Labnet BioPette Plus Multichannel Pipettors Instruction Manual

Labnet BioPette Plus
Single-channel, 8-channel,
and 12-channel Pipettors
Instruction Manual

This manual is available in additional languages at www.labnetlink.com.

Product Description

The BioPette Plus single-channel pipettor is a volumetric instrument designed to measure and transfer liquids precisely and safely. It is available in volumes from 0.1 μL to 10,000 μL.
The BioPette Plus 8-channel and 12-channel pipettors are designed for microplate filling. The pipettors enable precise and simultaneous delivery of 8 or 12 preset volumes of liquid. These pipettors are available in four volume ranges: 0.5-10 μL, 5-50 μL, 20-200 μL, and 50-300 μL.

Single-channel pipettors

8-channel and 12-channel Pipettors

8-10
12-10| P4808-10
P4812-10| 0.5 – 10
---|---|---
8-50
12-50| P4808-50
P4812-50| 5 – 50
8-200
12-200| P4808-200
P4812-200| 20 – 200
8-300
12-300| P4808-300
P4812-300| 50 – 300

Labnet pipettors operate using an air-cushion (i.e., the aspirated liquid does not come into contact with the shaft or plunger of the pipettor). The liquid is drawn into the disposable tip attached to the pipettor.

Packing

The pipettors are delivered with the following:

Ejector cap (for pipettor models P3942-2,
P3942-10, P3942-5000, P3942-10000)| 1
Filters (for pipettor models
P3942-5000, P3942-10000)| 3

Filters
The 5,000 μL and 10,000 μL pipettors include a replaceable filter, fitted at the bottom of the shaft which prevents aspirated liquid from entering the shaft and contaminating
the inner surface and plunger. Use of the filter is particularly important when aspirating and dispensing large volumes of liquid. The filter should be replaced if it becomes wet.

Pipettor Design

Model Identification
The volume range of the pipettor is shown on the pushbutton and on the tip ejector bar in multi-channel pipettors.
Single- and Multi-channel Pipettors

A. Pipetting pushbutton: Consists of 2 parts: the pushbutton (A1) and the knob (A2).
B1. Volume adjustment knob: Used to set the volume.
B2. Locking Ring: Allows the volume setting to be locked by pushing the ring upwards.
C. Shaft: Manufactured using high quality plastic, ensuring high chemical and mechanical strength.
D. Tip ejector: For multi-channel pipettors, the tips are ejected sequentially, reducing the required force.
F. Shaft nut
N. Ejector pushbutton
R. Multi-channel manifold: Contains a set of plungers and a set of flexible- suspension shafts, which function to reduce the force required to attach the tip.

Safety Recommendations

Long-term use of the pipettor relies on correct method of use.
Please read and follow the instructions for use carefully.
Symbols used:
Danger, risk of injury.
NOTE Risk of damage to the pipettor or errors in pipetting.
NOTE:

• The pipettor is designed for the transfer of liquids only using the tip. Do not aspirate liquids without the tip attached. The aspirated liquid should not enter the pipettor, as it may cause damage.

• Single-use tips reduce the risk of contamination of samples.

• Keep the pipettor clean, avoiding the use of abrasive or corrosive cleaning agents (e.g., acetone).

• Keep the pipettor upright when there is liquid in the tip

• Only using the pipettor in accordance with the manufacturer’s instructions ensures the correct pipettor parameters are maintained.

• After replacing the plunger or the shaft, the pipettor should be calibrated.

• In the case of incorrect operation, the device should be cleaned in accordance with the Instructions for Use or transferred to a service point.

• Ambient operating temperature is +5°C to 45°C.

• Ambient storage conditions (in the original packaging during transport and short storage) is -25°C to 55°C.
  When working with the pipettor:

• Follow general work safety regulations regarding hazards related to work in the laboratory

• Take special care when pipetting aggressive substances.

• Use appropriate protective attire (e.g., clothing, goggles, and gloves).

• Avoid pointing the pipettor at yourself or others during use.

• Only use parts and accessories recommended by the manufacturer.

Specifications

The pipettor is a high quality instrument which offers excellent accuracy and precision. The accuracy and precision (repeatability) of the liquid volume depends on the quality
of pipet tips used. The values for accuracy and precision shown in the table below were obtained using manufacturer non-filter pipet tips. Those tips are recommended for use to ensure compatibility, accuracy and precision when pipetting.

Nominal
Volume
(4)| Volume
(IA)| Accuracy
(%)| Precision
. (%)| Non-filter
Tips (A
---|---|---|---|---
‘ Single-channel Pipettors| |
2| Min. 0.1| ±40.0| .12.0| 10
0.2| ±12.0| 6.0
1| ±2.7| 41.3
Max. 2| ±1.5| 0.7
10| Min. 0.5| ±4.0| .2.8| 10
1.0| ±2.5| ..1.8
5.0| ±1.0| 0.6
Max. 10.0| ±0.5| 0.4
20| Min. 2| ±3.0| .1.5| 200
10| ±1.0| 0.5
Max. 20| ±0.8| 0.3
100| Min. 10| ±1.6| 0.80| 200
50| ±0.8| .0.24
Max. 100| ±0.8| .0.20
200| Min. 20| ±1.2| .0.60| 200
100| ±0.8| 0.25
Max. 200| ±0.6| .0.20
1,000| Min. 100| ±1.6| ..0.40| 1,000
500| ±0.7| .0.20
Max. 1,000| ±0.6| 0.15
5,000| Min. 1,000| ±0.6| .0.25| 5,000
2,500| ±0.6| ..0.20
Max. 5,000| ±0.5| .0.15
10,000| Min. 1,000| ±2.5| 40.6| 10,000
5,000| ±0.8| .0.3
Max. 10,000| ±0.5| ..0.2
8 – 10
12 – 10| Min.| 0.5| ±10.0| ≤8.0| 10
---|---|---|---|---|---
| 1| ±8.0| ≤6.0
| 5| ±4.0| ≤2.0
Max.| 10| ±2.0| ≤1.2
8 – 50
12 – 50| Min.| 5| ±4.0| ≤2.5| 200
| 25| ±3.0| ≤1.2
Max.| 50| ±1.6| ≤0.6
8 – 200
12 – 200| Min.| 20| ±3.0| ≤1.5| 200
| 100| ±1.5| ≤0.8
Max.| 200| ±1.0| ≤0.6
8 – 300
12 – 300| Min.| 50| ±1.6| ≤1.5| 300
| 150| ±1.2| ≤1.0
Max.| 300| ±1.0| ≤0.6

The accuracy and precision were obtained gravimetrically using manufacturer tips performing at least 10 measurements of distilled water at a temperature of 20°C ± 1°C according to EN ISO 8655 standards.
The use of tips from other manufacturers or filter tips may result in incorrect liquid aspiration and require pipettor recalibration.
The design of the pipettor enables the user to recalibrate it according to the information presented in Section 9.

Pipettor Operation

Attaching the Tips

• Attach the correct tip corresponding to the model number displayed on the pipettor pushbutton (Section 5).
• Position the pipettor vertically when attaching tips.
• Single-channel pipettors: push the pipet tip on firmly using a slight twisting motion to ensure an airtight seal.
• Multi-channel pipettors: press the pipettor against the tips positioned in the rack box until the shaft retracts approximately 1.5mm into the manifold. The suspension system ensured even and airtight sealing of the pipet tips. The rocking motion does not have to be performed to seal the pipet tips tightly.

NOTE:

• Do not attach the tips with a rocking movement, as this may damage the shaft or plunger. Observe this rule particularly with single-channel pipettors of low volume range.
• Never draw liquids directly into the pipettor without the tip attached.

Adjusting the tip ejector
The tip ejector can be adjusted by the user and can accommodate most types of tip available on the market.
When using narrow tubes, it may be necessary to remove the tip ejector.
2-1,000 μL pipettors

1. Tip ejector disassembly
   • Press the tip ejector pushbutton.
   • Turn the tip ejector bush 1/3 turn counter-clockwise.
   • Slide the ejector from the plastic arbor.

2. Tip ejector assembly
   • Press the tip ejector pushbutton.
   • Line the tip ejector up with the plastic arbor.
   • Turn the tip ejector bush clockwise until it has fully latched.

3. Tip ejector adjustment
   • Remove the tip ejector pushbutton.
   • To increase the ejector length turn the plastic bush counter-clockwise.
   • To decrease the ejector length turn the plastic bush clockwise.

5,000 and 10,000 μL pipettors

1. Tip ejector disassembly
   • Remove the tip ejector pushbutton.
   • Using a screwdriver, turn the metal arbor counterclockwise to unscrew the ejector from the arbor.
   • Release the ejector.

2. Tip ejector assembly
   • Remove the tip ejector pushbutton.
   • Using a screwdriver, turn the metal arbor clockwise to secure the ejector and adjust its length.
   NOTE: Leave a space of at least 1mm between the shaft and tip ejector collar.

3. Tip ejector adjustment
   • Remove the ejector pushbutton.
   • Use the screwdriver to increase or decrease the ejector length.
   • Following ejector assembly or adjustment, ensure the ejector pushbutton is relocated.

If the tip ejector cannot be adjusted sufficiently, or if the diameter of the ejector is insufficient to eject the tip, it may be necessary to attach the ejector cap “M” to the ejector.
In 2 and 10 μL pipettors place the cap, supplied with the pipettor, on the bottom of the pipettor shaft and slide the cap upwards to the bottom of the tip ejector.

Volume Setting

1. To enable volume selection, set the locking ring to the lower position.
2. Aspiration volume can be set by either turning the pipetting pushbutton knob, or the adjustment knob. The volume setting is displayed as a three digit counter, which should be read from top to bottom. The smallest volume increment is printed on the bottom counter drum.
3. After the volume has been set, set the locking ring to the upper position to avoid inadvertent changing of volume.

Examples of counter indications
The decimal point in the volume of aspirated liquid (µL or mL) is indicated by change in colour of digits. Black digits represent integer numbers, and red digits represent decimal fractions (sizes up to 20 µL).

For maximum accuracy, the set volume must be approached from a higher value by decreasing counter readings. Before reaching the desired value, reduce the speed of turning the adjustment knob to avoid inadvertently exceeding the desired value.

Operating Instructions

Observing the following recommendations will ensure maximum possible accuracy and precision of liquid sampling.

• During operation, the volume setting should be locked, with black adjustment knob in the lower position.
• Ensure smooth and slow operation of the pipettor.
• Immersion of the tip into the sample liquid should be kept to a minimum depth, which should remain constant during aspiration. The recommended immersion depths are given in the table below:

• The pipettor should be held in a vertical position.
• The pipet tip should be changed whenever the volume setting is altered, and when a different liquid is to be aspirated.
• The pipet tip should be changed if a droplet remains on the end of the tip from the previous pipetting operation.
• Each new pipet tip should be pre-rinsed with the liquid to be pipetted.
• Liquid should never enter the pipettor shaft. To prevent this:
  • Press and release the pushbutton slowly and smoothly.
  • Never turn the pipettor upside down.
  • Never lay the pipettor on its side when there is liquid in the tip.
• Never force the volume setting beyond the recommended limits.
• When pipetting liquids with a different temperature from the ambient temperature, it is recommended to pre-rinse the tip several times prior to use.
• Do not pipettor liquids with temperatures above 70°C.

NOTE: When pipetting acids or corrosive solutions which emit vapors, it is recommended to disassemble the shaft and rinse the plunger and O-ring with distilled water after
finishing the pipetting operation.

Aspiration and Dispensing Instructions

Aspirating Liquid

1. Press the pushbutton to the first stop point. Holding the pipettor vertically, immerse the pipet tip into the sample liquid to the recommended depth (for recommended values see Section 7). If the pipet tip is not immersed to the recommended depth or if the pipetting pushbutton is rapidly released, air may enter the pipet tip.
2. Release the pipetting pushbutton slowly and smoothly to aspirate the sample. Wait one second and then withdraw the pipet tip from the liquid.
   CAUTION: Do not touch the used tip.
   Dispensing Liquid
   3. Place the end of the pipet tip against the inside wall of the vessel at an angle of 10° to 40°. Press the pushbutton smoothly to the first stop. Wait one second.
   4. Press the pushbutton to the second stop to expel any remaining liquid. While keeping the pushbutton depressed, remove the pipettor from the vessel by drawing the pipet tip against the inside surface of the vessel.
   5. Release the pushbutton to its starting position.
   6. Eject the pipet tip by pressing the tip ejector pushbutton.

Remember to change the pipet tip whenever a different type of liquid is to be sampled.
Aspirating High-density Liquids When pipetting liquids of higher viscosity or lower surface tension than water (e.g., sera or organic solvents), a film of liquid may be formed on the inside of the pipet tip which may produce erroneous results. As the film remains relatively constant in successive pipetting operations with the same tip, this error can be eliminated by pre- rinsing the tip and allowing a film to form before transferring the first sample. This is achieved by aspirating a sample and dispensing it back into the same vessel. Allowing a film to form prior to sampling ensures optimal accuracy and repeatability.
This pre-rinsing operation should be repeated when the volume to be aspirated is changed or when a new pipet tip is used.
NOTE: Normally the degree of error resulting from viscous liquids is negligible if pipetting is performed slowly and carefully, however can be minimized further by holding the pipet tip in position for at least 2 seconds after aspiration to allow the liquid time to react to the change in pressure before it is dispensed.
If the above method does not result in accurate values, recalibrate the pipettor in accordance with Section 9.
It is recommended to record recalibration and correction values, in order to facilitate reverse calibration to a standard liquid.
Filters
The 5,000 μL and 10,000 μL pipettors include a replaceable filter (L), fitted at the bottom of the shaft which prevents aspirated liquid from entering the shaft and contaminating the inner surface and plunger. Use of the filter is particularly important when aspirating and dispensing large volumes of liquid. The filter should be
replaced if it becomes wet.

Checking Pipetting Accuracy Parameters and Pipettor Recalibration

The pipettors have been factory-calibrated using gravimetric methods with manufacturer pipet tips and distilled water, in accordance with ISO 8655 guidelines for the maximum (nominal) liquid volume drawn by the pipettor and for 10% of the maximum or minimum liquid volume according to the values given (Section 5).
The pipettors are designed to enable recalibration and adaptation to different pipetting techniques and liquid properties (e.g., temperature, density, and viscosity).
Periodic checks of the operation of the pipettor are recommended at least once per year. Frequency of checks should be increased depending on workload, sterilization or autoclave processes, and frequency of replacement of component parts.
If during pipettor operation the accuracy error (the difference between the real aspirated volume and the preset volume) exceeds the permissible value given in the table in Section 5, pipettor recalibration should be carried out.
Recalibration of the pipettor involves volume adjustment based on the value obtained by weighing. Recalibration of the pipettor is performed only for one liquid volume drawn by the pipettor.
The recommended volume for recalibration is 10% of the maximum (nominal) value or minimum volume depending on which of these values is greater. See table on page 16 for more details.
Parameters for Checking the Pipetting Accuracy
The pipetting accuracy is influenced by factors such as: tips used, characteristics of the pipetted liquid (density, viscosity), and operating conditions (ambient temperature, pressure).
To determine the accuracy error of the pipettor, the following conditions should be met:

• Ambient temperature and temperature of the pipettor, pipet tips, and liquid should be within the range of 20°C to 25°C and stabilized during weighing within ±0.5°C.
• Measurements should be conducted using distilled water.
• Balance sensitivity should be suitable for the volume “V” to be measured.

• When calculating the liquid volume aspirated by the pipettor, the conversion factor (Z) [μL/mg] for distilled water or a liquid with comparable density should be taken into account. Sample values of conversion factors are given in the following table.

Temperature
(°C)|
---|---
95| 101.3| 105
20| 1.0028| 1.0029| 1.0029
21| 1.003| 1.0031| 1.0031
22| 1.0032| 1.0033| 1.0033
23| 1.0034| 1.0035| 1
24| 1.0037| 1.0038| 1
25| 1.0039| 1.004| 1.004

See ISO 8655 for the full table of conversion factors (Z).
NOTE: Pipetting should be performed in accordance with guidance described in Sections 7 and 8.
Checking the Pipetting Accuracy Parameters

• Set the preset volume depending on the pipettor volume according to the following table.

| | | |
---|---|---|---|---
|
Nominal volume (µL)| Preset volume (μL)| Permissible values (μL)| Volume Change ΔV (μL) for a Turn of a Calibration Key by
---|---|---|---
1 Turn| 1 Increment

Single-channel Pipettors

8-channel and 12-channel Pipettors

• Perform 10 aspirations, and calculate the average value in [mg].
• Calculate the volume in [μL] by multiplying the value in [mg] by the conversion factor Z [μL/mg].

If the average aspirated volume exceeds the permissible values of the range, the pipettor should be recalibrated.
Pipettor Recalibration

1. Remove the pipetting pushbutton.
   CAUTION: The pipetting pushbutton consists of 2 parts: the knob and the pushbutton. After removal of the pushbutton, both parts are separated.

2. Holding the volume setting knob to prevent rotation, insert the calibration key into the calibration screw.

3. Turn the key clockwise to reduce the aspirated volume, or counter-clockwise to increase the aspirated volume. Use the values given in the above table to precisely adjust the volume.

4. Remove the key and replace the pipetting pushbutton by first replacing the knob, then the pushbutton.

Determine the average aspirated volume. The average volume should be within the permissible range given in the table. If the volume exceeds the values stated, the recalibration procedure should be repeated.
When pipetting liquids with physical properties considerably different from those of water, follow the guidance given in Section 5.
More information on the calibration procedure can be found at www.labnetlink.com.

Pipettor Maintenance

Depending on the applications and intensity of use, the pipettor requires periodic maintenance. The components exposed to corrosive vapors, such as shaft elements,
should be regularly checked and cleaned.
Do not use sharp tools for pipettor maintenance. It may cause damage to the device and affect the user’s safety.
Cleaning
External surfaces of the pipettor such as the pushbutton, ejector pushbutton, handgrip, shaft nut, and adjustment knob may be cleaned using a cloth dampened in isopropyl
alcohol. The remaining parts removed from the pipettor during pipettor disassembly may be washed with distilled water or isopropyl alcohol.
NOTE: Before using cleaning agents other than those recommended by the manufacturer, check the compatibility charts and consider chemical resistance of the following
plastics which form components of the pipettor: PP, PC, POM, PA, PPS, PVDF.
Sterilization Sterilization using an autoclave
The pipettor can be sterilized in an autoclave at 121°C for 20 minutes. Sterilization under other conditions may cause damage to the pipettor. It is recommended to:

• Unscrew the shaft nut slightly in the P3942-2/P39421000 pipettors, and unscrew the shaft slightly in the P3942-5000 and P3942-10000. After autoclaving these parts should be screwed tight again
• Set the locking ring to the lower (unlocked) position prior to sterilization.
• Sterilize the pipettors using an autoclave with an initial vacuum and drying cycle.
• After sterilization, the pipettor should be dried and cooled to room temperature.
• The shaft of the P3942-5000 and P3942-10000 models should be autoclaved without the filter.

Precision and accuracy should not alter if the pipetting processes including autoclaving are carried out as described in this manual. If a change in accuracy occurs, it is recommended to:

• Check the calibration of the pipettor after the first, third, and fifth autoclaving cycles and then after every 10 autoclaving cycles.

Ultra Violet (UV) Sterilization

The pipettors are UV resistant. The distance from the radiation source to the exposed element of the pipettor should be at least 50 cm. Prolonged or intense UV exposure can cause discoloration of pipettor parts but does not affect its performance.

Troubleshooting

If a problem is encountered during pipettor operation, use the following table to identify and eliminate the fault following the instructions provided. Replacement of parts
should be required only occasionally and should not be required with normal pipettor use.

Droplets of liquid remain
to the pipet ip.| The pipet tip is emptied L oo fast.
of pressing the| Decrease the speed pipettor pushbutton
The pipet tip wettability has increased due to extensive use.| Replace the tip with a new one.
Droplets
of air appear in the liquid aspirated
into the pipet tip.| The pipet tip immersion depth is too shallow.| Immerse the tip
to the recommended depth according to the instructions.
The pipet tip is incorrectly pressed onto the pipettor shaft.| Press the pipettor tip firmly onto the pipettor.
The tip is damaged or
worn out due to extensive use.| Replace the tip with a new one.
The pipettor incorrectly aspirates the liquid or the liquid drops out from the tip.| The pipettor tip is incorrectly pressed onto
the pipettor shaft.
.| Press the pipet tip firmly onto the
pipettor.
Problem| Cause| Solution
---|---|---
The pipettor incorrectly aspirates
the liquid
or the liquid drops out
from the tip.| The shaft surface is damaged or contaminated at the sealing site.| Clean the shaft or replace it with a new one.
The pipettor incorrectly aspirates
the liquid
or the liquid drops out
from the tip.| The plunger or the
0-ring is damaged due to prolonged aspiration of corrosive liquids.| Disassemble the shaft set; wash the shaft, the plunger and the seal 1 (Section 10: Cleaning). Replace the elements with new ones if necessary
Apply a small amount of lubricant onto
the plunger and reassemble the set in the correct order.
The inside of the pipettor is contaminated.
The sealing elements are not sufficiently lubricated.
Uneven
work of the pipetting set, the pipetting pushbutton gets blocked.| The inside of the pipettor is contaminated due to aspiration of corrosive substances.| Unscrew the shaft set, wash the parts. Replace the elements with new ones if necessary. Apply
a small amount
of lubricant on
the plunger and reassemble the set in the correct order.
The inside of the pipettor is contaminated due to the liquid entering the pipettor.
The sealing elements are not sufficiently lubricated, e.g. after repeated autoclaving procedures.
Incorrect aspiration.| Liquid with properties other than water (density, viscosity).| Calibrate the pipettor using the liquid which is to be pipetted.
Tips with a filter with increased flow resistance.| Calibrate the pipettor using the tips which are to be used for pipetting.

If the problem persists after carrying out the above steps, contact our regional representative.
Before returning the pipettor, please ensure the pipettor is completely free of any chemical, radioactive, or microbiological contamination which could pose a threat during transport and repair.

Spare Parts

Contact our Customer Service to inquire about availability of spare parts. Pipettor model and name of the part required should be specified. The most common parts are depicted below.
NOTE: The replacement of the plunger requires conducting the calibration procedure according to Section 9.

*Seal (J) used only in models 2, 5,000 and 10,000 µL

*Seal (J) used only in models 2, 5,000 and 10,000 µL

Limited Warranty

Corning Incorporated (Corning) warrants that this product will be free from defects in material and workmanship for a period of three (3) years from date of purchase.  CORNING DISCLAIMS ALL OTHER WARRANTIES WHETHER EXPRESSED OR IMPLIED, INCLUDING ANY IMPLIED WARRANTIES OF MERCHANTABILITY OR OF FITNESS FOR A PARTICULAR PURPOSE. Corning’s sole obligation shall be to repair or replace, at its option, any product or part thereof that proves defective in material or workmanship within the warranty period, provided the purchaser notifies Corning of any such defect. Corning is not liable for any incidental or consequential damages, commercial loss or any other damages from the use of this product.
This warranty is valid only if the product is used for its intended purpose and within the guidelines specified in the supplied instructions manual. This warranty does not cover damage caused by accident, neglect, misuse, improper service, natural forces or other causes not arising from defects in the original material workmanship. This warranty does not cover the O-ring, or shaft. Claims for transit damage should be filed with the transportation carrier.
In the event this product fails within the specified period of time because of a defect in material or workmanship, contact Corning Customer Service at: [email protected], visit www.labnetlink.com, or contact your local support office.
Corning Customer Service will help arrange local service where available or coordinate a return authorization number and shipping instructions. Products received without proper authorization will be returned. All items returned for service should be sent using prepaid postage in the original packaging or other suitable carton, padded to avoid damage. Corning will not be responsible for damage incurred by improper packaging. Corning may elect for onsite service for larger equipment.
Some states do not allow limitation on the length of implied warranties or exclusion or limitation of incidental or consequential damages. This warranty gives you specific
legal rights. You may have other rights which vary from state to state.
No individual may accept for, or on behalf of Corning, any other obligation of liability, or extend the period of this warranty.
For your reference, make a note of the model number, serial number, date of purchase, and supplier here.
Model No.
Serial No. ____
Date Purchased
Supplier _____
Warranty/Disclaimer: Unless otherwise specified, all products are for research use only. Not intended for use in diagnostic or therapeutic procedures. Corning makes no
claims regarding the performance of these products for clinical or diagnostic applications.
Product availability may vary per region.
For additional product or technical information, visit www.labnetlink.com or contact your local sales office.

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---|---

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