thermoscientific Sorvall ST 8 Small Benchtop Centrifuge Instruction Manual
- October 30, 2023
- Thermoscientific
Table of Contents
thermoscientific Sorvall ST 8 Small Benchtop Centrifuge
The Thermo Scientific Sorvall ST 8 / 8R Centrifuge is a laboratory equipment that complies with the European Union’s Waste Electrical & Electronic Equipment (WEEE) Directive 2012/19/EU. It is marked with the WEEE symbol.
Technical Specifications
The Thermo Scientific Sorvall ST 8 / 8R Centrifuge has the following technical data:
- Control panel: Sorvall ST 8 / 8R
- Pre-selecting speed/RCF: 24
- Running time pre-selection: 24
- Continuous operation: 25
Usage Instructions
Before using the centrifuge, read through the instruction manual carefully and follow the instructions to avoid expiration of the seller’s warranty. Follow these steps to use the centrifuge:
- Turn on the centrifuge by pressing the power button on the control panel.
- Select the desired speed/RCF and running time using the pre-selection buttons on the control panel.
- If you want to operate the centrifuge continuously, press the continuous operation button on the control panel.
- Load the rotor with the samples to be centrifuged.
- Close the lid of the centrifuge securely.
- Press the start button on the control panel to begin the centrifugation process.
- After centrifugation, turn off the centrifuge by pressing the power button on the control panel.
- Remove the rotor and samples from the centrifuge.
- Clean the centrifuge according to the cleaning intervals and instructions in the maintenance and care section of the instruction manual.
Refer to the system menu section of the instruction manual for more information about the control panel and refer to the rotor and accessories inspection section for information on checking and cleaning the rotor and accessories. Use the chemical compatibility chart to ensure that the samples are compatible with the rotor and accessories.
Thermo Scientific Sorvall ST 8 / 8R Centrifuge
Instruction Manual
50143327-i · 04 / 2022
WEEE Compliance
This product is required to comply with the European Union’s Waste Electrical
& Electronic Equipment (WEEE) Directive 2012/19/EU. It is marked with the
following symbol
Thermo Scientific
P
Preface
Before starting to use the centrifuge, read through this instruction manual
carefully and follow the instructions. Failure to follow the instructions and
safety information in this instruction manual will result in the expiration of
the sellers warranty.
Items Supplied
Item Thermo ScientificTM SorvallTM ST 8 / 8R Centrifuge Power Supply Cable Instruction Manual CD
Quantity 1 1 1 1
If any parts are missing, please contact the nearest Thermo Fisher Scientific representative.
Intended Use
The centrifuge is intended for the separation of liquid human specimens, such
as blood or urine, collected in IVD specimen receptacles. The centrifuge is
used in in-vitro diagnostic processes to support the collection of information
regarding diseases and other physiological or pathological states, such as
immunological or hematological screening (e.g. measurement of free
hemoglobulin). The semi-automated centrifuge is intended to be used in medical
laboratories by trained personal.
Thermo Scientific
Sorvall ST 8 / 8R Centrifuge | 5
Precautions
Precautions
WARNING Observe the safety instructions. Not following these instructions can cause damage. The centrifuge is to be used for its intended use only. Improper use can cause damages, contamination, and injuries with fatal consequences. The centrifuge should be operated by trained specialists only. In order to ensure safe operation of the Thermo Scientific Sorvall ST 8 / 8R Centrifuge, general safety regulations must be followed. Mind the regulations in your country.
CAUTION
Due to the air friction the temperature of rotor may raise significantly while
the centrifuge is spinning. Ventilated units will always lead to temperature
increase compared to ambient temperatures. Refrigerated units have limitations
in cooling capabilities. Displayed and set temperature can deviate from sample
temperature. Sample temperature might exceed critical temperature of your
application.
WARNING
The magnets built into the rotors can have a negative effect on active
implants, such as cardiac pacemakers. The magnets are mounted on the bottom of
the rotor. Always keep a distance of 20 cm between the rotor and the active
implant, as the product generates permanent magnetic fields. The magnetic
field strength at a distance of 20 cm is less than 0.1 mT, so there should be
no interference.
Set Up Conditions
WARNING Plug the centrifuge only into sockets which have been properly
grounded. Turn off the centrifuge at the main switch. The mains plug must be
freely accessible at all times.
Press the STOP key to shut down the centrifuge. Pull out the power supply plug
or disconnect the power supply in an emergency. As safety zone maintain a
clear radius of at least 30 cm around the centrifuge. Do not place any
dangerous substances within this security zone. Set up in a well-ventilated
environment, on a horizontally levelled and rigid surface with adequate load-
bearing capacity.
Preparation
WARNING It is the obligation of the operator to make sure, that the proper
protective clothing is used. Mind the “Laboratory
Biosafety Manual” of the World Health Organization (WHO) and the regulations
in your country. Do not make any changes to the mechanical components of the
rotor. Do not touch the electronic components of the centrifuge nor alter any
electronic or mechanical components. Use only with rotors which have been
properly installed. Follow the instructions on the Thermo ScientificTM Auto-
LockTM rotor exchange in section “Rotor Installation” on page 22.
6 | Sorvall ST 8 / 8R Centrifuge
Thermo Scientific
Precautions
Do not use rotors, buckets or accessories which show any signs of removed
protective coating, corrosion or cracks. Contact customer service for further
advice or inspections.
Use only with rotors which have been loaded properly. Never overload the
rotor. Always balance the samples. Use only rotors and accessories for this
centrifuge which have been approved by Thermo Fisher Scientific.
Exceptions to this rule are commercially available glass or plastic centrifuge
labware, provided they have been designed to fit rotor or adapter cavities and
are approved for the speed or the RCF value of the rotor. Make sure the rotor
is locked properly into place before operating the centrifuge. Implement
measures which ensure that no one can approach the centrifuge for longer than
absolutely necessary while it is running. If used for foodstuffs machinery,
for cosmetics or pharmaceutical products, only use closed or aerosol-tight
containers for centrifugation.
Hazardous Substances
WARNING Especially when working with corrosive samples (salt solutions, acids,
bases), the accessory parts and vessel have to be cleaned thoroughly. Do not
centrifuge explosive or flammable materials or substances. The centrifuge is
neither inert nor protected against explosion. Never use the centrifuge in an
explosion-prone environment. Do not centrifuge toxic or radioactive materials
or any pathogenic micro-organisms without suitable safety
precautions.
If centrifuging any hazardous materials mind the “Laboratory Biosafety Manual”
of the World Health Organization (WHO) and any local regulations. When
centrifuging microbiological samples from the Risk Group II (according to the
“Laboratory Biosafety Manual” of the World Health Organization (WHO)),
aerosol-tight biological seals have to be used. Look on the internet page of
the World Health Organization (www.who.int) for the “Laboratory Biosafety
Manual”.
For materials in a higher risk group, extra safety measures have to be taken.
If toxins or pathogenic substances have contaminated the centrifuge or its
parts, appropriate disinfection measures
have to be taken (“Disinfection” on page 35). Extreme care should be taken
with highly corrosive substances which can cause damage and impair the
mechanical stability of the rotor. These should only be centrifuged in fully
sealed tubes. If a hazardous situation occurs, turn off the power supply to
the centrifuge and leave the area immediately.
Operating
WARNING Never use the centrifuge if parts of its cover panels are damaged or missing. Never start the centrifuge when the centrifuge door is open. Do not move the centrifuge while it is running. Do not lean on the centrifuge. Do not place anything on top of the centrifuge during a run.
Thermo Scientific
Sorvall ST 8 / 8R Centrifuge | 7
Precautions
Never open the centrifuge door until the rotor has come to a complete stop and
this has been confirmed in the display.
The emergency door release may be used in emergencies only to recover the
samples from the centrifuge, e.g. during a power failure (see section
“Mechanical Emergency Door Release” on page 39). Do not open the centrifuge,
while it is running.
In any case of severe mechanical failure, such as rotor or bucket crash, the
centrifuge is not aerosol-tight. In case of rotor failure the centrifuge can
be damaged. Leave the room. Inform customer service.
Maintenance
WARNING The centrifuge housing is not to be opened by the operator.
8 | Sorvall ST 8 / 8R Centrifuge
Thermo Scientific
Symbols used on the centrifuge
Symbols used on the centrifuge
This symbol refers to general hazards. CAUTION means that material damage
could occur. WARNING means that injuries or material damage or contamination
could occur.
This symbol refers to biological hazards. Observe the information contained in
the instruction manual to keep yourself and your environment safe.
This symbol refers to information on hazards, described within the manual.
This symbol demands to disconnect mains before transporting or servicing the
centrifuge.
This Symbol demands to check, if the rotor is installed correct by lifting it
slightly at the handle. See “Rotor Installation” on page 22.
Symbols used in the manual
This symbol refers to general hazards. CAUTION means that material damage
could occur. WARNING means that injuries or material damage or contamination
could occur.
This symbol refers to biological hazards. Observe the information contained in
the instruction manual to keep yourself and your environment safe.
Thermo Scientific
This symbol refers to electrical hazards.
Sorvall ST 8 / 8R Centrifuge | 9
1
Technical Specifications
Technical Data
Sorvall ST 8
Environmental Conditions
Environmental Conditions during Storage and Shipping Permissible Ambient
Temperature during Operation
Use in interior spaces Altitudes of up to 2000 m above sea level Max. relative
humidity 80 % up to 31 °C; decreasing linearly to 50 % relative humidity at 40
°C Temperature: -10 °C to +50 °C Humidity: 15 % to 85 %
+2 °C to +35 °C
Heat Dissipation1 Overvoltage Category
100 V, 50 / 60 Hz
120 V, 60 Hz
230 V, 50 / 60 Hz
0.31 kWh/h; 1060 Btu/h; 0.31 kWh/h; 1060 Btu/h; 0.31 kWh/h; 1060 Btu/h;
1120 kJ/h
1120 kJ/h
1120 kJ/h
II
Pollution Degree
2
IP
20
Running Time
99 h 59 min 50 sec, hold
Maximum Speed n max
Minimum Speed nmin Maximum RCF Value at n
max
Noise Level at Maximum Speed1
16000 rpm 300 rpm 24328 x g < 58 dB (A) (measured with a TX-150 rotor); < 61 dB (A) (measured with a MicroClick 24×2 rotor)
Maximum Kinetic Energy
8.12 kJ
Thermo Scientific
Dimensions
Height (open door / closed door) Width Depth
660 mm / 310 mm 370 mm 490 mm
Weight2
35 kg
1 Front Side Measurement, 1 m in front of the instrument at 1.6 m height 2 Without Rotor
Sorvall ST 8 / 8R Centrifuge | 11
Chapter 1 | Refrigerants
Sorvall ST 8R
Environmental Conditions
Environmental Conditions during Storage and Shipping Permissible Ambient
Temperature during Operation
Use in interior spaces Altitudes of up to 3000 m above sea level Max. relative
humidity 80 % up to 31 °C; decreasing linearly to 50 % relative humidity at 40
°C Temperature: -10 °C to +50 °C Humidity: 15 % to 85 %
+2 °C to +35 °C
Heat Dissipation1 Overvoltage Category
100 V, 50 / 60 Hz 0.35 kWh/h; 1170 Btu/h; 1140 kJ/h
II
120 V, 60 Hz
0.35 kWh/h; 1170 Btu/h; 1140 kJ/h
Pollution Degree
2
IP
20
Running Time
99 h 59 min 50 sec, hold
Maximum Speed n max
17850 rpm
Minimum Speed nmin
300 rpm
Maximum RCF Value at n max
30279 x g
Noise Level at Maximum Speed1 < 56 dB (A) (measured with a TX-150 rotor)
Maximum Kinetic Energy
10.1 kJ
Temperature Setting Range
-10 °C to +40 °C
220-230 V, 50 / 60 Hz
0.35 kWh/h; 1170 Btu/h; 1140 kJ/h
Dimensions
Height (open door / closed door) 700 mm / 320 mm
Width
460 mm
Depth
670 mm
Weight2
74 kg
1 Front Side Measurement, 1 m in front of the instrument at 1.6 m height 2 Without Rotor
Refrigerants
Article No. Centrifuge 75007203 Thermo Scientific Sorvall ST 8R 75007204 Thermo Scientific Sorvall ST 8R 75007205 Thermo Scientific Sorvall ST 8R
Refrigerant R-134a R-134a R-134a
Quantity 0.265 kg 0.315 kg 0.265 kg
Pressure GWP 26 bar 1430 21 bar 1430 26 bar 1430
CO e 2
0.38 t 0.45 t 0.38 t
Contains fluorinated greenhouse gases in a hermetically sealed system.
12 | Sorvall ST 8 / 8R Centrifuge
Thermo Scientific
Directives, Standards and Guidelines | Chapter 1
Directives, Standards and Guidelines
Region Europe 220-230 V, 50 / 60 Hz 230 V, 50 / 60 Hz
USA & Canada 220-230 V, 50 / 60 Hz 120V 60 Hz
Japan 100 V, 50 / 60 Hz China 230 V, 50 / 60 Hz 208-240 V, 50 / 60 Hz
Directive 98/79/EC In Vitro Diagnostics (EU) 2017/746* In Vitro Diagnostics
Medical Devices 2011/65/EU Directive on the Restriction of the use of certain
Hazardous Substances in electrical and electronic equipment (RoHS) Protective
goals of: 2006/42/EC Machinery 2014/35/EU Low Voltage 2014/30/EU
Electromagnetic Compatibility (EMC) FDA listed Product code JQC centrifuges
for clinical use Device class 1
CFDA listed
Standard EN 61010-1 EN 61010-2-020 EN 61010-2-101 EN 61326-2-6 EN 61326-1
Class B EN ISO 14971 EN ISO 13485
ANSI/UL 61010-1 UL 61010-2-020 UL 61010-2-101 EN ISO 14971 EN ISO 13485 IEC
61010-1 IEC 61010-2-020 IEC 61010-2-101 IEC 61326-2-6 IEC 61326-1 Class B EN
ISO 14971 EN ISO 13485
- dependent on EU implementation date
Thermo Scientific
Sorvall ST 8 / 8R Centrifuge | 13
Chapter 1 | Mains Supply
Mains Supply
The following table contains an overview of the electrical connection data. This data is to be taken into consideration, when selecting the mains connection socket.
Unit Article No.
Sorvall ST 8 75007202 75007200 75007201
Sorvall ST 8R 75007205 75007203 75007204
Mains Voltage
100 V ±10 % 120 V ±10 % 220-230 V ±10 % 100 V ±10 % 120 V ±10 % 220-230 V ±10 %
Frequency
50 / 60 Hz 60 Hz
50 / 60 Hz 50 / 60 Hz 60 Hz
50 / 60 Hz
Rated Current
5.5 A
5 A
2 A
10 A
8 A
4 A
Power Consumption 310 W
310 W
310 W
850 W
700 W
750 W
Equipment Fuse
10 AT
10 AT
5 AT
15 AT
15 AT
15 AT
Building Fuse
15 AT
15 AT
16 AT
15 AT
15 AT
16 AT
Rotor Selection
The Thermo Scientific Sorvall ST 8 / 8R Centrifuge is supplied without a rotor. Various rotors are available. If not stated otherwise the rotors can be used in the Thermo Scientific Sorvall ST 8 centrifuge as also in the Thermo Scientific Sorvall ST 8R centrifuge.
Part No. 75005701 75005702 75005703 75005704 75005705 75005706 75005723 75005721 75005600 75005709 75003623 75005715 75005719 75005720 75005733 75003694 75005765 75003602
Thermo Scientific Rotors TX-150 swinging bucket rotor TX-150 round buckets TX-150 50mL conical buckets TX100S clinical swinging bucket rotor with sealed carriers TX100 clinical swinging bucket rotor with carriers M10 microplate swinging bucket rotor M10 buckets M10 sealed buckets MT12 microtube swinging bucket rotor HIGHConic III fixed angle rotor CLINIConic fixed angle rotor MicroClick 24×2 microtube rotor MicroClick 30×2 microtube rotor 8×8 PCR Strip rotor Hematocrit rotor 8 x 50 mL Individually Sealed rotor (only usable in the Sorvall ST 8R) MicroClick 18×5 microtube rotor (only usable in the Sorvall ST 8R) Microliter 48 x 2 mL Rotor
For further details on rotor use and safety, additional accessories, adapters and spare parts, please refer to the rotor manual.
For more information visit our website at http://www.thermofisher.com/centrifuge
14 | Sorvall ST 8 / 8R Centrifuge
Thermo Scientific
2
Transport and Set Up
Before Setting Up
1. Check the centrifuge and the packaging for any shipping damage. Inform the
shipping company and Thermo Fisher Scientific immediately if any damage is
discovered.
2. Remove the packaging. 3. Check, if the items supplied are complete. Ssee
“Items Supplied” on page 5.
If the items supplied are incomplete, please contact Thermo Fisher Scientific.
Location
The centrifuge is only to be operated indoors. The set-up location must
fulfill the following requirements: A safety zone of at least 30 cm must be
maintained around the centrifuge.
People and hazardous substances must be kept out of this safety zone while
centrifuging. The supporting structure must be stable and free of resonance.
The supporting structure must be suitable for horizontal setup of the
centrifuge. The centrifuge is not to be exposed to heat and strong sunlight.
WARNING
UV rays reduce the stability of plastics. Do not subject the centrifuge,
rotors and plastic accessories to direct sunlight.
The set-up location must be well-ventilated at all times.
Transporting
WARNING
Always lift the centrifuge from both sides. Never lift the centrifuge by its
front panel, its back panel or at its door. Always remove the rotor before
moving the centrifuge.
Thermo Scientific
Sorvall ST 8 / 8R Centrifuge | 15
Chapter 2 | Leveling
Due to its weight, the centrifuge should be carried by at least two people.
Always lift the centrifuge at both sides. To prevent possible injuries at
least two people should lift and carry the centrifuge by holding it at the
bottom from opposite sides.
Transport the centrifuge and accessories upright within the associated
packaging, if possible.
NOTICE
Store the original centrifuge packaging. Contact a shipping company or the
customer service for the transport. Always remove the rotor before moving the
centrifuge. If you do not remove the rotor you might damage the centrifuge
drive or centrifuge spindle.
Leveling
CAUTION
If the centrifuge isn’t leveled, imbalances can occur and the centrifuge can
be damaged. Do not place anything under the centrifuge feet to level the
centrifuge. The centrifuge is to be placed on horizontal and level supporting
structures or benching. Hoirzontal level is to be checked when moving the
centrifuge to a new location.
Mains Connection
NOTICE
Plug the centrifuge into grounded electrical sockets only.
16 | Sorvall ST 8 / 8R Centrifuge
Thermo Scientific
1. Turn off the power supply switch.
Storage | Chapter 2
Power supply switch on the backside of the Sorvall ST 8 Power supply switch on
the right side of the Sorvall ST 8R
2. Check whether the cable complies with the safety standards of your
country. 3. Make sure that the voltage and frequency correspond to the figures
on the rating plate. 4. Establish the connection to the power supply with the
connecting cable.
Storage
WARNING
When removing the centrifuge and accessories from use if necessary clean,
disinfect or decontaminate the entire system. In doubt contact the Thermo
Fisher Scientific customer service.
Before storing the centrifuge and the accessories, it must be cleaned and if
necessary desinfected and decontaminated. Centrifuge, rotors, buckets and
accessories have to be thoroughly dried before storage.
Store the centrifuge in a clean, dust-free location. Be sure to place the
centrifuge on its feet. Avoid direct sunlight.
Shipping
WARNING
Before shipping the centrifuge and accessories you have to clean and if
necessary disinfect or decontaminate the entire system. In doubt contact the
Thermo Fisher Scientific customer service.
Before shipping the centrifuge please bear the following in mind: The
centrifuge must be clean and decontaminated. The decontamination must be
confirmed in a decontamination certificate. Contact customer service for more
details.
Thermo Scientific
Sorvall ST 8 / 8R Centrifuge | 17
Control Panel
3
The control panel contains the keys and displays of the centrifuge (only the
power switch is located on the right side (Sorvall ST 8R) or on the backside
(Sorvall ST 8) of the device).
Sorvall ST 8
1
2
3
4
56
11
10
No.
Function
1
Status
2
Speed / RCF Value
3
Running Time
4
Acceleration / Deceleration Profiles
5
PULSE Key
6
OPEN Key
7
STOP Key
8
START Key
9
Arrow Keys
10
TOGGLE Key for Speed / RCF Value
11
Program Keys
Thermo Scientific
9
87
Display Contents
The status of the centrifuge is displayed here.
The speed (rpm) or RCF value (x g) is displayed here.
The running time is displayed here.
Press the key multiple times to cycle through the available profiles.
Press the PULSE key to immediately start the centrifugation run and accelerate
up to the maximal permissible end speed (depending on the used rotor).
Releasing the key initiates a stopping process at the highest braking curves.
Press the OPEN key to activate the automatic door release (possible only when
device is switched on and when the rotor is fully stopped). “Mechanical
Emergency Door Release” on page 39
Press the STOP key to manually end the centrifugation run.
Press the START key to start a centrifugation run or to accept the current
settings.
Use these keys in order to modify the displayed value.
Use the TOGGLE key to change the display mode. (Speed / RCF Value).
Use the Program Keys to save and load programs. “Programs” on page 26
Sorvall ST 8 / 8R Centrifuge | 19
Chapter 4 | Sorvall ST 8R
Sorvall ST 8R
1
2
3
4
5
67
13
12
No.
Function
1
Status
2
Speed / RCF Value
3
Running Time
4
Temperature
5
Acceleration / Deceleration Profiles
6
PULSE Key
7
OPEN Key
8
STOP Key
9
START Key
10
SNOWFLAKE Key
11
Arrow Keys
12
TOGGLE Key for Speed / RCF Value
13
Program Keys
11
10
98
Display Contents The status of the centrifuge is displayed here. The speed (rpm) or RCF value (x g) is displayed here. The running time is displayed here. The temperature is displayed here. Press the key multiple times to cycle through the available profiles. Press the PULSE key to immediately start the centrifugation run and accelerate up to the maximal permissible end speed (depending on the used rotor). Releasing the key initiates a stopping process at the highest braking curves. Press the OPEN key to activate the automatic door release (possible only when device is switched on and when the rotor is fully stopped). “Mechanical Emergency Door Release” on page 39 Press the STOP key to manually end the centrifugation run. Press the START key to start a centrifugation run or to accept the current settings. Use the key for prewarming or precooling the centrifuge. Use these keys in order to modify the displayed value. Use the TOGGLE key to change the display mode. (Speed / RCF Value). Use the Program Keys to save and load programs. “Programs” on page 26
20 | Sorvall ST 8 / 8R Centrifuge
Thermo Scientific
4
Operation
NOTE Only display pictures of the Sorvall ST 8R centrifuge are shown. The
display of the Sorvall ST 8 is the same only missing the details for
temperature.
Switching on the Centrifuge
1. Turn on the power switch of the device. The device performs a self-check
of its software. a. When the centrifuge door is closed the display shows:
The speed and time displays read “0” and “00:00”; the current temperature
inside the rotor chamber is displayed.
b. When the centrifuge door is open the display shows:
The speed and time displays show the pre-set values ; the set temperature
inside the rotor chamber is displayed.
Open the Centrifuge Door
Press the OPEN key.
Close the Centrifuge Door
WARNING
Do not reach into the gap between the centrifuge door and the housing. Use the
emergency release only for malfunctions and power failures (see “Mechanical
Emergency Door Release” on page 39).
Thermo Scientific
Sorvall ST 8 / 8R Centrifuge | 21
Chapter 4 | Rotor Installation
Ensure that the centrifuge platform is clear from objects. Keep hands and
objects well clear of the underside and side of the centrifuge door when
closing. Close the centrifuge door by pressing down on it lightly in the
middle or on both sides of it. The centrifuge door mechanism will click and
lock in place. Lids should not be slammed as excessive force may cause damage
or disrupt samples.
NOTICE
The centrifuge door should audibly click into place.
Rotor Installation
The approved rotors for the Thermo Scientific Sorvall ST 8 / 8R are listed in
section “Rotor Selection” on page 14. Use only the rotors from this list in
the centrifuge. Permitted accessories are listed in the rotor manuals.
CAUTION
Unapproved or incorrectly combined accessories can cause serious damage to the
centrifuge.
The centrifuge is equipped with an Auto-Lock-locking system. This system is
used to automatically lock the rotor to the centrifuge spindle. The rotor does
not have to be bolted on to the centrifuge spindle. Proceed as follows: 1.
Open the centrifuge door and if necessary remove any dust, foreign objects or
residue from the chamber.
Auto-Lock and O-Ring must be clean and undamaged.
1 2
1
Auto-Lock
2
O-Ring
2. Place the rotor over the centrifuge spindle and lower vertically and slowly down the centrifuge spindle. If necessary a light push may be required to ensure connection. The rotor clicks automatically into place.
22 | Sorvall ST 8 / 8R Centrifuge
Thermo Scientific
Acceleration / Deceleration Rates | Chapter 4
CAUTION
Do not force the rotor onto the centrifuge spindle. If the rotor is very
light, then it may be necessary to press it onto the centrifuge spindle with
little pressure.
3. Check if the rotor is properly installed by lifting it slightly on the
handle or from beneath the rotor. If the rotor can be pulled up, then it must
be reconnected to the centrifuge spindle.
WARNING
If the rotor cannot be properly locked in place after several attempts, then
the Auto-Lock is defective and you are not permitted to operate the rotor.
Check for any damage to the rotor and the spindle: Damaged rotors must not be
used. Keep the centrifuge spindle area of the rotor clear of objects. If in
doubt please call Thermo Fisher Scientific Customer Service.
CAUTION
Check that the rotor is properly locked on the centrifuge spindle before each
use by pulling it at its handle or from beneath the rotor. The rotor has to be
locked tight.
4. If available close the rotor with the rotor lid. Check the according rotor
manual for further details on requirements and methods of closure.
CAUTION
Be sure to check all sealings before starting any aeorsol-tight applications.
See the information in the rotor instruction manual.
5. Close the centrifuge door.
Acceleration / Deceleration Rates
The Thermo Scientific Sorvall ST 8 / 8R offers you 2 profiles: standard and soft. The setting is displayed above the ACCELERATION / DECELERATION key.
Press the ACCELERATION / DECELERATION key to cycle through and set the available profiles. The LEDs show the chosen settings. The last profile is saved, if you restart the centrifuge.
LED Light Settings OFF SOFT ACC SOFT DEC SOFT ACC and SOFT DEC
Description Acceleration and Deceleration with max. Power = Standard Acceleration = Soft Deceleration = Soft Acceleration and Deceleration = Soft
Thermo Scientific
Sorvall ST 8 / 8R Centrifuge | 23
Chapter 4 | Acceleration / Deceleration Rates
Pre-Selecting Speed/RCF
RPM stands for Revolutions Per Minute. RCF stands for Relative Centrifugal
Force and allows better transfer of protocols between centrifuges and rotors
of differing size. Ensure that the rpm or RCF is correctly set. 1. Press the
TOGGLE key below the SPEED display to cycle through the rpm / RCF selection.
The LED light will indicate if “RPM” or “RCF” is selected. RPM / RCF can be
viewed during a run by pressing the toggle button. 2. Enter the desired value
by holding the arrow keys below SPEED in the corresponding direction, until
the desired value shows. First RPM / RCF will change in steps of 10. Holding a
key pressed will change the runtime then in steps of 100 and then in steps of
1000. Press the START key to accept or wait 4 seconds until the centrifuge
automatically saves the chosen values. Moving to setting time or temperature
also automatically stores the set value.
NOTICE
The minimum motor speed is 300 rpm. Any extremely low rcf settings will be
automatically increased to the minimum rcf at 300 rpm.
Explanation of RCF-Value
The relative centrifugal force (RCF) is given as a multiple of the force of gravity g. It is a unitless numerical value which is used to compare the separation or sedimentation capacity of various centrifuges, since it is independent of the type of device. Only the centrifuging radius and the speed are used for its calculation:
RCF = 11.18 x
x r
r = centrifuging radius in cm
n = rotational speed in rpm
The maximum RCF value is related to the maximum radius of the tube opening.
Remember that this value is reduced depending on the tubes and adapters used.
This can be accounted for in the calculation above if required.
Running Time Pre-Selection
1. Press the TIME arrow keys.This allows to change the set time using the
arrow keys until the desired time is displayed. First runtime will change in
steps of 10 second. Holding a key pressed will change the runtime by steps of
single minutes, followed by steps of 10 minutes, followed by steps of single
hours and at least by steps of 10 hours. This will continue until the limit of
99 hours and 59 minutes is reached. Enter the desired runtime in hh:mm or
mm:ss.
2. Press the START key to accept or wait 4 secondes until the centrifuge automatically saves the chosen values. Moving to setting speed / RCF or temperature also automatically stores the set value.
24 | Sorvall ST 8 / 8R Centrifuge
Thermo Scientific
Programs | Chapter 4
Continuous Operation
1. Press either arrow keys until HOLD is displayed. 2. Press the START key to
accept or wait 4 seconds until the centrifuge automatically saves the chosen
values. During
continuous operation, the centrifuge will continue running until you stop it
manually.
Preselecting the Temperature
You can preselect temperatures between -10 °C and +40 °C. To set the
temperature, proceed as follows:
Press the TEMPERATURE arrow keys. This allows to change the set temperature
using the arrow keys until the desired temperature is displayed. Temperature
will change in steps of single degrees celsius.
The temperature cannot be adapted until the rotor has been positively
identified; the speed display will then show END. When the rotor is not
recognized (centrifuge door closed and START key not yet pressed, speed
display “0”), the centrifuge ensures that the sample cannot freeze regardless
of the rotor being used.
Prewarming or Precooling the Centrifuge
Ensure the rotor, buckets and accessories are correctly in place and securely
attached in the chamber. For setting the pretemp value for the centrifuge
proceed as follows: 1. Press the SNOWFLAKE key in order to open the
temperature selection menu.
The display shows the message “Pre-Temp”. 2. Enter the desired value by
pressing the TEMP arrow keys, until the desired value shows.
3. Press the START key.
4. The centrifuge motor will start and a specific speed defined by the rotor.
This improves air circulation within the chamber, resulting in improved
temperature control throughout the chamber and rotor. The rotor chamber is
cooled down or heated up to the preset temperature.
5. When the set temperature is reached, the centrifuge will beep and continue
to hold the temperature. Press the STOP key to end the prewarming or
precooling. The display shows the current temperature inside the rotor
chamber.
Thermo Scientific
Sorvall ST 8 / 8R Centrifuge | 25
Chapter 4 | Programs
Programs
The Thermo Scientific Sorvall ST 8 Centrifuge is able to save up to 4
programs; the Thermo Scientific Sorvall ST 8R is able to save up to 99
programs. It is only possible to save a program if the centrifuge is in
standstill. Loading or saving of programs is not possible if the centrifuge is
spinning.
Saving a Program
Modify the speed, time and temperature to the desired setting.
For Direct Access Programs 1, 2, 3
Press and hold the desired program key 1,2 or 3 for 4 seconds.
For Programs 4-99
1. Press the folder key for 4 seconds. Use the SPEED arrow keys, to scroll
through until the desired number is selected. 2. Press the START key to
confirm. 3. The program can now be named using up to 12 alphanumeric
characters. Use the SPEED arrow keys to scroll
through the characters. Use the TIME arrow keys to move left or right. 4.
Press the START key to confirm and save the program. To abort at any point
press the STOP key.
Selecting a Program
For Direct Access Programs 1, 2, 3
Press one of the direct access program keys 1, 2, 3.
For Programs 4-99
Press the folder key. Use the SPEED arrow keys to scroll through until the
desired program is selected.
Centrifugation
Maximum Loading
WARNING
Injuries with fatal consequences can occur when using substance mixtures with
a higher density than 1.2 at maximum speed .
Rotors can run at high speeds. Each rotor is specifically designed to run at its maximum speed with a defined load. For further details refer to the rotor manual supplied with the rotor.
The rotors are designed to work with substance mixtures with a density of up to 1.2 g/ml. Above this density or if total load is above the maximum weight the following steps should be taken: · Reduce the fill level. · Reduce the speed.
26 | Sorvall ST 8 / 8R Centrifuge
Thermo Scientific
Centrifugation | Chapter 4
Use the formula: Use the table or the formula:
nadm = admissible speed nmax = maximum speed Once the rotor has been properly
installed, the main switch turned on and the centrifuge door closed, you may
start centrifuging.
Use of Tubes and Consumables
Care should be taken to ensure that the tubes and bottles used in the
centrifuge are: Rated to or above the selected rcf to be spun at They are
being used at or above there minimum fill volume They are not being used above
their design life (age or number of runs) They are inspected for damage Refer
to the manufacturers data sheets for further information.
CAUTION
Make sure that the length and width of the tubes are fitting into the adapter
and cavities. Do not use tubes that are too short or too thick for the adapter
and the cavities.
Thermo Scientific
Starting the Centrifuge Run
Press the START key on the control panel. The centrifuge accelerates to the
pre-set speed with the time display active. If the speed setting is higher
than the maximum permissible speed or RCF-value for the particular rotor, then
after starting the display will show the message “Limit” followed by the
maximum RPM- or RCF-value of the inserted rotor once the centrifuge has been
started. Within 10 seconds of the error message it is possible to accept the
highest RPM / RCF of the inserted rotor by pressing START. The centrifuge will
then continue run for the set time and at the set temperature. If no action is
taken within 10 seconds, the centrifuge will continue to decelerate. A new
speed setting can be selected using the SPEED arrow keys and and has to be
confirmed by pressing the START key. If no action is taken, the centrifuge
will decelerate until stop, then the lid should be opened and the rotor
checked.
Imbalance Indicator
The centrifuge is fitted with an imbalance detector, to ensure safety. If an
imbalance is detected at speeds higher than approx. 300 rpm an error message
“Imbalance load” will be displayed. Imbalance at high speed may indicate a
tube breakage or leak or rotor crash. Therefore additional care should be
taken depending on the samples loaded. The run will terminate. Once the run is
complete, the rotor and load should be checked, ensuring that all buckets are
greased and can swing free and that the tubes are balanced by following the
rotor instruction manual. For information on troubleshooting, see
“Troubleshooting by Guide” on page 40
Sorvall ST 8 / 8R Centrifuge | 27
Chapter 4 | Short-term Centrifugation
Stopping the Centrifuge Run
With Pre-Set Running Time
If the run time is preset, the centrifuge will run at the selected speed until
the desired run time is reached. It will then automatically decelerate and
stop. Once stopped “RUN COMPLETED” will be displayed and if selected the
display will flash. Access to the chamber and rotor can be gained by pressing
the OPEN key. If selected the door will open automatically. You can also stop
the centrifuging program manually at any time by pressing the STOP key.
Continuous Operation
If you selected continuous operation (“” on page 24), you will have to stop
the centrifuge manually. Press the STOP key on the control panel. The
centrifuge will be decelerated at the set rate. The message “RUN COMPLETED”
will be displayed. After pressing the OPEN key, the centrifuge door will open
and you can remove the centrifuged samples.
Short-term Centrifugation
For quick centrifugation, the Thermo Scientific Sorvall ST 8 / 8R has a PULSE-
function. By holding down the PULSE key, spinning will start at maximum
acceleration rate, continue at maximum rotor speed until the key is released.
The rotor then decelerates at maximum rate. Preset values for acceleration,
deceleration and speed or RCF are disregarded for the PULSE function. The
centrifuge accelerates and brakes at maximum power. Any rpm or RCF entered
beforehand is overridden.
NOTICE
The centrifuge accelerates to maximum speed according to the rotor used.
Check carefully whether you have to maintain a certain speed for your
application. During the acceleration process, time is counted forwards in
seconds. The reading stays displayed until the centrifuge door is opened.
Removing the Rotor
To remove the rotor, proceed as follows: 1. Open the centrifuge door. 2. Grab
the rotor handle and fully depress the Auto-Lock button. At the same time,
pull the rotor vertically upwards and
remove it from the centrifuge spindle. Make sure not to tilt the rotor while
doing this.
28 | Sorvall ST 8 / 8R Centrifuge
Thermo Scientific
Aerosol-tight Rotors | Chapter 4
Aerosol-tight Rotors
For your protection when using an aerosol tight lid the rotor should only be
removed with the lid closed.
NOTICE
Rotors supplied with a lid for aerosol-tight applications come with a mandrel,
which belongs to the Auto-Lock. Be sure not to place the lid onto this mandrel
to prevent it from being damaged.
CAUTION
Skin can be pierced by sharp mandrel tip. Do not touch the mandrel.
Switch off Centrifuge
To turn off the centrifuge push the mains switch to “0”.
Thermo Scientific
Sorvall ST 8 / 8R Centrifuge | 29
5
System Menu
To get into the system menu, press and hold any key on the front panel turning
on the centrifuge. Keep this key depressed until system menu appears in
display. Navigate through the system menu using the TIME arrow keys.
Flowchart System Menu
The system menu can be navigated using the SPEED arrow keys. The shown entry
can be changed using the TIME and TEMP arrow keys. Press the START key to save
this edit and quit the system menu. Press the STOP key to quit the system
menu. Values shown at some entries in the picture below are only examples.
NOTICE
A total number of cycles completed on the centrifuge is counted, since
installation or a new main board was installed. Cycle counts should be
periodically recorded to help with the identification of the total number of
runs a rotor has completed.
Thermo Scientific
Sorvall ST 8 / 8R Centrifuge | 31
Chapter 5 | Flowchart System Menu
32 | Sorvall ST 8 / 8R Centrifuge
Thermo Scientific
6
Thermo Scientific
Maintenance and Care
Cleaning Intervals
For the sake of personal, environmental, and material protection, it is your duty to clean and if necessary disinfect the centrifuge on a regular basis.
Maintenance Rotor Chamber (Bowl) Rotor Accessories Filter Mat (Capacitor) Cabinet Ventilation Holes
Recommended Interval Daily or when polluted Daily or when polluted Daily or when polluted Every six weeks or when polluted Once per month Every six months
Basics
CAUTION
Not rated procedures or agents could deteriorate the materials of the
centrifuge and lead to malfunction. Refrain from using any other cleaning or
decontamination procedure than those recommended here, if you are not entirely
sure that the intended procedure is safe for the equipment. Use only approved
cleansers. If in doubt, contact Thermo Fisher Scientific.
Use warm water with a neutral detergent that is suitable for use with the
materials. If in doubt contact the manufacturer of the cleaning agents.
Never use caustic cleaning agents such as soap suds, phosphoric acid,
bleaching solutions or scrubbing powder. Remove rotor and clean bowl with a
small amount of cleaning agent, applied to a clean cloth. Use a soft brush
without metal bristles to remove stubborn residue.
Afterwards rinse with a small amount of distilled water and remove any excess
with absorbent towels. Use only disinfectants with a pH of 6-8.
Rotor and Accessories Inspection
After thoroughly cleaning rotors, they should be inspected for damage, wear
and corrosion.
Metal Parts
Ensure that the black protective coating is complete. It can be removed
through wear and chemical attack and can lead to unseen corrosions. Any signs
of corrosions, such as rust or white / metalic pitting, the rotor or
accessories should be immediately removed from service. Particular attention
should be taken with the bottom of buckets on swing out rotors
Sorvall ST 8 / 8R Centrifuge | 33
Chapter 6 | Cleaning
and tube cavities on fixed angle rotors.
Plastic Parts
Check for signs plastic crazing, fading, bruising or cracking.
CAUTION
Do not run any rotor or accessories with sign of damage. Ensure that the
rotor, buckets and accessories are within the service life and number of
cycles. It is recommend that you have rotors and accessories inspected yearly
as part of your routine service to ensure safety.
Cleaning
CAUTION
Before using any cleaning methods except those recommended by the
manufacturer, users should check with the manufacturer of the cleaning agents
that the proposed method will not damage the equipment.
Clean as follows: 1. Clean rotor, buckets and accessories outside of the
centrifuge bowl. 2. Separate all rotors, buckets, lids, adapters and tubes to
allow thorough cleaning. 3. Rinse rotor and all accessories with warm water
and a neutral detergent that is suitable for use with the materials. If
in doubt contact the manufacturer of the cleaning agents. Ensure grease on
rotor trunnions (pivot point for swinging buckets) is cleaned away. 4. Use a
soft brush without metal bristles to remove stubborn residue. 5. Rinse rotor
and all accessories with distilled water. 6. Place the rotors on a plastic
grate with their cavities pointing down, to allow to fully drain and dry. 7.
Dry all of the rotors and accessories after cleaning with a cloth or in a warm
air cabinet at a maximum temperature of 50 °C. If drying boxes are used, the
temperature must never exceed 50 °C, since higher temperatures could damage
the material and shorten the lifetime of the parts. Once clean and dry,
inspect the rotor and accessories. After cleaning, treat the entire surface of
aluminum parts including the cavities with corrosion protection oil
(70009824). Treat the bolt of the swing out rotor with bolt grease (75003786).
CAUTION
Drive and doo lock can be damaged by entering liquids. Do not allow liquids,
especially organic solvents, to get on the drive shaft, the drive bearings or
the centrifuge door locks. Organic solvents break down the grease in the motor
bearing. The drive shaft could lock up.
34 | Sorvall ST 8 / 8R Centrifuge
Thermo Scientific
Disinfection | Chapter 6
Cleaning the Filter Mat
It is recommended that you clean the filter mat (50141352) regularly every six
weeks. Depending on the environmental conditions it may be necessary to clean
it more often. How to clean the filter mat: 1. Unscrew the ventilation grid
placed on the right side of the centrifuge. 2. Remove the ventilation grid. 3.
Remove the filter mat. 4. Clean the filter mat by tapping off the dust. The
filter mat can be rinsed with water, if needed. Dry the filter mat
before usig it again. NOTE Moisture can damage electronics and lead to
additional damages at the centrifuge. Only use dry filter mats. 5. Place the
filter mat back on the capacitor. 6. Screw the ventilation grid onto the
centrifuge.
Disinfection
WARNING
Hazardous infection is possible when touching the contaminated rotor and
centrifuge parts. Infectious material can get into the centrifuge when a tube
breaks or as a result of spills. In case of contamination, make sure that
others are not put at risk. Disinfect the affected parts immediately.
CAUTION
Equipment can be damaged by inappropriate disinfection methods or agents.
Before using any cleaning or disinfection methods except those recommended by
the manufacturer, users should check with the manufacturer that the proposed
method will not damage the equipment. Observe the safety precautions and
handling instructions for the cleaning agents used.
Thermo Scientific
The rotor chamber and the rotor should be treated preferably with a neutral
disinfectant. Contact the Service Department of Thermo Fisher Scientific for
questions regarding the use of other disinfectants. For details check “Basics”
on page 33. Disinfect as follows: 1. Disinfect rotor, buckets and accessories
outside of the centrifuge bowl. 2. Separate all rotors, buckets, lids,
adapters and tubes to allow thorough disinfection. 3. Treat the rotor and
accessories according to the instructions for the disinfectant. Adhere
strictly to the given
application times. Be sure the disinfectant can drain off the rotor. 4. Rinse
the rotor and accessories thoroughly with water and then rub down. 5. Place
the rotors on a plastic grate with their cavities pointing down, to allow to
fully drain and dry.
Sorvall ST 8 / 8R Centrifuge | 35
Chapter 6 | Decontamination
6. Dispose the disinfectant according to the applicable guidelines. 7. Clean
the rotor after disinfecting as described in “Cleaning” on page 34.
Decontamination
WARNING
Radiation is possible when touching the contaminated rotor and centrifuge
parts. Radioactive material can get into the centrifuge when a tube breaks or
as a result of spills. In case of contamination, make sure that others are not
put at risk. Decontaminate the affected parts immediately.
CAUTION
Equipment can be damaged by inappropriate decontamination methods or agents.
Before using any cleaning or decontamination methods except those recommended
by the manufacturer, users should check with the manufacturer that the
proposed method will not damage the equipment. Observe the safety precautions
and handling instructions for the cleaning agents used.
For general radioactive decontamination use a solution of equal parts of 70%
ethanol, 10% SDS ( Sodium Dodecyl Sulfate) and water. Decontaminate as
follows: 1. Decontaminate rotor, buckets and accessories outside of the
centrifuge bowl. 2. Separate all rotors, buckets, lids, adapters and tubes to
allow thorough decontamination. 3. Treat the rotor and accessories according
to the instructions for the decontamination solution. Adhere strictly to the
given application times. Be sure the decontamination solution can drain off
the rotor. 4. Rinse the rotor first with ethanol and then with deionized
water. Adhere strictly to the given application times. Be sure the
decontamination solution can drain off the rotor. 5. Rinse the rotor and
accessories thoroughly with water. 6. Place the rotors on a plastic grate with
their cavities pointing down, to allow to fully drain and dry. 7. Dispose of
the decontamination solution according to the applicable guidelines. 8. Clean
the rotor after disinfecting as described in “Cleaning” on page 34.
Autoclaving
1. Before autoclaving clean rotor and accessories as described above. 2.
Place the rotor on a flat surface. Rotors and adapter can be autoclaved at 121
°C. The maximum permissible autoclave cycle is 20 minutes at 121 °C. Clean the
rotor before autoclaving and rinse it with distilled water. Remove all
accessories (tubes, adapters) from the rotor.
36 | Sorvall ST 8 / 8R Centrifuge
Thermo Scientific
Place the rotor on a flat surface.
NOTE
No chemical additives are permitted in the steam.
CAUTION
Never exceed the permitted temperature and duration when autoclaving.
Service of Thermo Fisher Scientific | Chapter 6
Service of Thermo Fisher Scientific
Thermo Fisher Scientific recommends having the centrifuge and accessories
serviced once a year by an authorized service technician. The service
technician checks the following electrical equipment suitability of set-up
site centrifuge door lock and safety system rotor fixation of rotor and
centrifuge spindle protective casing Before service, centrifuge and rotors
should be thoroughly cleaned and decontaminated to ensure full and safe
inspection can be completed. Thermo Fisher Scientific offers inspection and
service contracts for this work. Any necessary repairs are performed for free
during the warranty period and afterwards for a charge. This is only valid if
the centrifuge has only been maintained by an authorized Thermo Fisher
Scientific service technician.
Shipping and Disposal
WARNING
When removing the centrifuge and accessories from use for disposal you have to
clean and if necessary disinfect or decontaminate the entire system. In doubt
contact the Thermo Fisher Scientific customer service.
For the disposal of the centrifuge mind the regulations in your country.
Contact the Thermo Fisher Scientific Customer Service for the disposal of the
centrifuge. For contact information check the backpage of this manual or visit
www.thermofisher.com/centrifuge For the countries of the European Union the
disposal is regulated by the European Union’s Waste Electrical & Electronic
Equipment (WEEE) Directive 2012/19/EU. (“WEEE Compliance” on page 2) Mind the
information on transport and shipping. “Transporting” on page 15 and
“Shipping” on page 17
Thermo Scientific
Sorvall ST 8 / 8R Centrifuge | 37
7
Troubleshooting
Mechanical Emergency Door Release
During a power failure, you will not be able to open the centrifuge door with
the regular electric door release. A mechanical override is provided to allow
sample recovery in the case of an emergency. This is only to be used in
emergencies and after the rotor has come to a complete stop.
WARNING
Spinning rotor can cause serious injuries when touched. In case of power
outage the rotor can still be spinning. Do not open the centrifuge before the
rotor has stopped. Do not touch the spinning rotor. Do not brake the rotor
using hands or other tools.
Always wait until the rotor has come to a stop without braking. The brake does
not work when there is no current. The braking process lasts much longer than
usual. Proceed as follows: 1. Make sure the rotor has stopped (view port in
the centrifuge door). 2. Pull out the power supply plug. Keep the centrifuge
horizontal at all times. 3. Pull the release cord.
a. Sorvall ST 8 On the right side of the housing is one white plastic plug
which can be removed from the plate with a small flat screwdriver. Once the
plug is removed it will expose the release cord. Pull the release cord
attached to it to trigger the mechanical door release. The centrifuge door
will open and the samples can be removed. Open the centrifuge door.
Mechanical Emergency Door Release located on the right side
Thermo Scientific
Sorvall ST 8 / 8R Centrifuge | 39
Chapter 7 | Troubleshooting by Guide
b. Sorvall ST 8R On the bottom of the housing is one white plastic plug which
can be removed from the plate with a small flat screwdriver. It is located
below the front side of the centrifuge. Once the plug is removed it will
expose the release cord. Pull the release cord attached to it to trigger the
mechanical door release. The centrifuge door will open and the samples can be
removed. Open the centrifuge door.
Below front side
Mechanical Emergency Door Release on the downside
4. Push the cord back into the centrifuge and mount the plug. Reconnect the centrifuge once the power has been restored. Switch on the centrifuge. Press the OPEN key to have the centrifuge door locks operative again.
Troubleshooting by Guide
If problems occur other than those listed in this table, the authorized customer service representative must be contacted.
Error number
Error message
Troubleshooting
E-002;
Read Manual
Restart the centrifuge.
E-005; E-008;
If the error message appears again, inform the service.
E-010;
E-011;
E-012;
E-015;
E-016;
E-034;
E-036;
E-041;
E-048;
E-050;
E-051;
E-052;
E-053;
E-054;
E-072;
E-077;
E-101;
E-104
40 | Sorvall ST 8 / 8R Centrifuge
Thermo Scientific
Error number E-031
E-017; E-020; E-021; E-022; E-023 E-078; E-079; E-080; E-081; E-019
E-025; E-027
E-029; E-045
E-030
E-098
Thermo Scientific
Error message Temp High!
Read Manual
Rotor Unknown Read Manual Read Manual Power Failure Imbalance Load
Troubleshooting by Guide | Chapter 7
Troubleshooting CAUTION Hot metal parts! Check, if the centrifuge is
accessible. Be sure, that the room temperature is within the limits. Let the
centrifuge cool down for 15 minutes. Be sure there is no condensed water in
the rotor chamber. If the error message appears again, inform the service.
Wait until the rotor has stopped. Check, if the rotor is qualified for the
Sorvall ST 8 / 8R Centrifuge (check “Rotor Selection” on page 20). Check, if
the bottom of the rotor is damaged and if the rotor is placed on the Auto-Lock
correctly. If the error message appears again, inform the service.
Restart the centrifuge. Check, if the rotor is qualified for the Sorvall ST 8
/ 8R Centrifuge (check “Rotor Selection” on page 20). If the error message
appears again, inform the service. Check, if the centrifuge door is blocked.
Restart the centrifuge. If the error message appears again, inform the
service. Check, if a rotor is installed. Check, if the rotor is qualified for
the Sorvall ST 8 / 8R (check ,,Rotor Selection” on page 14). Restart the
centrifuge. If the error message appears again, inform the service. Check the
power supply of the centrifuge. Make sure not to operate too many devices at
one power source. Let the centrifuge cool down for 15 minutes. If the error
message appears again, inform the service. Check the load placed in the rotor.
Check that the rotor cross bolts are greased well. Restart the centrifuge. If
the error message appears again, inform the service.
Sorvall ST 8 / 8R Centrifuge | 41
Chapter 7 | When to contact Customer Service
Error number E-060
E-046 E-099
Error message Temp Low!
Door Open! Set Speed Too High
Troubleshooting CAUTION
Icy metal parts!
Restart the centrifuge.
If the error message appears again, inform the service. Restart the
centrifuge.
If the error message appears again, inform the service. The installed rotor is
not rated for the programmed speed. Check the programmed speed.
When to contact Customer Service
If you need to contact customer service, please provide the order no. and the
serial no. of your centrifuge. This information can be found on the nameplate
at the back near the inlet for the power supply cable. In addition the
customer service also needs the Software ID and the NVRAM ID. Both are
available in the system menu.
42 | Sorvall ST 8 / 8R Centrifuge
Thermo Scientific
A
Chemical Compatibility Chart
MATERIAL ALUMINUM ANODIC COATING for ALUMINUM BUNA N CELLULOSE ACETATE BUTYRATE POLYURETHANE ROTOR PAINT COMPOSITE Carbon Fiber/Epoxy DELRINTM ETHYLENE PROPYLENE GLASS NEOPRENE NORYLTM NYLON PET¹, POLYCLEARTM,CLEAR CRIMPTM POLYALLOMER POLYCARBONATE POLYESTER, GLASS THERMOSET POLYTHERMIDE POLYETHYLENE POLYPROPYLENE POLYSULFONE POLYVINYL CHLORIDE RULON ATM, TEFLONTM SILICONE RUBBER STAINLESS STEEL TITANIUM TYGONTM VITONTM
Thermo Scientific
CHEMICAL 2-mercaptoethanol Acetaldehyde Acetone
Acetonitrile AlconoxTM Allyl Alcohol Aluminum Chloride Formic Acid (100%)
Ammonium Acetate Ammonium Carbonate Ammonium Hydroxide (10%) Ammonium
Hydroxide (28%) Ammonium Hydroxide (conc.) Ammonium Phosphate Ammonium Sulfate
Amyl Alcohol Aniline Sodium Hydroxide (<1%) Sodium Hydroxide (10%) Barium
Salts Benzene Benzyl Alcohol Boric Acid Cesium Acetate Cesium Bromide Cesium
Chloride
S SU / SMS / SUS SUS S / S S S SUS S S S S S S / U U / / / M / U / / / MU U
UMM / M S U / S / U M S U U S UM S S U U S U S U U U S S U U S MM S U U S S U
/ SMS / S S U S UMUU / SMUU S S S S UU UUS / S S S / S S S S S SMS S S S S S S
S S S SU / / / U / / S / / / / S / S SMS S S / MS / / S / / UU S S S S U S S S
SMS S S S / S S S S SMUU S S / SMU / / U / / / / U / SMUU S S / U S / U S / U
SSU / SSS / SSSSSSSU / SSSSSSSSSS
MSUS S S S S S S S S S SUU / S S S S S SMS S S
UU S U S SMS S S S S / S UMS S S S S S S S SMS
UU S U S UMS S S S S U S UMS S S S S S S S SMS
UUUU S UMS / S / S U S UU S S S / MS S S S / U
U / S / S S S S S S S S / S SM / S S S S S SMS S S UMS / S S U S S S S S S S S
S / S S S S S S U S S U S / MU / / S S / M / S / MS S S SM / / / U / S / M S S
UU S U SMS UUUUUUU / SMUU S S S S U S U / M S S S / / S M S S / S MM S S S S S
S M S S / U
U / MU / / U / MM S S U S U U S S S S S S M S S / U MU S / S S S S S S S S S S
SM / S S S S S SMS S S S S UU S UMU S UU S UUUMUMUUU S UU S U S S / U U / / MM
/ M / S U U U U U U U / M S M / S / S US SMS SUS S S S S S S S SUS S S S S S S
S S S M / S / S S S / S S S S / S S / / S S S S S SMS S S MS S / S S S / S S S
S S S S / / S S S S S SMS S S MS SUS S S / S S S S S S S / / S S S S S SMS S S
Sorvall ST 8 / 8R Centrifuge | 43
Annex A | Appendix
MATERIAL ALUMINUM ANODIC COATING for ALUMINUM BUNA N CELLULOSE ACETATE BUTYRATE POLYURETHANE ROTOR PAINT COMPOSITE Carbon Fiber/Epoxy DELRINTM ETHYLENE PROPYLENE GLASS NEOPRENE NORYLTM NYLON PET¹, POLYCLEARTM,CLEAR CRIMPTM POLYALLOMER POLYCARBONATE POLYESTER, GLASS THERMOSET POLYTHERMIDE POLYETHYLENE POLYPROPYLENE POLYSULFONE POLYVINYL CHLORIDE RULON ATM, TEFLONTM SILICONE RUBBER STAINLESS STEEL TITANIUM TYGONTM VITONTM
CHEMICAL Cesium Formate Cesium Iodide Cesium Sulfate Chloroform Chromic Acid
(10%) Chromic Acid (50%) Cresol Mixture Cyclohexane Deoxycholate Distilled
Water Dextran Diethyl Ether Diethyl Ketone Diethylpyrocarbonate
Dimethylsulfoxide Dioxane Ferric Chloride Acetic Acid (Glacial) Acetic Acid
(5%) Acetic Acid (60%) Ethyl Acetate Ethyl Alcohol (50%) Ethyl Alcohol (95%)
Ethylene Dichloride Ethylene Glycol Ethylene Oxide Vapor Ficoll-HypaqueTM
Hydrofluoric Acid (10%) Hydrofluoric Acid (50%) Hydrochloric Acid (conc.)
Formaldehyde (40%) Glutaraldehyde Glycerol Guanidine Hydrochloride Haemo-SolTM
Hexane Isobutyl Alcohol Isopropyl Alcohol
44 | Sorvall ST 8 / 8R Centrifuge
MS S / S S S / S S S S S S S / / S S S S S SMS S S MS S / S S S / S S S S S S
S / / S S S S S SMS S S MS S / S S S / S S S S S S S / / S S S S S SMS S S U U
U U S S MU S U UMUMU U UMMU U S U U UM S U / UU S UU / S S S U S SMUMS S UMSMU
S S S
U / UU / UU / / / S UU SMUMS S UMS / UM / S SSU / / / S / SUUUUUU / / UU /
USSSSUS S S S / S S S U S U S S U U UM S MUMM S UMMU S SSS / SSS / SSSSSSS / /
SSSSSSSSSS SSSSSSSSSSSSSSSSSSSSSSSSSSS MS S S S S S / S S S S S S S S S S S S
S S SMS S S S S UU S S S U S UU S UUUUUUUUU S S S SMU S / U U / / M / S U / S
/ MU U UMM / U S / / S U U S SU / S S S / S SUSUSU / / S S SMS S S S S S
SSUUSSS / SUSSUSUU / SSUUSSSSUU M S U U S S MM S U U S UMU U / MMMU S S S S U
U UU S / / / MS / M / S / S / / / S S / / / MU S / S S S UU S S UMS U S
UUUUUMS UMU S UU S / U S SMS S SMS S S S SMS S S S S S SMS SMS SM S S UU S S U
/ SMS UUMU SMSMSMSMU SMU MMU U S S MM S S U S UMU U / S S U U S MM S U U S S S
S S SMS S S S S U S U S S S S S S S SMSMU S S S U S SMS S S S S U S U / S S
SMS S S U SMU S / UU / / SM / UUSUUUUUUU / USU / S / S S S S S S S S S S S S S
/ SUS S S S S S S SMSMS S / U / / U / / SU / S / SM / / S S SUSUS S SU MS S /
S S S / S S S S / S S / S S S S S S SMS S S UUUM / / U / / UU S / SMU S S S
SMS UUU / /
U U U U / / U / / U U U U S U U U S S MM S U U U / M
UUUU / UUM / UMUUMUUU / S / U S UUU / /
MMM S S S S M S S S S M S S S U S S M S S S M S MU SSSS / / S / SSSSSSS / /
SSS / / SSS / / MS S / S S S S S S S S S S S S / S S S S S S S S S S UUS / SSS
/ SSSSSSS / / SSSSSSUSSS SSS / / / S / SSSSSSS / / SSSSSSSSSS S S S / S S S /
S S U S UMU S S U S SMS U S S U S / / MU / / S S / U / S U S SMS S S / S S S /
S / S MMMU S S S S S U S S U S UM S S S S S S S MMM S
Thermo Scientific
Appendix | Annex A
MATERIAL ALUMINUM ANODIC COATING for ALUMINUM BUNA N CELLULOSE ACETATE BUTYRATE POLYURETHANE ROTOR PAINT COMPOSITE Carbon Fiber/Epoxy DELRINTM ETHYLENE PROPYLENE GLASS NEOPRENE NORYLTM NYLON PET¹, POLYCLEARTM,CLEAR CRIMPTM POLYALLOMER POLYCARBONATE POLYESTER, GLASS THERMOSET POLYTHERMIDE POLYETHYLENE POLYPROPYLENE POLYSULFONE POLYVINYL CHLORIDE RULON ATM, TEFLONTM SILICONE RUBBER STAINLESS STEEL TITANIUM TYGONTM VITONTM
Thermo Scientific
CHEMICAL Iodoacetic Acid Potassium Bromide Potassium Carbonate Potassium Chloride Potassium Hydroxide (5%) Potassium Hydroxide (conc.) Potassium Permanganate Calcium Chloride Calcium Hypochlorite Kerosene Sodium Chloride (10%) Sodium Chloride (sat’d) Carbon Tetrachloride Aqua Regia Solution 555 (20%) Magnesium Chloride Mercaptoacetic Acid Methyl Alcohol Methylene Chloride Methyl Ethyl Ketone MetrizamideTM Lactic Acid (100%) Lactic Acid (20%) N/Butyl Alcohol N/Butyl Phthalate N, N-Dimethylformamide Sodium Borate Sodium Bromide Sodium Carbonate (2%) Sodium Dodecyl Sulfate Sodium Hypochlorite (5%) Sodium Iodide
S S M / S S S / S M S S M S S / M S S S S S M S S MM US S / S S S / S S S S S
S S S S S S / S S SMS S S MU S S S S S / S S S S S S U S S S S S S S S S S S S
USS / SSSSSSSSSSS / SSSSSSSUSSS UU S S S SM / S S S S / S U S S S S S S SMUMS
U
UUMU / / M / MS S / UMUUU SM / MU / UU / U
S S S / S S S / S S SUS S SM / SMSUS SMSUS MU S S S S S S S S S S S SMS / S S
S S S SMS S S M / U / S MM S / M / S / S M S / S S S M S MU S / S S S S / S S
S U S MU S UMM S / MMM S S U S S U S S / S S S S S S / / / S S S S S / S S S S
/ S SM / S
U / SUS S S / / / / S S S S S / S S / S / S SM / S
U UM S S UMU S U U S UMU S S MM S MMMMU S S U / UU / / U / / / / / UUUUUUU / /
/ / / S /M SSS / / / S / SSSSSSS / / SSS / SSSSSS MS S / S S S S S S S S S S S
S S S S S S S SMS S S
USU / SMS / SMSUUUU / SUUSMSUS S S S S S S U S SMS S S S S U S UMS S S S S S
SMSMU UUUUMS S U S UU S UUUUUMUUU S SMU S U S SUUS SMS SUUSUSUUUS SUUS S S SUU
MS S / S S S / S S S S / S S / / S S S S S SMS S S / / S / / / / / / MSU / S S
SMS S / MSMS S / S / / S S / / / / / MSM / S S S S S S SMSMS S / S S / S U / /
S / / S M / U S M S S S S MM S M / S / S S S U / S S S / S U U S U U UM / U U
S U S MM S U S
S S SUSMS / S SUSUSUU / S SUUSMS S SU
MS S S S S S S S S SUS S S S / S S S S S SMS S S US S / S S S / S S S S S S S
S / S S S S S SMS S S MU S S S S S S S S S S S S U S S S S S S S S S S S S
SSS / SSS / SSSSSSS / SSSSSSSSSSS
UUMS SMU S SMS S SMS S S SMS S SMU SMS MS S / S S S / S S S S S S S / / S S S
S S SMS S S
Sorvall ST 8 / 8R Centrifuge | 45
Annex A | Appendix
MATERIAL ALUMINUM ANODIC COATING for ALUMINUM BUNA N CELLULOSE ACETATE BUTYRATE POLYURETHANE ROTOR PAINT COMPOSITE Carbon Fiber/Epoxy DELRINTM ETHYLENE PROPYLENE GLASS NEOPRENE NORYLTM NYLON PET¹, POLYCLEARTM,CLEAR CRIMPTM POLYALLOMER POLYCARBONATE POLYESTER, GLASS THERMOSET POLYTHERMIDE POLYETHYLENE POLYPROPYLENE POLYSULFONE POLYVINYL CHLORIDE RULON ATM, TEFLONTM SILICONE RUBBER STAINLESS STEEL TITANIUM TYGONTM VITONTM
CHEMICAL Sodium Nitrate Sodium Sulfate Sodium Sulfide Sodium Sulfite Nickel Salts Oils (Petroleum) Oils (Other) Oleic Acid Oxalic Acid Perchloric Acid (10%) Perchloric Acid (70%) Phenol (5%) Phenol (50%) Phosphoric Acid (10%) Phosphoric Acid (conc.) Physiologic Media (Serum, Urine) Picric Acid Pyridine (50%) Rubidium Bromide Rubidium Chloride Sucrose Sucrose, Alkaline Sulfosalicylic Acid Nitric Acid (10%) Nitric Acid (50%) Nitric Acid (95%) Hydrochloric Acid (10%) Hydrochloric Acid (50%) Sulfuric Acid (10%) Sulfuric Acid (50%) Sulfuric Acid (conc.) Stearic Acid Tetrahydrofuran Toluene Trichloroacetic Acid Trichloroethane Trichloroethylene Trisodium Phosphate
SSS / SSSSSSSSSSSS / SSSSSUSSSS US S / S S S S S S S S S S S S S S S S S S SMS
S S S / S S / / / S / / / S S SUU / / S / / / S SM / S S S S / S S S SMS S S S
S SM / S S S S S S S S S S US S S S S / S S S / / S S S S / S S S S S SMS S S
S S S / / / S U S S S S UUMSMUU S S S U S S S S S / S / / / SMS S S SUS S S
SUS S S S / S SMS S / U S S S U U S U S S M S S S S S S S S S MU S MM UUMS S S
U S S S S S U S U S S S S S S S S UMS S
U / U / S U U / S MM / / MUM S MM / M S U / S / S
U U U / / U U / S UMU UMU U UMMUM S U U S U S
U S U / S MM / S UMU U S UM S M S U U S UMMM S U S U / S UM / S UMUUUUU S UMUU
S UUUMS
UUMS S S U S S S S U / S S S S S S S S S UMU S S
U UMM / / U S / M S U UMM S S S M S M S UMU / S
MS S S / / S / S S S S S S S S S S S S S S S S S S S
S S U / SMS S SMS U S S S U S S S S U S UMSMS U S UU S UU / U S S UUMUU / U
SMU S S UUUU MS S / S S S / S S S S S S S / / S S S S S SMS S S MS S / S S S /
S S S S S S S / / S S S S S SMS S S MS S / S S S S S S S S S S S S S S S S S S
S S S S S MS S / S S S / S S S S S SUS S S S S S S SMS S S UUSSSSS / SSSUSSS /
SSS / SSSUSSS USUS SUU / SUSU / S S S S S S S S SMS S S S U S UM S U U / S U S
U UMMUMMM S S S U S S M S U / UU / UU / / UUUUMUUUUMUU S U S S / S
UUMS S S U / S S S UU S U S S S S S S S S UMS S
U U U U S U U / S M S U UMU U S S S S M S MU UMM
MUU S S UU / S SMU S S S S S S S S S S UUU S S MUUU S UU / S SMUU S UUMS S S S
S UUUMS
MUUU / UUM / / MUU S UUUMS UMS UUU / S
S / S / / / S M S S S S / S S S S S S S S S MM S S S S S UU S UUMS UU S UUU /
MUUUU S U S S UU S S UU S SMU S UU S UUU S UMUUU S U S UUM UUU / S S UMS U S
UU SM / MS S UU S UUUMU S / U / / / MU / U / S UUUUUUUUU S U / S / S / / UU /
/ / U / U / SUUUUUUUUUSU / U / S
/ / / S / /M/ / / / / / S / / SSS / / S / / S / S
46 | Sorvall ST 8 / 8R Centrifuge
Thermo Scientific
Appendix | Annex A
MATERIAL ALUMINUM ANODIC COATING for ALUMINUM BUNA N CELLULOSE ACETATE BUTYRATE POLYURETHANE ROTOR PAINT COMPOSITE Carbon Fiber/Epoxy DELRINTM ETHYLENE PROPYLENE GLASS NEOPRENE NORYLTM NYLON PET¹, POLYCLEARTM,CLEAR CRIMPTM POLYALLOMER POLYCARBONATE POLYESTER, GLASS THERMOSET POLYTHERMIDE POLYETHYLENE POLYPROPYLENE POLYSULFONE POLYVINYL CHLORIDE RULON ATM, TEFLONTM SILICONE RUBBER STAINLESS STEEL TITANIUM TYGONTM VITONTM
CHEMICAL Tris Buffer (neutral pH) Triton X/100TM Urea Hydrogen Peroxide (10%) Hydrogen Peroxide (3%) Xylene Zinc Chloride Zinc Sulfate Citric Acid (10%)
USSSSSS / SSSSSSSSSSSSSSSSSSS SSS / SSS / SSSSSSSSSSSSSSSSSSS S / US S S S / /
/ / S S SMS S S S / S S SMS / S UUMS S UU / S S S U S S SMU S S S S S SMS U S
SMS S S / S / S S S S S S S SMS S S S S S S S S S S S U S S SMU S UUUUUUMUMUUU
S UMS U S UUSSSSUSSSSSSSSSSSSSSSSUSSS USS / SSSSSSSSSSSSSSSSSSSSSSS MS SMS SMS
S S S S S S S SMS S S S S S S S S S
¹ Polyethlyeneterephtalate
Key
S Satisfactory. M Moderate attack, may be satisfactory for use in
centrifuge depending on length of exposure, speed involved, etc.;
suggest testing under actual conditions of use. U Unsatisfactory, not
recommended. / Performance unknown; suggest testing, using sample to avoid
loss of valuable material.
NOTICE
Chemical resistance data is included only as a guide to product use. Because
no organized chemical compatibility data exists for materials under the stress
of centrifugation, when in doubt we recommend pretesting sample lots.
Thermo Scientific
Sorvall ST 8 / 8R Centrifuge | 47
Thermo Scientific
Index
A
Autoclaving 34
B
Before Setting Up 13
C
Centrifugation 24 Characteristics 9 Chemical Compatibility Chart 41 Cleaning
32 Cleaning Intervals 31 Close the Centrifuge Door 19 Continuous operation 22,
26 Control Panel 17
D
Decontamination 34 Directives, Standards and Guidelines 11 Disinfection 33
E
Entering Parameters 21
I
Imbalance 25 Intended Use 5 Items Supplied 5
L
Location 13
M
Mains Supply 12 Maintenance and Care 31 Maximum Loading 24 Mechanical
Emergency Door Release 37
O
Open the Centrifuge Door 19 Operation 19
P
Precautions 6 Precooling 23 Preface 5 Pre-Selecting Speed/RCF 22 Preselecting
the temperature 23 Pre-set running time 26 Prewarming 23 Programs 23
R
RCF-Value 22 Removing 26 Rotor Evaluation and Care of Your Rotor 35 Rotor
Installation 20 Rotor Maintenance 35 Rotor Selection 12
Running time pre-selection 22
S
Saving Programs 24 Service of Thermo Fisher Scientific 35 Shipping and
Deposing 35 Short-term Centrifugation 26 Sorvall ST 8 9 Sorvall ST 8R 10
Starting the Centrifuge Run 25 Storage 15 Switch on Centrifuge 19 Symbols 8
T
Technical Data 9 Technical Specifications 9 Temperature Adaptation during
Standstill 26 Transport and Set Up 13 Transporting the Centrifuge 13
Troubleshooting 37
W
WEEE 2
Sorvall ST 8 / 8R Centrifuge | 49
Thermo Scientific Sorvall ST 8
Thermo Electron LED GmbH Robert-Bosch-Straße 1 63505 Langenselbold Germany
Country of Origin: Thermo Fisher (Suzhou) Instruments Co., Ltd. No. 297 Taishan Road, New District, Suzhou, Jiangsu P. R. China
Thermo Scientific Sorvall ST 8R
Thermo Electron LED GmbH Robert-Bosch-Straße 1 63505 Langenselbold Germany
thermofisher.com/centrifuge
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50143327 is the original instruction manual.
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