Impex Produkter Test 0-76 NANOCOLOR ortho- and total Phosphate 1 Instruction Manual
- June 3, 2024
- Impex Produkter
Table of Contents
Impex Produkter Test 0-76 NANOCOLOR ortho- and total Phosphate
Method
Photometric determination as molybdenum blue after acidic hydrolyzes and oxidation at 100120 °C The test is equivalent to the EPA method 365.3.
Range
Contents of reagent set:
20 test tubes total Phosphate 1
- 1 tube NANOFIX total Phosphate 1 R2
- 1 tube NANOFIX total Phosphate 1 R3
- 1 test tube with 5 mL total Phosphate 1 R4
Hazard warning
Information on hazards can be found on the outer label and on the safety data
sheet. The safety data sheet can be downloaded from www.mn-
net.com/SDS.
Preliminary tests
If the order of magnitude of the concentration in a sample is not known, a
preliminary test with QUANTOFIX® Phosphate (3–100 mg/L PO43–, REF 91320) or
VISOCOLOR® ECO Phosphate (0.2–5 mg/L PO4-P, REF 931084) rapidly gives this
information. From the order of magnitude the required dilution can be
calculated and prepared direc tly.
I nterferences
Precipitations after hydrolysis can be removed by membrane filtration prior to
the deter mination. If hig her amounts or organ ic com pounds and / or organic
phosphor us com pounds are pre sent, use NANOCOLOR® NanOx Metal (REF 918978)
for de composi tion. The following quantities of ions will not interfere: ≤ 2
mg/L As, NO2–, S2– (only ortho-P); ≤ 20 mg/L Fe, Cu, Cr; ≤ 100 mg/L Si, < 150
mg/L COD (reference to potassium hydrogen phthalate) The method ortho P can be
applied also for the analysis of sea water.
Procedure
Requisite accessories: piston pipette with tips
total Phosphate
NANOFIX R2, screw cap back on to test tube, shake. (Close NANOFIX tube immediately after use.) Place tube in heating block and start heating block. After 30 / 60 min remove test tube from heating block and allow to cool down to room temperature. Add NANOFIX R3 and 200 μL (= 0.2 mL) R4, mix. Clean outside of test tube and measure after 10 min.
ortho Phosphate
Filter sample solution. Open test tube, add 4.0 mL test sample (the pH value of the sample must be between pH 0 and 10), 1 NANOFIX R3 and 200 μL (= 0.2 mL) R4, screw cap back on to test tube, shake. Clean outside of test tube and measure after 10 min.
Notes
The concentration of condensed phosphates is the difference between total
phosphate without Phosphate R2 and ortho phosphate. Fast cooling of the
cells/cuvettes under cold water can lead to clot formation by the NANOFIX
capsules. Lower ortho phosphate concentrations (0.010–0.800 mg/L PO4-P) can be
determined by using semi-micro
cuvettes50 mm (REF 91950):
After 10 min pour the contents of the test tubes into semi-micro cuvettes 50 mm and measure [method 176x]. In analogy, also lower total phosphate concentrations can be determined.
Measurement
For MACHEREY‑NAGEL photometers see manual, test 0-76.
Measurement when samples are colored or turbid:
For all MACHEREY‑NAGEL photometers see manual, use key for correction value.
Photometers of other manufacturers:
For other photometers check whether measurement of round glass tubes is
possible. Verify factorfor each type of instrument by measuring standard
solutions.
Analytical quality control:
NANOCONTROL ortho Phosphate (REF 92576) or NANOCONTROL Multistandard Sewage
outflow 2 (REF 925010)
Disposal
Information about disposal can be found on the safety data sheet. The safety data sheet can be downloaded from www.mn‑net.com/SDS.
MACHEREY-NAGEL GmbH & Co. KG
Neumann-Neander-Str. 6–8 · 52355 Düren · Germany
Tel.: +49 24 21 969-0 · Fax: +49 24 21 969-199
[email protected]
www.mn-net.com