INTEGRA 158310 Vacusafe Aspiration System Instruction Manual

INTEGRA 158310 Vacusafe Aspiration System

Introduction

Intended use

This is a general-purpose laboratory instrument. Any use of this instrument in a medical or IVD setting is under the sole responsibility of the user.
VACUSAFE is a vacuum-based system for aspiration of non explosive aqueous solutions, such as biological buffers and media.
If the VACUSAFE is used in a manner not specified by INTEGRA Biosciences, the protection provided by the VACUSAFE may be impaired.

Safety notes

WARNING
Read these operating instructions carefully before use and pay particular attention to sections containing this symbol.

1. Do not carry out any conversions and alterations on the device.
2. Defective parts may only be replaced with original INTEGRA Biosciences spare parts according to the INTEGRA Biosciences operating or service instructions.
3. Do not use VACUSAFE in an atmosphere with danger of explosion. Also, do not aspirate highly flammable liquids such as acetone or ether.
4. When handling dangerous substances, comply with the material safety data sheet (MSDS) and with all safety guidelines such as the use of protective clothing and safety goggles.
5. Prolonged exposure of VACUSAFE to UV-light can cause discoloration and/or yellowing of the plastic housing. However, this will not affect the performance of the device in any way.
6. Excess spare parts, accessories and consumables (e.g. tubings, lids, plastic parts, rubber products, O-rings, filter) should be stored in a light protected place at ambient temperature to prevent premature material aging caused by prolonged exposure to UV-light.

Regardless of the listed safety notes, additionally applicable regulations and guidelines of trade associations, health authorities, trade supervisory offices, etc. must be observed. Please visit our website www.integra- biosciences.com on a regular basis for up to date information regarding REACH classified chemicals contained in our products.

Description of the device

Please determine which VACUSAFE model you have purchased as it will simplify the reading of these operating instructions. Find the part number of your model on the main packaging label and note it here: 158 .

Scope of delivery

• Base (housing with integrated bottle stand)
• Bottle
• Lid with openings for tubing
• Tubing and filter
• Cable for level sensor (models 158300, 158310)
• Mains adapter
• VACUBOY hand operator

Overview of the VACUSAFE

1. Housing

2. Bottle for liquid collection

3. Lid with openings for tubing

4. Bottle handle (except model 158300)

5. VACUBOY hand operator

6. Tubing for connection of hand operator to bottle

7. Tubing for connection of base to bottle

8. On/off switch with indicator lamp

9. Knob for vacuum regulation

10. Level sensor (models 158300 and 158310)

11. Atmospheric pressure return valve
    Rear view

12. Button for calibration of level sensor

13. Socket for level sensor cable

14. Mains connection socket

15. Filter port (air inlet)

16. Air outlet with screwed air outlet connector

Installation

Operating environment

The VACUSAFE has been designed for use in a laboratory. It shall be installed in a level, dry and dust-free location with a temperature of 5–40°C and a maximal (non-condensing) relative humidity of 80%.

Power supply

Insert the mains adapter cable into the VACUSAFE mains connection socket (14) and plug it into the power source.

WARNING

Use an original INTEGRA Biosciences mains adapter only (see “6.1 Specifications” on page 12 for voltage requirements)

Set-up

• Connecting the filter

Insert the hydrophobic filter into the filter port with the green dot facing the instrument (see “Rear view” on page 5) in order to guarantee that the hydrophobic side of the filter is facing the liquid collection bottle

WARNING

The VACUSAFE should never be used without a filter.

Connecting the tubing for the air outlet

1. If not installed, screw the air outlet connector (#158 427) on the air outlet of the pump (16).
2. Fix the silicon tubing, ID 4 mm (e.g. #158332), on the screwed air outlet connector.

Connection of the bottle and hand operator

1. Place the bottle without lid in the base of the instrument. Insert the bottle handle into the neck of the bottle by screwing it in place. Close the bottle with the lid fitted with tubing openings and make sure that the lid is screwed on tightly.
2. Connect one end of the blue tubing to the filter and the other end either to the fitting on the lid marked “PUMP” (model 158 320) or to the blue tubing coupling (models 158300, 158 310).
3. Connect the long transparent tubing to the fitting on the lid marked “HAND” (model 158320) or to the red tubing coupling (models 158300, 158 310). Insert the VACUBOY hand operator into the other end of the long tubing.

Connection and calibration of the level sensor

1. Plug the level sensor cable into its socket at the back of the instrument and connect it to the bottle lid (models 158300, 158310). Make sure that the sensor pins inside the lid are dry and unsoiled.
2. Switch on the VACUSAFE. A double beep confirms correct connection of the level sensor.
3. Press the “CALIBRATION” button on the back of the VACUSAFE base until a short beep is emitted.

In case of failed calibration (indicated by continuous beeping and blinking of the switch indicator lamp), repeat the calibration after cleaning the sensor pins inside the lid and checking that the level sensor cable is fully inserted in the lid and base.

Operation

Vacuum activation and notes on use

Switch on the VACUSAFE to start the vacuum pump (the switch indicator lamp lights). Wait a few seconds until the air from the collection bottle is evacuated. To increase or decrease aspiration speed, turn the knob for vacuum regulation to the right or left, respectively (see “2.2 Overview of the VACUSAFE” on page 5).
Aspirate liquid by pressing the green button of the VACUBOY hand operator fitted with a suitable tip. Check the level of liquid in the bottle frequently, especially when working without level sensor, and make sure that there is enough space left for the liquid that needs to be aspirated.
To open the bottle, first release the vacuum by opening the venting port on the lid (open the sealing screw or remove the sealing cap). Alternatively you can aspirate air with the VACUBOY. When closing the bottle for use, screw the lid on tightly. The seal in the lid needs to be adequately compressed in order to function. Also make sure the venting port is closed.
Prevent liquid or foam overflow from the collection bottle into the filter. In case the filter gets accidentally wetted or soiled, exchange it immediately or otherwise the retention of further liquid cannot be guaranteed. Note that the hydrophobic filter holds back aqueous solutions, whereas retention of apolar liquids is limited. To prevent the build-up of foam, we recommend the use of an anti-foaming agent (e.g. Antifoam A from Sigma).

WARNING
When working with dangerous substances, you must comply with the material safety data sheet (MSDS) and the additionally applicable regulations of trade associations, health authorities, supervisory offices, etc. In addition, when working with hazardous volatile substances or biohazardous agents, either place the VACUSAFE inside a biosafety cabinet or connect the instrument’s air outlet (16) to a safety ventilation by means of a tube and of a connector (Part No. 158427).

Use of the level sensor

When the VACUSAFE bottle is full, the level sensor prevents foam or liquid from overflowing and accidentally soaking filter and instrument. As soon as foam or liquid is detected, the pump switches off (models 158300, 158 310) to avoid overfilling of the bottle. Additionally, an audible alarm is activated and the switch indicator lamp starts blinking.
It is possible to deactivate the level sensor by simply disconnecting the sensor cable (confirmed by a double beep).
Instrument, sensor cable and bottle lid constitute an assembly and they must be calibrated to match one another (see “3.3.4 Connection and calibration of the level sensor” on page 8). If you have several lids, sensor cables and instruments and swap them, you have to recalibrate the newly formed assemblies. Depending on the degree of soiling of the sensor pins inside the lid, it may be necessary to clean them and to calibrate the level sensor periodically.

Troubleshooting

Maintenance

Cleaning

• WARNING

Always switch off the VACUSAFE and disconnect it from the mains when carrying out maintenance work.

Clean the VACUSAFE housing with a lint-free cloth lightly soaked with mild soap solution in distilled water or with a 70% solution of isopropanol or ethanol. Never use bleach (sodium hypochlorite) or other solvents.
It is recommended to change the hydrophobic filter regularly, at least once a year. Exchange the filter immediately if liquid has entered.
When working with solvents and other hazardous materials always rinse the hand operator and tubing with water followed by 70% ethanol.

Autoclaving and chemical sterilization

• Autoclaving

All parts contacting the liquid can be autoclaved: waste collection bottle, lid with tube fittings, silicone tubing and hand operator VACUBOY. When working with biohazardous material, disinfect the VACUBOY with a 70% solution of isopropanol or ethanol or a suitable disinfectant before disassembling for autoclaving. For disassembly please refer to the operating instructions VACUBOY. Recommendation regarding autoclaving: polypropylene bottle for at least 60 min, glass bottle and disassembled VACUBOY for at least 20 min at 121°C.
The bottle lid has to be always unscrewed and kept loosely on the bottle during autoclaving. The tubing couplings made of PVDF have to be always disconnected during autoclaving.
Silicone may become brittle after extensive autoclaving. Replace the tubings if they are damaged.

WARNING

It is not recommended to autoclave the VACUBOY regularly as premature material aging may occur.
The filter and the instrument itself cannot be autoclaved.

Chemical sterilization

Two methods for chemical inactivation of biohazardous agents are commonly used:

• A suitable disinfectant is added to the full collection bottle and left to react for the recom-mended time. Any disinfectant can be used, provided that it is compatible with the bottle material (glass or polypropylene).
• The disinfectant is added to the empty bottle so that the aspirated liquid gets sterilized as it accumulates in the bottle. Use disinfectants that are free of chlorine or other corro-sive agents, especially when corrosive vapors are generated and aspirated.

Equipment disposal

The VACUSAFE device must not be disposed of with unsorted municipal waste. Dispose the VACUSAFE device in accordance with the laws and regulations in your area governing disposal of devices.

Technical data

Specifications

• Vacuum range -300 to -600 mbar, continuously adjustable
• Flow rate Pump: 8 l/min (air); Aspiration: 17 ml/s (liquid, aspiration pipette)
• Dimensions (H x W x D) 530 x 180 x 320 mm
• Weight 3.4 kg
• Electricity supply Mains adapter input: 100–240 VAC, 50/60 Hz
• Device input: 17–19 VDC, 15 W
• Degree of protection IP21
• Ambient conditions Operation: 5 to 40°C, max. 80% RH (non-condensing) Storage: -10 to 40°C, max. 95% RH (non-condensing)

Chemical compatibility

The table below lists VACUSAFE parts that come into contact with the aspirated liquid or its aerosols and vapors, and rates the compatibility of these parts to a few of the chemicals commonly used in laboratories. To determine the compatibility of a component to a chemical not listed in the table, please consult one of the several tables available on the internet.
Note that the rating refers to soaking in the concentrated chemical; however, more relevant here is the attenuated effect resulting from indirect contact with the diluted chemical. It is recommended to test the compatibility of relevant components to a specific chemical prior to extensive use.
INTEGRA Biosciences AG does not warrant that the information in the table is accurate or complete and that any material is suitable for any purpose.

Technical data

Compatibility ratings (N/A = info not available):

A = good, no or minor effects (discoloration or slight corrosion).

B = fair, moderate effects (softening, swelling and loss of strength), not recommended for continuous use.

C = critical, not recommended.

| JAVEL (e.g. NaClO)| Ethanol EtOH| Hydrochloric acid

HCl

| Sodium hydroxide

NaOH

| Acetone| Dimethyl sulfoxide

DMSO

| Phenol PhOH| Acetic acid AcOH| Sodium carbonate

Na2CO3

---|---|---|---|---|---|---|---|---|---
Parts| Materials
VACUBOY| Plastic parts| POM| C| A| B| A| A| A| B| C| A
O-rings| FPM (Viton)| A| A| A| C| C| B| A| A| A
Valve spring| Stainless steel| B| A| C| B| A| A| A| C| A
Tubing| Silicone| A| A| C| C| C| A| C| B| A
Bottle| 4 L| Polypropylene| A| A| A| A| A| A| A| A| A
3 L| glass| A| A| A| A| A| A| A| A| A
Lid| Lid| Polypropylene| A| A| A| A| A| A| A| A| A
Lid seal| TPE| A| A| A| A| A| A| N/A| N/A| N/A
Quick couplings| PVDF| A| A| A| C| C| C| A| B| A
Coupling‘s o-ring| FPM (Viton)| A| A| A| C| C| B| A| A| A
Level detection pins| Stainless steel| B| A| C| B| A| A| A| A| A
Tubing| Silicone| A| A| C| C| C| A| C| B| A
Y-piece| POM| C| A| B| A| A| A| B| C| A
Filter| Membrane| PTFE| A| A| A| A| A| A| A| A| A
Housing| Polypropylene| A| A| A| A| A| A| A| A| A
VACUSAFE

base

| Pump head housing| PPS| A| A| C| A| A| A| A| A| N/A
Pump head membrane| FPM (Viton)| A| A| A| C| C| B| A| A| A

7 Accessories and consumables

(see “2.2 Overview of the VACUSAFE” on page 5 )

| 158625
Bottle stand| for 4L polypropylene bottle| 158630

158427

VACUBOY

VACUBOY hand operator with:

• 1-channel stainless steel adapter 40 mm
• 1-channel adapter with ejector for disposable tips (with locking)
• Rubber adapter for Pasteur pipettes

VACUBOY set consists of:

• VACUBOY hand operator
• Stand for hand operator
• 1-channel stainless steel adapter 40 mm
• 1-channel adapter for disposable tips/GripTips (with locking)
• 1-channel adapter with ejector for disposable tips (with locking)
• Rubber adapter for Pasteur pipettes
• 8-channel stainless steel adapter 40 mm
• 8-channel adapter with ejector for disposable tips (with locking)

VACUBOY aspiration adapters

• Rubber adapter for Pasteur pipettes 155505
• 1-channel stainless steel adapter 40 mm 155502
• 1-channel stainless steel adapter 150 mm 155522
• 1-channel stainless steel adapter 280 mm 155525
• 1-channel adapter for disposable tips/GripTips (with locking) 155504
• 1-channel adapter for disposable tips/GripTips (pack of 5) 159023
• 1-channel adapter with ejector for disposable tips (with locking) 155526
• 1-channel adapter with ejector for disposable tips 159026
• 1-channel adapter with ejector for GripTips 159027
• 4-channel stainless steel adapter 40 mm 155524
• 8-channel stainless steel adapter 40 mm 155503
• 8-channel adapter with ejector for disposable tips (with locking) 155520
• 8-channel adapter with ejector for disposable tips 159024
• 8-channel adapter with ejector for GripTips 159025
• Stand for VACUBOY hand operator 155501

Consumables Part No.

• Tubing for connection of hand operator to bottle, 1.8 m, silicone 158332 for connection of base to bottle, 0.7 m, silicone, blue 158331
• Filter for protection of vacuum source, 0.45 m, hydrophobic 158015
• Tubing set + filter comprises part no. 158 331, 158 332, 158 015 158342

Quick tubing couplings Material Part No.

• Coupling plug self-closing, with barbed fitting, white/blue PVDF 158416
• (linked to tubing) self-closing, with barbed fitting, white/red PVDF 158417
• Coupling socket self-closing, with O-ring, white/blue PVDF 158423
• (mounted on lid) self-closing, with O-ring, white/red PVDF 158424

References

• biosciences.com
• Your Reliable Partner For Productive Pipettes | INTEGRA

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