric pressure: 500 hpa to 1060 hpa
Cleaning microscope body / optical elements

• Only use a dry cloth to clean the exterior of the device.
• Disassemble optical elements (e.g. objective, eyepiece, etc.) before cleaning.
• Blow away loose dust from the lens surfaces first with a blower. Do not use compressed air as it can contain oil or chemical agents.
• Use soft, single use paper tissue impregnated with 30%-70% of pure alcohol (available in a pharmacy or drug store). Wipe the lens surface with it. Do not apply cleaning solution to the optical parts directly.

Do not wipe the lens surface using the same portion of a cloth or tissue more than once.
Decontaminating the microscope

• We recommend use 70% medical alcohol for normal disinfection of the microscope.

Using organic solvents might result in discoloration of the plastic parts.
Caution on disposal
If a sample comes into contact with a hazardous sample, follow the standard procedure of your laboratory.
1.10. Protection and storage

• Protect the device against dust and moisture.

• Avoid putting fingerprints and contaminations on any optical surfaces.

• Pull a dust protection cover over the microscope.
  Before covering the microscope, always check that the microscope is also switched off.

• Store it in a closed container at a dry and mould-free place.

• Store the microscope and the accessories in the relevant containers when they are not used for a longer time.

• It is recommended to store also objectives and eyepieces in closed containers with desiccant.

A dust protection cover is included in delivery.
Remember:
A well maintained microscope will keep its optical quality for years and thus maintain its value.
Environmental requirements for microscope storage:
a) Ambient temperature range: -20°C to +60°C.
b) Relative humidity range: up to 90% (no condensation).
c) Range of atmospheric pressure: 500 hpa to 1060 hpa.
1.11. Disposal
Dispose of the packaging materials properly, according to their type, such as paper or cardboard. Contact your local waste-disposal service or environmental authority for information on the proper disposal.
Do not dispose of electronic devices in the household garbage!
As per Directive 2002/96/EC of the European Parliament on waste electrical and electronic equipment and its adaptation into German law, used electronic devices must be collected separately and recycled in an environmentally friendly manner.
The device manufacturer is legally obliged to take back defective devices.

NIB600 series

2.1. Overview NIB600 series
This chapter supports you with information about the NIB600 series in general.
You need to differentiate between the models NIB610, NIB620, NIB630.

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NIB630 – Automatic Inverted Microscope PRO| NIB610 – Manual Inverted Microscope| NIB620 – Automatic Inverted Microscope

2.2. The state-of-the-art microscopes – Offer you…
ERGONOMIC AND COMFORTABLE..
Control mechanisms in low-hand position
(NIB610/NIB620/NIB630)
The frequently control mechanism are close to the user and in lowhand position -> operation more quickly and conveniently.

45° Inclined Viewing Head
(NIB610/NIB620)
Inclined viewing head makes the user to operate microscope in a comfortable position. Minimize muscle tension and discomfort caused by long working hours.
Detachable Condenser
(NIB610/NIB620)
When culture flask is used, the condenser can be removed to increase working distance. It is also suitable for multilayer culture flask.

Long-handle Mechanical Stage
(NIB610/NIB620/NIB630)
The user can make comfortable and smooth movement during the operation, thereby improving work efficiency and comfort.
Stable and easily fits inside a Clean Bench
(NIB610/NIB620/NIB630)
The volume and weight of the body is reduced as much as possible in principle of stability. The compact body is with anti-UV coating and can be placed into the Clean Bench for sterilization under UV lamp. The distance between the eye point to the operation button and the focusing knob of the NIB600 is relatively short, and the distance from the stage is far away. So you realize cell sampling and operation inside the Clean Bench and observing comfortably outside.

NEW TILTABLE LIGHTING UNIT FOR IMPROVED WORKFLOW
(NIB630)
The tiltable lighting unit also allows higher sample flasks to be inserted. This simplifies the workflow considerably.
ERGO VIEWING HEAD (ALTERABLE ANGLE)
(NIB630)
The height of the binocular part can be adjusted and thus individually adapted to the user. This leads to a more comfortable microscope observation.

KOEHLER ILLUMINATION
(NIB630)
Koehler illumination provides the best possible light guidance of the microscopy light. Thereby stray light is avoided and only the just visible area in the sample is illuminated. This protects sensitive living samples from excessive light irradiation.
HIGH BRIGHTNESS, LONG LIFETIME LED ILLUMINATION
(NIB610/NIB620/NIB630)
LED Illumination, Suitable for Various Observations. With a high brightness and long lifetime LED illumination system for both transmission and fluorescent lighting, proving even brightness and cool lighting.

| NIB610/NIB620/NIB630
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Transmitted| Bright field, phase contrast, emboss contrast

INTELLIGENT OPERATING SYSTEM
(NIB620/NIB630)
Objective Coding Converter. It can memorize the illumination brightness when using each objective. When different objectives are converted to each other, the light intensity is automatically adjusted to reduce visual fatigue and improve work efficiency.
Liquid Crystal Display (LCD). The LCD shows the usage status of the microscope, including magnification, light intensity, sleep mode and more.

NIB610/NIB620 MICROSCOPE

3.1. Product image NIB610 – Manual Inverted Microscope

Power Supply: AC 100-240V; 50/60Hz
Specification of fuse: 250 V

Basic Equipment

 1.  Eyepiece
2.  Viewing head (Inclined at 45°)
3.  Main body
4.  Green LED power light
5.  Illumination ON/OFF switch
6.  Carrying handle 1
7.  Emboss contrast slider/placeholder
8.  Tension adjustment ring
9.  Right coarse focusing knob
10.  Right fine focusing knob| 11.  X-axis knob
12. Y-axis knob
13. Mechanical stage
14. Stage plate holder with universal holder and microscope slide
15. Stage plate holder set screw
16. Condenser
17. Universal phase contrast slider
18. Condenser set screw
19. LED lamp chamber
20. Aperture diaphragm (condenser diaphragm) lever
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DEPOSITOR NIB610 – BACK/SIDE VIEW

Power Supply: AC 100-240V; 50/60Hz
Specification of fuse: 250 V

Basic Equipment

21. Type plate with important information
22. Carrying handle 2
23. Spare fuse
24. Mains in
25. Power switch
26. Objective| 27. Quintuple nosepiece
28. Left coarse focusing knob
29. Left fine focusing knob
30. Brightness control knob
31. Photo adapter holder
32. Switching rod for visual observation/photo
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DEPOSITOR NIB620 – FRONT/SIDE VIEW
3.2. Product image NIB620 – Automatic Inverted Microscope

Power Supply: AC 100-240V; 50/60Hz
Specification of fuse: 250 V

Automatic Design
1. Liquid Crystal Display (LCD)
1a. Selected objective
1b. Light intensity (percentage unit/bars)
1c. Transmitted light ON/OFF
Basic Equipment

2. Aperture diaphragm (condenser diaphragm) lever
3. Eyepiece
4. Viewing head (Inclined at 45°)
5. Main body
6. Green LED power light
7. Carrying handle 1
8. Emboss contrast slider/placeholder
9. Tension adjustment ring
10. Right coarse focusing knob
11. Right fine focusing knob
12. X-axis knob| 13. Y-axis knob
14. Mechanical stage
15. Stage plate holder with universal
holder and microscope slide
16. Stage plate holder set screw
17. Condenser
18. Universal phase contrast slider
19. Condenser set screw
20. LED lamp chamber
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DEPOSITOR NIB620 – BACK/SIDE VIEW

Power Supply: AC 100-240V; 50/60Hz
Specification of fuse: 250 V

AUTOMATISCHES DESIGN
27. Encoded quintuple nosepiece
Basic Equipment

21. Type plate with important information
22. Carrying handle 2
23. Spare fuses
24. Mains in
25. Power switch
26. Objective| 28. Left coarse focusing knob
29. Left fine focusing knob
30. Brightness control knob
31. Photo adapter holder
32. Switching rod for visual observation/photo
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3.3. Assembly of the NIB610/NIB620 microscope
The diagram below shows the sequence of assembly of the various modules.
The numbers indicate the order of assembly.

Illustration 001: Assembly of NIB610/NIB620.
When assembling the microscope, make sure that all parts are free of dust and dirt and avoid scratching any parts or touching glass surfaces.
Check input voltage: The input voltage and supply voltage indicated on the back of the microscope must be consistent, otherwise the microscope will be seriously damaged.
Use suitable hexagonal screwdrivers for the assembly and replacement of components. One hexagonal screwdriver is included in the scope of delivery.

3.3.1. Detailed assembly procedure
1. Attaching the condenser

Loosen the condenser set screw (I).Align the condenser (II) with the condenser holder (III).
The labelling points to the front, the condenser set screw is on the right.
Slide the condenser horizontally into the condenser holder. Fix the condenser by tightening the condenser set screw.
2. Inserting the phase contrast slider

Illustration 003: NIB610/NIB620 – Inserting the phase contrast slider.
With the universal phase contrast slider (I) phase contrast and bright field observation is possible. The slider matches with the 10x/20x/40x phase contrast objectives. Positions without ring diaphragm can be used for bright field observation.
Insert the phase contrast slider (I) into the corresponding slot of the condenser (II).
Make sure that the phase contrast slider is always fully aligned in the light path. An audible “click” indicates the phase contrast slider and the optical axis position are accurate.
You can insert the phase contrast slider from either side of the condenser.
3. Mounting the mechanical stage

Illustration 004: NIB610/NIB620 – Mounting the mechanical stage.
At the beginning, make sure that the ruler of the table (A) is not extended.
Slide the mechanical stage (I) over the mechanical stage holder (II) and align the stage with the markings (III). There are two set screws (IV) underneath the mechanical stage. Insert these screws into the recesses of the mechanical stage holder and screw the stage tight.
4. Attaching the objectives

Illustration 005: NIB610 – Attaching the objectives to the nosepiece.

Use the coarse focusing knob (I) to turn the quintuple nosepiece (II) to the lowest position.
Screw the 4x objective (III) into any free space on the nosepiece from above the stage.
Turn the nosepiece one unit clockwise by hand and repeat the procedure for the next higher magnification until all objectives are mounted.
The following objectives are included in delivery:
4x: infinity planachromatic objective (NIS60)
10x,20x,40x: planachromatic phase contrast objective (NIS60)
Protect the free opening on the objective nosepiece with the black protective cap (IV).
The objectives snap into place with an audible “click” when they are po- sitioned in the beam path. Only in this position does the lighting come on.
Before each use, check the front lens of the objective for dirt. The clos- er a contamination is to the object or a camera sensor, the greater its effect on the visual or recorded image. Therefore clean the objectives regularly.

Illustration 006: NIB620 – Attaching the objectives to the nosepiece.

Connect the cold-device plug (I) to the appropriate mains in (II) on the back of the microscope.
Insert the main power plug (III) into the power socket (IV).
Move power switch (V) to position I to turn on the device on the left side of the microscope.

Use the coarse focusing knob (VI) to turn the encoded quintuple objective nosepiece (VII) to the lowest position.
Turn the nosepiece by hand until the Liquid Crystal Display (LCD) shows the programmed position for the 4x objective (VIII).
Screw the 4x objective (IX) into the assigned free space on the nosepiece.
Repeat the procedure for the next higher objectives to occupy the programmed positions on the nosepiece until all objectives are mounted.

The sequence is defined so that the magnification increases continuously when turning clockwise.
The following objectives are included in delivery:
4x: infinity planachromatic objective (NIS60)
10x,20x,40x: planachromatic phase contrast objective (NIS60)
Protect the free opening on the objective nosepiece with the black protective cap (X).
The objectives snap into place with an audible “click” when they are po- sitioned in the beam path. Only in this position does the lighting come on.
Before each use, check the front lens of the objective for dirt. The clos- er a contamination is to the object or a camera sensor, the greater its effect on the visual or recorded image. Therefore clean the objectives regularly.
5. Inserting the eyepieces

Illustration 007: NIB610/NIB620 – Inserting the eyepieces.

Remove the black protective caps (I) from the eyepiece sleeves (II).
Press eyepieces (III) gently into the eyepiece sleeves.
6. Inserting the placeholder/emboss contrast slider

Illustration 008: NIB610/NIB620 – Inserting the placeholder/emboss contrast slider.

If not already placed, insert the placeholder or the emboss contrast slider into the appropriate slider slot (I).
Placeholder is included in delivery.
Emboss contrast slider is part of the kit for emboss contrast. The kit can be ordered as an accessory.
7.  OPTIONAL – Using the photo adapters

Illustration 009: NIB610/NIB620 – Using the photo adapters.

Remove the black protective cap (I) from the photo adapter holder (II) on the left side of the microscope by unscrewing the set screw (III) with a suitable hexagonal screwdriver.
Place the C-mount adapter (0,5x or 1x – depending on the camera type) in the port of the photo adapter holder.
Fix both elements by tightening the set screw.
1x C-mount adapter is included in delivery.
0.5x C-mount adapter is not included in delivery but you can order it as an accessory.
When installing the camera, please always make sure that you hold it firmly in place to protect it from damage due to dropping.
3.4. Start-Up and explanation of the components
3.4.1. Setting up power supply and commissioning
Setting up power supply

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Illustration 010: NIB610/NIB620 – Setting up power supply and commissioning.
Connect the cold-device plug (1) to a suitable mains in (2) on the back of the microscope.
Insert the main power plug (3) into the power socket (4).
Switch on the device by setting the power switch (5) to position I on the left side.
The green LED power light (6) on the front of the microscope indicates commissioning.
Make sure the supplied voltage matches the instruments specifications: 100-240 V, 50/60 Hz.
To avoid electric shock, connect the cold-device plug to a properly grounded power socket. These cold-device plug has three-pin plugs to ensure proper grounding.
Cables and cords are vulnerable when bent or twisted. Never subject them to excessive force.
3.4.2. Turn on and adjust the illumination
Turn on the illumination

Illustration 011: NIB610 – Turn on the illumination.

Press out the black illumination ON/OFF switch (1) to turn on the lighting.

Set transmitted light ON

Illustration 012: NIB620 – Adjust transmitted light.

PRESS and TURN the brightness control knob (2):
Liquid Crystal Display (LCD)
Transmitted light ON (3)
Transmitted light OFF

Adjust the illumination

Illustration 013 : NIB610/NIB620 – Adjust the illumination.

Turn the brightness control knob (2) in the direction of the arrow → the light intensity increases and vice versa.
Only NIB620
Last used light intensity (4) is shown as number (percentage unit) and in form of bars in the LCD.
Make sure that the optical path for the binocular part is set to 100 %, see chapter 3.4.9.

3.4.3. Setting the sample on the mechanical stage

Illustration 014: NIB610/NIB620 – Sample placement.

Attaching the following sample holders enables you to observe various types of specimens:
Universal holder (1)
Terasaki holder (2)
Petri dish holder/Slide holder (Φ54mm) (3)
Petri dish holder (Φ90mm) (4)
Universal holder is included in the scope of delivery. The other holders can be ordered as accessories.
Turn in the 4x objective on the nosepiece. Objectives with higher magnification may collide with the specimen during observation due to the short working distance.
Place the universal holder (1) in the stage plate holder (5) with the scale facing forward. Then carefully place the sample to be examined, e.g. a microscope slide (6), on the universal holder. With the help of the two adjusting screws (7) on the universal holder, you can fit the slide exactly.
The adjustment knobs (8, 9) are coaxial.
The X-axis knob (8) moves the plate holder along the X-axis.
The Y-axis knob (9) moves the plate holder along the Y-axis.
3.4.4. Observationtubus
Observation head inclined at 45°, interpupillary distance: 48mm – 75mm.
The binocular observation head can be rotated 360°.

Illustration 015: NIB610/NIB620 – Adjusting the interpupillary distance.

1. Adjusting the interpupillary distance
(distance between the eyepieces)
Look through the eyepieces (1) and adjust the interpupillary distance (2) so that the left and right fields of view are completely aligned.
The device can be adjusted to the interpupillary distance of the respective observer between 48mm and 75mm.
The small white dots (3) indicate the interpupillary distance on the scale.

Proper interpupillary distance is crucial to the comfort of the user. Note your interpupillary distance so that it can be quickly duplicated.

Illustration 016: NIB610/NIB620 – Diopter adjustment.

2. Diopter adjustment
Look through one of the eyepiece (1) and focus the image (eyepiece freely selectable). Then look through the other eyepiece with the other eye and turn only the diopter adjustment ring (4) on this eyepiece to focus the sample.
The diopter compensation is an adjustment possibility on both eyepieces and serves to compensate for near – or farsightedness (max. +/-8 dpt. difference between both eyes can be compensated). If the difference is higher, or if you suffer from astigmatism etc. we recommend observing with your prescribed glasses on.

Illustration 017: NIB610/NIB620 – Eyepieces with rubber cups.

Diopter compensation is possible on both eyepieces but is only per- formed on one eye if the difference is +/- 4 dpt. or lower.
3. Protection through rubber cups
Both eyepieces are suitable for spectacle wearers. They are protected with rubber cups (5) so that the user’s eyeglasses are not damaged when accidentally coming in contact with the eyepiece.
3.4.5. Focusing on the Specimen
Focus block

Illustration 018: NIB610/NIB620 – Sample placement.

Control mechanisms (1) are close to the user and in low-hand position → operation more quickly and conveniently, and reduce the fatigue caused by the long observation.
Focusing controls
To adjust the focus, rotate the focusing knobs (2,3) on the right and left sides of the microscope.
Coarse focusing knobs (3a, 3b):
Pre-focusing, large distance between specimen and objective.
Fine focusing knobs (2a, 2b):
The fine focus is used for image sharpness.
Fine and precise regulation of the distance between the specimen and the objective.

Correlation between focusing knobs and objectives
Familiarize yourself with the correlation between the direction of rotation of the focusing knobs and the vertical movement of the objectives.

Focusing mechanism
From the focal point, up to 7mm and down to 1.5mm, and the limit can be up to 18.5mm. Spin out the limit screw (3) for about 4mm trip to limit.
The traveling distance of the objective for each knob is as follows:

The following operations may cause the product to malfunction:

• Turning the left and right focusing knobs in opposite directions.
• Turning the coarse and fine focusing knobs beyond their limit.

Tension adjustment ring
The coarse focusing knob (1) on the right side of the microscope is equipped with a tension adjustment ring (2) for adjusting the tightness of the coarse focus knob. To tighten the coarse focusing knob, rotate the tension adjustment ring in the direction of the arrow. To loosen the knob the opposite direction.

If the tension adjustment ring is set too loosely, the nosepiece will lower under its own weight. This leads to a loss of sharpness during observation. Therefore, adjust the torque accordingly.
3.4.6. The Liquid Crystal Display (LCD) (only for NIB620)
The Liquid Crystal Display of NIB620 shows the different working modes of the microscope, including magnification, light intensity, SLEEP mode, and so on. NIB620 can store and automatically adjust the light intensity for each objective. This improves work efficiency and reduces visual fatigue.
The following symbols appear on the LCD of NIB620 when you start the microscope and the 10x objective is tilted in:

The selected objective (1) is displayed next to the blue dot.
Last used light intensity (2) is shown as number (percentage unit) and in form of bars.
Display for transmitted light switched ON or OFF (3):
PRESS and TURN the brightness control knob (4):

| Transmitted light ON
---|---
| Transmitted light OFF

Double click on the brightness control knob (4) → the display shows LOCK (5).
LOCK: This function means that you can set and lock a desired light intensity when using an objective with a certain magnification.
This prevents any change by another user.
When changing objectives, the system automatically switches to the light intensity of the corresponding magnification, but the brightness adjustment knob (4) can no longer be adjusted manually.
Double click again on the brightness control knob (4) → LOCK-display (5) disappears.
The light intensity can be individually adjusted using the brightness control knob.
For the other display symbols, refer to the explanation in figure A.

Set a time after which the light is automatically switched off (switch-off time):
Press the brightness control knob (4) for at least 3 seconds. The TIME-display (6) appears with hours (h) and minutes (min). The TIME-display flashes for setting. Set the desired switch-off time by turning the  brightness control knob (5-minute increments from 0 to 8 hours).

| Switch-off time is extended by 5minutes
---|---
| Switch-off time is shortened by 5minutes

After setting the switch-off time, release the brightness control knob. The TIME-display changes to SLEEP-display after flashing three more times. The switch-off time (for example 15 min) is now saved. The microscope switches the light off after 15 min. The display then changes to the STANDBY/SLEEP mode.
You can leave the ECO mode by pressing the brightness control knob for 3 seconds. Then the display changes to the START/WORKING mode and the TIME /SLEEP-display disappears.
For the other display symbols, refer to the explanation in figure A.
The switch-off time should be set otherwise the 0:00 display flashes continuously.

In STANDBY/SLEEP mode, the light is switched off to save energy and lamp life.
Press once or turn the brightness control knob (4) – both directions possible – to change from STANDBY/SLEEP mode to START/WORKING mode.
Press once the brightness control knob to change from START/WORKING mode to STANDBY/SLEEP mode.
3.4.7. Aperture diaphragm (condenser diaphragm)

The aperture diaphragm (condenser diaphragm) adjusts the numerical aperture of the illumination system. The correct setting of the aperture diaphragm is important for the resolution, brightness, contrast and depth of field of the microscope image.

Aperture diaphragm lever to the right: The aperture diaphragm is closed → reducing resolution and brightness, and increasing contrast and depth of field.
Aperture diaphragm lever to the left: The aperture diaphragm is open, which increases resolution and brightness and reduces contrast and depth of field.
The resolution, brightness, contrast and depth of field properties are interrelated and cannot be set independently.
Based on the specimen, adjust the aperture diaphragm accordingly.
Adjust the aperture diaphragm every time you switch the objective.
The aperture diaphragm must never be used to adjust the light intensity, only to regulate the contrast.
Adjusting the aperture diaphragm while observing the actual diaphragm image through the eyepiece sleeve.
Place the 10x objective into the optical path and focus the sample to be examined.
It is recommended to use the 10x objective for exact aperture diaphragm adjustment.
Remove one of the eyepieces (3).
Insert the centering telescope (CT) (4) in the eyepiece sleeve.
Rotate the eyepiece of the CT to adjust the focus.
This reveals the objective pupil plane (5) and the image of the aperture diaphragm (6).
In addition, a black phase ring of the 10x phase objective is visible. This does not interfere with the setting.
Move the aperture diaphragm lever (Fig.A/1) so that the size of the aperture diaphragm image is 70-80% of the size of the objective pupil plane. This setting gives a reasonable contrast and a favourable image.
After adjustment, remove the CT and reinsert the eyepiece.
3.4.8. Detachable condenser

When culture flask is used, the condenser (1) can be removed to increase working distance (2) from 75mm to 187mm. It is also suitable for multilayer culture flask.
3.4.9. Light path of the viewing head

Use an appropriate photo adapter (1) for a camera connection to take microscope pictures. Via the switching rod for visual observation/photo (2) the light can be directed either to the eyepieces of the binocular part (3) or to the attached camera.

Illustration 026: NIB610/NIB620 – Light intensity distribution ratios.
Two light path settings are possible:
Switching rod complete in the unit:
In the first position according to the table, all light goes to the eyepieces. The camera port receives no light.
Switching rod completely out of the unit:
In the second position according to the table, all light goes to the camera. The camera port receives all the light. This is especially helpful when using contrast techniques or observing moving / living samples as exposure times are the shortest.
3.4.10. Compact main body, suitable for Clean Bench

The body is compact, stable and suitable for Clean Bench:
Can be sterilized in the Clean Bench
On the premise of ensuring the effect of imaging, NIB610/NIB620 are with compact design. The volume and weight of the body is reduced as much as possible in principle of stability. The compact body is with anti-UV coating and can be placed into the Clean Bench for sterilization under UV lamp.
Cell sampling and operation can be performed in Clean Bench
The distance between the eye point to the operation button and the focusing knob is relatively short, and the distance from the stage is far away. It is available to make the viewing head and operating mechanism outside, and stage, objectives and sample inside. So realize cell sampling and operation inside and observing comfortably outside.
3.5. How do I set up my microscope correctly?
3.5.1. Basic settings for bright field microscopy (NIB610/NIB620)

Before you start…

1. Microscope is switched on
2. Only NIB620: Transmitted light ON
3. Switching rod for visual observation/ photo is set to 100% to binocular part (rod pushed in)
4. 10x objective is swivelled in
5. Turn on the illumination (Regulation via the brightness control knob)
6. 6 Only NIB610: Turn on the illumination ON/OFF switch

Fully open the aperture.
Set the aperture diaphragm lever (1) to position O (open).
For further explanations see chapter 3.4.7.
Set the universal phase contrast slider (2) to bright field position
Select a hollow position → position without ring diaphragm → bright field observation
For further explanations see chapter 3.3.1./ 2

Place the sample (1) to be analyzed in the universal holder (2).
Rotate the X-/Y-axis knobs (3) to place the observed portion of the sample into the optical path.
For further explanations see chapter 3.4.3. and 3.4.5.

Focus on the sample: Look into the eyepieces (1) and turn the coarse focusing knob (2) towards you until the image of the sample is in focus. Then turn the fine focusing knob (3) to adjust the focus precisely.
Diopter adjustment: Look through one of the eyepiece (1) and focus the image (eyepiece freely selectable). Then look through the other eyepiece with the other eye and turn only the diopter adjustment ring (4) on this eyepiece to focus the sample.
Adjust interpupillary distance: Look through the eyepieces (1) and adjust the interpupillary distance (5) so that the left and right fields of view are completely aligned.
For further explanations see chapter 3.4.4. and 3.4.5.

Aperture diaphragm adjustment:
Rotate the aperture diaphragm (condenser diaphragm) lever so that the size of the aperture diaphragm image (2) is 70% to 80% of the exit pupil of the objective (3) to be used.
Adjust the aperture diaphragm every time you change the objective.
With the help of the centering telescope (CT) you can visualize the aperture diaphragm.
For further explanations see chapter 3.4.7.

Sample observation
Rotate the brightness control knob (1) to adjust the brightness of the field of view.
Rotate the fine focusing knob (2) to focus on the sample.
When replacing and observing the sample always check the brightness and the focus – adjust them as necessary.
For further explanations see chapter 3.4.2. and 3.4.5.

NIB630

4.1. Product image NIB630 automatic inverted microscope PRO

Special Features
Ergonomic Design
2.  Ergo Viewing head (Alterable angle)
Koehler Illumiation
17. Condenser
19. Condenser up-down knob
22. Field diaphragm lever
23. Condenser centering screws
24. Aperture diaphragm lever PRO Equipment
20. Three filter holders
21.  Transmitted light path shutter lever| Automatic Design
4.   Liquid Crystal Display (LCD)
4a. Selected objective
4b.  Light intensity (percentage unit/bars)
4c.  Transmitted light ON/OFF| Basic Equipment
1. Eyepiece
3. Viewing head holding screw
5. Green LED power light
6. Carrying handle 1
7. Main body
8.  Emboss contrast slider/placeholder
9. Tension adjustment ring
10. Right coarse focusing knob
11. Right fine focusing knob
12. X-axis knob
13. Y-axis knob
14. Mechanical stage
15.  Stage plate holder with universal holder and microscope slide
16. Stage plate holder set screw
18. Universal phase contrast slider
25. Condenser set screw
---|---|---

NIB630

Special Features
PRO Equipment
26. Tiltable lighting unit
Automatic Design
33. Encoded quintuple nosepiece| Basic Equipment
27. Type plate with important information
28. Carrying handle 2
29. Spare fuses
30. Mains in
31. Power switch
32. Objective
34. Left coarse focusing knob
35. Left fine focusing knob
36. Brightness control knob
37. Changing lever for visual observation/photo
38. Photo adapter holder
---|---

4.2. Assembly of the NIB630 microscope
The diagram below shows the sequence of assembly of the various modules.
The numbers indicate the order of assembly.

Illustration 031: Assembly of NIB630.

When assembling the microscope, make sure that all parts are free of dust and dirt and avoid scratching any parts or touching glass surfaces.
Check input voltage: The input voltage and supply voltage indicated on the back of the microscope must be consistent, otherwise the microscope will be seriously damaged.
Use the four supplied hexagonal screwdrivers for the assembly and replacement of components.
4.2.1. Detailed assembly procedure
1. Attaching the condenser

Loosen the condenser set screw (I) at the condenser holder (II).
Align the condenser (III): The marking to the front. Slide the condenser horizontally along the dovetail groove into the condenser holder. Fix the condenser by tightening the condenser set screw.

2 Inserting the phase contrast slide.r

With the universal phase contrast slider (I) phase contrast and bright field observation is possible. The slider matches with the 10x/20x/40x phase contrast objectives. Positions without ring diaphragm can be used for bright field observation.
Insert the phase contrast slider (I) into the corresponding slot of the condenser (II). The label of the slider should face the user.
You can insert the phase contrast slider from either side of the condenser.
Make sure that the phase contrast slider is always fully aligned in the light path. An audible “click” indicates the phase contrast slider and the optical axis position are accurate.
3. Mounting the mechanical stage

Illustration 034 A/B: NIB630 – Mounting the mechanical stage.

Slide the mechanical stage (I) over the mechanical stage holder (II) and align the stage with the mark (III). There are two set screws (IV) underneath the mechanical stage. Insert these screws into the recesses of the mechanical stage holder and screw the stage tight.
At the beginning, make sure that the ruler of the table (A) is not extended.

4 Attaching the objectives

Connect the cold-device plug (I) to the appropriate mains in (II) on the back of the microscope.
Insert the main power plug (III) into the power socket (IV).
Move power switch (V) to position I to turn on the device on the left side of the microscope.
Use the coarse focusing knob (VI) to turn the encoded quintuple objective nosepiece (VII) to the lowest position.
Turn the nosepiece by hand until the Liquid Crystal Display (LCD) shows the programmed position for the 4x objective (VIII).
Screw the 4x objective (IX) into the assigned free space on the nosepiece.
Repeat the procedure for the next higher objectives to occupy the programmed positions on the nosepiece until all objectives are mounted.
The sequence is defined so that the magnification increases continuously when turning clockwise.
The following objectives are included in delivery:
4x: infinity planachromatic objective (NIS60)
10x,20x,40x: planachromatic phase contrast objective (NIS60)
Protect the free opening on the objective nosepiece with the black protective cap (X).

The objectives snap into place with an audible “click” when they are positioned in the beam path. Only in this position does the lighting come on.
Before each use, check the front lens of the objective for dirt. The closer a contamination is to the object or a camera sensor, the greater its effect on the visual or recorded image. Therefore clean the objectives regularly.

5 Attaching the ergo tilting binocular viewing head

Loosen the viewing head holding screw (I) and remove the black protective cap (II) from the port for the binocular viewing head (III).
Remove the black protective cap (II) from the tube lens (at the bottom of the binocular viewing head).
Make sure not to touch any optical lenses.
Place the ergo tilting binocular viewing head (IV) onto the microscope body so that the eyepieces are in front.
The angle of the binocular viewing  head can be adjusted according tothe posture and build of the user.

6 Inserting the eyepieces

Loosen the small set screws (I) on the eyepiece sleeves (II) using the smallest hexagonal screwdrivers provided. Remove the black protective caps (III) from the eyepiece sleeves.
Press the eyepieces (IV) into the eyepiece sleeves.
Fix the eyepieces with the help of the small set screws.

7 OPTIONAL: Inserting the placeholder/emboss contrast slider

If not yet preinstalled, insert the placeholder or the emboss contrast slider into the appropriate slider slot (I) on the microscope.

8 OPTIONAL: Using the 1x C-mount photo adapter

Remove the black protective cap (I) from the photo adapter holder (II) by unscrewing the two set screws (III) with a suitable hexagonal screwdriver.
Place the 1x C-mount adapter in the port of the photo adapter holder.
Fix both elements by tightening the set screws.

1x C-mount adapter is included in delivery.
When installing the camera, please always make sure that you hold it firmly in place to protect it from damage due to dropping.

4.3. Start-up and explanation of the components
4.3.1. Setting up power supply and commissioning

Connect the cold-device plug (1) to a suitable mains in (2) on the back of the microscope.
Insert the main power plug (3) into the power socket (4).
Switch on the device by setting the power switch (5) to position I on the left side.
The green LED power light (6) on the front of the microscope indicates commissioning.
Make sure the supplied voltage matches the instruments specifications: 100-240 V, 50/60 Hz.
To avoid electric shock, connect the cold-device plug to a properly grounded power socket. These colddevice plug has three-pin plugs to ensure proper grounding.
Cables and cords are vulnerable when bent or twisted. Never subject them to excessive force.

4.3.2. Turn on and adjust the illumination

Set transmitted light ON
PRESS and TURN the brightness control knob (1):
Liquid Crystal Display (LCD)
Transmitted light ON (2)
Transmitted light OFF

Adjust the illumination
Turn the brightness control knob (1) in the direction of the arrow → the light intensity increases and vice versa.
Last used light intensity (3) is shown as number (percentage unit) and in form of bars in the LCD.

Make sure that the optical path for the binocular part is set to 100 % (see chapter 4.3.9.).

4.3.3. Placing the sample on the mechanical stage

Attaching the following sample holders enables you to observe various types of samples:
Universal holder (1)
Terasaki holder (2)
Petri dish holder/Slide holder ( Φ 54mm) (3)
Petri dish holder ( Φ 90mm) (4)

Universal holder is included in the scope of delivery. The other holders can be ordered as accessories.
Before starting, check that the nosepiece is in the lowest position.
Turn in the 4x objective on the nosepiece.
Objectives with higher magnification may collide with the sample during sample observation due to the short working distance.

Place the universal holder (1) in the stage plate holder (5) with the scale facing forward. Then carefully place the sample to be examined, e.g. a microscope slide (6), on the universal holder.
With the help of the two adjusting screws (7) on the universal holder, you can fit the slide exactly.
The adjustment knobs (8, 9) are coaxial.
The X-axis knob (8) moves the plate holder along the X-axis.
The Y-axis knob (9) moves the plate holder along the Y-axis.

4.3.4. Observationtubus
Ergo tilting binocular viewing head alterable angle, interpupillary distance: 48mm to 75mm.
Observationtubus can be rotated 360°.

Adjusting the interpupillary distance (distance between the eyepieces)
Look through the eyepieces (1) and adjust the interpupillary distance (2) so that the left and right fields of view are completely aligned.
The device can be adjusted to the interpupillary distance of the respective observer between 48mm and 75mm.
The small white dots (3) indicate the interpupillary distance on the scale.
Proper interpupillary distance is cru- cial to the comfort of the user. Note your interpupillary distance so that it can be quickly duplicated.

Diopter adjustment
Look through one of the eyepiece (1) and focus the image (eyepiece freely selectable).
Then look through the other eyepiece with the other eye and turn only the diopter adjustment ring (4) on this eyepiece to focus the sample.

The diopter compensation is an adjustment possibility on both eyepieces and serves to compensate for nearor farsightedness (max. +/-8 dpt. difference between both eyes can be compensated). If the difference is higher, or if you suffer from astigmatism etc. we recommend observing with your prescribed glasses on.
Diopter compensation is possible on both eyepieces but is only performed on one eye if the difference is +/- 4 dpt. or lower.

Protection through rubber cups
Both eyepieces are suitable for spectacle wearers. They are protected with rubber cups (5) so that the user’s eyeglasses are not damaged when accidentally coming in contact with the eyepiece.

Adjusting the angle of the binocular part
You can adjust the angle of the binocular part according to your posture and physique.

4.3.5. Sample focusing
Focus block

Control mechanisms (1) are close to the user and in low-hand position → operation more quickly and conveniently, and reduce the fatigue caused by the long observation.
Focusing controls
To adjust the focus, rotate the focusing knobs (2,3) on the right and left sides of the microscope.
Coarse focusing knobs (2a, 2b):
Pre-focusing, large distance between specimen and objective.
Fine focusing knobs (3a, 3b):
The fine focus is used for image sharpness.
Fine and precise regulation of the distance between the specimen and the objective.

Correlation between focusing knobs and objectives
Familiarize yourself with the correlation between the direction of rotation of the focusing knobs and the vertical movement of the objectives.

Focusing mechanism
From the focal point, up to 7mm and down to 1.5mm, and the limit can be up to 18.5mm. Spin out the limit screw (3) for about 4mm trip to limit.
The traveling distance of the objective for each knob is as follows:

The following operations may cause the product to malfunction:

• Turning the left and right focusing knobs in opposite directions.
• Turning the coarse and fine focusing knobs beyond their limit.

Tension adjustment ring
The coarse focusing knob (1) on the right side of the microscope is equipped with a tension adjustment ring (2) for adjusting the tightness of the coarse focus knob. To tighten the coarse focusing knob, rotate the tension adjustment ring in the direction of the arrow.
To loosen the opposite direction.

If the tension adjustment ring is set too loosely, the nosepiece will lower under its own weight. This leads to a loss of sharpness during observation. Therefore, adjust the torque accordingly.

4.3.6. The Liquid Crystal Display (LCD)
The Liquid Crystal Display of NIB630 shows the different working modes of the microscope, including magnification, light intensity, SLEEP mode, and so on.NIB630 can store and automatically adjust the light intensity for each objective. This improves work efficiency and reduces visual fatigue.
The following symbols appear in the LCD of NIB630 when you start the microscope and the 10x objective is tilted in:

The selected objective (1) is displayed next to the blue dot.
Last used light intensity (2) is shown as number (percentage unit) and in form of bars.
Display for transmitted light switched ON or OFF (3):
PRESS and TURN the brightness control knob (4):

| Transmitted light ON
---|---
| Transmitted light OFF

Double click on the brightness control knob (4) → the display shows LOCK (5).
LOCK: This function means that you can set and lock a desired light intensity when using an objective with a certain magnification.
This prevents any change by another user.
When changing objectives, the system automatically switches to the light intensity of the corresponding magnification, but the brightness adjustment knob (4) can no longer be adjusted manually.
Double click again on the brightness control knob (4) → LOCK-display (5) disappears.
The light intensity can be individually adjusted using the brightness control knob.
For the other display symbols, refer to the explanation in figure A.

Set a time after which the light is automatically switched off (switch-off time):
Press the brightness control knob (4) for at least 3 seconds. The TIME-display (6) appears with hours (h) and minutes (min). The TIME-display flashes for setting.
Set the desired switch-off time by turning the brightness control knob (5-minute increments from 0 to 8 hours).

| Switch-off time is extended by 5minutes
---|---
| Switch-off time is shortened by 5minutes

After setting the switch-off time, release the brightness control knob. The TIME-display changes to SLEEP-display after flashing three more times. The switch-off time (for example 15 min) is now saved. The microscope switches the light off after 15 min.
The display then changes to the STANDBY/SLEEP mode.
You can leave the ECO mode by pressing the brightness control knob for 3 seconds. Then the display changes to the START/WORKING mode and the TIME /SLEEP-display disappears.

For the other display symbols, refer to the explanation in figure A.
The switch-off time should be set otherwise the 0:00 display flashes continuously.

In STANDBY/SLEEP mode, the light is switched off to save energy and lamp life.
Press once or turn the brightness control knob (4) – both directions possible – to change from STANDBY/SLEEP mode to START/WORKING mode.
Press once the brightness control knob to change from START/WORKING mode to STANDBY/SLEEP mode.

4.3.7. Transmitted light path shutter

There is a shutter in the transmitted light path. The shutter prevents bleaching of a sensitive sample when it is not under observation. Furthermore, the shutter protects the LED lamp from repeated switching on and off. You can open ( ) or close ( ) the shutter using the transmitted light path shutter lever (1).

4.3.8. Tiltable lightning unit

The tiltable lighting unit (1) allows higher sample flasks to be inserted without having to remove the condenser. This increases the working distance (2) and simplifies the workflow considerably.

4.3.9. Light path of the ergo tilting viewing head

To capture microscope images, mount the appropriate camera on the 1x C-mount photo adapter.
Via the changing lever for visual observation/photo (1) the light can be directed either to the eyepieces of the binocular part (2) or to the attached camera (3).

Illustration 050 B: NIB630 – Light intensity distribution ratios.

Two light path settings can be selected with the help of the changing lever for visual observation/photo:
Binocular part ← Icon
In this position according to the table, all light goes to the eyepieces. The camera port receives no light.
Binocular part/Camera port → Icon
In the second position, the eyepieces receive 20%, while the camera receives 80% of the light. Visual observation and photomicrography can be done simultaneously.

4.3.10. Main body suitable for Clean Bench

The body is compact, stable and suitable for a Clean Bench:
Can be sterilized in the Clean Bench
On the premise of ensuring the effect of imaging, NIB630 is with compact design.
The volume and weight of the body is reduced as much as possible in principle of stablity. The compact body is with anti-UV coating and can be placed into the Clean Bench for sterilization under UV lamp.

Cell sampling and operation can be performed in Clean Bench
The distance between the eye point to the operation button and the focusing knob is relatively short, and the distance from the stage is far away. It is available to make the viewing head and operating mechanism outside, and stage, objectives and sample inside. So realize cell sampling and operation inside and observing comfortably outside.

4.4. Bright field settings according to Koehler
4.4.1. Prerequisites for setting Koehler illumination

Condenser (1)

• Height adjustable via the condenser updown knob (2)
• Centreable via condenser centering screws (3)

Aperture diaphragm

• Adjustable via the aperture diaphragm lever (4)

Field diaphragm

• Adjustable via the field diaphragm lever (5)

Condenser task: filling the entire aperture (the opening width) of the objective with light in order to achieve the greatest possible resolution.
With the aperture diaphragm you can control the resolution, contrast and depth of field. After each change of objective, the aperture diaphragm must be readjusted.
When the illumination is set correctly, only the section of the sample being examined is illuminated by the field diaphragm. The sample is protected from excessive light irradiation and at the same time the formation of contrast- reducing stray light is minimised.
Always set the Koehler illumination on the NIB630 microscope with the sample carrier/sample bottle that will subsequently be used.

4.4.2. Setting the condenser

Before you start, check that:

• Changing lever for visual observation/photo is set to binocular part
• Universal phase contrast slider is set to bright field
• Transmitted light path is open
• No additional filters are swivelled into the beam path
• Object to be examined is placed on the stage

PRESS and TURN the brightness control knob (1) to turn transmitted light ON (2).
Place a 10x objective into the optical path and focus on the sample.
Turn the field diaphragm lever (3) all the way to the right to close the field diaphragm completely. Look through the eyepieces.
Turn the condenser up-down knob (4) to adjust the high of the condenser until the image of the field diaphragm shows a sharp outline.
If the condenser is out of center, you will see an image of the iris opening similar to (5a).
In order to center the iris opening in the field of view, use the two condenser centering screws (6) at each side of the condenser holder.
Once the image is in focus and centered in the field of view (5b), open the field diaphragm until the image is at least as large as field of view (5c-5d).

The field diaphragm restricts the diameter of the light beam entering the objective.
This prevents the incidence of disturbing light and increases the image contrast. To support objective performance, the diameter of the field diaphragm must be adjusted so that its image and the field of view are the same size.

4.4.3. Setting the aperture diaphragm (condenser diaphragm)

The aperture diaphragm (condenser diaphragm) adjusts the numerical aperture of the illumination system. The correct setting of the aperture diaphragm is important for the resolution, brightness, contrast and depth of field of the microscope image.

Before you start, check that:

• Changing lever for visual observation/photo is set to binocular part
• Universal phase contrast slider is set to bright field
• Transmitted light path is open
• No additional filters are swivelled into the beam path
• Object to be examined is placed on the stage

Aperture diaphragm lever (1) to the right:
The aperture diaphragm is closed → reducing resolution and brightness, and increasing contrast and depth of field.
Aperture diaphragm lever (1) to the left:
The aperture diaphragm is open, which increases resolution and brightness and reduces contrast and depth of field.

The resolution, brightness, contrast and depth of field properties are interrelated and cannot be set independently.
Based on the specimen, adjust the aperture diaphragm accordingly.
The aperture diaphragm must never be used to adjust the light intensity, only to regulate the contrast.

Adjusting the aperture diaphragm
Place the 10x objective into the optical path and focus on the sample.
Close the aperture diaphragm → aperture diaphragm lever (1) to the right.
Loosen the small screw on the eyepiece sleeve to remove one of the eyepieces (2).
Use a suitable hexagonal screwdriver.
Insert the centering telescope (CT) (3) in the eyepiece sleeve. Rotate the CT to adjust the focus. This reveals the objective pupil plane (4) and the image of the aperture diaphragm (5).
In addition, a black phase ring of the 10x phase objective is visible. This does not interfere with the setting.

Move the aperture diaphragm lever (1) to the left so that the size of the diaphragm image is 70-80% of the size of the objective pupil plane. This setting gives a reasonable contrast and a favourable image. After adjustment, remove the CT and reinsert the eyepiece.
Tighten the set screw again.
The centering telescope (CT) is included in the scope of delivery.
Each time the objective is changed, the object field size and objective aperture and possibly the centering will change slightly, so that for optimal results, the light field and aperture diaphragm settings should be adjusted again.

4.5. How do I set up my microscope correctly?
4.5.1. Bright field settings according to Koehler (NIB630)

Before you start…

1. Microscope is switched on
2. Transmitted light ON (press and turn the brightness control knob)
3. Changing lever for visual observation/photo is set to 100% to binocular part
4. Transmitted light path shutter is open
5. No additional filters are swivelled into the beam path
6. 10x objective is swivelled in
7. Turn on the illumination (Regulation via the brightness control knob)

Set the universal phase contrast slider (1) to bright field position
Select a hollow position → position without ring diaphragm → bright field observation

For further explanations see chapter 4.2.1./ 2

Place the sample (1) to be analyzed in the universal holder (2).
Rotate the X-/Y-axis knobs (3) to place the observed portion of the sample into the optical path

For further explanations see chapter 4.3.3.

Focus on the sample: Look into the eyepieces (1) and turn the coarse focusing knob (2) until the image of the sample is in focus.
Then turn the fine focusing knob (3) to adjust the focus precisely.
Diopter adjustment:
Look through one of the eyepiece (1) and focus the image (eyepiece freely selectable). Then look through the other eyepiece with the other eye and turn only the diopter adjustment ring (4) on this eyepiece to focus the sample.
Adjust interpupillary distance:
Look through the eyepieces (1) and adjust the interpupillary distance (5) so that the left and right fields of view are completely aligned.

For further explanations, see chapter 4.3.4. and 4.3.5.

Centering the condenser
Turn the field diaphragm lever (1) all the way to the right to close the field diaphragm completely. Look through the eyepieces (2).
Turn the condenser up-down knob (3) to adjust the height of the condenser so that the image of the field diaphragm shows a sharp outline.
If the condenser is out of center, you will see an image of the iris opening similar to (4a).
In order to center the iris opening in the field of view, use the two condenser centering screws (5) at each side of the condenser holder.
Once the image is in focus and centered in the field of view (4b), open the field diaphragm until the image is at least as large as field of view (4c-4d).

For further explanations see chapter 4.4.2.

Aperture diaphragm (condenser diaphragm)
Aperture diaphragm lever (1) to the right: The aperture diaphragm is closed → reducing resolution and brightness, and increasing contrast and depth of field.
Aperture diaphragm lever (1) to the left: The aperture diaphragm is open, which increases resolution and brightness and reduces contrast and depth of field.

Adjusting the aperture diaphragm

Check that the 10x objective is placed in the beam path.
Close the aperture diaphragm → aperture diaphragm lever (1) to the right.
Loosen the small screw on the eyepiece sleeve to remove one of the eyepieces (2).

Use a suitable hexagonal screwdriver
Insert the centering telescope (CT) (3) in the eyepiece sleeve. Rotate the CT to adjust the focus. This reveals the objective pupil plane (4) and the image of the aperture diaphragm (5).
Move the aperture diaphragm lever (1) to the left so that the size of the diaphragm image (5) is 70-80% of the size of the objective pupil plane (4). This setting gives a reasonable contrast and a favourable image.
After adjustment, remove the CT and reinsert the eyepiece. Tighten the set screw again.

For further explanations, see chapter 4.4.3.

Sample observation
Rotate the brightness control knob (1) to adjust the brightness of the field of view.
Rotate the fine focusing knob (2) to focus on the sample.

When replacing and observing the sample always check the brightness and the focus – adjust them as necessary.
For further explanations, see chapter 4.3.2. and 4.3.5.

OBSERVATION METHODS

5.1. Bright field observation
NIB610/NIB620: Basic settings for bright field microscopy (see chapter 3.5.1.)
NIB630: Bright field settings according to Koehler (NIB630) (see chapter 4.5.1.)

5.2. Phase contrast observation
5.2.1. Overview
Phase contrast microscopy is used to convert invisible phase shifts into differences in brightness that are perceptible to our eyes. This effect is achieved by the interference of diffracted light from the object and direct microscopic light. The phase shift through the specimen is thus converted into a change in amplitude. This enables direct imaging of structures that have only a low inherent contrast and would only be visible with artificial coloring in bright field microscopy. These include, for example, plankton organisms or  activated sludge. Cell cultures or cells in the urine sediment can also be better visualized with phase contrast and thus be evaluated more quickly and reliably.

Illustration 056: Example images for phase contrast, source: NEXCOPE.

5.2.2 Components for phase contrast observation

All components are included in the scope of delivery.

• 3x Planachromatic phase contrast objectives
  (NIS60): 10x, 20x, 40x

• Universal phase contrast slider

Phase contrast and bright field observation is possible.
The slider matches with the 10x/20x/40x phase contrast objectives.

1. Hollow position → position without ring diaphragm → bright field observation
2. Ring diaphragm → phase contrast observation
3. At the side are centering screws

• Centering telescope (CT): serves for better centering

5.2.3. Settings for phase contrast observation
NIB610/NIB620

Before you start…

1. Microscope is switched on
2. Only NIB620: Transmitted light ON
3. Switching rod for visual observation/photo is set to the binocular part
4. Phase contrast slider is set to bright fild
5. 10x objective is swivelled in
6. Object is in focus

Set the phase contrast slider (1) to ring diaphragm 10/20/40.
Set the aperture diaphragm lever (2) to position O (open).

If the aperture diaphragm is closed, it hinders the ring diaphragm and phase contrast effects cannot be obtained.
Replace an eyepiece (3) with the centering telescope (CT) (4).
The CT enlarges the phase rings and makes centering easier. An approximate centering of the phase rings is also possible without CT.

Turn the CT until two sharp rings become visible.
Check the centering and overlap of the bright ring diaphragm (in the condenser) (5) with the dark phase ring (in the objective) (6).
Adjust the centering screws (7) on the phase contrast slider until the bright ring diaphragm and the dark phase ring completely overlap.

Use a suitable hexagonal screwdriver.
Perfect phase contrast can only be achieved when the bright ring diaphragm and the dark phase ring exactly covers the beam path.
Then switch to the next objective, check the phase rings for all objectives and adjust if necessary. After centering, remove the CT, insert the eyepiece and observe your sample in phase contrast.
When observing and replacing the specimen, check the focus and the brightness → adjust them as necessary.

5.2.3. Settings for phase contrast observation
NIB630

Before you start…

1. Microscope is switched on
2. Transmitted light ON
3. Changing lever for visual observation/ photo is set to binocular part
4. Use the transmitted light path shutter lever to open the shutter.
5. Phase contrast slider is set to bright field
6. 10x objective is swivelled in
7. Object is in focus → Unit is set in bright field according to Koehler

Illustration 058 A: NIB630 – Settings before you start.

Set the phase contrast slider (1) to ring  diaphragm 10/20/40.Set the aperture diaphragm lever (2) all the way to the left so that the aperture of the condenser is fully opened.
If the aperture diaphragm is closed, it hinders the ring diaphragm and phase contrast effects cannot be obtained.
Replace an eyepiece (3) with the centering telescope (CT) (4).

Illustration 058 B: NIB630 – Settings for phase contrast.

Use a suitable hexagonal screwdriver.
The CT enlarges the phase rings and makes centering easier. An approximate centering of the phase rings is also possible without CT.

Turn the CT until two sharp rings become visible. Check the centering and overlap of the bright ring diaphragm (in the condenser) (5) with the dark phase ring (in the objective) (6).
Adjust the centering screws (7) on the phase contrast slider until the bright ring diaphragm and the dark phase ring completely overlap.

Illustration 058 C: NIB630 – Settings for phase contrast.

Use a suitable hexagonal screwdriver.
Perfect phase contrast can only be achieved when the bright ring diaphragm and the dark phase ring exactly covers the beam path.
Then switch to the next objective, check the phase rings for all objectives and adjust if necessary.
After centering, remove the CT, insert the eyepiece and observe your sample in phase contrast.
When observing and replacing the specimen, check the focus and the brightness → adjust them as necessary.

5.3. Emboss contrast observation (Relief 3D contrast)
5.3.1. Overview
Emboss contrast is a newly developed contrast observation method which is easy to operate. Even without extra optical components, pseudo-three dimensional and glare-free image can be optained just through adding adjustment slider. This technique allows the observation of thick cells such as embryos or iPS cells (induced pluripotent stem cells) that would normally suffer from halos in conventional phase contrast methods. Emboss contrast is compartible with glass and plastic culture dishes.

5.3.2. Oblique illumination
The emboss contrast observation is based on the method of oblique illumination. Here, a gain in contrast is achieved by covering part or half of the normal illumination beam path. The condenser-side emboss contrast slider is equipped with a sector diaphragm.
This blocks part of the light. The specimen is then illuminated at an angle, resulting in a shadow cast on the fine structures of the specimen. The eyepiece-tube-side emboss contrast slider serves as a ring diaphragm. This cuts the light beam out of the light cone.

5.3.3. Components for emboss contrast observation
All components for emboss contrast observation are not included in the scope of delivery. This set can be purchased as an accessory.

The emboss contrast observation set consists of two emboss contrast sliders:

Condenser-side emboss contrast slider
The slider matches with the 10x/20x/40x phase contrast objectives.

• The slider is equipped with a sector – diaphragm (1)
  → The specimen is then illuminated at an angle

• Green filter (ND6 filter) (2)
  → Suppresses disturbing color fringes on the object contours

• Hollow positions (3) → position without ring diaphragm → bright field observation

Illustration 059 A: NIB610/NIB620/NIB630
Condenser-side emboss contrast slider.

Eyepiece-tube-side emboss contrast slider
The slider has position markings for emboss contrast microscopy.
→ serves as a ring diaphragm; this cuts the light beam out of the light cone; sharp edges are created

Illustration 059 B: NIB610/NIB620/NIB630 –
Eyepiece-tube-side emboss contrast slider.

5.3.4. Settings for emboss contrast observation
NIB610/NIB620
Bright field position marking

Before you start…

1. Microscope is switched on
2. Only NIB620: Transmitted light ON
3. Switching rod for visual observation/photo is set to 100% to binocular part
4. 10x objective is swivelled in
5. Object is in focus
   → Focus on the specimen under bright field microscopy conditions (see chapter 3.5.)

Illustration 060 A: NIB610/NIB620 – Settings before you start.

Settings
Insert the condenser-side emboss contrast slider into the slot provided on the condenser.
Set the slider to the sector diaphragm marked 10-20-40.

Start without the green filter. This can simply be inserted after the basic setting.
Remove the placeholder (2) from the right side of the eyepiece tube. Insert the eyepiece-tube-side emboss contrast slider (3) into the beam path.

Attaching the centering telescope (CT) to the eyepiece tube enables you to view the sector diaphragm image.

OBSERVATION
Set the aperture diaphragm lever (4) to position O (open).
If the aperture diaphragm is closed, it hinders the sector diaphragm of the condenser-side emboss contrast slider and emboss contrast effects cannot be obtained.
When replacing and observing the specimen, check the focus and the brightness, and adjust them as necessary.

Illustration 060 C: NIB610/NIB620 – Observation with emboss contrast.

5.3.5. Settings for emboss contrast observation
NIB630

Before you start…

1. Microscope is switched on
2. Transmitted light ON
3. Changing lever for visual observation/ photo is set to 100% to binocular part
4. Use the transmitted light path shutter lever to open the shutter.
5. 10x objective is swivelled in
6. Object is in focus
   → Unit is set in bright field according to Koehler (see chapter 4.5.)

Illustration 061 A: NIB630 – Settings before you start.

Settings
Insert the condenser-side emboss contrast slider (1) into the slot provided on the condenser.
Set the slider to the sector diaphragm marked 10-20-40.

• Attaching a CT to the eyepiece tube enables you to view the sector diaphragm image.
• Start without the green filter. This can simply be inserted after the basic setting.
• Check that there are no additional filters in the beam path (2).

Remove the placeholder (3) from the right side of the eyepiece tube. Insert the eyepiecetube-side emboss contrast slider (4) into the beam path. The slider should snap into place at the position of the same number as the magnification of the objective.

OBSERVATION
Set the aperture diaphragm lever (5) all the way to the left so that the aperture of the condenser is fully opened.

If the aperture diaphragm is closed, it hinders the sector diaphragm of the condenser-side emboss contrast slider and emboss contrast effects cannot be obtained.
When replacing and observing the specimen, check the focus and the brightness, and adjust them as necessary.

EQUIPMENT OVERVIEW

6.1. Main technical specifications of NIB610/NIB620

4x,10x/20x, 40x, 60x
(for semi-apochromatic contrast)| o| 0
Emboss contrast slider| contrast slider| o| o
ECO| | o| —
Camera mount| C-Mount lx| •| •
C-Mount 0.5x| o| 0
LCD| | —| •

Note: ● Standard component, ○ Optional, ― Not offered

6.2. Scope of delivery NIB610/NIB620
NIB610/NIB620 Inverted microscope with quintuple nosepiece

• 4x objectives (10x, 20x, 40x with phase contrast), 4x as bright field
• 2x Extra wide field eyepieces 10×/22
• 1x Universal holder
• 1x Universal phase contrast slider
• 1x centering telescope (CT)
• 1x C-mount photo adapter
• 2x spare fuses
• 1x Nexcope power cord
• 1x Hexagonal screwdriver
• 1x Dust cover

6.3. Main technical specifications of NIB630

Note: ● Standard component, ○ Optional, ― Not offered

6.4. Scope of delivery NIB630
NIB630 inverted microscope with intelligent quintuple nosepiece

• 4x objectives (10x, 20x, 40x with phase contrast), 4x as bright field
• 2x Extra wide field eyepieces 10×/22
• 1x Universal phase contrast slider
• 1x Universal holder
• 1x centering telescope (CT)
• 1x C-mount photo adapter
• 1x Nexcope power cord
• 2x spare fuses
• 4x different hexagonal screwdrivers
• 1x Dust cover

6.5. Optical System (NIB610/NIB620/NIB630)
6.5.1. Infinity optical system
The NIB610/NIB620/NIB630 microscopes are equipped with a quintulpe nosepiece. It comes with a 4x planachromatic objective and 10x, 20x and 40x planachromatic phase contrast objectives. NIS infinity plan objectives can provide high contrast and very flat image up to wide field 22mm view.
With FN 22 wide field eyepieces, the optical system always brings you sharp, excellent resolution and high signal t noise ratio imaging.

6.5.2. Objective parameters

6.5.3. Intelligent operation system (NIB620/NIB630)
It can memorize the illumination brightness when using each objective.
When different objectives are converted to each other, the light intensity is automatically adjusted to reduce visual fatigue and improve work efficiency.

6.6. Electricity parameters

TROUBLE SHOOTING GUIDE OF NIB610/NIB620/NIB630

Under certain conditions performance of the unit may be adversely affected by factors other than defects. If problems occur, please review the following list and take remedial action as needed. If you cannot solve the problem after checking the entire list, please contact Bresser GmbH for assistance.

7.1. Optical system

nosepiece is not in the located position
(objective and light path not coaxial)| Locate the nosepiece pro-perly where it clicks
A lens (the objective, condenser, eyepiece or collector) is dirty.| Clean it thoroughly
Dirt or dust is visible in the field of view| A lens (the objective, condenser, eyepiece or collector) is dirty.| Clean it
Dirt/dust on the specimen| Clean it
Dirt or dust is visible in the field of view| Specimen is placed reversely| Turn it over
A lens (the objective, condenser, eyepiece or collector) is dirty.| Clean it
The aperture diaphragm is too large| Adjust the aperture diaphragm
Dirt or dust on the eyepiece| Clean it
The aperture diaphragm is too small| Adjust the aperture diaphragm
One side of image is blurred| Condenser is not properly centered| Center the condenser with the centering screw
The nosepiece is not properly engaged| Engage the nosepiece properly
The specimen is not clamped| Clamp it with stage clips
The image moves while focusing on the zoom| Specimens float on the
surface of the platform| Placed it stably
The nosepiece is not properly engaged| Engage the nosepiece properly
The brightness is not enough| The aperture diaphragm is too small| Adjust the aperture diaphragm

7.2. Mechanical system

reversely.
Cover slip is too thick| Turn it over;
Use standard cover glass with thickness of 0.17mm
The objective will touch the specimen when it is converted from low to high| Specimen is placed reversely;
Cover slip is too thick| Turn it over;
Use standard cover glass with thickness of 0.17mm
The specimen did not move smoothly| The specimen holder is not securely fastened| Make sure it is securely fastened
Field of view of one eye does not match that of the other| Interpupillary distance is incorrect| Adjust interpupillary distance
Eyestrain| The interpupillary distance does not adjust| Adjust the interpupillary distance correctly
The illumination is not appropriate| Adjust brightness control knob

7.3. Electrical system

the specified bulb
The voltage is too low| Raise the voltage

WARRANTY

The regular guarantee period is 2 years and begins on the day of purchase.
To benefit from an extended voluntary guarantee period as stated on the gift box, registration on our website is required. You can consult the full guarantee terms as well as information on extending the guarantee period and details of our services at www.bresser.de/warranty_terms.

NOTES/COMMENTS

Exclusive distribution for Germany and BeNeLux: BRESSER GmbH
Nexcope Service
Gutenbergstr. 2
DE-46414 Rhede
Germany
www.bresser.de
[email protected]

References

• IBERIA.COM en United States - the best prices for Iberia flights - Iberia USA
• Bresser | Startseite | Expand Your Horizon mit BRESSER
• bresser.de/warranty_terms
• Bresser | Mikroskope | Expand Your Horizon
• Bresser | Nexcope NIB610 professionelles, inverses Labor-Mikroskop | Expand Your Horizon
• Bresser | Nexcope NIB620 professionelles, inverses Labor-Mikroskop mit Phasenkontrast | Expand Your Horizon
• Bresser | Nexcope NIB630 inverses Forschungsmikroskop mit kippbarer Beleuchtungseinheit | Expand Your Horizon

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