YODN Hyper E340 Broadband Excitation Illuminator for Fluorescence Microscope User Manual

Broadband Excitation Illuminator for Fluorescence
Microscope
Hyper E340 User Manual

HYPER E340 User Manual (Edit 2023/03)

Product Introduction

The new generation Hyper E340 can effectively increase the LED light source intensity by 22% to 143% or more.
The Hyper E340 lighting system provides wide-range LED illumination with a spectral coverage range of 350 nm to 730 nm, which can satisfy the fluorescence commonly used in most experimental research, such as DAPI, GFP/FITC, cherry/Texas Red, Cy3, Cy5 (or Cy7). It can help you work efficiently and energy-starvingly.
The Hyper E340 lighting system comes with a comprehensive microscope adapter that can be applied to most existing microscopes or connected to a 3.0mm liquid light guide. The on/off and light source intensity adjustment of the light source output are controlled by the electronic control box and adjusted by the dimming knob.
In addition, the LED has a long lifespan, so you don’t have to worry about replacing bulbs. YODN Hyper E340 is the best choice for fluorescence microscopy research.

Cautions and Warnings:

Simple operation to ensure the normal operation of the light box.

Safety instructions:
Before operating the Hyper E340 lighting system, please read and follow all safety instructions provided. Failure to comply with safety instructions may result in fire, electric shock, or personal injury and may cause equipment damage or malfunction. Please keep all safety instructions.

Safety definitions:
Danger: Statements indicating a risk of death, serious injury, equipment damage, and procedures.
Warning: Statements indicating a risk of personal injury or procedures.
Notice: Statements indicating a risk of equipment damage or procedures.

Safety items:
Warning: Do not use unapproved power sources. Please use the YODN-provided power source for the Hyper E340 lighting system. The range of the external voltage input is 100~240 VAC, the voltage output is 12 VDC, and the maximum current output is 15.0 A.
Danger: Do not stare at UV bright light. The light source output of the lighting system includes UV light, which may cause harm to the eyes or skin. Do not stare at the light source output. To avoid exposure of eyes and skin to the beam, it is essential to wear goggles and protective clothing. The brightness of the lighting system is higher than that of most commercial lighting devices and is mainly used for coupling with microscopes or other biological analysis instruments.
Notice: Do not open the casing of the light box or change external devices in any way. Opening the lighting system casing will void the product warranty because there are no repairable or replaceable parts outside the lighting system.
Notice: Do not place liquid containers on top of the light box. The spilled liquid may damage the light box.
Notice: Do not drop or suddenly apply external force to the light box. The vibration caused by falling onto a hard surface, external impact, or collision may damage or displace the internal components of the lighting system, causing malfunction.
Note: The liquid light guide should not be subjected to impact or bending with a minimum bending radius greater than the standard value.

RISK GROUP 3
WARRING IR emitted from this product. Do not look at operating lamp CAUTION UV emitted from this product . Eye or skin irritation may result from exposure. Use appropriate shielding.

Illuminator System Accessories

1. DC Power Supply： GST160A12-R7B（YODN P/N：H006-0114311）。
2. AC Power Cord (optional according to region)：
   (1)H006-0067312 – Taiwan
   (2)H006-0067311 – North America
   (3)H006-0068311 – Europe
   (4)H006-0069311 – U.K.
   (5)H006-0070311 – West Germany
   (6)H006-0070311 – South Korea
   (7)H006-0071312 – China/Australia
   (8)H006-0372311 – Japan

3.2 Adaptor Optional according to the microscope model）：

3.3-Liquid light guide：Diameter (effective inner diameter)φ3 mm

3.4 Collimator:

Hyper E340 Function Introduction:

Illuminator：

Before operating the device, please ensure that the optical fiber is correctly installed into the optical fiber port. Installation method: remove the protective covers on both sides of the optical fiber, loosen the hand-twist screw, and slide the optical fiber completely into the optical fiber socket without interference. After inserting the optical fiber to the bottom position, gently tighten the hand-twist screw to secure the optical fiber without loosening, to prevent over-tightening and damaging the optical fiber.
The bending of the optical fiber must not exceed the specifications of the minimum bending radius (80mm or 3.2 inches). Excessive bending of the optical fiber may cause permanent deformation and reduce the amount of light output.
Note: Before turning on the light, please ensure that the protective covers on both sides of the liquid optical fiber have been removed, and the output end of the optical fiber is placed in a safe position, away from flammable and unprotected objects. The output light of the optical fiber (including UV light output) should not be directly viewed by human eyes to avoid high-intensity light and UV light radiation damage to the operator and others.

Illuminator installation and operation：
After turning on the power on the back of the Hyper E340 light source box, the light source box enters a standby state, and the startup switch displays “red.” Before starting the light source, make sure that the display panel shows an intensity of 000%, and then manually press the front panel startup switch to turn on or turn off the light source. Pressing the startup switch can turn on the light source. When the light source is turned on, the startup switch displays “blue,” and the output intensity is set to the minimum intensity of 000%. Use the front panel knob to adjust the intensity by rotating it (for example, 000% → 010% →… → 100%) or press the knob and rotate it to fine-tune the intensity (for example, 010% → 011% →… → 100%). The light source intensity is displayed on the panel.

Note: When the illuminator is turned on, take necessary protective measures to protect yourself and others from exposure to strong light (including UV light).
The illuminator does not have a safety interlock device. When the optical fiber is disconnected from the light source box during operation, the light source box continues to output light. Please take necessary protective measures to protect personnel and items from exposure to strong light (UV light).
Before turning off the illuminator, please make sure that the light source has been turned off. When the light source is turned off, the startup switch displays “red,” and the cooling fan runs for at least 3 minutes before turning off the power of the light source box.

Installation and operation：

1. Carefully remove the components from the cardboard box.

2. You can choose to use the Hyper E340 lighting unit through either manual control or remote Operation.

3. Remote operation – RS232: Connect one end of the USB cable to the host and the other end to the computer.

4. Remote operation – TTL: Connect via BNC in order to trigger the LED with an external switch.

5. Connect the power cord to the Power socket on the back of the host, and the other end to a power outlet. Once powered on, the blue light on the power supply will remain on.
   ※ Please ensure that the DC power supply provided with the product is used, otherwise it may damage the light source and void the warranty.

6. Liquid light guide installation method：
   a. Secure the light guide adapter to the light source lens port of the Hyper E340 unit.
   b. Attach the condenser lens to your microscope’s light receptacle.
   c. Remove the protective covers from both ends of the light guide, insert one end into the light guide adapter, and the other end into the condenser lens.
   d. After inserting the light guide into the port, gently tighten the screw by hand until the light guide is securely fixed in place. Over-tightening may cause damage to the light guide.

Operating instructions：

1. Manual control box – screen display：

1. Startup page

| LED On/Off status memory value
---|---

2 Main menu

3 Submenu -Local All

| LED On/Off status
4 LEDS energy sync adjustment：0~100%
---|---

4 Submenu -Local Channel

| CH1 UV LED intensity setting
CH2 Blue LED intensity setting
CH3 Green LED intensity setting
---|---
| CH4 Red LED intensity setting

5 Submenu -TTL All (TTL Functional status and ALL LEDs intensity setting)

| TTL Functional status (ON/OFF)
4 LEDS energy sync adjustment：0~100%
---|---

6 Submenu -TTL Channel ( LEDS Self-adjustment of energy)

| TTL Functional status (ON/OFF)
CH1 UV Trigger energy setting
CH2 Blue Trigger energy setting
CH3 Green Trigger energy setting
CH4 Red Trigger energy setting
---|---

7 Submenu -Information1

| CH1 UV LED temperature
CH2 Blue LED temperature
CH3 Green LED temperatureCH4 Red LED temperature
---|---

8 Submenu -Information2

| CH1 UV LED hours used
CH2 Blue LED hours used
CH3 Green hours used
CH4 Red LED hours used
---|---

9 Submenu -Version

| Firmware version
---|---

Remote Operation – USB:
Before starting the USB mode for the first time, you can go to YODN official website (https://www.yodnlighting.com/download/index/13) to download the driver and operating software.

YODN_EX40 Operating instructions:

Remote operation-TTL:

1. TLL Input specifications：
   a. Connector type: BNC (female port)
   b. Minimum low level : +0V
   c. Maximum low level : +1.5V
   d. Minimum high level : +4.0V
   e. Maximum high level: +5.5V
   f. Typical input current: 450 μA

2. BNC(TTL) Port：

Spectral output:

Hyper E340 light box spectrum is as follows:

Product specifications:

Relevant operating and storage environmental conditions and detailed specifications:

| Product Specification
---|---
1| Wavelength range| 350-730 nm
2| LED peaks/FWH| 365/20 nm, 460/40 nm, 560/80 nm, 630/20 nm; (Optional: 405/20 nm, 730/20 nm)
3| External power supply| Universal input 100-240VAC 50/60 H
4| Power consumption| 85W
5| LED on/off response| 1 ms
6| LED expected life| 25000 hours
7| Control Pad| LON/OFF
2.LED intensity control 10-100% 3.Color separation output control
8| External control| TTL Trigger & PC control
(RS232 command /USB B-type connector)
9| Dimension (mm)| Illuminator : 12/365211 Power supply :6716735
10| Weight| Approx. 5.5 Kg (including power supply)
11| Warranty| Illuminator : 24 Months
12| Option| 1.5M/y3 mm liquid light guide/ Adaptor, collimators
13| Certifications| CE(TBA)

Dimensions：

Daily Maintenance and Troubleshooting:

Daily Maintenance:
Please use a suction device to remove any accumulated dust or lint from the air intake/outlet of the device to ensure stable air supply for cooling. In dusty or smoky environments, it is recommended to clean the air intake/outlet with a suction device at least every 6 months.
The Hyper E340 lighting fixture does not have any parts or components that customers can replace themselves. Opening the outer casing of the lighting fixture will void the warranty.

Troubleshooting:
Only basic troubleshooting information is provided depending on the condition of the Hyper E340 lighting fixture. If the Hyper E340 lighting fixture needs repair, it can only be performed by authorized technicians.

Unable to Start:
a. Make sure the power cord is properly connected to the power outlet.
b. Make sure the power cord is properly connected to the power supply socket.
c. Make sure the power supply connector is properly connected to the Hyper E340 lighting fixture.
d. If the main power of the Hyper E340 lighting fixture is turned on, the blue light of the power supply should be constantly lit.

Low Light Intensity:
a. Remove the screw from the collimator lens by hand, insert the light guide into the collimator lens, and secure the light guide with the screw.
b. Make sure the collimator lens is compatible with the microscope and securely fix the collimator lens to the microscope.

Control Box Operation Failure: Please make sure the control box connector is securely installed and connected to the Hyper E340 lighting fixture.

Customer Service:

For technical support related to the product, please call +886-3-5637218, send an email to Sales@yodnlighting.com, or visit https://www.yodnlighting.com/contact/getInTouch to contact us.
You can also find relevant information on the YODN website at http://www.yodnlighting.com.

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http://weixin.qq.com/r/Ph2hubXEmujlrfHa90gs| https://www.yodnlighting.com/en

Warranty:

YODN offers a 2-year warranty for the Hyper E340 lighting fixture to end- users, starting from the date of shipment. The warranty for peripheral accessories of the lighting fixture does not cover liquid light guides.

HYPER E340 User Manual (Edit 2023/03)

Documents / Resources

| YODN Hyper E340 Broadband Excitation Illuminator for Fluorescence Microscope [pdf] User Manual
Hyper E340, Hyper E340 Broadband Excitation Illuminator for Fluorescence Microscope, Broadband Excitation Illuminator for Fluorescence Microscope, Excitation Illuminator for Fluorescence Microscope, Illuminator for Fluorescence Microscope
---|---

References

• yodnlighting.com
• yodnlighting.com/
• 與我聯繫-台灣優燈股份有限公司
• 軟/韌體 更新-台灣優燈股份有限公司

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