Labnet C0060 Spectrafuge 6C Compact Research Centrifuge Instruction Manual
- June 4, 2024
- Labnet
Table of Contents
Labnet C0060 Spectrafuge 6C Compact Research Centrifuge
Introduction
The Spectrafuge™ 6C is a small benchtop centrifuge designed for separation of various research samples and is supplied with a 6 x 15 mL rotor. Adapters are available for tubes smaller than 10 mL. The Spectrafuge 6C reaches speeds of up to 6,500 rpm/4,000 x g.
Safety Information
Before using the Spectrafuge 6C for the first time, please read this entire
manual carefully. If the equipment is used in a manner not specified by the
manufacturer, the protection provided by the equipment may be impaired.
Symbols and Conventions
- The following chart is an illustrated glossary of the symbols that may be used in this manual or on the product.
- The electrical warning indicates the presence of a potential hazard which could result in electrical shock.
CAUTION This symbol refers you to important operating and maintenance (servicing) instructions within the product Instruction Manual. Failure to heed this information may present a risk of damage or injury to persons or equipment.
Operation Safety Precautions
- Never use the centrifuge in any manner not specified in these instructions.
- Never operate the centrifuge without a rotor properly attached to the shaft.
- Never fill tubes while they are in the rotor. Liquid spillage may harm unit.
- Never put hands in the rotor area unless the rotor is completely stopped.
- Never move the centrifuge while the rotor is spinning.
- Never use solvents or flammables near this or other electrical equipment.
- Never centrifuge flammable, explosive or corrosive materials.
- Never centrifuge hazardous materials outside of a hood or proper containment facility.
- Always load the rotor symmetrically. Each tube should be counterbalanced by another tube of the same type and weight.
- Always locate the centrifuge within easy access to an electrical outlet.
- Always use only centrifuge tubes designed to withstand centrifugal forces of at least 4,000 x g.
- Always use a wrench to tighten rotor nut.
- Do not operate the centrifuge if any of the following conditions exist:
- The centrifuge has not been installed properly.
- The centrifuge is partially dismantled.
- Service has been attempted by unauthorized or unqualified personnel.
- The rotor has not been installed securely on the motor shaft.
- Rotors and accessories not belonging to the standard range are being used without permission being obtained from the manufacturer to use such rotors and/or accessories in the centrifuge.
- Exception: Centrifuge tubes, normally available in the laboratory.
- The centrifuge is located in an explosive atmosphere.
- Materials to be centrifuged are combustible and/or explosive.
- Materials to be centrifuged are chemically reactive.
- The rotor load is not properly balanced.
Specifications
Package Contents
Installation
The centrifuge should be installed on a rigid, even surface such as a stable laboratory bench, cabinet, etc. To guarantee sufficient ventilation, ensure that the centrifuge has at least 6 inches (15 cm) of free space on all sides, including the rear. The centrifuge should not be located in areas subject to excessive heat such as in direct sunlight or near radiators or the exhaust of a compressor, as a buildup of heat may occur within the chamber. Before operating the centrifuge, check that the power source corresponds to that on the manufacturer’s rating label, then connect the power cord to the centrifuge and the power source.
Installation of Rotors and Rotor Maintenance
Tube and tube adapter reference chart:
Rotor Maintenance
The rotor should be cleaned thoroughly after each use. Thorough cleaning must be performed when spinning samples containing phenol or phenol chloroform. Periodically inspect the rotor for dents, dings, scratches, discoloration and cracks If any damage to the rotor is found, discontinue use of the rotor immediately and replace.
Removing and Installing the Angle Rotor
- Remove the rotor screw from the motor shaft by turning the screw counterclockwise.
- Lift the rotor upward and remove from the centrifuge.
- Ensure that the motor shaft adapter remains on the motor shaft (Figure 1).
- Clean the motor shaft and motor shaft adapter (see figure 1).
- Place the rotor on the motor shaft (figure 1) and over the motor shaft adapter (see figure 1 and 2).
NOTE: Figure 1 and 2 are located on the following page.
When loading the rotor, refer to figure 3 (located on the following page). Loading in the pattern indicated will ensure a balanced load. Tubes to be loaded should be filled equally by eye. The differencein the weight between the tubes should not exceed 2-3 grams. A partially loaded rotor may be centrifuged if the loading scheme for balancing a rotor given in figure 3 is followed.
Figure 1. Chamber and motor shaft
- A. Motor
- B. Motor shaft adapter
- C. Motor Shaft
Figure 2. Bottom of angle rotor
Figure 3. Loading the rotor
Overloading the Rotor
The maximum load of the rotor and the maximum speed has been established by the manufacturer. Do not attempt to exceed these values. The maximum speed of the rotor has been measured for liquids having a homogeneous density of 1.2 g/mL or less. In order to centrifuge liquids with a higher density it is necessary to reduce the speed.
NOTE :
Failure to reduce the speed may result in damage to the rotor and centrifuge. The revised maximum speed can be calculated with the following formula:
- Reduced speed (nred) =
× max speed (nmax)
Example: Where the density of the liquid is 1.7, the new maximum speed would be calculated as follows:
- (nred) = × 6,500 = 5,461 rpm
If in doubt concerning maximum speeds, please contact the manufacturer for assistance.
Operation
The following accessories are available for the Spectrafuge™ 6C:
CAUTION: Never attempt to operate the centrifuge with rotors or adapters that show signs of corrosion or mechanical damage. Never centrifuge strongly corrosive materials that may damage the rotors or accessories.
Closing the Lid
After the rotor has been properly secured and loaded, close the centrifuge
lid, making sure that the interlock has been engaged.
Lid Release
Following a run, the centrifuge will display will show flashing “00”. This signals the end of a run and the lid can now be opened by pressing the “lid knob” (left). Note that the lid can not be opened until the display flashes “00” and the rotor has stopped.
CAUTION: Do not attempt to open the lid of any centrifuge until the rotor has come to a complete stop.
In the event of a power failure or malfunction, it may be necessary to open the lid manually.
- Disconnect the power cord from the wall socket.
- Remove the plastic plug, located on the left side of the unit, below the quick button.
- Pull the cord (attached to the plug) to open the lid lock manually.
Lid Lock
The centrifuge can be started only with the lid securely closed. Do not
attempt to open the lid until the end of run signal “00” is displayed.
Speed Selection
The speed (rpm) can be selected from 300 to 6,500rpm with the knob (right).
The set speed can be viewed at all times on the large LED display (right).
Selection of Operating Time and Momentary Operation
Time can be selected in half minute intervals from 0.5 to 10 minutes, and in one minute intervals from 11 to 30 minutes. Time can also be set to continuous/hold by turning the timer knob past the thirty minute position. This will display the continuous setting “On”. When the preselected time expires, the centrifuge will stop automatically. To stop the centrifuge prior to the expiration of set time, press the “Start/Stop” knob. The centrifuge may be operated manually by pressing and holding the “Start/Stop” knob. The centrifuge will continue to run as long as the knob is depressed.
Starting the Centrifuge
Once the time and speed have been set the centrifuge can be started by
pressing the “Start/Stop” knob. The centrifuge will then run for the specified
amount of time.
Service and Maintenance
The Spectrafuge™ 6C requires no routine maintenance other than the occasional routine cleaning. All repairs should be performed by authorized, qualified personnel only. Repairs performed by unauthorized personnel may void the warranty.
Cleaning the Centrifuge
Always keep the centrifuge housing, rotor chamber, rotor and rotor accessories
clean. All parts should be wiped down periodically with a soft cloth. For more
thorough cleaning, use a neutral cleaning agent (pH between 6 and 8) applied
with a soft cloth. Excessive amounts of liquid should be avoided. Liquid
should not come into contact with the motor. After cleaning, ensure that all
parts are dried thoroughly by hand or in a warm air cabinet (maximum
temperature 50°C).
Cleaning the Rotor
The rotor should be cleaned after each use. When spinning samples containing
phenol or phenol chloroform, the rotor should be cleaned immediately after
use. Should a spill of infectious materials occur within the rotor or chamber,
the unit should be disinfected. This should be performed by qualified
personnel with proper protective equipment.
Replacing Fuses
Check the fuse when it is recommended in the Troubleshooting Guide located in
this manual. The fuse holder is located in the power inlet on the rear of the
unit. Disconnect the power cord from the power inlet. Open the fuse holder
drawer by inserting a small screwdriver under the tab and prying it open.
Remove the innermost (operative) fuse from its retaining tabs and replace the
fuse if necessary. A spare fuse is located in the outermost chamber of the
fuse drawer. Replace only with a fuse of exactly the same value as the
original (Fuse type may be found in the Technical data section of this
manual).
Troubleshooting Guide
Determination of G-values
The centrifuging radius of the 15 mL rotor is 8.5 cm for round bottom tubes
and 8.2 cm for conical bottom tubes. See Section 6. for the correct radius
when using adapters and smaller tubes. The chart on the next page can be used
to determine g-values.
RELATIVE CENTRIFUGAL FORCE
Limited Warranty
Corning Incorporated (Corning) warrants that this product will be free from defects in material and workmanship for a period of two (2) years from date of purchase. CORNING DISCLAIMS ALL OTHER WARRANTIES WHETHER EXPRESSED OR IMPLIED, INCLUDING ANY IMPLIED WARRANTIES OF MERCHANTABILITY OR OF FITNESS FOR A PARTICULAR PURPOSE.
Corning’s sole obligation shall be to repair or replace, at its option, any product or part thereof that proves defective in material or workmanship within the warranty period, provided the purchaser notifies Corning of any such defect. Corning is not liable for any incidental or consequential damages, commercial loss or any other damages from the use of this product. This warranty is valid only if the product is used for its intended purpose and within the guidelines specified in the supplied instruction manual.
This warranty does not cover damage caused by accident, neglect, misuse, improper service, natural forces or other causes not arising from defects in original material or workmanship. This warranty does not cover motor brushes, fuses, light bulbs, batteries or damage to paint or finish. Claims for transit damage should be filed with the transportation carrier. In the event this product fails within the specified period of time because of a defect in material or workmanship, contact Corning Customer Service at: USA/Canada at 1.800.492.1110, outside the U.S. at +1.978.442.2200, visit www.corning.com/lifesciences, or contact your local support office. Corning’s Customer Service team will help arrange local service where available or coordinate a return authorization number and shipping instructions.
Products received without proper authorization will be returned. All items returned for service should be sent postage prepaid in the original packaging or another suitable carton, padded to avoid damage. Corning will not be responsible for damage incurred by improper packaging. Corning may elect for onsite service for larger equipment. Some states do not allow limitation on the length of implied warranties or the exclusion or limitation of incidental or consequential damages. This warranty gives you specific legal rights. You may have other rights which vary from state to state. No individual may accept for, or on behalf of Corning, any other obligation of liability, or extend the period of this warranty.
For your reference, make a note of the serial and model number, date of purchase, and supplier here.
- Serial No.
- Date Purchased
- Model No.
- Supplier
Equipment Disposal
According to Directive 2012/19/EU of the European Parliament and of the Council of 4 July 2012 on waste electrical and electronic equipment (WEEE), this product is marked with the crossed-out wheeled bin and must not be disposed of with domestic waste. Consequently, the buyer shall follow the instructions for reuse and recycling of waste electronic and electrical equipment (WEEE) provided with the products and available at www.corning.com/weee
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Warranty/Disclaimer: Unless otherwise specified, all products are for research use only. Not intended for use in diagnostic or therapeutic procedures. Corning Life Sciences makes no claims regarding the performance of these products for clinical or diagnostic applications. For additional product or technical information, visit www.corning.com/lifesciences or call 800.492.1110. Outside the United States, call +1.978.442.2200 or contact your local Corning sales office.
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